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Johanna Heuveling

Johanna Heuveling

Dr. rer. nat.

About

13
Publications
1,302
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917
Citations

Publications

Publications (13)
Article
Full-text available
Localization of substrates in membrane proteins is an important but challenging task. In this paper, we show that deuterium electron spin echo envelope modulation spectroscopy (²H ESEEM) combined with site-directed spin labeling is a powerful tool to localize the substrate, histidine-d5, in the ABC transporter HisQMP2. Based on a homology model and...
Article
Canonical ATP-binding cassette (ABC) importers are major players in the translocation of numerous nutrients, vitamins, and growth factors to the cytoplasm of prokaryotes. Moreover, some ABC importers have been identified as virulence factors in bacterial pathogenesis. Thus, a full understanding of their mode of action is considered a prerequisite,...
Article
ATP-binding cassette (ABC) transport systems comprise two transmembrane domains/subunits that form a translocation path and two nucleotide-binding domains/subunits that bind and hydrolyze ATP. Prokaryotic canonical ABC import systems require an extracellular substrate-binding protein for function. Knowledge of substrate-binding sites within the tra...
Article
Full-text available
ATP-binding cassette (ABC) transporters are ubiquitous integral membrane proteins that translocate a variety of substrates, ranging from ions to macromolecules, either out of or into the cytosol (hence defined as importers or exporters, respectively). It has been demonstrated that ABC exporters and importers function through a common mechanism invo...
Article
Previously, [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD)-based fluorophores used as highly sensitive fluorescence lifetime probes reporting on their microenvironmental polarity have been described. Now, a new generation of DBD dyes has been developed. Although they are still sensitive to polarity, in contrast to the former DBD dyes, they have extraor...
Article
Prokaryotic solute binding protein-dependent ATP-binding cassette (ABC) import systems are divided into type I and type II and mechanistic differences in the transport process going along with this classification are under intensive investigation. Little is known about the conformational dynamics during the catalytic cycle especially concerning the...
Article
The type I ATP-binding cassette (ABC) importer for positively charged amino acids of the thermophilic bacterium Geobacillus stearothermophilus consists of the extracellular solute binding protein, ArtJ, and a homodimer each of the transmembrane subunit, ArtM, and the nucleotide-binding and -hydrolyzing subunit, ArtP. We have investigated the functi...
Article
ATP binding cassette (ABC) transporters can be found in all organism form bacteria to man. Dysfunction of these transporters is often associated with diseases, like multi drug-resistance in cancer cells. These transporters translocate substances across the cellular plasma membrane under the expense of ATP, consist of dimers of ATPase subunits known...
Article
Lon protease is a major protease in cellular protein quality control, but also plays an important regulatory role by degrading various naturally unstable regulators. Here, we traced additional such regulators by identifying regulons with co-ordinately altered expression in a lon mutant by genome-wide transcriptional profiling. Besides many members...
Article
Full-text available
The σS (or RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli. While nearly absent in rapidly growing cells, σS is strongly induced during entry into stationary phase and/or many other stress conditions and is essential for the expression of multiple stress resistances. Genome-wide expression...

Questions

Question (1)
Question
Wir sind eine Gruppe aus dem Masterstudiengang Wissenschaftsmarketing an der TU Berlin und wir sammeln Meinungen für eine Recherche.
Wenn Du irgendwelche MINTler kennst, dann schicke ihnen bitte diesen Fragebogen weiter.
Oder wenn Du selbst einer bist, dann nimm Dir bitte 4 Minuten Zeit für die Fragen.
Vielen Dank!
Johanna Heuveling

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