Joji Grace Villamor

Joji Grace Villamor
Max Planck Institute for Plant Breeding Research | MPIPZ · Department of Plant Microbe Interactions

PhD

About

12
Publications
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778
Citations
Citations since 2017
0 Research Items
521 Citations
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2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080

Publications

Publications (12)
Article
Full-text available
Proline accumulation is one of the most prominent changes in plant metabolism during drought and low water potential; however, regulation and function of proline metabolism remain unclear. We used a combination of forward genetic screening based on a Proline Dehydrogenase1 (PDH1) promoter-luciferase reporter (PDH1pro:LUC2) and RNA sequencing of the...
Article
Full-text available
Plants produce hundreds of glycosidases. Despite their importance in cell wall (re)-modelling, protein and lipid modification and metabolite conversion, very little is known of this large class of glycolytic enzymes, partly because of their post-translational regulation and their elusive substrates. Consequently, glycosidases are poorly defined in...
Article
Full-text available
The herbicides glyphosate (Gly) and imazamox (Imx) inhibit the biosynthesis of aromatic and branched-chain amino acids, respectively. Although these herbicides inhibit different pathways, they have been reported to show several common physiological effects in their modes of action, such as increasing free amino acid contents and decreasing soluble...
Article
Full-text available
Many protein activities are driven by ATP binding and hydrolysis. Here, we explore the ATP-binding proteome of the model plant Arabidopsis thaliana using Acyl-ATP (AcATP) probes. These probes target ATP binding sites and covalently label lysine residues in the ATP binding pocket. Gel-based profiling using biotinylated AcATP showed that labeling is...
Article
Chemical probes have great potential for identifying functional residues in proteins in crude proteomes. Here we studied labeling sites of chemical probes based on sulfonyl fluorides (SFs) on plant and animal proteomes. Besides serine proteases and many other proteins, SF-based probes label Tyr residues in glutathione transferases (GSTs). The label...
Article
Full-text available
The Arabidopsis thaliana accession Shahdara (Sha) differs from Landsberg erecta (Ler) and other accessions in its responses to drought and low water potential including lower levels of proline accumulation. However, Sha maintained greater seedling root elongation at low water potential and a higher NADP/NADPH ratio than Ler. Profiling of major amin...
Article
Full-text available
Drought-induced proline accumulation is widely observed in plants but its regulation and adaptive value are not as well understood. Proline accumulation of the Arabidopsis accession Shakdara (Sha) was threefold less than that of Landsberg erecta (Ler) and quantitative trait loci mapping identified a reduced function allele of the proline synthesis...
Article
Full-text available
To better define the still unclear role of proline (Pro) metabolism in drought resistance, we analyzed Arabidopsis (Arabidopsis thaliana) Δ(1)-pyrroline-5-carboxylate synthetase1 (p5cs1) mutants deficient in stress-induced Pro synthesis as well as proline dehydrogenase (pdh1) mutants blocked in Pro catabolism and found that both Pro synthesis and c...
Article
Matrix metalloproteases (MMPs) are secreted or membrane-bound zinc-containing proteases that play diverse roles in development and immunity in plants and in tissue remodeling in animals. We developed a photoreactive probe based on the MMP inhibitor marimastat, conjugated to a 4-azidotetrafluorobenzoyl moiety as photoreactive group and biotin as det...
Article
本研究透過順向遺傳學及逆向遺傳學研究策略,篩得一些T-DNA插入突變株。為了探討水稻轉錄因子APETALA2 (AP2) 基因的功能,利用逆向遺傳學的策略,篩得水稻T-DNA插入突變株M38333,其T-DNA插入水稻BAC OSJNBa0044E16第37,503 bp處,位於預測為AP2基因下游5,705 bp。M38333外表性狀與TNG67比較,呈現半矮性、開花延遲及在水稻劍葉lamina joint處傾斜下垂。分析T-DNA插入鄰近基因之表現,發現333-07 (unknown protein) 及333-09 (putative AP2 protein) 兩個基因有被活化大量表現,推測M38333的外表性狀特異可能藉由活化此兩基因所造成,目前正進一步構築大量表現此兩基因的水稻轉殖...
Article
本研究透過順向遺傳學及逆向遺傳學研究策略,篩得一些T-DNA插入突變株。為了探討水稻轉錄因子APETALA2 (AP2) 基因的功能,利用逆向遺傳學的策略,篩得水稻T-DNA插入突變株M38333,其T-DNA插入水稻BAC OSJNBa0044E16第37,503 bp處,位於預測為AP2基因下游5,705 bp。M38333外表性狀與TNG67比較,呈現半矮性、開花延遲及在水稻劍葉lamina joint處傾斜下垂。分析T-DNA插入鄰近基因之表現,發現333-07 (unknown protein) 及333-09 (putative AP2 protein) 兩個基因有被活化大量表現,推測M38333的外表性狀特異可能藉由活化此兩基因所造成,目前正進一步構築大量表現此兩基因的水稻轉殖...

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