Johanna H KattenbergInstitute of Tropical Medicine | ITM · Department of Biomedical Sciences
Johanna H Kattenberg
BSc MSc PhD
About
61
Publications
4,352
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Introduction
Johanna H Kattenberg currently works at the Department of Biomedical Sciences, Institute of Tropical Medicine.
Current research topics:
- Capacity building in molecular biology research and tools
- Malaria molecular epidemiology and population genetics.
Development of tools for molecular surveillance
- Malaria transmission dynamics and tracing routes of infections
- Emergence and spread of drug resistance and resistance markers
- P. vivax biology: invasion and transcriptomics
Previous topics include:
- Diagnosis of malaria, in particular in malaria in pregnancy
- In vitro drug sensitivity and discovery of drug resistant markers
Additional affiliations
April 2016 - present
October 2012 - March 2016
December 2007 - June 2012
Publications
Publications (61)
Background
Low-density and asymptomatic Plasmodium vivax infections remain largely undetected and untreated and may contribute significantly to malaria transmission in the Amazon.
Methods
We analysed individual participant data from population-based surveys that measured P vivax prevalence by microscopy and polymerase chain reaction (PCR) between...
Background
Malaria molecular surveillance has great potential to support local national malaria control programs (NMCPs) to inform policy for malaria control and elimination. Molecular markers associated with drug resistance are good predictors of treatment responses. In addition, molecular detection of deletions in hrp2 and hrp3 genes are indicati...
We report an outbreak investigation of two fatal cases of autochthonous Plasmodium falciparum that occurred in Belgium in September 2020. Various hypotheses of potential source of infection were investigated. Based on the collected information, the most likely route of transmission was through an infectious exotic Anopheles mosquito that arrived vi...
Background
Understanding epidemiological variables affecting gametocyte carriage and density is essential to design interventions that most effectively reduce malaria human-to-mosquito transmission.
Methodology/Principal findings
Plasmodium falciparum and P . vivax parasites and gametocytes were quantified by qPCR and RT-qPCR assays using the same...
Chloroquine (CQ) is the first-line treatment for Plasmodium vivax malaria in most endemic countries. Monitoring P.vivax CQ resistance (CQR) is critical but remains challenged by the difficulty to distinguish real treatment failure from reinfection or liver relapse. Therapeutic efficacy of CQ against uncomplicated P.vivax malaria was evaluated in Gi...
Chloroquine (CQ) is the first-line treatment for Plasmodium vivax malaria in most endemic countries. Monitoring P.vivax CQ resistance (CQR) is critical but remains challenged by the difficulty to distinguish real treatment failure from reinfection or liver relapse. Therapeutic efficacy of CQ against uncomplicated P.vivax malaria was evaluated in Gi...
Background
Genomic surveillance of malaria parasite populations has the potential to inform control strategies and to monitor the impact of interventions. Barcodes comprising large numbers of single nucleotide polymorphism (SNP) markers are accurate and efficient genotyping tools, however may need to be tailored to specific malaria transmission set...
Monitoring the genetic structure of pathogen populations may be an economical and sensitive approach to quantify the impact of control on transmission dynamics, highlighting the need for a better understanding of changes in population genetic parameters as transmission declines. Here we describe the first population genetic analysis of the major hu...
Background:
In the past decade, national malaria control efforts in Papua New Guinea (PNG) have received renewed support, facilitating nationwide distribution of free long-lasting insecticidal nets (LLINs), as well as improvements in access to parasite-confirmed diagnosis and effective artemisinin-combination therapy in 2011-2012.
Methods:
To st...
Background:
Artemisinin-based combination therapies (ACTs) have significantly contributed to reduce Plasmodium falciparum malaria burden in Vietnam, but their efficacy is challenged by treatment failure of dihydroartemisinin/piperaquine ACT in Southern provinces.
Objectives:
To assess the efficacy of dihydroartemisinin/piperaquine for uncomplica...
Background
In the past decade, national malaria control efforts in Papua New Guinea (PNG) have received renewed support, facilitating nationwide distribution of free long-lasting insecticidal nets (LLINs), as well as improvements in access to parasite-confirmed diagnosis and effective artemisinin-combination therapy in 2011-12.
Methods
To study the...
Introduction:
As malaria transmission declines, understanding the differential impact of intensified control on Plasmodium falciparum relative to Plasmodium vivax and identifying key drivers of ongoing transmission is essential to guide future interventions.
Methods:
Three longitudinal child cohorts were conducted in Papua New Guinea before (200...
Monitoring the genetic structure of malaria parasite populations has been proposed as a novel and sensitive approach to quantify the impact of malaria control and elimination efforts. Here we describe the first population genetic analysis of sympatric Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) populations following nationwide distribution...
Background:
Infection during pregnancy with Plasmodium falciparum is associated with maternal anaemia and adverse birth outcomes including low birth weight (LBW). Studies using polymerase chain reaction (PCR) techniques indicate that at least half of all infections in maternal venous blood are missed by light microscopy or rapid diagnostic tests....
Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was...
Longitudinal tracking of individual Plasmodium falciparum strains in multi-clonal infections is essential for investigating infection dynamics of malaria. The traditional genotyping techniques did not permit tracking changes in individual clone density during persistent natural infections. Amplicon deep sequencing (Amp-Seq) offers a tool to address...
Background:
In 2009, the Papua New Guinea (PNG) Department of Health adopted artemether-lumefantrine (AL) and dihydroartemisinin-piperaquine (DHA-PPQ) as the first- and second-line treatments for uncomplicated malaria, respectively. This study was conducted to assess the efficacy of both drugs following adoption of the new policy.
Methods:
Betwe...
Background:
In Vietnam, malaria transmission has been reduced to very low levels over the past 20 years, and as a consequence, the country aims to eliminate malaria by 2030. This study aimed to characterize the dynamics and extent of the parasite reservoir in Central Vietnam, in order to further target elimination strategies and surveillance.
Met...
Background
Longitudinal tracking of individual Plasmodium falciparum strains in multi-clonal infections is essential for investigating infection dynamics of malaria. The traditional genotyping techniques did not permit tracking changes in individual clone density during persistent natural infections. Amplicon deep sequencing (Amp-Seq) offers a tool...
Background:
In Vietnam, malaria persists in remote forested regions where infections are spatially heterogeneous, mostly asymptomatic and with low parasite density. Previous studies in Vietnam have investigated broad behavioural concepts such as 'engaging in forest activities' as risk factors for malaria infection, which may not explain heterogene...
Background
Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections. New amplicon sequencing and data analysis protocols are needed for genotyping in epidemiological studies and drug efficacy trials of P. falciparum.
Methods
Targeted sequen...
Background:
The scale-up of effective malaria control in the last decade has resulted in a substantial decline in the incidence of clinical malaria in many countries. The effects on the proportions of asymptomatic and submicroscopic infections, and on transmission potential are yet poorly understood.
Methods:
In Papua New Guinea, vector control...
Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections. Such high resolution is needed for molecular monitoring of drug efficacy trials. Targeted sequencing of molecular marker csp and novel marker cpmp was conducted in duplicate on mixtur...
Background:
The diagnosis of malaria during pregnancy is complicated by placental sequestration, asymptomatic infection, and low-density peripheral parasitaemia. Where intermittent preventive treatment (IPT) with sulfadoxine-pyrimethamine is threatened by drug resistance, or is inappropriate due to low transmission, intermittent screening and trea...
Background
In northern Papua New Guinea (PNG), most Plasmodium falciparum isolates proved resistant to chloroquine (CQ) in vitro between 2005 and 2007, and there was near-fixation of pfcrt K76T, pfdhfr C59R/S108N and pfmdr1 N86Y. To determine whether the subsequent introduction of artemisinin combination therapy (ACT) and reduced CQ-sulphadoxine-py...
Background:
Artemisinin combination therapies (ACTs) with broad efficacy are needed where multiple Plasmodium species are transmitted, especially in children, who bear the brunt of infection in endemic areas. In Papua New Guinea (PNG), artemether-lumefantrine is the first-line treatment for uncomplicated malaria, but it has limited efficacy agains...
Rapid diagnostics tests (RDTs) detect malaria specific antigen(s) in the circulation, even when parasites are sequestered in the placenta and not visible by microscopy. However, research on their diagnostic accuracy during pregnancy is limited. Pregnant women (n = 418) were screened for malaria during routine antenatal care by using two RDTs that d...
Objectives:
To evaluate persistence of several Plasmodium antigens in pregnant women after treatment and compare diagnostics during treatment follow-up.
Methods:
Thirty-two pregnant women (N = 32) with confirmed malaria infection by a histidine-rich protein 2 (HRP2)-based rapid diagnostic test (RDT) and microscopy were followed for 28 days after...
Currently available rapid diagnostic tests (RDTs) for malaria show large variation in sensitivity and specificity, and there are concerns about their stability under field conditions. To improve current RDTs, monoclonal antibodies (mAbs) for novel malaria antigens have been developed and screened for their possible use in new diagnostic tests. Thre...
Vroegtijdige diagnose van malaria bij zwangere vrouwen is belangrijk om ernstige gevolgen voor moeder en kind te voorkomen. Eline Kattenberg onderzocht gepubliceerde evaluaties van diagnostische tests. Vervolgens bekeek ze verschillende aspecten van bestaande testen in Burkina Faso. Kattenberg concludeert dat alle onderzochte diagnostica - inclusie...
During pregnancy, malaria infection with Plasmodium falciparum or Plasmodium vivax is related to adverse maternal health and poor birth outcomes. Diagnosis of malaria, during pregnancy, is complicated by the absence or low parasite densities in peripheral blood. Diagnostic methods, other than microscopy, are needed for detection of placental malari...
Characteristics of included studies. All studies included in the review are listed in this table. The clinical features, study design and participants are given for each study individually. The reference and index tests are listed that were used in each study and the prevalence as measured by the reference test is reported together with the encount...
Evaluations not included in the meta-analysis. This file lists the evaluations that were not included in the meta-analysis or discussed in the review, because of the low number of studies, or use of reference test. The sensitivity and specificity and their 95% confidence intervals (CI) are listed for each comparison of index and reference test for...
The Clark electrode, which has been commercially available for more than 50 years, is a robust first-generation sensor originally used to determine the concentration of dissolved oxygen. This paper describes a simple experimental setup employing the Clark electrode to measure low concentrations of aqueous solutions of dissolved nitric oxide (NO) (>...
Projects
Project (1)
While the burden of malaria decreased in the past 2 decades, cases have now stabilized, signalling that without sustained control pressure and novel approaches, these gains could be reversed. There is an urgent need to strengthen surveillance systems to achieve successful malaria control and elimination.
Together with our partner institutes in Peru (UPCH-ITMAvH) and Vietnam (NIMPE) we aim to:
- Development of country-specific NGS tools for P. falciparum and P. vivax
- Monitor evolution and spread of drug resistance using a combination of drug resistance and population genetics markers
- Study the effect of malaria control interventions on parasite populations using NGS tools and WGS
- Support malaria surveillance as a core intervention in National Malaria Control Programs in Vietnam and Peru