Johan Sanders

University of Groningen | RUG · Engineering and Technology institute Groningen (ENTEG)

As the global population rises, agriculture and industry are under increasing pressure to become more sustainable in meeting this growing demand, while minimizing impacts on global emissions, land use change, and biodiversity. The development of efficient and symbiotic local bio-economies can help to respond to this challenge by using land, resourc...

The population of the world is ever-growing. It is expected to reach 10 billion in 2050. To feed all those people, significant improvements in our food production systems are required. Current agricultural practices have proven to be damaging to ecological systems worldwide, leading to greenhouse gas emissions, deforestation, loss of biodiversity a...

Grasses, legumes and green plant wastes represent a ubiquitous feedstock for developing a bioeconomy in regions across Europe. These feedstocks are often an important source of ruminant feed, although much remains unused or underutilised. In addition to proteins, these materials are rich in fibres, sugars, minerals and other components that could a...

The objective of this study is to investigate the effect of replacing grass silage with biorefined grass silage (press cake silage) on dry matter intake (DMI), milk production and composition, rumen fermentation parameters, nitrogen and phosphorus excretion of early lactation Holstein Friesian dairy cows. An in vitro experiment using the rumen simu...

Global warming and climate change are imminent threats to the future of humankind. A shift from the current reliance on fossil fuels to renewable energy is key to mitigating the impacts of climate change. Biological raw materials and residues can play a key role in this transition through technologies such as anaerobic digestion. However, biologica...

Abstract: Perennial rye grass is a widely used forage species in Ireland, on which the ruminant sector of agriculture is heavily dependent. While this species of grass is the primary source of fodder for cows, it is also abundant in plant protein, which could form a potential alternative ingredient in monogastric animal feed using a green biorefine...

The cover image is based on the Review Article The protein challenge: Matching future demand and supply in Indonesia by Widyarani et al., https://doi.org/10.1002/bbb.2176. The cover image is based on the Review Article The protein challenge: Matching future demand and supply in Indonesia by Widyarani et al., https://doi.org/10.1002/bbb.2176.

Indonesia has the fourth largest population in the world and in the coming years food production will need to catch up with its growth. To fulfill the protein demand of this growing population, the productivity of the Indonesian agricultural sector should be increased. This can be achieved either by expanding the agricultural land or by increasing...

The population of the world is ever-growing. It is expected to reach 10 billion in 2050. To feed all those people, significant improvements in our food production systems are required. Current agricultural practices have proven damaging to ecological systems worldwide, leading to greenhouse gas emissions, deforestation, loss of biodiversity and wat...

p>Method for separating amino and/or organic acid-containing particles from a mixture of amino and/or organic acid-containing particles based on density, comprising providing a feed of the amino and/or organic acid-containing particles providing a medium having a selected density effective for particles of a lower density than the selected density...

Primary processing of oil-containing material involves pre-treatment processes, oil recovery processes and the extraction and valorisation of valuable compounds from waste streams. Pre-treatment processes, e.g. thermal, enzymatic, electrical and radio frequency, have an important effect on the oil recovery, quality of oil, extraction possibility an...

This chapter discusses biochemical methods to locate genes on the physical map of yeast mitochondrial DNA. The mtDNA of the yeasts Saccharomyces carlsbergensis and Saccharomyces cerevisiae is a circular duplex DNA with a molecular weight of about 50 x 106. Specific fragments of this DNA have been made with restriction endonucleases and the fragment...

1 We have used restriction enzyme analysis of petite mtDNAs to construct a detailed physical map of the 21S region on the mtDNA of the Saccharomyces cerevisiae strain JS1-3D. The map covers a segment of about 20,000 bp, on which the recognition sites of the enzymes HapII, HindII, HindIII, SalI, XhoI and HhaI have been localized (22 sites in total)....

1) We have constructed independent physical maps of the mtDNAs from three different wild-type Saccharomyces strains by double-digestion analysis and hybridization analysis, using restriction endonucleases EcoRI, HindII, HindIII, PstI, BamHI, Aval, HhaI, SalI and XhoI. Twentynine restriction enzyme sites have been localized on the mtDNA of Saccharom...

We have fractionated fragments of yeast mtDNA, obtained with restriction endonucleases, on poly(U)-Sephadex columns using the procedure of Flavell and Van den Berg (FEBS Letters (1975) 58, 90–93). The poly(U) forms a triple helix with (dA·dT) clusters in duplex DNA and fractionates DNA fragments on the basis of the length and number of clusters con...

1. We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels. 2. Endonucleases EcoRI, HindII + III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and greater than 80 fragments, respectively. 3. By ana...

We have determined the position of the two rRNA cistrons on the physical map of the mtDNA of Saccharomyces carlsbergensis obtained with restriction endonucleases. Hybridization of 125I-labelled rRNA with DNA fragments of known location on the map shows that the two rRNA cistrons are at least 25 000 base pairs apart on this DNA of 70 000 base pairs.

Long DNA can be recovered from agarose gels after electrophoresis by freezing the gel slices and manually squeezing out liquid containing the DNA. With this method the recoveries of phage T7 DNA (molecular weight 25 × 106) and the open and closed forms of circular phage PM2 DNA (molecular weight 6 × 106) were about 70%. Sedimentation analysis shows...

1. Both nuclear and mtDNA of four different yeasts show approximately 10% homology as measured by DNA · DNA filter hybridization. These homologous sequences are mainly attributable to the ribosomal cistrons.2. Melting curve analysis shows that the heterologous mitochondrial DNA · DNA hybrids contain several times more mismatching than the nuclear D...

We have determined the genetic complexity of mtDNA from Euglena gracilis, var. bacillaris, by DNA renaturation experiments. Renaturation was followed by the specific digestion of single-stranded DNA with nuclease S1. The complexity found with this method is about 40 · 106, i.e. in the range reported for some other unicellular eukaryotes. The molecu...

1. We have isolated a closed circular DNA fraction from mitochondria purified from Kluyveromyces lactis by centrifuging a mitochondrial lysate to equilibrium in CsCl containing ethidium bromide. Electron micrographs of appropriate gradient fractions show predominantly circular duplex DNA with an average contour length () of . The circular DNA has t...

1. We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels. 2. Endonucleases EcoRI, HindII+III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and >80 fragments, respectively. 3. By analysis of parti...

1. The kinetics of DNA‐DNA filter hybridization have been studied using model systems. The results suggest that two limiting reactions occur. a) When the hybridization reaction is fast ( i.e. with a DNA of low complexity such as phage T7 at ⋝ 5 μg DNA per filter) the reaction becomes controlled by the diffusion of DNA to the filter. This is shown b...

1.1. We have previously shown that the mtDNA of the ethidium-induced cytoplasmic petite mutant RD1A of the yeast Saccharomyces cerevisiae consists of perfect tandem repetitions of a very sequence of < 300 base pairs.2.2. The complementary strands of RD1A mtDNA were separated in alkaline CsCl and used for DNA · DNA hybridization with wild-type mtDNA...