Jorge Ernesto González Mesa

Jorge Ernesto González Mesa
Center for Radiation Protection and Hygiene | CPHR · I+D+i

Jorge Ernesto González Mesa currently works at the I+D+I, Center for Radiation Protection and Hygiene. Jorge does resear

About

67
Publications
9,495
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Introduction
Jorge Ernesto González Mesa currently works at the I+D+I, Center for Radiation Protection and Hygiene. Jorge does research in Biological Dosimetry, Pharmacy, Toxicology, Molecular Biology and Cell Biology. Their most recent publication is 'Uncertainty calculation methods in dose assessment for dicentric chromosome assay'
Additional affiliations
October 2015 - October 2015
Clemente Estable Biological Research Institute
Position
  • Research Associate
July 2013 - July 2013
Clemente Estable Biological Research Institute
Position
  • Research Associate
Description
  • Micronucleus and gH2AX foci assay
September 2010 - December 2010
Autonomous University of Barcelona
Position
  • Fellow

Publications

Publications (67)
Article
To test a CellProfiler pipeline for automated counting and characterization of gamma-H2AX foci in color images of human cultured cells. A431 cells were irradiated and stained for gamma-H2AX foci detection. Sets of color images were analyzed visually, and findings were compared with those using FociCounter and CellProfiler software. The CellProfiler...
Article
Full-text available
The combination of automatic image acquisition and automatic image analysis of premature chromosome condensation (PCC) spreads was tested as a rapid biodosimeter protocol. Human peripheral lymphocytes were irradiated with 60Co gamma rays in a single dose of between 1 and 20 Gy, stimulated with phytohaemaglutinin and incubated for 48 h, division blo...
Article
Purpose: To present Poisson exact goodness-of-fit tests as alternatives and complements to the asymptotic u-test, which is the most widely used in cytogenetic biodosimetry, to decide whether a sample of chromosomal aberrations in blood cells comes from an homogeneous or inhomogeneous exposure. Materials and Methods: Three Poisson exact goodness-of-...
Article
Purpose: To present the impact in coverage of different methods for Poisson confidence intervals and the impact in dose coverage of different uncertainty factors. A detailed explanation of the uncertainty sources in the Bayesian method is also presented. Materials and methods: The exact coverage of uncertainty Poisson confidence intervals and the d...
Article
Purpose: To test the performance of different algorithms that can be used in interlaboratory comparisons based on dicentric chromosome analysis, and to evaluate the impact of considering a priori values different to calculate individual laboratory performance based on the ionizing radiation dose estimation. Methods: Mean and standard deviation e...
Article
Full-text available
Introducción: el laboratorio de citogenética del Instituto de Investigaciones en Salud (INISA) de la Universidad de Costa Rica estableció un Servicio de Dosimetría Biológica en enero del 2020 utilizando biomarcadores citogenéticos de exposición a radiaciones ionizantes. Es el primero de su tipo en la región centroamericana. Objetivo: establecer un...
Article
Full-text available
Resumen El Centro de Protección e Higiene de las Radiaciones fue fundado en 1985 para sustentar la aplica-ción segura de las tecnologías nucleares en Cuba. En la actualidad la institución es considerada como una referencia nacional y regional en materia de seguridad radiológica, gracias a una sólida estrategia institucional que aporta de manera sos...
Article
Purpose: This paper describes how the BioDoseNet Image Repository was used as a training tool for the dicentric assay. Materials and methods: The training was implemented in three phases, introduction to dicentric scoring, dose response curve elaboration and dose assessment exercise. Four labs without previous experience in the dicentric assay part...
Poster
Full-text available
COMPARATIVE ANALYSIS OF IRIDIUM-192 DOSE RESPONSE CURVES APPLIED IN THE BIOLOGICAL DOSIMETRY
Article
Purpose: The goal was to compare the micronucleus (MN) and dicentric plus ring chromosomes (D + R) assays for dose assessment in cases of partial body irradiations (PBI). Materials and methods: We constructed calibration curves for each assay at doses ranging from 0 to 5 Gy. In order to simulate partial-body exposures, blood samples from two donors...
Article
Purpose: This paper presents a calibration curve for Prematurely Condensed Chromosome Rings (PCC-ring) which are scarce in cytogenetic dosimetry; here we analyzed the behavior of the PCC-ring frequency and PCC index after gamma irradiation in the dose interval from 0 to 20 Gy. Materials and methods: PCC-rings were induced in lymphocytes with Calycu...
Article
The possibility to reduce the culture time and volume of blood and reagents required for the chemical Premature Chromosome Condensation (PCC) assay is demonstrated in this work. Peripheral whole blood was exposed to gamma radiation (1–20 Gy). Lymphocytes were cultured for 40 h, using 50 μl of blood and 450 μl of culture medium. The dose-response cu...
Article
Biological Dosimetry is a necessary support for national radiation protection programmes and emergency response schemes. The Latin American Biological Dosimetry Network (LBDNet) was formally founded in 2007 to provide early biological dosimetry assistance in case of radiation emergencies in the Latin American Region. Here are presented the main top...
Article
Mitotic Index (MI) estimation expressed as percentage of mitosis plays an important role as quality control endpoint. To this end, MI is applied to check the lot of media and reagents to be used throughout the assay and also to check cellular viability after blood sample shipping, indicating satisfactory/unsatisfactory conditions for the progressio...
Data
CellProfiler pipeline for foci analysis in DAPI-FITC images Applied in : J.E. González, M. Lee, J. F. Barquinero, M. Valente, S. Roch-Lefèvre, O.García. Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs. Analytical and quantitative cytology and histology 2012; 34(2):66-71.
Data
CellProfiler (www.cellprofiler.org) pipeline for Image Quality
Conference Paper
Full-text available
The cytokinesis-block micronucleus (CBMN) test is a quantitative assay for genetic toxicity assessment. One of the advantages of the MN assay is its amenability for automation. Different type of cells has been used to evaluate genetic damage through MN assay, such as, human lymphocytes and rodent cell lines (i.e. CHO, V79, CHL and L5178Y). The MN q...
Conference Paper
Full-text available
The algorithm Focus Score is tested to determine the quality of cytogenetic images transmitted electronically by each laboratory of the LBDNet.
Article
The fast assessment of the dose received by exposed persons is crucial in radiological accidents, so the 48 h of cell culture in conventional cytogenetic dosimetry in addition to some limitations after high doses becomes a disadvantage. The premature chromosome condensation (PCC) assay permits to analyse enough cells after high radiation exposure,...
Article
Analysis of premature chromosome condensation (PCC) mediated by fusion of G0-lymphocytes with mitotic CHO cells in combination with rapid visualization and quantification of rings (PCC-Rf) is proposed as an alternative technique for dose assessment of radiation-exposed individuals. Isolated lymphocytes or whole blood from six individuals were γ-irr...
Article
Full-text available
The aqueous standard extract of Mangifera indica L stem bark (MSBE) is used as a food supplement in Cuba. In this study, the genotoxic effect of MSBE was measured using different variants of the in vitro Comet assay in human lymphocytes and rat hepatocytes incubated with MSBE at 37C for 1 hour. Lymphocytes were incubated with MSBE for the subcellul...
Article
Full-text available
The estimation of the dose and the irradiated fraction of the body is important information in the primary medical response in case of a radiological accident. The PCC-R assay has been developed for high-dose estimations, but little attention has been given to its applicability for partial-body irradiations. In the present work we estimated the dos...
Article
Full-text available
The bottleneck in data acquisition during biological dosimetry based on a dicentric assay is the need to score dicentrics in a large number of lymphocytes. One way to increase the capacity of a given laboratory is to use the ability of skilled operators from other laboratories. This can be done using image analysis systems and distributing images a...
Article
Full-text available
Immunocompetent mice, Fc receptor γ-chain deficient mice (Fcer1g(-/-)), and molecular tools as F(ab')(2) bivalent fragments appear as the most suitable biological models to study the mechanisms of the action of anti-epidermal growth factor receptor (EGFR) monoclonal antibodies (mAbs). In vivo experiments contrasting antitumor effects of whole Abs a...
Article
The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A...
Article
Full-text available
Epidermal growth factor receptors (EGFR) are overexpressed in a wide range of malignancies including head and neck, colon, and breast cancers. It has been identified that carcinomas with high expression levels of EGFR are more resistant to radiotherapy. Therefore, inhibiting nuclear translocation of EGFR to increase the radiosensitivity of malignan...
Article
Biological dosimeters are recommended for dose estimation in case of human overexposure to ionising radiation. Rapid measurement of γ-H2AX foci as a marker of DNA double-strand breaks (DSB) induction has been recently tested with this purpose. Here we reported a dose-response relationship after X-ray irradiation at different times post-exposure. Bl...
Article
Full-text available
Calibration curves for fission spectrum neutrons and other high LET radiations are scarce in cytogenetic dosimetry and particularly for Prematurely Condensed Chromosome Rings (PCC-ring). Here we analyzed the behavior of the PCC-ring frequency and PCC index after neutron irradiation in a broad dose interval from 1 to 26 Gy. PCC-rings were induced in...
Article
Full-text available
In this paper we show the dose response curves in vitro obtained at first time in Cuba, with several biological indicators for radiation damage: rings in premature chromosome condensation (PCC-R); PCC index and -H2AX foci, all of these in peripheral human lymphocytes. These curves cover an interval of doses between 0.1 Gy to 25 Gy, several radiatio...
Article
Full-text available
A biomonitoring of workers of the pesticide formulation plant from Managua municipality to know if the manipulation of pesticides described as potential chemical mutagens, had a genotoxic effect was carried out. The primary damage of DNA was studied by means of the comet assay and the chromosomal damage by chromosomal aberrations in lymphocytes and...
Article
To study the maternofetal and milk transfer of an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb) and its effects on conceptus, we administered to pregnant and lactating dams the murine anti-EGFR MAb 7A7 in an autologus model. For determining the embryo-fetal toxicity, 7A7 at gestational days (GD) 6, 8, 12 and 14 were intrave...
Article
One of the difficulties in the comparison of results between laboratories working with the comet assay is the great diversity of parameters used to express DNA damage and the lack of conversion factors between the majority of them. Here we report a scorer-independent conversion curve to transform the values of DNA damage reported in arbitrary units...
Article
Full-text available
1E10 is a murine anti-idiotypic mAb specific for an idiotypic mAb that reacts with NeuGc-containing gangliosides, sulfatides, and Ags expressed in some human tumors. In melanoma, breast, and lung cancer patients, this anti-idiotypic Ab was able to induce a specific Ab response against N-glycosylated gangliosides, attractive targets for cancer immun...
Article
Full-text available
Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization is essential if a cooperative network is used to respond to a mass casualty event. Here we present an international interco...
Article
Full-text available
ABSTRACT. D-003 is a mixture of higher aliphatic fatty acids purified from the sugarcane wax (Saccharum officinarum L.) with cholesterol-lowering and antiplatelet effects that reduces the concentration of thromboxane B2 and increases those of prostacyclin in plasma, which indirectly suggests that D-003 produces a beneficial effect on the vascular e...
Article
To test the gamma-H2AX (Histone 2AX phosphorylation of serine 139) foci assay for the detection of ionising radiation-induced DNA damage in buccal exfoliated cells. Buccal mucosa cells from five individuals (three females, two males, aged 26-47 years) were exposed to 0, 0.5, 1, 2 and 4 Gy of gamma-rays. DNA damage and DNA damage removal were measur...
Article
Recent studies have suggested that visualization of gamma-H2AX nuclear foci can be used to estimate exposure to very low doses of ionizing radiation. Although this approach is widely used for various purposes, its suitability for individual human biodosimetry has not yet been assessed. We therefore conducted such an assessment with the help of avai...
Article
Full-text available
INTRODUCTION: The aqueous extract bark of Rhizophora mangle L. (red mangrove) has traditionally been used in Cuban folk medicine due to its wide array of curative properties: astringent, haemostatic, febrifuge and antifungal. Previous chemical characterization studies of the liophylized aqueous extract bark, revealed that tannins are the main compo...
Article
Full-text available
D-003 is a mixture of very high molecular weight aliphatic primary acids purified from sugarcane wax, with antiosteoporotic, cholesterol-lowering and antioxidant effects. This study investigated the in vitro genotoxic potential of D-003 to produce chromosomal aberrations in CHO cells culture. Fresh suspensions of D-003 were added 50, 500, 1000, 250...
Article
Full-text available
The in vivo genotoxic effect of ozone/oxygen mix (O2-O 3) was studied in leukocytes and exfoliated colorectal cells of SD rats using the Comet assay (single cell gel electrophoresis assay, SCGE). O 2-O3 (O3 final dose 42 g/kg b.w.) was applied for four days by colorectal insufflation simulating human ozone therapy. Bleomycin (15 mg/kg b.w., i.p.) w...
Article
Mango (Mangifera indica L.) stem bark aqueous extract (MSBE) is a new natural product with antioxidant, anti-inflammatory and immunomodulatory effects known by the brand name of its formulations as Vimang. Previously, the oral toxicity studies of the extract showed a low toxicity potential up to 2000 mg/kg. This work reports the results about terat...
Article
Silver stain offers the possibility to stain comets permanently, but up to now it was impossible to measure the majority of the comet parameters, because the distinction between head and tail was not recognised by software. Here, we report a silver staining protocol that allows the measurement of comet parameters using the free Internet software CA...
Article
Full-text available
Mangifera indica L. extract (Vimang) consists of a defined mixture of components (polyphenols, terpenoids, steroids, fatty acids and microelements). It contains a variety of polyphenols, phenolic esters, flavan-3-ols and a xanthone (mangiferin), as main component. This extract has antioxidant action, antitumor and immunemodulatory effects proved in...
Article
D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax with cholesterol-lowering and antiplatelet effects. In order to further characterize the developmental toxicity during the treatment period from late gestation up to weaning of the offspring, pregnant females received 0 (control), 500, and 1,000 mg/kg/day D-003 d...
Article
Full-text available
El D-003 es una mezcla de ácidos alifáticos primarios de muy alto peso molecular purificada de la caña de azúcar (Saccharum officinarum) con efecto hipolipemiante y antiagregante plaquetario. Estudios previos de toxicología experimental han mostrado ausencia de toxicidad asociada al D- 003 administrado como dosis únicas o repetidas durante corto o...
Article
D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax with cholesterol-lowering properties. D-003 given orally (500 and 1000 mg/kg/day) to female rats for 15 days prior to mating, through mating and gestation to day 21 of lactation and male rats for 4 weeks prior and during mating did not induce toxic effects on rep...
Article
Nineteen scorers from seven Cuban laboratories participated in this slide exercise designed to test the influence of the scorer on the accuracy, sensitivity and variability of the comet assay when a visual method of DNA damage evaluation is used. The assay was performed using human lymphocytes from a single donor exposed in vitro for 5 min at 0 deg...
Article
Full-text available
D-003 is a mixture of very-long-chain aliphatic acids with cholesterol-lowering and concomitant anti-platelet effects. The microsomal cytochrome P-450 system comprises a superfamily of proteins present in hepatic and extrahepatic tissues that is responsible for the metabolism of many drugs. The present study was undertaken to investigate the effect...
Article
Osteoporosis is characterized by reduced bone mass, abnormal bone architecture and increased fracture risk. Ovariectomy impairs bone mass and metabolism in rats and ovariectomized rats are considered as a suitable model of postmenopausal osteoporosis. Mevalonate is required for producing lipoids that are important in osteoclast activity and thus dr...
Article
D-003 is a mixture of high molecular weight aliphatic primary acids purified from sugar cane wax (Saccharum officinarum, L) with cholesterol-lowering and antiplatelet effects. Previous studies, including a 6-month study conducted in rats, have shown no D-003-related toxicity. The present study was undertaken to investigate the effects of D-003 oral...
Article
D-003 is a natural mixture of long chain aliphatic acids isolated and purified from sugar cane wax. It possesses antiplatelet and antithrombotic effects as well as decreases plasma and serum levels of thromboxane B(2) (TxB(2)), meanwhile significantly and markedly raises prostacyclin (PgI(2)) levels in rats. This study was undertaken to investigate...
Article
Full-text available
ABSTRACT. D-003 is a mixture of very long-chain aliphatic acids purified from sugar cane wax with cholesterol-lowering effects proved in different experimental models. The toxicological studies performed up to date indicate the low toxicity potential of this product. The present study was undertaken to research the in vivo genotoxic potential of D-...
Article
D-003 is a mixture of high-molecular-weight aliphatic primary acids purified from sugar cane wax with antiplatelet and cholesterol-lowering effects. Cardiac lesions induced by isoproterenol (ISO) are characterized by myocardial necrosis and exudative infiltration. The objective of this study was to determine whether D-003 shows protective effects a...
Article
D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax, the major component of which is 1-octacosanoic acid and which possesses effective antiplatelet, antithrombotic and cholesterol-lowering effects. D-003 was suspended in 1% acacia gum solution, and given daily by gavage to rats at dose levels of 5, 100 and 1000 mg...
Article
D-003 is a mixture of high molecular weight aliphatic primary acids purified from sugarcane wax (Saccharum officinarum) having cholesterol-lowering and antiplatelet effects. This study was undertaken to investigate the toxicity induced by long-term oral administration of D-003 for 6 months to Sprague Dawley rats of both sexes. Rats were randomly di...
Article
D-003 is a mixture of very long chain aliphatic acids purified from sugar cane wax with cholesterol-lowering effects. The present study was undertaken to investigate the in vivo cytotoxic and genotoxic potential of D-003 using three established assays: bone marrow micronucleus, sperm morphology, and single cell gel electrophoresis (Comet) assay. In...

Questions

Questions (2)
Question
Sampling error variation seems to high at the higher doses, more than 2 Gy.

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Projects

Projects (4)
Project
Under some assumptions exist numerical agreement with advantages for Bayesian method around 0.1 Gy Dose.
Project
To explore and testing robust algorithms for laboratory comparisons with limited numbers of participants. To explore and testing glm robust algorithms for calibration of dicentric assay. Based in R.