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Introduction
Jorge Hernández-Bello currently works at the University of Guadalajara. Jorge does research in InmunoGenetics of autoimmune and infectious diseases. Their current projects are '"Immunogenetic markers for autoimmune diseases'; "B cell differentiation and antibodies synthesis"; "Immunogenetics of systemic sclerosis" and "COVID-19 in Mexico".
Current institution
Additional affiliations
November 2017 - present
INSTITUTO DE INVESTIGACIÓN EN CIENCIAS BIOMÉDICAS
Position
- Research Associate
July 2019 - present
October 2016 - present
Education
February 2013 - January 2018
Publications
Publications (100)
The antibody response to respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a major focus of COVID-19 research due to its clinical relevance and importance in vaccine and therapeutic development. Neutralizing antibody (NAb) evaluations are useful for the determination of individual or herd immunity against SARS-CoV-2, vaccine efficacy, and hu...
This is the first study outside of clinical trials (phase I-III) evaluating the ability of the Ad5-nCoV vaccine to generate neutralizing antibodies and the factors associated with optimal or suboptimal response. In a longitudinal assay, 346 people (117 with prior COVID-19 and 229 without prior COVID-19) vaccinated with Ad5-nCoV were recruited. The...
The main expected result of a vaccine against viruses is the ability to produce neutralizing
antibodies. Currently, several vaccines against SARS-CoV-2 are being applied to prevent mortal complications, being Pfizer-BioNTech (BNT162b2) one of the first to be authorized in the USA and Mexico (11 December 2020). This study evaluated the efficacy of t...
Background: An unbalanced dietary intake of omega-6 (n-6) and omega-3 (n-3) polyunsaturated fatty acids (PUFAs) has been associated with chronic inflammation and oxidative stress, both of which contribute to the pathophysiology of obesity. Objective: We aimed to evaluate the effects of a diet with an n-6:n-3 PUFA ratio of 5:1 on body composition, b...
Background
The CKD in PLHIV is highly prevalent in Jalisco. Despite its control with ART, HIV is characterized by generating low-grade inflammation events that contribute to the development and progression of CKD. Considering the importance of hs-CRP in the context of CKD, various genetic predisposition studies have been conducted to search for var...
Background: Analyzing Y-chromosome short tandem repeats (Y-STRs) is essential in forensic genetics and population studies. The Yfiler™ Plus kit, which includes 27 Y-STR markers, enhances the discrimination power for forensic and kinship applications. However, this genetic system has not been analyzed in Mexican populations, which limits its applica...
Plaque psoriasis (PP) is a chronic immune-mediated skin disorder with a genetic basis, characterized by abnormal T-cell responses. This study investigated the role of FOXP3 gene variants rs2280883 and rs3761548 in T-cell regulation through their effects on IL-10 and TGF-β1 cytokine levels and their association with PP risk. A case-control study was...
En Jalisco, se ha encontrado una mutación en el gen PSEN1 relacionada con el Alzheimer de inicio temprano, que es común en los Altos de Jalisco. Esta mutación afecta a hombres y mujeres, causando síntomas graves como problemas para caminar y hablar, que aparecen antes de lo habitual y avanzan rápidamente, reduciendo la esperanza de vida. Además, pe...
Allergies are closely associated with sex-related hormonal variations that influence immune function, leading to distinct symptom profiles. Similar sex-based differences are observed in other immune disorders, such as autoimmune diseases. In allergies, women exhibit a higher prevalence of atopic conditions, such as allergic asthma and eczema, in co...
Studies have noted the connection between Mycobacterium avium subspecies paratuberculosis (MAP) and autoimmunity. MAP is an intracellular pathogen that infects and multiplies in macrophages. To overcome the hostile environment elicited by the macrophage, MAP secretes a battery of virulence factors to neutralize the toxic effects of the macrophage....
Las propuestas de solución de problemas son consideradas intervenciones para resolver problemas específicos en el campo de la profesión. Tradicio- nalmente, en Latinoamérica, estas intervenciones se realizan a través de procesos de Investigación-Acción Participativa (IAP), bajo el fundamento epistemológico-teórico del paradigma sociocrítico, el mat...
Este libro, aunque está dirigido a orientar principalmente a los trabajos recepcionales de los posgrados reconocidos por la Universidad de Guadalajara (UdeG), también está diseñado para ser útil a cualquier persona o programa de posgrado externo que lo necesite. Su elaboración fue coordinada por la Coordinación de Posgrado del Centro Universitario...
La tesis es un análisis académico profundo y original sobre un fenómeno de interés, que toma en cuenta el conocimiento existente en el campo correspondiente. El objetivo es contribuir al esclarecimiento del problema estudiado y hacer un aporte al estado del conocimiento existente sobre el mismo. Para ello, se requiere identificar un problema, formu...
El objetivo de esta investigación es analizar la evolución de la enfermería neonatal en México, desde sus antecedentes prehispánicos hasta su consolidación como una especialidad avanzada. Para ello, se utilizó un enfoque histórico-teórico que permitió examinar las contribuciones de figuras clave y el desarrollo de modelos científicos aplicados al c...
Se realizó un estudio transversal analítico en 1433 estudiantes de educación media superior, del Área Metropolitana de Guadalajara, Jalisco, México, en 2020. Se caracterizaron los patrones alimentarios y se evaluaron diferencias por año escolar, turno escolar y sexo. Se aplicó la Mini Encuesta de Calidad del Consumo Alimentario versión 2 mediante f...
To achieve global herd immunity, widespread vaccination is the most effective strategy. Vaccines stimulate the immune system, generating cytokines and chemokines, isotype antibodies, and neutralizing antibodies; all these molecules collectively provide a more comprehensive characterization of the immune response post-vaccination. We conducted a lon...
Introduction
COVID-19 is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a virus notable for its rapid mutation rate, which has led to the emergence of various variants such as Delta and Omicron, each with potentially different levels of transmissibility and virulence. Therefore, this study aims to compare clinical chara...
Systemic sclerosis (SSc) is characterized by a complex interplay of vascular damage, inflammation, and fibrosis, affecting the skin and internal organs. Plasminogen activator inhibitor-1 (PAI-1), a protein encoded by the SERPINE1 gene, is a potential biomarker of SSc because it is primarily involved in fibrinolysis and is associated with the severi...
During the COVID-19 pandemic, the Ad5-nCoV vaccine was applied to the Mexican population before the WHO approved it. In a transversal study, we compare the CanSino vaccine efficacy and a natural SARS-CoV-2 infection in eliciting neutralizing antibodies against the SARS-CoV-2 Delta variant in Guadalajara, Mexico. Participants between 30–60 years wer...
Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). It is estimated that more than half of new infections are transmitted by asymptomatic people; therefore, the isolation of symptomatic people is not enough to control the spread of the disease. Methods. A total of 17...
Introduction:
The macrophage migration inhibitory factor (MIF) plays a pivotal role in the development of rheumatoid arthritis (RA). Previous research indicates that MIF can trigger the expression of cytokine profiles associated with Th1, Th2, and Th17 responses in peripheral blood mononuclear cells (PBMC) from both RA patients and control subjects...
Multiple sclerosis (MS) is an autoimmune, demyelinating disorder of the central nervous system (CNS) affecting approximately 2.5 million people worldwide. The mechanisms underlying the pathogenesis of MS are still only partially elucidated. Galectins are a family of β-galactoside-binding lectins that are involved in the regulation of immune and inf...
Few studies analyze the role of B cells subpopulations in rheumatoid arthritis (RA) pathophysiology. Therefore, this study aimed to analyze the differences in B cell subpopulations and B cell activation according to disease activity, RA subtype, and absence of DMARDs therapy. These subgroups were compared with control subjects (CS). 139 subjects we...
This study aimed to analyze the dynamics, duration, and production of total and neutralizing antibodies induced by the BNT162b2 vaccine and the possible effect of gender and prior SARS-CoV-2 infection on the generation of these antibodies. Total antibodies were quantified via chemiluminescent microparticle immunoassay (CMIA), and neutralizing antib...
Introduction
The variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been classified into variants of interest (VOIs) or concern (VOCs) to prioritize global monitoring and research on variants with potential risks to public health. The SARS-CoV-2 high-rate mutation can directly impact the clinical disease progression, epid...
By January of 2023, the COVID-19 pandemic had led to a reported total of 6,700,883 deaths and 662,631,114 cases worldwide. To date, there have been no effective therapies or standardized treatment schemes for this disease; therefore, the search for effective prophylactic and therapeutic strategies is a primary goal that must be addressed. This revi...
Suicide is one of the leading causes of death worldwide. According to the World Health Organization (WHO), every year, more than 700 thousand people die from this cause. Therefore, suicide is a public health issue. The complex interaction between different factors causes suicide; however, depression is one of the most frequent factors in people who...
Population-wide vaccination is the most promising long-term COVID-19 disease management strategy. However, the protection offered by the currently available COVID-19 vaccines wanes over time, requiring boosters to be periodically given, which represents an unattainable challenge, especially if it is necessary to apply several doses per year. Theref...
Este libro es un recurso metodológico que tiene como objetivo guiar la reestructuración curricular de programas educativos de pregrado en ciencias de la salud, enfocándose en competencias profesionales integradas. Está diseñado para apoyar a los Comités Consultivos Curriculares (ccc) a fin de que desarrollen planes y programas de estudio que respon...
Systemic Sclerosis (SSc) is a chronic autoimmune disease characterized by immune disorder, microvascular damage, and fibrosis. TGFB1 gene encodes for the transforming growth factor isoform 1 (TGF-β1), one of the most important pro-fibrotic cytokines. Therefore, variants in TGFB1 and changes in its expression could be associated with the pathogenesi...
Neutralizing antibodies (NAbs) can be indicators of collective immunity, vaccine efficacy, and the longevity of the humoral response. This study aimed to compare reactogenicity and NAbs generated by three different COVID-19 vaccine platforms in individuals with and without prior COVID-19. 336 individuals vaccinated (112 with CoronaVac [inactivated...
Giovanna Georgina Ramírez-Cerón, Diana Celeste Salazar-Camarena, Claudia Azucena Palafox-Sánchez, Jorge Hernández-Bello y José Rafael Martínez-Palomar, en el capítulo 1, denominado “Factores psicosociales derivados de la pandemia por COVID-19 que afectaron el aprendizaje de estudiantes del Centro Universitario de Ciencias de la Salud de la Universi...
Citrullination is catalyzed by the peptidyl arginine deiminase 4 (PAD4) enzyme, encoded by the PADI4 gene. Increased PAD4 activity promotes the onset and progression of rheumatoid arthritis (RA). This study aimed to evaluate the association of PADI4 haplotypes with RA risk, mRNA expression, and the PAD4 activity in patients with RA from Mexico. Met...
Due to the COVID-19 pandemic, the rapid development of vaccines against SARS-CoV-2 has been promoted. BNT162b2 is a lipid-nanoparticle mRNA vaccine with 95% efficacy and is the most administered vaccine globally. Nevertheless, little is known about the cellular immune response triggered by vaccination and the immune behavior over time. Therefore, w...
Dear Editor,
We have read with great interest the report by Sahebari et al. that described the serum selenium (Se), zinc (Zn), and copper (Cu) concentrations in patients with rheumatoid arthritis (RA). The present study highlights a difference in the concentrations of these trace elements in patients with RA compared to healthy controls. In addit...
Systemic sclerosis (SSc) is characterized by chronic inflammation and fibrosis, two processes associated with transforming growth factor β (TGF-β) functions. In the present study, we investigated the expression of TGF-β isoforms in serum and the skin distribution of TGF-β and two receptors (TGF-βR1 and TGF-βR2) and their relationship with some clin...
SARS-CoV-2 variants surveillance is a worldwide task that has been approached with techniques such as Next Generation Sequencing (NGS); however, this technology is not widely available in developing countries because of the lack of equipment and limited funding in science. An option is to deploy a RT-qPCR screening test which aids in the analysis o...
Several studies have reported the benefits and safety of heterologous vaccination among different approved vaccines; however, there are no specific reports on the effects of vaccination with the Ad5-nCoV and other vaccines of the same or different technologies. In the present study, we evaluated the neutralizing antibodies percentage against SARS-C...
Background: Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease, which affects exocrine glands. T cell activation is a trigger mechanism in the immune response. Hyperreactivity of T cells and antibody production are features in pSS. ICOS can be critical in the pathogenesis of pSS. Methods: A total of 134 pSS patients and 134 control s...
Systemic sclerosis (SSc) is characterized by chronic inflammation and fibrosis, two processes associated with transforming growth factor β (TGF-β) functions. In the present study, we investigated the expression of TGF-β isoforms in serum and the skin distribution of TGF-β and two receptors (TGF-βR1 and TGF-βR2) in SSc patients. A total of 56 SSc pa...
Purpose
Understanding the humoral immune response dynamics carried out by B cells in COVID-19 vaccination is little explored; therefore, we analyze the changes induced in the different cellular subpopulations of B cells after vaccination with BNT162b2 (Pfizer-BioNTech).
Methods
This prospective cohort study evaluated thirty-nine immunized health w...
El Departamento de Neurociencias del Centro Universitario de Ciencias de la Salud presenta esta obra científico-académica que estudia los efectos de la enfermedad covid-19 sobre el sistema nervioso (afecciones también conocidas como “neurocovid”), a fin de comprender, actualizar y divulgar oportunamente el conocimiento sobre esta afectación, sus co...
Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed to evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 and 7) expression and sCD74 to establish their association with RA clinical activity according to DAS28-ESR.
Methodology:
101 RA patients with dif...
La salud y los derechos humanos se han vinculado de manera escasa y poco
explícita. Sin embargo, son enfoques modernos y poderosos para definir
y promover el bienestar humano. La atención a la intersección de la salud y
los derechos humanos puede proporcionar beneficios prácticos para quienes
se dedican específicamente a estas áreas. Además, puede...
We read the paper by Zhang et al. (1) with interest. The authors report on a study that evaluated the association of four long noncoding RNA (lncRNA) (ANRIL, lnc-DC, MALAT1, ZFAS1) gene single-nucleotide polymorphisms (SNPs) with susceptibility to rheumatoid arthritis (RA) patients, as well as their expression levels. They concluded that ANRIL, lnc...
Las enfermedades reumáticas autoinmunes son trastornos multisistémicos que afectan principalmente las articulaciones y los músculos; algunos ejemplos de estas afecciones son la artritis reumatoide, el lupus eritematoso sistémico y el síndrome de Sjögren. En general, las enfermedades reumáticas autoinmunes tienen una alta prevalencia en todo el mund...
Hasta julio de 2021, la actual pandemia por covid-19 ha cobrado cerca de
cuatro millones de muertes alrededor del mundo en tan solo 17 meses, de
acuerdo con lo reportado por el Centro de Ciencia e Ingeniería de Sistemas
de la Universidad Johns Hopkins. El curso clínico de esta enfermedad viral
difiere mucho de una persona a otra. Esta variación es...
Coronavirus disease 2019 (COVID-19) has emerged as a serious threat to global health. The disregulation of the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) cell signaling pathway observed in patients with COVID-19 has attracted attention for the possible use of specific inhibitors of this pathway for...
SARS-CoV-2 continues to infect thousands of people around the world. It has been established that the main transmission mechanism of this virus is via airborne route, which is why it initially infects the respiratory tract. Currently, the effectiveness of medications used against COVID-19 is limited, and although immunization programs have been ini...
Macrophage migration inhibitory factor (MIF) has been associated with the pathogenesis of several rheumatic diseases. In systemic sclerosis (SSc) it has been shown that MIF expression is dysregulated in serum and skin. However, the MIF receptor, CD74, has been poorly investigated and its potential role in the pathogenesis of SSc remains unknown. Th...
Background
Macrophage inhibitory factor (MIF) is a pro‐inflammatory cytokine secreted by several cells, including those in the immune system and the skin. The MIF gene contains the SNP ‐173 G> C and STR ‐794 CATT5‐8 polymorphisms in the promoter region capable of affecting its activity. Our objective was to investigate the MIF polymorphisms as a ri...
Developing countries have reported lower molecular diagnostic testing levels due to a lack of resources. Therefore, antibody tests represent an alternative to detect exposure to SARS-CoV-2 and analyze possible risk factors. We aimed to describe and compare the clinical-epidemiological characteristics and the quality of food intake in Mexican indivi...
There are few reports in oral squamous cell carcinoma (OSCC) that indicate the expression of macrophage migration inhibitory factor (MIF) in tissues, serum, or saliva of patients with OSCC. The aim of this study was to evaluate the mRNA expression and protein of MIF in tissues and serum, respectively, in OSCC patients and its association with the T...
Abstract: Background: The immunomodulatory effects of vitamin D are known to be beneficial in viral infections; it is also known that its deficiency is associated with a prognosis more critical of Coronavirus Disease 2019. This study aimed to determine baseline vitamin D serum concentrations and the effects of its supplementation in asymptomatic or...
Background
Several variants of the SARS-CoV-2 have been documented globally during the current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K SARS-CoV-2 mutations have been documented among the most relevant due to their potential pathogenic biological effects. This study aimed to design, validate, and propose a fast real-time RT-qPCR ass...
Currently, the world is under a pandemic of severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2), responsible for coronavirus disease 2019 (COVID‑19). This disease is characterized by a respiratory syndrome that can progress to an acute respiratory distress syndrome. To date, limited effective therapies are available for the prevention or t...
Background: Developing countries have reported lower molecular diagnostic testing levels due to a lack of infrastructure and resources. Therefore, antibody tests represent an alternative to detect exposure to SARS-CoV-2 and analyze possible risk factors in them. This study aimed to describe and compare the clinical and epidemiological characteristi...
Background
Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA.
Methods
This case‐control study incl...
After weeks under lockdown, metropolitan areas fighting the spread of COVID-19 aim to balance public health goals with social and economic standards for well-being. Mathematical models of disease transmission seeking to evaluate mitigation strategies must assess the possible impacts of social distancing, economic lockdowns and other measures. Howev...
The goals of our study were to determine the possible association of interleukin (IL)-31 with Th17 cytokine profile in serum and to quantify retinoic acid-related orphan receptor C ( RORC) mRNA expression in psoriatic arthritis (PsA) patients. This cross-sectional study was conducted in 50 patients with PsA and 30 control subjects (CS) matched by a...
Background
T‐cell activation pathways have been proposed as trigger mechanisms in the pathogenesis of rheumatoid arthritis (RA). CD28 and CTLA‐4 play major roles in regulating the stimulatory and inhibitory co‐signals in T cells.
Objective
To analyze the association between soluble and surface expression of CD28 and CTLA‐4 with the clinical parame...
Background:
Macrophage migration inhibitory factor (MIF) is a cytokine capable of stimulating inflammatory cytokine and matrix metalloproteinase production from macrophages and synovial fibroblasts, which leads to persistent inflammation and bone degradation, two of the major pathological processes in rheumatoid arthritis (RA). The aim of this stu...
Systemic sclerosis (SSc) is a rare autoimmune disease with high mortality, characterized by chronic inflammation and fibrosis, which are processes associated with higher serum tumor necrosis factor-α (sTNF-α) levels. TNFA -308G>A and -238G>A polymorphisms have been associated with higher sTNF-α levels. In this study, we genotyped the TNFA -308G>A a...
Background:
Cervical cancer (CC) is the second most common cancer in less developed countries and the second leading cause of death by cancer in women worldwide. The 99% of CC patients are infected with the Human Papilloma Virus (HPV), being HPV16 and HPV18 infection the most frequent. Even though HPV is considered to be a necessary factor for the...
INTRODUCTION: Systemic sclerosis (SSc) is a rare autoimmune disease with high mortality, characterized by chronic inflammation and fibrosis of the skin, vascular abnormalities and variable involvement of organs. Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine associated with inflammation and fibrosis process. Several studies have sug...
Introduction
Prolactin (PRL) is a sex hormone with immunomodulatory properties, and it is associated with the clinical activity of rheumatoid arthritis (RA). The -1149G>T polymorphism at the prolactin (PRL) gene has been associated with autoimmune diseases, but its functional effect is unclear.
Objective
To analyze the association of the PRL -1149...
Introduction
Systemic sclerosis (SSc) is a complex autoimmune disease, characterized by microvascular lesions, autoimmunity, and fibrosis. It is suggested that MIF participates in the amplification of the proinflammatory process in SSc; moreover, the promoter polymorphisms − 794 CATT5–8 (rs5844572) and − 173G>C (rs755622) in the MIF gene have been...
Background:
Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine that plays a crucial role as regulator of the innate and adaptive immune responses and takes part in the destructive process of the joint in rheumatoid arthritis (RA) by promoting angiogenesis and inducing proinflammatory cytokines and matrix metalloproteinase...
La misión y los objetivos de la medicina genómica son promover la incorporación de los hallazgos y descubrimientos de las ciencias genómicas a la práctica clínica. Para lograr esto, es preciso crear conciencia y educar a profesionales de la salud, tomadores de decisiones y a la población en general respecto del papel que desempeña esta ciencia emer...
Background
CD40 is a costimulatory molecule for B cells, and CD154 is a marker of CD4+ T cells activation. CD40‐CD154 interaction promotes pro‐inflammatory cytokines secretion and autoantibodies production. PTPN22 gene encodes LYP protein, an inhibitor of T‐ and B‐cell activation. PTPN22 1858C>T polymorphism confers rheumatoid arthritis (RA) suscep...
Introduction
Diabetic Nephropathy (DN) is the main cause of chronic kidney disease (CKD) in diabetic patients. An IL‐10 imbalance could be related to renal hypertrophy and trigger to nephropathy. Three promoter polymorphisms (‐1082G>A, ‐819C>T, and ‐592C>A) at IL10 gene have been associated with changes in the IL‐10 expression and DN susceptibility...
Las enfermedades cardiovasculares (ECV) representan una de las principales causas de muerte a nivel mundial. En la actualidad, se sabe que uno de los mecanismos patogénicos asociados a las ECV es la desregulación del metabolismo de las especies reactivas de oxígeno (estrés oxidativo).
La evaluación de los marcadores de estrés oxidativo, como las li...
INTRODUCTION:
Prolactin (PRL) is a 23-kDa protein that can be synthesized and secreted by pituitary and extrapituitary tissues such as immune cells due to its expression being regulated by two independent promoter regions. The promoter which is responsible for extrapituitary expression contains the single nucleotide polymorphism (SNP) -1149 G/T pre...
Objective
The aim of the present study was to evaluate the possible association between the Q223R Leptin receptor (LEPR) polymorphism (A>G; rs1137101) and leptin levels in patients with rheumatoid arthritis (RA) from Western Mexico.
Methods
A cross-sectional study was performed with 70 RA patients and 74 controls subject (CS). Disease activity was...
Psoriatic arthritis (PsA) is an autoimmune inflammatory disease associated with psoriasis. The cause of this pathology is still unknown, but research suggests the diseases are caused by a deregulated cytokine production. MIF is a cytokine associated with immunomodulation of Th1, Th2, and Th17 cytokine profiles in inflammatory diseases. Based on thi...
Se evaluó la relevancia clínica de analizar conjuntamente dos nuevos au-toanticuerpos (anti-vimentina citrulinada mutada: anti-MCV y anti-peptidil arginina desaminasa 4: anti-PAD4) en conjunto con los utilizados clásica-mente en el diagnóstico (factor reumatoideo: FR y anticuerpos contra pép-tidos citrulinados cíclicos de la anti-CCP) en la artriti...
El objetivo del presente estudio fue identificar perfiles de autoanticuerpos (FR, anti-CCP, anti-MCV y anti-PAD4) con funcionalidad diagnóstica y analizar su asociación con la actividad y severidad de la enfermedad en mestizos mexicanos con AR.
Rheumatoid arthritis (RA) is an autoimmune disease characterized by inflammation and pro-inflammatory cytokines production. IL-1Ra is an anti-inflammatory cytokine codified by IL1RN gene that blocks IL-1 signalling. A VNTR polymorphism of 86 bp in IL1RN gene has been associated with RA risk and regulation of IL-1Ra expression. In this study, we det...
The objective of this study was to determine the association of the CD40LG 3′-UTR (CA)n microsatellite with rheumatoid arthritis (RA) and CD40LG mRNA levels in females from western Mexico. A case-control study with 219 RA patients and 175 control subjects (CS) was conducted. Genotyping was performed by polymerase chain reaction (PCR), X2 test was u...
The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase – Lyp – and has been associated with different autoimmune disorders. The PTPN22 −1123G>C polymorphism appears to affect the transcriptional control of this gene, but to date, the biological...
Rheumatoid arthritis (RA) is a chronic, systemic disease of unknown etiology. Several studies have reported a variable number of tandem repeat (VNTR) 86 bp (rs2234663) in the intron 2 of IL1RN gene with RA risk. The present study was designed to determine the frequencies of this polymorphism in patients with RA and control subjects (CS) and its ass...
Background and objectives:
The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase -Lyp- and has been associated with different autoimmune disorders. The PTPN22 -1123G>C polymorphism appears to affect the transcriptional control of this gene, bu...
Rheumatoid arthritis (RA) is an autoimmune disease characterized by the presence of antibodies against cyclic citrullinated peptide (anti-CCP), a consequence of the breakdown of immune tolerance. The lymphoid tyrosine phosphatase (Lyp) protein has significant effects on maintenance of peripheral immune tolerance. Two polymorphic variants (−1123G>C...
Interleukin 10 (IL-10) is an immunoregulatory cytokine with multiple roles in the immune system. Three single nucleotide polymorphisms at positions -1082 (A>G), -819 (C>T), and -592 (C>A) in the promoter region of the IL10 gene are believed to be associated with different inflammatory, infectious, and autoimmune diseases. These polymorphisms exhibi...
The CD40 pathway is involved in the development and pathogenesis of autoimmune diseases, including rheumatoid arthritis (RA). Two single nucleotide polymorphisms (SNPs) in the CD40 gene, rs1883832 and rs4810485, are associated with susceptibility to inflammatory and autoimmune diseases and are thought to alter CD40 expression at the mRNA and protei...
Acute Myocardial Infarction (AMI) has the highest mortality rates in emergency services. In AMI pathophysiology, oxidative, inflammatory, metabolic, and thrombotic factors are involved. TNFA is a candidate gene for the disease, due to its involvement in the early stage of atherosclerotic plaque destabilization. The objective of this investigation w...
Acute Myocardial Infarction (AMI) has the highest mortality rates in emergency services. In AMI patho-physiology, oxidative, inflammatory, metabolic, and thrombotic factors are involved. TNFA is a candidate gene for the disease, due to its involvement in the early stage of atherosclerotic plaque destabilization. The objective of this investigation...
Ante una percepción general de inseguridad en la vida cotidiana, se ha reclamado desde la ciudadanía que se aborde de forma rápida e integral el fenómeno de la violencia. Esta obra surge de las contribuciones que ha hecho la comunidad del CUCS (UdeG), que en sus trabajos de tesis profesionales de grado y posgrado, analizan dicho fenómeno desde dife...
Primary Sjögreńs syndrome (pSS) is an autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands. Interleukin-10 (IL-10) plays a role in autoimmune diseases by promoting B-cell activation and autoantibodies production. IL10-1082A>G,-819C>T,-592C>A polymorphisms and their haplotypes have been associated with IL-10 p...
We analyzed the relationship of -794 CATT5-8 and -173 G>C MIF polymorphisms with mRNA and soluble MIF in young obese subjects. A total of 250 young subjects, 150 normal-weight and 100 obese subjects, were recruited in the study. Genotyping of -794 CATT5-8 and -173 G>C MIF polymorphisms was performed by PCR and PCR-RFLP, respectively. MIF mRNA expre...
Questions
Questions (3)
We want to perform a human RNA extraction from cell culture for an RNA-seq, but we have a viral RNA extraction kit (Quick-RNA™ Viral Kit-Zymo research) available. Therefore, we want to know if any methodological issues can interfere with the results if we use the viral kit.
qPCR Eficience Real-time PCR
I want to know which transcription factors bind to a specific region of the IL-10 promoter
