Jorge Galindo-Villegas

Nord University | HIBO · Faculty of Biosciences and Aquaculture

A key goal of a successful vaccine formulation is the strong induction of persistent protective immune responses without producing side-effects. Adjuvants have been proved to be successful in several species at inducing increased immune responses against poorly immunogenic antigens. Fish are not the exception and promising results of adjuvanted vac...

As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos. Mechanistically, skin keratinocytes were responsible for both sen...

Background Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs p...

Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened healthcare system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for developing new technologies and therapies for the best clinical management of pat...

Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by...

Muscle growth in teleosts is a complex biological process orchestrated by numerous protein-coding genes and non-coding RNAs. A few recent studies suggest that circRNAs are involved in teleost myogenesis, but the molecular networks involved remain poorly understood. In this study, an integrative omics approach was used to determine myogenic circRNAs...

Muscle growth in teleosts is a complex biological process orchestrated by numerous protein-coding genes and non-coding RNAs. A few recent studies suggest that circRNAs are involved in teleost myogenesis, but the molecular networks involved remain poorly understood. In this study, an integrative omics approach was used to determine myogenic circRNAs...

Foreword to "The 4th ISFSI Congress Bodø, Norway (December 11th – 15th, 2022)" - Keynote Speakers Abstracts DOI: 10.1016/j.fsi.2022.10.043 - Oral Abstracts DOI: 10.1016/j.fsi.2022.10.042 - Poster Abstracts DOI: 10.1016/j.fsi.2022.10.044

Background: Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. The tenth of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circ...

Aquaculture is a growing industry where facilities require robust strategies to help prevent the spread of infectious diseases. Using effective biocides as disinfectants in biosecurity plans is essential to implement a comprehensive safety program. Although the majority of high-level commercial biocides acting effectively on the free-living plankto...

Pacific herring (Clupea pallasii) is an essential target of commercial fishing in the North Pacific Ocean. Previous studies have suggested the existence of marine and lake ecological forms of this species within its range. The lake ecological form of herring has a shortened life cycle, spending the winter and spawning in brackish waters near the sh...

Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for the development of new technologies and therapies for the best clinical manageme...

Regulation of inflammation is a critical process for maintaining physiological homeostasis. The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circula...

This work presents the first study showing how photobiomodulation (PBM) significantly increases cellular and tissue repair and elucidating the role of PBM with low-level laser as a possible new therapy in pathologies in COVID-19-associated cytokine storm syndrome from a zebrafish model. Our results demonstrate new strategies for treating SARS-COV-2...

Interspecific hybridization has occurred relatively frequently during the evolution of vertebrates. This process usually abolishes reproductive isolation between the parental species. Moreover, it results in the exchange of genetic material and can lead to hybridogenic speciation. Hybridization between species has predominately been observed at the...

05.- Editorial: 10.- Diet, Immunity, and Microbiota Interactions: An Integrative Analysis of the Intestine Transcriptional Response and Microbiota Modulation in Gilthead Seabream (Sparus aurata) Fed an Essential Oils-Based Functional Diet 29.- Carvacrol, Thymol, and Garlic Essential Oil Promote Skin Innate Immunity in Gilthead Seabream (Sparus a...

Bacillus spp. supplementation as probiotics in cultured fish diets has a long history of safe and effective use. Specifically, B. velezensis show great promise in fine-tuning the European sea bass disease resistance against the pathogenicity caused by several members of the Vibrio family. However, the immunomodulatory mechanisms behind this respons...

Peracetic acid (PAA) is a European Union Commission-authorized disinfectant for use in animal health care. It has shown a strong inactivation potential for bacteria, viruses, fungi, and bacterial spores. A stress-related adaptive response after exposure to PAA has been described in different species of fish such as carp (Cyprinus carpio), rainbow t...

Aquaculture is recognized globally as the fastest growing food-producing sector for human consumption (1). However, to satisfy increasing international demand, it is predicted that current production must be doubled by 2030 while improving the biosafety protocols (2). Such an expansion will necessarily involve implementing innovative actions in aqu...

Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Dani...

In fish culture settings, the exogenous input of steroids is a matter of concern. Recently, we unveiled that in the gilthead seabream (Sparus aurata), the G protein-coupled estrogen receptor agonist G-1 (G1) and the endocrine disruptor 17a-ethinylestradiol (EE 2) are potent modulators in polyreactive antibody production. However, the integral role...

Therapeutic bacteriophages, commonly called as phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms including cultured fish. Their natural immunogenicity often induces the modulation of a variated collection of immune responses within several types of immunocytes while p...

Within the food-producing sectors, aquaculture is the one that has developed the greatest growth in recent decades, currently representing almost 50% of the world’s edible fish. The diseases can affect the final production in intensive aquaculture; in seabass, aquaculture vibriosis is one of the most important diseases producing huge economical los...

Circular RNAs (circRNAs) are an emerging class of regulatory RNAs with a covalently closed-loop structure formed during pre-mRNA splicing. Recent advances in high-throughput RNA sequencing and circRNA-specific computational tools have driven the development of novel approaches to their identification and functional characterization. CircRNAs are st...

The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpol...

Aquaculture growth will unavoidably involve the implementation of innovative and sustainable production strategies, being functional feeds among the most promising ones. A wide spectrum of phytogenics, particularly those containing terpenes and organosulfur compounds, are increasingly studied in aquafeeds, due to their growth-promoting, antimicrobi...

The variated environmental stimuli interacting at the mucosal surfaces on vertebrates are critical threats affecting their health status. However, the long-term effects and the possible heritability of the same have not been approached. This study sought to investigate whether a lack of bacterial priming in newly hatched zebrafish (ZF) embryos affe...

Genetic selection in gilthead seabream (GSB), Sparus aurata, has been undertaken to improve the growth, feed efficiency, fillet quality, skeletal deformities and disease resistance, but no study is available to delineate the effect of genetic selection for growth trait on GSB reproductive performance under mass spawning condition. In this study, hi...

Interspecies hybridization is driven by a complex interplay of factors where introgression plays an important role. In the present study, the transfer of genetic material, between two quite distant fish species from different genera, through spontaneous hybridization was documented with dedicated molecular and bioinformatics tools. We investigate t...

About this Special Issue: Orally administered health promoters are critical mediators in enhancing the fish immune system. The growing worldwide interest in reducing synthetic chemical application in aquaculture production has resulted in an overwhelming cumulus of reports describing various enhancers beneficial properties from products with a nat...

The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpol...

In the aquaculture industry, fish are stunned using a wide range of methods, but all of them trigger stress responses and affect the fish flesh quality. Chilled water is considered one of the most efficient methods, but even this is not a stress-free experience for the fish. Anesthetics included in the ice slurry or in water could decrease this str...

Interspecies hybridization is driven by a complex interplay of factors where introgression plays an important role. In the present study, the transfer of genetic material, between two quite distant fish species from different genera, through spontaneous hybridization was documented with dedicated molecular and bioinformatics tools. We investigate t...

Establishing new experimental animal models to assess the safety and immune response to the antigen used in the development of COVID-19 vaccine is an imperative issue. Based on the advantages of using zebrafish as a model in research, herein we suggest doing this to test the safety of the putative vaccine candidates and to study immune response aga...

The use of effective biocides as disinfectants is essential in aquaculture facilities. However, while most biocides act effectively on free-living planktonic pathogens, they are seldom useful against biofilms. In this study, we evaluate the biocidal efficacy and antimicrobial specific contact time of three disinfectants, Virkon™Aquatic (VirA), pera...

Fungi, particularly yeasts, are known essential components of the host microbiota but their functional relevance in the development of immunity and physiological processes of fish remains to be elucidated. In this study, we used a transcriptomic approach and a germ-free (GF) fish model to determine the response of newly hatched zebrafish larvae aft...

We are in the middle of a pandemic where time is a critical factor. A large panel of established pharmacological drugs seems useful to fight the SARS-CoV-2 virus and the resulting Covid-19 disease. Despite the emergency state, testing the same straight in humans is highly questionable not to say extremely risky. Thus, animal models of human disease...

In aquaculture, to minimize the negative side-effects produced by the intrinsic and variated fish management practices, required throughout the farming process, the use of anesthetics is recommended. Paradoxically, adverse side effects resulting from the incorrect management of the same in species-specific domesticated fish have been extensively re...

Significance Many pathogens exploit the olfactory route to gain entry into the host. Olfactory sensory neurons (OSNs) are responsible for the detection of chemical stimuli in the environment but are also continuously exposed to microorganisms. Here, we report the interaction between crypt neurons (unique OSNs present in fish) and fish rhabdoviruses...

Executive Summary Background Cost-effective commercial production of marine fish larvae is still a bottleneck in European aquaculture. The high and unpredictable mortality (sometimes more than 80%) in the first weeks after hatching remains a challenging problem that needs to be solved. The severity of the problem differs between species, but cannot...

The nervous system is known to regulate host immune responses. However, the ability of neurons to detect danger and initiate immune responses at barrier tissues is unclear. Vertebrate olfactory sensory neurons (OSNs) are located in direct contact with the external environment and therefore are directly exposed to pathogens. Here, we report that nas...

Establishment of the early-life gut microbiota has a large influence on host development and succession of microbial composition in later life stages. The effect of commensal yeastswhich are known to create a conducive environment for beneficial bacteriaon the structure and diversity of fish gut microbiota still remains unexplored. The present st...

Vertebrate olfactory sensory neurons (OSNs) are located in direct contact with the external environment and therefore they directly exposed to microorganisms such as viruses. Teleost fish have four types of OSNs, including crypt neurons, a cell type with enigmatic function. Crypt neurons express tyrosine receptor kinase A (TrkA)-like molecule and O...

Vertebrate olfactory sensory neurons (OSNs) are located in direct contact with the external environment and therefore they directly exposed to microorganisms such as viruses. Teleost fish have four types of OSNs, including crypt neurons, a cell type with enigmatic function. Crypt neurons express tyrosine receptor kinase A (TrkA)-like molecule and O...

Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics...

Microbiology and immunology of fish larvae

During the early ontogeny of fish a variety of characteristics are determined as a response to environmental conditions and genetic background. As a lot of these characters are directly or indirectly related with the quality of on-growing fish, there is an urgent need to develop appropriate indices for the description and prediction of fish quality...

Three different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilt-head sea bream IgM and IgT in their membrane (m) and soluble (s) forms are desc...

Galindo-Villegas, J., A. Montalban-Arques, S. Liarte, S. de Oliveira, C. Pardo-Pastor, F. Rubio-Moscardo, J. Meseguer, M. A. Valverde, and V. Mulero. 2016. TRPV4-mediated detection of hyposmotic stress by skin keratinocytes activates developmental immunity. J . Immunol . 196: [738–749][1]. A

All animals develop in association with complex microbial communities. It is now well established that commensal microbiota is essential for the correct functionality of each organ in the host. Particularly, the commensal gastro-intestinal microbiota (CGIM) is a key factor for development, immunity and nutrient conversion, rendering them bio-availa...

A potential concern of fish culturists is to reduce any economic loss. In European sea bass (Dicentrarchus labrax L.) aquaculture rearing facilities, mostly at those located in southern European countries, infectious diseases are a leading cause of mortality. Bacterial and viral diseases of sea bass (the most recurrent namely vibriosis, pasteurello...

The natural killer group 2 members D (NKG2D) is an activating receptor which plays a major role in immune surveillance, and the detection and elimination of malignant tumors and infected cells. NKG2D acts over both arms of the vertebrate immune response, and is expressed in some human and mouse myelopoietic, γδ T, NKT and CD4+ cells, but is present...

Vibrio anguillarum is the main causative agent of vibriosis in cultured sea bass. Unfortunately, available vaccines against this disease do not achieve the desired protection. In this study, to accomplish uptake, processing, and presentation of luminal antigens, a commercial sea bass oral vaccine against V. anguillarum was improved with the additio...