About
18
Publications
2,983
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
369
Citations
Introduction
My research is focused on bacterial genomics from different WGS data, especially Illumina and Nanopore, applied to the study of bacterial adaptation and antimicrobial resistance dynamics within a ‘One Health’ context. Thus, I have developed several projects about the ecology and evolution of multidrug-resistant bacteria belonging to the ESKAP(E) complex in different regions of the world.
Current institution
Publications
Publications (18)
Carbapenems are considered one of the most important last-resort classes of antibiotics, and the spread of carbapenem-resistant Enterobacterales (CRE) is a serious concern worldwide. From a One Health point of view, reports on CRE in companion animals are increasing, requiring attention regarding their role in maintenance and direct transmission to...
Background
Antimicrobial resistance (AMR) is a global threat. Monitoring using an integrated One Health approach is essential to detect changes in AMR occurrence.
Aim
We aimed to detect AMR genes in pathogenic and commensal Escherichia coli collected 2013–2020 within monitoring programmes and research from food animals, food (fresh retail raw meat...
Members of the Klebsiella pneumoniae species complex (KpSC) are opportunistic pathogens that cause severe and difficult-to-treat infections. KpSC are common in non-human niches, but the clinical relevance of these populations is disputed. Utilising 3,255 whole-genome sequenced isolates from human, animal and marine sources collected during 2001-202...
We report 579 hybrid genome assemblies (568 complete) of Klebsiella pneumoniae species complex isolates from human, animal and marine sources in Norway collected 2001-2020, belonging to six phylogroups including K. pneumoniae (n=493) and K. variicola (n=69) and 364 unique sequence types.
The npmA gene, encoding a 16S rRNA methyltransferase, confers resistance to all clinically available aminoglycosides, posing a significant threat to effective antibiotic therapy. Here, we investigated the distribution and mobilization mechanisms of npmA variants, npmA1 and npmA2, through an exhaustive analysis of 692,646 available bacterial genomes...
Antimicrobial resistance (AMR) mechanisms, especially those conferring resistance to critically important antibiotics, are a great concern for public health. 16S rRNA methyltransferases (16S-RMTases) abolish the effectiveness of most clinically used aminoglycosides, but some of them are considered sporadic, such as RmtE. The main goals of this work...
Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, and detection of antimicrobial resistance (AMR). Numerous published methods and protocols exist, but a lack of harmonisation has hampered meaningful comparisons between results produced by different meth...
Improvements in cost and speed of next generation sequencing (NGS) have provided a new pathway for delivering disease diagnosis, molecular typing, and detection of antimicrobial resistance (AMR). Numerous published methods and protocols exist, but a lack of harmonisation has hampered meaningful comparisons between results produced by different meth...
Antimicrobial resistance (AMR) is one the major threats to public health today, especially resistance to last-resort compounds for the treatment of critical infections, such as carbapenems and aminoglycosides. Innumerable works have focused on the clinical ambit of AMR, but studies addressing the impact of wastewater cycles on the emergence and dis...
Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evol...
Salmonellosis is a common subclinical infection in pigs and therefore apparently healthy animals may represent a reservoir of antibiotic-resistant Salmonella for humans. This study estimates and characterizes resistance to two classes of antimicrobials considered of the highest priority within the critically important antimicrobials for humans, i.e...
Objectives:
To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles.
Methods:
Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a m...
The carriage of two important pathogens of pigs, i.e. enterotoxigenic E. coli (ETEC) and Clostridioides difficile, was investigated in 104 cloacal samples from wild griffon vultures (Gyps fulvus) fed on pig carcasses at supplementary feeding stations (SFS), along with their level of antimicrobial resistance (AMR). E. coli was isolated from 90 (86.5...
Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however...
Objectives: The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the...
We studied the presence of the mobile colistin resistance gene mcr-1 in human, animal and environmental Enterobacteriaceae from Cumana (Venezuela) collected in 2015. mcr-1 was detected in 2/93 Escherichia coli from swine and human isolates resistant to colistin. Whole-genome sequencing and transformation experiments identified mcr-1 on an IncI2 pla...
