Jose Antonio Rodriguez-de la Garza

Autonomous University of Coahuila | UAdeC · Departamento de Biotecnología

The biotechnological importance at industrial levels is increasing everyday. By combining the ability of residual materials or responsible exploitation of biomass with biotechnological means, the overall process becomes safer and envirnomentally friendly. There is an extensive array of compounds of natural origin, which have been attributed with se...

Batch tests were carried out to investigate the production of H2 considering the effects of: substrate concentration in a range of 3–25 g-COD/L; Initial pH: from 4 to 7 and 11 and temperatures of: 20, 35, 45 and 55 °C. The optimal substrate was 25 g-COD/L, with a reduction of COD of 73% and a yield of H2 of 5.95 mM/gCOD; and the optimal initial pH...

Bovine ruminal fluid (BRF) bioaugmented with Clostridium acetobutylicum (Clac) was assessed for hydrolyzing cellulose and produce biohydrogen (BioH2) simultaneously from pretreated corncob in a single step, without the use of external hydrolytic biocatalysts. The corncob was pretreated using three thermochemical methods: H2SO4 2%, 160 °C; NaOH 2%,...

There is ongoing research related to the production of molecular hydrogen today and algae have proven to be good biological models for producing several compounds of interest. We analyzed how genetic variations in hydrogenase genes ( hyd ) can affect the production of molecular hydrogen in the algae Chlorella vulgaris and Scenedesmus obliquus . Thr...

A comparison between microwave and ultrasound irradiations in the agave pretreatment using dilute sulfuric acid as catalyst was assessed for the first time. Pretreatments were performed using a Taguchi Orthogonal Array L9 (34) to improve the hemicellulose removal and the agave digestibility. The results showed that under optimal conditions, the hem...

Castor plant is used commonly for oil extraction and biodiesel synthesis. However, the residues during pruning are not being used effectively. These residues have the potential to be used as feedstock to produce bioethanol and other by-products. The present work assessed the eco-friendly autohydrolysis pretreatment of castor plant pruning residues...

The present work was to study the fermentation and the hydraulic retention times effect using surface response methodology for hydrogen production using a synthetic media as sustrate. The reactor used 60 spheres with anaerobic biofilms developed in fiber ixtle for immobilization of anaerobic sludge and was operated at mesophilic temperatures and in...

The biological production of H2 represents a renewable and eco-friendly energy alternative compared to fossil fuels. However, its production from lignocellulose involves the use of expensive enzymatic complexes. In the present work, the production of H2 from pretreated agave biomass was evaluated by means of a Consolidated Bioprocess (CBP). This st...

The present work focused in assessing the hydrogen production from pretreated wastes of the paper industry (PIW) by simultaneous saccharification and fermentation (SSF) using anaerobic biofilms developed in natural fibers (ixtle) at different conditions. Anaerobic sludge from brewery wastewater treatment was used for biofilms and they were develope...

The aimed of this work was to determine the optimal conditions for oil extraction of Ricinus communis L. seed by means of ultrasound. The effect of 3 factors over oil yield extraction were evaluated: 1) seed pretreatment 2) ultrasound time and 3) solvent mixture ratio compared to conventional Soxhlet extraction. The optimal conditions for oil extra...

In this work, three Clostridium strains were tested for butanol production from Agave lechuguilla hydrolysates to select one for co-culturing. The agave hydrolysates medium was supplemented with nutrients and reducing agents to promote anaerobiosis. Clostridium acetobutylicum ATCC 824 had the highest butanol production (6.04 g/L) and was selected f...

El objetivo del presente trabajo fue evaluar a diferentes tiempos de retenci´on hidr´aulica (TRH) la remoci´on de materia org´anica y el voltaje generado durante el proceso de tratamiento de agua residual municipal, utilizando una celda de combustible microbiana a escala laboratorio (12 L) en modo secuencial anaerobio-aerobio (´anodo/c´atodo) emple...

Different strategies were assessed for the production of ethanol from Agave lechuguilla that was pretreated by autohydrolysis. Separate hydrolysis and fermentation (SHF) was compared against simultaneous processes including simultaneous saccharification and fermentation (SSF) and prehydrolysis and simultaneous saccharification and fermentation (PSS...

Different strategies were assessed for the production of ethanol from Agave lechuguilla that was pretreated by autohydrolysis. Separate hydrolysis and fermentation (SHF) was compared against simultaneous processes including simultaneous saccharification and fermentation (SSF) and prehydrolysis and simultaneous saccharification and fermentation (PSS...

Agave lechuguilla cogollos (heart or pulpy central stem with attached leaf bases) were used as feedstock for production of second generation bioethanol following a scheme based on pretreatment by autohydrolysis, enzymatic saccharification and fermentation of hydrolysates. Autohydrolysis was carried out in a Parr reactor at different severity factor...

Se diseñó un sistema de tratamiento de aguas residuales domésticas empleando una cepa microalgal de Chlorella vulgaris en un biorreactor airlift, con la finalidad de tratar de remover algunos de los parámetros básicos que componen el agua residual entre los cuales se encuentran: Nitrógeno (N), Fosforo (P), Demanda Química de Oxígeno (DQO). Debido a...

The aim of the present work was to assess the autohydrolysis pretreatment of Agave tequilana bagasse for ethanol production. The pretreatment was conducted using a one-liter high pressure Parr reactor under different severity factors (SF) at a 1:6 w/v ratio (solid:liquid) and 200 rpm. The solids obtained under the selected autohydrolysis conditions...

Polyurethane (PU) foams were coated with graphite, and pyrrole monomer was subsequently polymerized onto its surface by chemical oxidization to obtain nanostructured polyurethane/graphite/polypyrrole (PU/Graph/PPy) composites, which were used for anaerobic microorganisms grown and tested as anodes in microbial fuel cells (MFC) using municipal waste...

Lignocellulosic wastes from agriculture are potentially useful as a low cost feedstock for production of bioethanol. During pretreatment of lignocellulosic materials, a wide range of inhibitory compounds to microorganisms are formed or released. The present work focused on production of bioethanol by Z. mobilis NRRL B-806 in different culture media...

Ethanol production by Zymomonas mobilis NRRL-806 was assessed using different enzymatic hydrolysates from pretreated Eucalyptus globulus saw dust pulp. Results showed that when enzymatic hydrolysate containing 79.5 g L-1 of glucose and 8.81 g L-1 of acetic acid, a maximum ethanol yield 92% and a productivity of 1.16 g L-1 h-1 were obtained, with a...

In this work, a composite from α-cellulose coated with conducting polypyrrole by in situ polymerization using potassium persulfate as oxidant was obtained. The composite was characterized by fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry, UV/Vis spectroscopy, and scanning electron microscopy (SEM) analysis showed homogeneous coa...

The present study focused on the use of a composite electrode in the anodic compartment to increase energy output and reduce the cost of Microbial Fuel Cell (MFC). In order to transfer electrons by conduction by electrogenic microorganisms to the electrode this have to be in direct contact with the electrode, being this a limitation to MFC due to l...

Thermomyces lanuginosuslipase wasimmobilized in PVAalginate beads, obtaining immobilization % in the range of 94.4-98.4% using PVA concentrations ranging from 11% to 12.5%, and with crosslinking times of 45 and 60 min using boric acid. Initial reaction rate was determined in free and immobilized state by hydrolysis of p-nitrophenol palmitate. Opera...

Fermentative hydrogen production was studied in packed bed batch reactors to assess the influence of environmental factors over yield hydrogen production from dairy wastewater. Dried stems of Opuntia imbricata were used as substratum adding a pretreated mixed culture for biofilm formation. Experimental results showed that, yield hydrogen production...

The present research focused on fermentative production of hydrogen by anaerobic biofilms from a pretreated mixed microflora in a continuous fixed bed-reactor. Microbial mixed culture was thermally (100°C, by 30 min) and acidic (pH 3.0, 24 h) pretreated. Metagenomic analysis of the pretreated microbial cultured was conducted. Microbial cultured com...

Kinetics of the production and consumption of volatile fatty acids (VFAs) such as acetic, propionic, butyric acids and alcohols during anaerobic digestion of model wastewater was studied under different concentrations of sorbitol (g/l) as carbon source, pH, temperature, and granular sludge (GS). Methane production was measured. Higher COD removal a...

Kinetics of the production and consumption of volatile fatty acids (VFA) such as acetic-, propionic-, butyric- acid and alcohols during anaerobic digestion of model wastewater was studied under different concentrations of sorbitol (g/l) as carbon source, pH, temperature and granular sludge (GS). Methane production was measured. Higher COD removal a...