Jorge Luiz Ninow

• Universidade Federal de Santa Catarina

Nanobiocatalysts were produced via immobilization of CalB lipase on polyurethane (PU) based nanoparticles and their application on the synthesis of important industrial products was evaluated. Nanoparticles of polyurethane functionalized with poly(ethylene glycol) (PU-PEG) were synthetized through miniemulsion polymerization and the addition of cro...

This study evaluated the quality of oil extracted from yellowfin tuna (Thunnus albacares) by enzymatic hydrolysis (EHO) compared with oil extracted by traditional methods, such as the physical method of cooking and pressing after fishmeal production and the chemical solvent method. The oil extracted by EHO presented the lowest acidity (1.96% oleic...

Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the init...

The adsorption of Thermomyces lanuginosus lipase (TLL) on PEGylated polyurethane particles as support permitted the development of several strategies to improve the properties of this commercial low-cost enzyme. The supports were synthesized by miniemulsion technique using isophoronediisocyanate (IPDI) and poly(ε-caprolactone) diol (PCL530) as mono...

Neste trabalho o bagaço de maçã foi utilizado como substrato para a produção de proteína unicelular através da fermentação em estado sólido, onde o fungo filamentoso Gongronella butleri CCT4274 foi empregado no enriquecimento protéico deste resíduo. Na fermentação em estado sólido, após estudar a influência da adição de fontes de nitrogênio, umidad...

Monascus species can produce yellow, orange, and red pigments, depending on the employed cultivation conditions. They are classified as natural pigments and can be applied for coloration of meat, fishes, cheese, beer, and pates, besides their use in inks for printer and dyes for textile, cosmetic, and pharmaceutical industries. These natural pigmen...

The removal of the calyx from physalis for the commercialization of the fresh cut fruit reduces its shelf life. In this context, gelatin is a protein polymer produced on large scale at relatively low cost that may be employed as edible coating increasing the shelf life of this fruit. The objective of this study was to evaluate the effect of gelatin...

Nanotechnology is an area that has been growing over the years, being possible nowadays to find numerous materials constructed at nanoscale. In addition, many applications have been attributed to these "new" materials. In this review is presented a brief overview of nanoparticles used for the immobilization of enzymes. Considering the extensive uni...

This study aimed to determine the effect of pH on radial growth rate and pigments production from Monascus ruber CCT 3802. Fermentations were carried out in solid medium and submerged fermentations under different pH conditions and the pigments produced were submitted to scanning in spectrophotometer. Higher radial growth rate was obtained when M....

The diacylglycerols (DAG) are emulsifiers with high added value used as functional additives in food, medicine, and cosmetic industries. In glycerolysis of oils for the production of DAG, the immiscibility between the substrates (glycerol and oil phases) has to be overcome, for example, by the addition of a food grade surfactant like Tween 65. The...

Abstract In order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. As application model, it was tested the immobilization of β-D-galactosidase from Aspergillus oryzae. Chitosan particles were obtained by precipitation followed by adsorption of th...

We report the synthesis of polyurethane (PU) with polyethylene glycol (PEG) by miniemulsion polymerization as technique to produce a support for enzyme immobilization and stabilization. In order to study the influence of molar weight and concentration of PEG in the enzyme-support interaction, different PEG (400, 4000 and 6000 Da) was used during th...

Ultrasound-assisted system is promising for chemical synthesis. This study aimed the production of ascorbyl oleate catalyzed by lipase Novozym s 435 in organic solvent using ultrasound-assisted system. Different organic solvents and solvent concentration were tested at 70 °C using 1:9 M ratio ascorbic acid: oleic acid. The solubility was evaluated...

With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU–ADS), bond (using polyethyleneimine) (PU–PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU–PEI–GA) and entrapment (PU). The characterizat...

To reduce environmental problems caused by glycerine accumulation and to make the production of biodiesel more profitable, crude glycerin without treatment was used as substrate for obtaining higher value-added bioproducts. Monascus ruber is a filamentous fungus that produces pigments, particularly red ones, which are used for coloring foods (rice...

The ability of commercial immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM) to catalyze the acetylation of essential clove oil with acetic anhydride in a solvent-free system was studied, and the antimicrobial activity of the ester formed was evaluated as well. Experimental design based on two variables (eugenol to acetic anhydride mo...

This work shows new and promising experimental data of soybean oil and canola oil glycerolysis using Novozym 435 enzyme as catalyst in a solvent-free system using ultrasound bath for the emulsifier, monoglyceride (MAG), and diacylglycerol (DAG) production. The experiments were conducted in batch mode to study the influence of process variables as t...

The amino acid profile in dessert apple must and its effect on the synthesis of fusel alcohols and esters in cider were established by instrumental analysis. The amino acid profile was performed in nine apple musts. Two apple musts with high (>150 mg/L) and low (<75 mg/L) nitrogen content, and four enological yeast strains, were used in cider ferme...

With the objective to obtain immobilized Candida antarctica lipase B (CalB) with good activity and improved utilization rate, this study evaluated the influence of enzyme and crodamol concentrations and initiator type on the CalB enzyme immobilization in nanoparticles consisting of poly(methyl methacrylate) (PMMA) obtained by miniemulsion polymeriz...

The named "green chemistry" has been receiving increasing prominence due to its environmentally friendly characteristics. The use of enzymes as catalysts in processes of synthesis to replace the traditional use of chemical catalysts present as main advantage the fact of following the principles of the green chemistry. However, processes of enzymati...

The aim of this work was to evaluate apple pomace as carbon source for fungal chitosan production in an external-loop airlift bioreactor by Gongronella butleri. Apple pomace has high moisture content, insoluble carbohydrates such as cellulose, hemicellulose and lignin, reducing sugars like glucose and fructose and other sugars such as sucrose. Due...

The solid residue of apple juice extraction is the apple pomace. This processing waste represents up to 30% of the original fruit and consists of a complex mixture of peel, core, seed, calyx, stem, and soft tissue. It has high water content and is mainly composed of insoluble carbohydrates such as cellulose, hemicellulose, and lignin. Simple sugars...

The aim of this work was to present new data on the thermal stability of orange and red pigments produced by Monascus ruber CCT 3802 in submerged fermentation. The pigments were evaluated under different temperature and pH conditions in glass bottles with the temperature controlled using a thermostat bath. Samples of orange and red pigments were co...

β-d-Galactosidase from Kluyveromyces lactis was immobilized on glutaraldehyde-activated chitosan and used in a packed-bed reactor for the continuous hydrolysis of lactose and the synthesis of galactooligosaccharides (GOS). The biocatalyst was tested for its optima pH and temperature, thermal stability in the presence of substrate and products, and...

The study and use of natural pigments in food industries have increased in recent years due to the toxicity presented by artificial pigments. Monascus ruber is a filamentous fungus that produces red, orange, and yellow pigments under different growing conditions. The growth of health food market has increased in parallel with the growth in biofuels...

Antimicrobial activity of natural pigments produced by Monascus ruber CCT 3802 in submerged fermentation were studied. The pigments were screened for antimicrobial activity against three foodborne bacteria: Staphylococcus aureus, Escherichia coli and Salmonella enteritidis. The orange pigment presented antimicrobial activity against S. aureus ATCC...

Omega-3 enriched partial acylglycerols are beneficial for human health. The aim of this study was to obtain monoacylglycerols (MAG) and diacylglycerols (DAG) by means of glycerolysis of fish oil catalyzed by a lipase from Rhizomucor miehei in the presence of food grade surfactants (Tween 65, 80 or 85). Glycerolysis was successful in the reaction me...

MAGs and DAGs are widely used as emulsifiers in many industries. During glycerolysis reactions for their production, the immiscibility of the glycerol and the oil phases is an important drawback for practical purposes. For overcoming this problem, the use of a food grade surfactant can be an important alternative to improve system homogeneity, lead...

In this study, pigment production by Monascus ruber in submerged fermentation using glycerol as substrate was optimized by employing factorial design and response surface techniques. The variables evaluated were the glycerol and monosodium glutamate concentrations, having as responses pigment and biomass productions. The monosodium glutamate concen...

The effect of the support size on the properties of enzyme immobilization was investigated by using chitosan macroparticles and nanoparticles. They were prepared by precipitation and ionotropic gelation, respectively, and were characterized by Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), transmission elec...

The present work aimed at determining the oxygen saturation in culture medium used in the production of pigments by Monascus ruber CCT 3802. This estimation allows the correction and the minimization of errors on the specific oxygen uptake rates determination because the conversion of oxygen partial pressure to oxygen concentration requires accurat...

Mono- (MAG) and diacylglycerols (DAG) are of nutritional interest. MAG and DAG containing eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were produced in a solvent-free system via glycerolysis of menhaden oil catalyzed by Novozym 435. The effect of the molar ratio of glycerol to oil, enzyme concentration, and reaction temperature on MAG and...

The solid residue of apple juice extraction is the apple pomace. This processing waste represents up to 30% of the original fruit and consists of a complex mixture of peel, core, seed, calyx, stem, and soft tissue. It has high water content and is mainly composed of insoluble carbohydrates such as cellulose, hemicellulose, and lignin. Simple sugars...

In light of the increasing interest in the development of functional food, several researches have focused on the production of food grade emulsifiers of nutritional interest, especially enriched in the eicosapentaenoic (EPA) and docosahexaenoic acids (DHA). The aim of this paper is to make a review of the production of monoacylglycerols (MAG) and...

This work reports experimental data and the kinetic modeling of monoacylglycerols (MAG) and diacylglycerols (DAG) production from olive oil using a commercial immobilized lipase (Novozym 435) and tert-butanol as solvent. The kinetic modeling presented is based on the ordered-sequential Bi Bi mechanism considering glycerolysis and hydrolysis/esterif...

The objectives of this work were the construction and adaptation of a vane system in a rotational rheometer; calibration of vane system using a mineral oil, and determination of rheological properties of the broth fermentation of Monascus ruber CCT 3802 at high cell density. Batch fermentation was carried out with glucose as the sole carbon source...

The main objective of this work was the isolation and screening of microorganisms with potential for producing lipases for the synthesis of fatty esters as well as evaluating the specificity of the enzymes produced, using different alcohols (methanol, ethanol, n-propanol, and butanol) and fatty acids (oleic and lauric acids) as substrates. Promisin...

Cogumelos do gênero Pleurotus representam um alimento de custo baixo, com teor elevado de proteínas, aminoácidos essenciais, proporção elevada de ácidos graxos insaturados, diversas vitaminas e minerais, além de teores baixos de gorduras, ácidos nucléicos, açúcares e calorias. Assim, o objetivo deste trabalho foi avaliar o valor nutricional de basi...

The aim of this work was to study the production of extracellular lipases by solid state fermentation in soybean meal with different supplements. Lipase production by two microorganisms, screened by their potential for lipase production, was followed in terms of hydrolytic activity at different pH values(4, 7 and 9). The supplementation of the medi...

The main objective of this work is to present new experimental information concerning the effect of relevant process parameters related to the production of diglycerides by enzymatic glycerolysis of fish oil. Reaction products can be applied as ingredients in food industry, joining the emulsifying properties of partial glycerides along with the nut...

In this work we report experimental data regarding the glycerolysis of olive oil using Novozym 435 in tert-butanol organic system aiming at the production of monoacylglycerols (MAG) and diacylglycerols (DAG). Experiments were performed in batch mode, recording the reaction kinetics and evaluating the effects of temperature, enzyme concentration, te...

Current studies about lipase production involve the use of agro-industrial residues and newly isolated microorganisms aimed at increasing economic attractiveness of the process. Based on these aspects, the main objective of this work is to perform the partial characterization of enzymatic extracts produced by a newly isolated Penicillium crustosum...

The fish farming industry, especially the marine extractive fishery, is an activity of great economic importance in many parts of Brazil. Industrial fish processing operations generate a significant amount of wastes, which are rich in proteins and long-chain fatty acids, among which the unsaturated omega-3 fatty acids are prominent. The aim of this...

The fish farming industry, especially the marine extractive fishery, is an activity of great economic importance in many parts of Brazil. Industrial fish processing operations generate a significant amount of wastes, which are rich in proteins and long-chain fatty acids, among which the unsaturated omega-3 fatty acids are prominent. The aim of this...

The aim of this research was the partial characterization of enzymatic extracts produced by a newly isolated Penicillium sp. in submerged (SmF) and solid state fermentation (SSF). The partial characterization of the crude enzymatic extract obtained by SSF and SmF systems showed optimum activity at pH 5.5 and 47 °C, and pH 7.0 and 37 °C, respectivel...

This work reports experimental data and kinetic modeling of solvent-free glycerolysis of olive oil using a commercial immobilized lipase (Novozym 435) in the presence of Triton X-100 surfactant for the production of monoacylglycerols (MAG) and diacylglycerols (DAG). The experiments were performed in batch mode evaluating the effects of temperature...

O tratamento biológico do bagaço de maçã pelo fungo Gongronella butleri, através do processo de fermentação em estado sólido e adição deste material na alimentação de tilápias do nilo, é parte fundamental do estudo ora apresentado, razão pela qual se avaliaram a fonte de nitrogênio, a umidade inicial e a granulometria no tratamento biológico do bag...

The biological treatment of the apple pomace by Gongronella butleri in solid state fermentation and the addition of this material in fish feeding (Nile tilapia) was the fundamental part of this study. The source of nitrogen, initial moisture and granulometry in the biological treatment were appraised. The best conditions being obtained with urea, i...

This work investigates the continuous production of fatty acid ethyl esters from soybean oil in compressed fluids, namely carbon dioxide, propane and n-butane, using immobilized Novozym 435 as catalyst. The experiments were performed in a packed-bed bioreactor evaluating the effects of temperature in the range of 30-70 degrees C, from 50 to 150 bar...

Structured triglycerides (STs) containing both mediumchain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) in the same molecule offer nutritional and therapeutic benefits. The aim of this work was to establish the incorporation of MCFA into fish oil triglycerides (TAGs), while maintaining substantial levels of docosahexaenoic and eicosape...

This work aimed to assess the effect of some variables on the lipase extraction from the fermented medium in order to establish the experimental conditions that maximize the yield of the enzyme obtained from solid-state fermentation of soybean meal and a newly isolated strain of Penicillium sp. The experimental design technique was used to investig...

The main objective of this work was the optimization of the lipase production by a newly isolated Penicillium sp. in supplemented soybean meal, considering a fixed C/N ratio (6.11). The kinetic behavior of lipase production was also evaluated, quantifying activity at different pH. The production of the enzyme was maximum using a substrate particle...

Lipases are enzymes that can be secreted by several microorganisms, making interesting the biodiversity exploration for searching new microorganisms able to produce these enzymes. Many agro-industrial residues can be used as potential substrates for production of enzymes. The main objective of this work was the isolation and screening of microorgan...

In this work, we propose the reuse of apple pomace as a substrate for fungal chitosan production by liquid cultivation of Gongronella butleri CCT4274. Different concentrations of reducing sugars and sodium nitrate were added to the aqueous extract of apple pomace and the best result was obtained with 40 g/L of reducing sugars and 2.5 g/L of sodium...

This work investigates the production of fatty acid ethyl esters (FAEE) from soybean oil in compressed propane using Novozym 435 as catalyst. The experiments were performed in a batch reactor following the kinetics of the enzyme-catalyzed reaction in the temperature range of 35–65 °C, from 50 to 150 bar, enzyme concentration of 1–20 wt%, oil to eth...

Pleurotus ostreatus, worldwide known as oyster mushroom, was cultivated in banana straw using inocula produced by two different processes - liquid inoculum and the traditionally used solid inoculum. Different ratios (5, 10, 15, and 20%) were tested. Biological efficiency, yield, productivity, organic matter loss, and moisture of fruiting bodies as...

The purpose of this paper is to investigate the protein enrichment of apple pomace by Gongronella butleri through solid-state cultivation and addition of this material as feed for tilapia fry (Oreochromis niloticus). Factorial experimental design was used for the assessment of culture conditions to determine the effects of the source of nitrogen, i...

Apple pomace is the processing waste generated after apple juice manufacturing and represents up to 30% of the original fruit. This solid residue consists of a complex mixture of peel, core, seed, calyx, stem, and soft tissue. This residual material is a poor animal feed supplement because of its extremely low protein content and high amount of sug...

Pleurotus species are recognized for producing beta-glucans with important medicinal properties as a constituent of the cellular wall of the fruiting body or of the mycelium. The aims of this work were to select a culture medium that maximized the production of biomass and polysaccharides produced by Pleurotus ostreatus DSM 1833 and to evaluate the...

Apple processing waste was used as a solid substrate for Rhizopus oligosporus growth. The addition of nitrogen sources and its influence upon fungal protein production were investigated. Buffer solutions were tested for pH control in order to reach the optimum range for the fungal growth (4.0-6.5). Solid-state fermentation was carried out in labora...

Pleurotus fungi present high protein and vitamin contents and a low fat level. Based on these facts, this work aimed to evaluate some nutritional characteristics of fruit bodies of Pleurotus ostreatus and Pleurotus sajor-caju after the first harvest, using rice straw and banana straw as substrates. The banana straw substrate led to a higher protein...

The 1,3-regiospecifique lipase, Lipozyme IM, catalyzed the esterification of lauric acid and glycerol in a homogeneous system. To overcome the drawback of the insolubility of glycerol in hexane, which is extensively used in enzymatic synthesis, a mixture of n-hexane/tert-butanol (1:1, v/v) was used leading to a monophasic system. The conversion of...

Sixteen fungal strains reported in the literature as endo-inulinase producers and three bacterial strains, isolated from the dahlia rhizosphere, were analysed for endo-inulinase production. From four isolated strains (one fungus and three bacteria) the results were evaluated in terms of substrate consumption, cell growth and production of endo-inul...

The biological production of sorbitol and gluconic acid from glucose and fructose is normally performed with previously grown and concentrated Zymomonas mobilis suspensions, in reactions catalyzed by the enzymes glucose-fructose oxidoreductase (GFOR) and glucono-δ-lactonase (GL). By using the flocculent strain Z. mobilis Z1-81, mechanical methods f...

Dissertação (Mestrado)--Universidade Federal de Santa Catarina, 1980.