Jose A Herrera-Cervera

Jose A Herrera-Cervera
University of Granada | UGR · Department of Plant Physiology

PhD

About

39
Publications
5,180
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Introduction
Jose A Herrera-Cervera currently works at the Department of Plant Physiology, University of Granada. Their most recent publication is 'Polyamines contribute to salinity tolerance in the symbiosis Medicago truncatula-Sinorhizobium meliloti by preventing oxidative damage'.
Additional affiliations
February 2003 - present
University of Granada
Position
  • Professor (Full)

Publications

Publications (39)
Article
Current demand of consumers for healthy and sustainable food products has led the industry to search for different sources of plant protein isolates and concentrates. Legumes represent an excellent nonanimal protein source with high‐protein content. Legume species are distributed in a wide range of ecological conditions, including regions with drou...
Article
Full-text available
Polyamines (PAs) are ubiquitous low molecular weight, molecules found in all living cells with participation in a variety of processes. The establishment of symbiotic interactions between soil nitrogen fixing bacteria (rhizobia) and leguminous plants, as well as the formation of the root nodules where the rhizobia are hosted, depends on the product...
Chapter
Polyamines (PAs) are hormonal compounds and growth regulators, with low molecular weight, aliphatic nature, and polycationic character at physiological pH, present in different types of organisms and particularly in plants, where they are involved in the regulation of various physiological processes related to growth and development as well as in r...
Chapter
Alternative respiration has been suggested to play important roles under salinity conferring plant tolerance. On the one hand, it has been proposed as an antioxidant mechanism that may reduce oxidative damage by avoiding the generation of reactive oxygen species (ROS) that may occur as a consequence of an overreduction of the ubiquinone (UQ) pool i...
Article
Legumes have the capacity to fix nitrogen in symbiosis with soil bacteria known as rhizobia by the formation of root nodules. However, nitrogen fixation is highly sensitive to soil salinity with a concomitant reduction of the plant yield and soil fertilization. Polycationic aliphatic amines known as polyamines (PAs) have been shown to be involved i...
Article
Full-text available
Polyamines (PAs) are small nitrogen polycationic compounds involved in the response to salinity as well as in biotic interactions. Legumes are salt sensitive species characterised by their ability to establish symbiotic interactions with nitrogen fixing bacteria known as rhizobia that colonize the roots inducing the formation of nodules where the n...
Article
Polyamines (PAs) such as spermidine (Spd) and spermine (Spm) are small ubiquitous polycationic compounds that contribute to plant adaptation to salt stress. The positive effect of PAs has been associated to a cross-talk with other anti-stress hormones such as brassinoesteroids (BRs), also involved in anti-stress responses in plants. In this work we...
Article
Brassinosteroids (BRs) are steroid plant hormones that have been shown to be involved in the response to salt stress in cross-talk with other plant growth regulators such as polyamines (PAs). In addition, BRs are involved in the regulation of the nodulation in the rhizobium-legume symbiosis through the alteration of the PAs content in leaves. In th...
Article
Full-text available
In this study, we examine how indeterminate and determined nitrogen-fixing root nodules of model legumes Lotus japonicus and Medicago truncatula adapt their non-structural carbohydrate pool during salt stress, with particular emphasis on trehalose, a compatible solute abundant in nodules of some legumes. M. truncatula and L. japonicus plants were i...
Article
This study compared growth, nodulation, nitrogen fixation, and nodular enzyme activities in response to salinity in some common bean-rhizobia symbiotic combinations. Seeds of Paulista and Efequince, two varieties of the common bean (Phaseolus vulgaris) were germinated and seedlings were transferred to pots containing vermiculite inoculated with the...
Chapter
Full-text available
Resumen Los estreses abióticos afectan negativamente al rendimiento de las leguminosas principalmente en suelos pobres en nitrógeno. Esto se debe a que la simbiosis Rhizobium-leguminosa y la fijación simbiótica de nitrógeno son altamente sensibles a dichos estreses. Es posible utilizar diferentes estrategias para dotar a las leguminosas de una mayo...
Conference Paper
Full-text available
The leguminous plants form nodules through their symbiotic association with the rhizobia. These organs provide the necessary nitrogen for the plants growth and the development and contribute to the improvement of nitrogen level in the soil. Thus this symbiosis could replace the chemical fertilizer applications, which are expensive and polluting the...
Conference Paper
Full-text available
The objectives of this study were to assess the performance of common bean-rhizobia symbiosis for biological nitrogen fixation (BNF) under phosphorus (P) deficiency and to determine some physiological and molecular parameters involved in the tolerance mechanisms. A common bean germoplasm of five varieties and four recombined lines is considered in...
Article
Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranoside), a non-reducing disaccharide, has been found in a wide variety of organisms playing an important role as an abiotic stress protectant. Plants may come into contact with trehalose from exogenous sources, such as in plant-rhizobia symbiosis in which the rhizobia have the capacity to produ...
Conference Paper
Full-text available
A common bean (Phaseolus vulgaris) germplasm, consisting of six varieties and four lines, was tested through field trials, in five farmer's fields with different soil phosphorus levels and two experimental stations at the Haouz area. The objective of field trials was to assess the behavior of common bean genotype/rhizobia strain combinations and to...
Article
Lotus japonicus and Medicago truncatula model legumes, which form determined and indeterminate nodules, respectively, provide a convenient system to study plant-Rhizobium interaction and to establish differences between the two types of nodules under salt stress conditions. We examined the effects of 25 and 50mM NaCl doses on growth and nitrogen fi...
Article
The effect of NaCl stress (50 mM) and validamycin A treatment (30 μM) on growth and nitrogen fixation of Lotus japonicus was investigated in plants cultured under symbiotic and hydroponics conditions for teen weeks (flowering stage). Validamycin A was used as a potent trehalase inhibitor, and was able to produce a five-fold increase in the level of...
Article
Full-text available
Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain (N265TraA), we demonstrated nic binding and cleavage activity in vitro and thus characterized for the first time the nick site (nic) of a plasmid in the family Rhizobiaceae. We studied...
Article
The genetic relationships among bacteria nodulating Phaseolus vulgaris in a soil of Granada, Spain, were investigated by multilocus enzyme electrophoresis, restriction fragment length polymorphism and partial sequencing of the 16S rRNA genes and restriction fragment length polymorphisms of symbiotic genes. Multilocus enzyme electrophoresis analysis...
Article
Rhizobium meliloti recA strains have been reported as safe hosts for deliberate release experiments due to their reduced survival in soil microcosms. Using R. meliloti integration vectors, two GUS-positive RecA+ and RecA− isogenic derivatives of R. meliloti strain GR4 were constructed. In contrast to previously reported RecA− strains, the RecA− str...
Article
Full-text available
An analysis of the conjugative transfer of pRetCFN42d, the symbiotic plasmid (pSym) of Rhizobium etli, has revealed a novel gene, rctA, as an essential element of a regulatory system for silencing the conjugative transfer of R. etli pSym by repressing the transcription of conjugal transfer genes in standard laboratory media. The rctA gene product l...
Article
The purification and characterization of trehalase from common bean nodules as well as the role of this enzyme on growth, nodulation nitrogen fixation by examining the effects of the trehalase inhibitor validamycin A, was studied. Validamycin A did not affect plant and nodule mass, neither root trehalase and nitrogenase activity; however this treat...
Chapter
Full-text available
Lotus japonicus has gained considerable attention because of its increasing use as a model legume. It is thus important to have an effective transformation and regeneration procedure to allow the transfer of foreign genes into Lotus for complementation experiments and the studies of regulation of nodulation and developmental processes. Agrobacteriu...
Article
A transposon tagging strategy is described, based on modified Ds elements, and aimed at tagging genes located in regions of the genome where map based cloning is virtually impossible; for example, near centromeres or in sub-telomeric regions. The launching vectors constructed have the Ds elements and the transposase gene within the same T-DNA. Two...
Article
Full-text available
An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob clones. By following this modif...
Article
Full-text available
Summary • The expression of nodA and dctA genes of Rhizobium leguminosarum bv. viciae has been studied in mutant nodules of pea (Pisum sativum L.), blocked at the following developmental stages: infection thread development inside the nodule (Itn); infection droplet differentiation (Idd); bacteroid differentiation after endocytosis (Bad); and nodul...
Chapter
The direct consequences of this phenomenon are loss of inoculation efficacy, as the introduced inoculant strain can be outcompeted by new emerging strains, which could be poor nitrogen-fixers but will occupy most of the nodules, providing little nitrogen to the current crop. Similar problems may arise if new legume genotypes are introduced, along w...
Chapter
Analysis of the genetic structure of soil populations provides abundant evidence indicating that Rhizobium spp. exchange DNA. For instance, among native field populations, the same symbiotic plasmid can be found in otherwise unrelated strains, and vice versa, chromosomally related strains may harbour different symbiotic plasmids (Vlassak and Vander...
Article
Full-text available
A simple approach was used to identify Rhizobium meliloti DNA regions with the ability to convert a nontransmissible vector into a mobilizable plasmid, i.e., to contain origins of conjugative transfer (oriT, mob). RecA-defective R. meliloti merodiploid populations, where each individual contained a hybrid cosmid from an R. meliloti GR4 gene library...
Article
Full-text available
Inoculation of alfalfa plants with either incompatible Rhizobium or a Rhizobium mutant blocked in Nod factor synthesis led to an accumulation of salicylic acid in roots, in contrast to plants inoculated with a wild-type (compatible) R. meliloti strain. When salicylic acid was exogenously applied prior to inoculation of alfalfa plants with either pu...
Article
Full-text available
We have examined nutritional factors influencing conjugal transfer of the two nonsymbiotic large plasmids, pRmeGR4a and pRmeGR4b, of Rhizobium meliloti GR4. To monitor transfer, each plasmid was tagged with a different antibiotic resistance marker. Transfer of plasmid pRmeGR4b was dependent upon the presence of plasmid pRmeGR4a on the same donor ce...

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