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Introduction
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Publications
Publications (115)
The objective of this study was to assess the ability of experimental advanced breeding clones as parental genotypes to transmit agronomic and quality traits to their progenies in breeding programs. A half diallel set of crosses (excluding reciprocals) with six parents was assayed in field trials for three years; four of the parents were Solanumtub...
Wheat is one of the most important cereals in terms of its role in the human diet. The distribution of the nutrients in wheat grains depends largely on their morphology, the bran fraction being the richest in minerals, thus developing important functions related to human health. The main purpose of this study was to point out the potential nutritio...
Spike lavender, Lavandula latifolia Medik., is a species of economic importance for its essential oil (EO). The purpose of this study was to estimate the effect of the variable climate and fixed factors such as soil and geographic location on EO yield and quality. The study material was collected in 34 populations from four different Spanish bioreg...
Wheat is an important cereal with a key role in human nutrition. In this study, dietary fiber (DF) and arabinoxylans of different durum (Triticum turgidum ssp. Durum L.) and bread (Triticum aestivum L.) wheat flours were analyzed in order to point out their potential nutritional and health claims allege according to the current European regulation...
Wheat landraces are currently being explored mainly as a source of allelic variation related to crop resilience and low-input adaptation. Characterization of their high- and low-molecular weight glutenin subunits can aid breeders to select as donor local materials those ensuring good end-use properties in the derived elite cultivars. By using prote...
A new technology for reducing wheat flour toxicity for celiac disease patients through the in situ formation of gluten-chitosan interlocked self-assembled supramolecular architecture was developed. To have a deeper insight into the microstructure of this new molecular organization and its impact on the dough properties, its small and large deformat...
Scope
The prevalence of celiac disease has increased since the last half of the 20th century and is now about 1% in most western populations. At present, people who suffer from celiac disease have to follow a gluten‐exclusion diet throughout their lives. Compliance to this restrictive diet is demanding and the development of alternative strategies...
Background: Wheat is one of the main foods of the human diet. It contains a protein complex, gluten, which is
primarily responsible for the rheological behavior of wheat flours. However, it is gluten that triggers and
maintains the enteropathy in celiac disease, a common autoimmune disorder of the small intestine. Individuals
who suffer from celiac...
A new, improved version of the catalog of 182 alleles at the six Gli loci of common wheat (T. aestivum L.) shown in electrophoregrams of 128 standard genotypes was used for analysis of 1060 cultivars and lines bred in the 20th century. The most frequent alleles in the studied germplasm occurred with frequencies of 18%–40%, with 30 unique alleles, o...
Future progress on the creation of wheat cultivars with high grain zinc (Zn) and iron (Fe) mineral density will depend on both the availability of suitable donor germplasm and the identification of genes or quantitative trait loci contributing to increase the accumulation of mineral elements in the wheat kernels. Multi-environment field trials were...
Genetically diverse wheat samples, twenty-seven Triticum aestivum L. varieties, grown in two environments (Portugal and Spain) were analyzed for their allelic composition in high-molecular-weight glutenin subunits (HMW-GS), low-molecular-weight glutenin subunits (LMW-GS) and puroindolines, as well as their protein content, hardness, sodium dodecyl...
Association mapping was performed for 18 agromorphological and grain quality traits in a set of 183 Spanish landraces, including subspecies durum, turgidum and dicoccon, genotyped
with 749 DArT (Diversity Array Technology) markers. Large genetic and phenotypic
variability was detected, being the level of diversity among the chromosomes and genomes...
Description of the testing environments.
Meteorological data refer from November to June.
(DOCX)
Summary table of MTAs indicating those monomorphic in some subspecies and those found in previous reports.
(XLSX)
Overview of the LD parameter r2 of the intrachromosomal pairs in the whole collection.
The scatterplots show the distributions of the LD parameter r2 according to the genetic distance. The horizontal line indicates the 95% percentile of the distribution of the unlinked r2, which gives the critical value of r2.
(TIF)
Sum of squares of the ANOVA for the quantitative agromorphological and the grain quality traits for 183 landraces of the subsp. durum, turgidum and dicoccon evaluated in four environments.
(DOCX)
Pearson’s correlation coefficients between environments for the quantitative agromorphological and grain quality traits.
(DOCX)
Significant correlation coefficients (P<0.05) between the quantitative agromorphological and grain quality traits within each subspecies and environment.
(DOCX)
DArT markers monomorphic in the subsp. durum, turgidum and dicoccon, chromosome location and putative function.
(XLSX)
Durum wheat consensus map from Marone et al. (2012).
MTAs identified in the present study are marked in bold.
(XLSX)
Protein engineering of gluten, the exogenous effector in celiac disease, seeking its detoxification by
selective chemical modification of toxic epitopes is a very attractive strategy and promising technology
when compared to pharmacological treatment or genetic engineering of wheat. Here we present a simple
and efficient chemo-enzymatic methodology...
Thermal imaging has been used to evaluate the response to drought and warm temperatures in a collection of Brachypodium distachyon lines adapted to varied environmental conditions. Thermographic records were able to separate lines from contrasting rainfall regimes. Genotypes from dryer environments showed warmer leaves under water deficit, which su...
tCover crop selection should be oriented to the achievement of specific agrosystem benefits. The covercrop, catch crop, green manure and fodder uses were identified as possible targets for selection. Theobjective was to apply multi-criteria decision analysis to evaluate different species (Hordeum vulgareL., Secale cereale L., ×Triticosecale Whim, S...
Cover crop selection should be oriented to the achievement of specific agrosystem benefits. The cover crop, catch crop, green manure and fodder uses were identified as possible targets for selection. The objective was to apply multi-criteria decision analysis to evaluate different species (Hordeum vulgare L., Secale cereale L., ×Triticosecale Whim,...
In a scenario of climate change and rapidly rising urban populations demanding processed foods, it is necessary to develop new wheat cultivars combining high yield potential, disease resistance, and stability for yield and processing quality, even under heat or drought stress conditions. Allelic variation for gluten proteins (glutenin subunits and...
Bread wheat quality constitutes a key trait for the demands of the baking industry as well as the broad consumer preferences. The role of the low molecular weight glutenin subunits (LMW-GS) with regard to bread quality is so far not well understood owing to their genetic complexity and to the use of different nomenclatures and standards for the LMW...
The annual grass Brachypodium distachyon has been recently recognized as the model plant for functional genomics of temperate grasses, including cereals of economic relevance like wheat and barley. Sixty-two lines of B. distachyon were assessed for response to drought stress and heat tolerance. All these lines, except the reference genotype BD21, d...
Spanish wheat (Triticum spp.) landraces have a
considerable polymorphism, containing many
unique alleles, relative to other collections.
The existence of a core collection is a favored
approach for breeders to efficiently explore
novel variation and enhance the use of
germplasm. In this study, the Spanish durum
wheat (Triticum turgidum L.) core col...
Glutens, the storage proteins in wheat grains, are a major source of protein in human nutrition. The protein composition of wheat has therefore been an important focus of cereal research. Proteomic tools have been used to describe the genetic diversity of wheat germplasms from different origins at the level of polymorphisms in alleles encoding glut...
Durum wheat (Triticum turgidum ssp. durum), one of the most important cultivated species in North-Africa, is mainly used for pasta and, to a lesser extent, as bread and couscous for human consumption. A lot of breeding programs are concentrated mainly on the dough quality. Quality value is determined by protein content, glutenin and gliadin allelic...
Knowledge of the genetic structure of germplasm
collections is crucial for conservation and
efficient use of genetic resources. This study
assessed the diversity and genetic structure
of a collection of landraces of Spanish durum
wheat (Triticum turgidum L.) using several
marker systems and correlated the diversity
and agromorphological traits with...
With 1 figure and 1 table
The correct assignment of high molecular weight glutenin subunit variants is a key task in wheat breeding for quality. However, traditional analysis by protein electrophoresis is sometimes difficult and not very precise. This work describes a novel DNA marker for accurate discrimination between the Glu-B1 locus subunits Bx...
Knowledge of the genetic structure of germplasm collections is crucial for conservation and efficient use of genetic resources. This study assessed the diversity and genetic structure of a collection of landraces of Spanish durum wheat (Triticum turgidum L.) using several marker systems and correlated the diversity and agromorphological traits with...
Brachypodium distachyon (L.) P. Beauv. (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20, and 30 chromosomes) has long been regarded as a case of autopolyploid series with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium spe...
Reducing duplication in ex-situ collections is complicated and requires good quality genetic markers. This study was conducted to assess the value of endosperm proteins and SSRs for validation of potential duplicates and monitoring intra-accession variability. Fifty durum wheat (Triticum turgidum ssp. durum) accessions grouped in 23 potential dupli...
Using C-banding, homologous and homoeologous meiotic pairing between wheat (AB), rye (R), and Aegilops caudata (C) genomes were estimated at meiotic metaphase I in trigeneric hybrids (AABBRC; 2n = 6x = 42). In all hybrids, the C-genome chromosomes pair homoeologously only with chromosomes of the A genome, but not with chromosomes of the B genome or...
The SDS-PAGE pattern of reduced and alkylated glutenins from the bread wheat cultivar Prinqual presents a subunit (named d4) in the mobility zone of the omega -gliadins that only appears under reduced conditions. This subunit was isolated and characterised at the biochemical and genetic levels. Subunit d4 was shown to form disulphide aggregates wit...
The allelic variation of high molecular weight glutenins as principal determinants of bread quality has been analyzed in 165 Spanish wheat (Triticum aestivum ssp. vulgare L.) landraces provided by the Plant Genetic Resources Centre. The identification by standard electrophoresis techniques has been supported by a new PCR screening method, allowing...
Khorasan wheat, Triticum turgidum ssp. turanicum (Jakubz), is an ancient cultivated wheat possessing several characteristics that may be useful for durum wheat improvement. In this paper, the allelic variation of prolamins, principal determinants of pasta quality, has been studied in 77 accessions from the USDA‐ARS germplasm collection. Some prolam...
Bread wheat (Triticum aestivum L.) quality is a key trait for baking industry exigencies and broad consumer preferences. The main goal of this study was to undertake quantitative trait loci (QTL) analyses for bread wheat quality in a set of 79 recombinant inbred lines (RILs) derived from a soft×hard bread wheat cross. Field trials were conducted ov...
The effects of environment and the high molecular weight glutenins on some quality properties ( sedimentation volume, % protein content, and starch pasting viscosity) of bread wheat mutant waxy lines were evaluated. Thirty-eight 100% amylose-free F(2) derived F(6) and F(7) lines were used. The results indicated that the environment did not influenc...
A subset of durum wheat Spanish landraces, previously evaluated for yield at low and high nitrogen (N) levels, was analysed for quality, protein content (P) and sodium dodecyl sulphate sedimentation (SDSS) test. The evaluation was carried out at the two N rates and in two years. The influence of prolamin alleles at the Glu-1, Glu-3, Glu-B2 and Gli-...
Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI ( Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, differen...
Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different...
The allelic variation at seven prolamin loci involved in quality has been studied in a set of durum wheat landraces from all the Spanish regions where this crop has been traditionally cultivated. The genetic variability was higher than that found in other germplasm collections. All the loci, except Glu-B2, displayed a genetic variability higher tha...
Aegilops geniculata Roth is an annual grass relative to cultivated wheats and is widely distributed in North Africa. In order to understand the
diversity of this species, 14 populations collected in different bioclimatic areas in northern Algeria were analyzed using
morphological and biochemical characters. Principal component analyses (PCA) based...
Gliadin alleles were identified in 100 common wheat cultivars registered and/or grown in Spain during the last 40 years. A very high level of genetic polymorphism was found: in total, 103 allelic variants including one null-allele were found at the six major Gli loci in the Spanish wheats studied. An average genetic diversity for these six loci was...
A collection of 547 durum wheats (103 cultivars and 444 landraces) from Spain was analysed for waxy protein composition. The electrophoretic patterns showed low polymorphism. At the Wx-A1 locus, 99.8% of the wheats had the Wx-Ala allele and only one had the null Wx-Alb allele. The Wx-Bl locus was more polymorphic and four different alleles were det...
A collection of 89 accessions of diploid species of wheat was analysed for waxy protein in the grain: 39 accessions of Einkorn wheats, 41 accessions of Sitopsis section wheat and nine accessions of Triticum tauschii. The electrophoretic patterns showed low polymorphism. In each group of wheat, a single and different allele was detected. In accessio...
The high molecular weight (HMW) and B-zone low molecular weight (B-LMW) glutenin subunit composition of 45 Algerian durum wheat (Triticum turgidum L. var. durum) landraces and old cultivars were examined by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Nine accessions were heterogeneous and presented two or three genotypes....
We have used in situ hybridization combining genomic and repeated DNA fluorescent probes to determine the karyotype composition of two bread wheat introgression lines: H-93-33, which carries the gene H27 for resistance to the Hessian fly M. destructor (Delibes et al. 1997); and H-93-8, carrying the gene Cre2 which confers resistance to the cereal c...
Se conoce como encamado el proceso por el que los tallos de las plantas son desplazados de una manera permanente de su posición vertical. Puede culminar en que las plantas se queden dobladas o tumbadas sobre el suelo, y a veces puede llevar rotura de los tallos. Los tallos pueden permanecer rectos en su encamado o adoptar formas curvas. El encamado...
A core subset of Spanish durum wheat landraces was evaluated at two nitrogen levels (80 and 220 kg/ha) to identify landrace genotypes adapted to low N production. Yield differences were statistically significant between N levels and among genotypes at both levels. Fifty-one per cent of the landraces yielded significantly more at low than at high N...
This work studied the combined use of gliadins and SSRs to analyse inter- and intra-accession variability of the Spanish collection
of cultivated einkorn (Triticum monococcum L. ssp. monococcum) maintained at the CRF-INIA. In general, gliadin loci presented higher discrimination power than SSRs, reflecting the high
variability of the gliadins. The...
This book brings together recent, international contributions to the study of gluten proteins from leading experts in the field. Gluten proteins have gained greater importance due not only to their fundamental role in determining technological quality of wheat end products, but also to the apparently increased number of people showing different deg...
In this work gliadin proteins were used to analyse the genetic variability in a sample of the durum wheat Spanish collection
conserved at the CRF-INIA. In total 38 different alleles were identified at the loci Gli-A1, Gli-A3, Gli-B5, Gli-B1, Gli-A2 and Gli-B2. All the gliadin loci were polymorphic, possessed large genetic diversity and small and la...
An electrophoretic study of gliadin and glutenin proteins, mainly low-molecular-weight (LMW) Glutenin Subunits, was undertaken to investigate possible assoeiations between these proteins and gluten strength. Thirty-eight durum wheat cultivars having different origins and currently grown in Spam were analysed. Different electrophoretic methods were...
The objective of this paper was to study the differences between some prolamin variants coded at the loci Glu ‐3/ Gli ‐1, Glu ‐1 and Gli‐A 2 and their relative roles in durum‐wheat quality. F 3 lines from four durum wheat crosses ( ‘Abadia’בMexicali’. ‘Oscar’בArdente’ , ‘Oscar × Mexicali’ and ‘Alaga’בC. of Balazote’) were analysed for gliadin an...
By electrophoretic analysis of F2 progenies from crosses among the hexaploid wheat varieties ‘Cajeme 71’, ‘Ablaca’, ‘Anza’ and ‘Pané 247’, two new prolamin loci Gli-D4 and Gli-D5, were mapped on the short arm of chromosome ID. The Gli-D4 locus controls gliadins of type γ and is situated on the short arm of chromosome ID between the centromere and t...
A collection of 130 cultivars of bread wheat, 332 landraces of bread wheat and 144 spelt wheats was analysed for waxy proteins in the grain. The electrophoretic patterns showed very low polymorphism and most of the hexaploid wheats had the Wx-Ala, Wx-D1a and Wx-B1 alleles of ‘Chinese Spring’. Two alleles were detected at Wx-A1 (Wx-A1a, and Wx-A1b (...
Two bread wheat cultivars, ‘Ariana 8’ and ‘Cajeme 71’, and 129 F2, grains from the cross between them were analysed for gliadin composition. Two monodimensional (A-PAGE and SDS-PAGE) and two different two-dimensional (SDS-PAGE x SDS-PAGE and A-PAGE x SDS-PAGE) electrophoretic methods were used. Parents differed at the Gli-Al locus, detected by A-PA...
A study was made of the effects of the Gli-B3/Glu-B2 encoded prolamins on durum-wheat quality. Twenty-six F3 lines from the durum wheat cross ‘Abadia’בMexicali 75’ were analysed electrophoretically for prolamin composition and for the following quality parameters: SDS sedimentation value, mixing properties, and percentage grain protein and percent...
The F4 progenies of four durum wheat crosses were used to determine the effects of different prolamin alleles on quality properties evaluated by the SDS sedimentation, mixograph, micro-alveograph and vitreousness tests and by protein content. Allelic compositions of the gliadins (Gli-B1 and Gli-2 loci) and the glutenins (Glu-1, Glu-3 and Glu-B2 loc...
The B low Mr subunits of glutenin of the F2 generation from three durum wheat crosses were analysed. Three new alleles were found at three different loci: Glu-A3i coding for 5+20 subunits, Glu-B2c coding for subunit 12* and Glu-B3l coding for 1+3+13*+16 subunits. The genetic distances between Glu-A3-Gli-A1, Glu-B2-Gli-B1, Glu-B3-Glu-B2 and Glu-B3-G...
This book brings together recent, international contributions to the study of gluten proteins from leading experts in the field. Gluten proteins have gained greater importance due not only to their fundamental role in determining technological quality of wheat end products, but also to the apparently increased number of people showing different deg...
This paper reports the waxy proteins and apparent amylose contents of the tetraploid species Triticum dicoccum, Triticum polonicum and Triticum durum. Three waxy proteins were found in the three species; two showed the same electrophoretic mobility as the alleles Wx-A1a and Wx-B1a of the hexaploid variety ‘Chinese Spring’, while the third showed th...
Gliadin analysis has shown that Spanish common wheat germplasm is highly polymorphic and rather unique. In total, 81 different alleles, 70 of them catalogued, were identified in the 52 Spanish landraces studied, and 25 new gliadin alleles, not present in germplasm from other countries, were found. Eleven alleles were identified at the Gli-A1 locus,...
The high molecular weight glutenin subunit (HMW-GS) composition of acollection of 107 Argentinean bread wheat cultivars was analysed bysodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).Allelic variation at the Glu-1 loci was identified and its frequencycalculated. Eleven alleles were detected, three encoded at the Glu-A1locus, s...
Fifty nine Spanish landraces of common wheat belonging to differentagrotypes were evaluated with four morphological spike characters and 11 seedprotein loci in order to study the level of agreement between the agrotypeclassification and the morphological and biochemical variation of the wheats. Asa result of the work 22 new gliadin alleles were fou...
A collection of 136 accessions of Aegilops umbellulata (39), Ae. comosa (75) and Ae. markgrafii (22) was analysed for high-molecular-weight (HMW) glutenin subunits composition. The homogeneity of the accessions was studied and 55.1% of the collection was homogeneous for HMW glutenin subunits (29 Ae. umbellulata, 33 Ae. comosa and 14 Ae. markgrafii)...
The rheological properties of flours from five different lines of transgenic wheat that either express or over-express subunits 1Ax1 and 1Dx5 were analyzed by mixograph assays and SDS sedimentation tests. In one case, the over-expression of subunit 1Dx5 resulted in a ca. 2-fold increase in mixing time, associated with a significant improvement in d...
1BL.1RS wheat (Triticum aestivum L.)-rye (Secale cereale L.) translocation has been widely used in wheat breeding programs all over the world. Serious defects in bread quality have been associated with the presence of the translocation. The aim of this study was to evaluate the cumulative and interaction effects on strength and mixing properties of...
Cereal Chem. 78(1):59–63 The progenies of four intervarietal durum wheat crosses were used to determine the effects of glutenin variants coded at Glu-1 and Glu-3 loci on durum wheat quality properties. The F 2 lines were analyzed for high molecular weight (HMW) and low molecular weight (LMW) glutenin composition by electrophoresis. Whole grain deri...
The allelic variation in the high molecular weight (HMW) glutenin subunits encoded by the Glu-D1 locus has an important effect on bread-making quality. Moreover, wheats which possess the pair 5+10 have higher gluten strength than those with the pair 2+12. Some work has been done in order to elucidate which type of subunit is more important. The pre...
The correct evaluation of the breadmaking quality of 1BL/1RS wheat-rye translocation lines in breeding programs requires the use of tests which are most suitable to the peculiarities of these materials. The SDS-sedimentation test, the Pelshenke index, the 10-gram mixograph and the Chopin microalveograph have been jointly used in four sets of F3-F4...
The F2 progenies, from four crosses between landraces and commercial Portuguese durum wheat cultivars, were analysed by electrophoresis for storage protein composition and genetic segregation. The chromosomal location of genes was determined for gliadin and glutenin allelic variations at the Gli-A1, Glu-A3 and Glu-A1 on 1A; Gli-B1, Glu-B3, Gli-B3,...
Bread, pasta, noodles ... some of the many ways in which humans consume wheat after processing has taken place. The gluten proteins of wheat grain, which determine the processing properties of wheat flour, have been the subject of intensive study for many years. The structures, genetics and functional properties of this unique group of proteins are...