Jörg Schnauß

Jörg Schnauß
University of Leipzig · Peter Debye Institute

Dr. rer. nat.

About

54
Publications
8,510
Reads
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644
Citations
Additional affiliations
August 2020 - present
University of the West of England, Bristol
Position
  • Scientist
July 2017 - present
Fraunhofer Institute for Cell Therapy and Immunology IZI
Position
  • Scientist
Education
September 2015 - August 2018
University of Leipzig
Field of study
  • Physics - Soft Matter & Biophysics
January 2011 - September 2015
University of Leipzig
Field of study
  • Physics - Soft Matter & Biophysics
October 2004 - October 2009
University of Leipzig
Field of study
  • Physics

Publications

Publications (54)
Article
Full-text available
Despite their overwhelming complexity, living cells display a high degree of internal mechanical and functional organization which can largely be attributed to the intracellular biopolymer scaffold, the cytoskeleton. Being a very complex system far from thermodynamic equilibrium, the cytoskeleton’s ability to organize is at the same time challengin...
Article
Full-text available
Attractive depletion forces between rodlike particles in highly crowded environments have been shown through recent modeling and experimental approaches to induce different structural and dynamic signatures depending on relative orientation between rods. For example, it has been demonstrated that the axial attraction between two parallel rods yield...
Article
Actin networks are adaptive materials enabling dynamic and static functions of living cells. A central element for tuning their underlying structural and mechanical properties is the ability to reversibly connect, i.e., transiently crosslink, filaments within the networks. Natural crosslinkers, however, vary across many parameters. Therefore, syste...
Article
Full-text available
Solvent conditions are unexpectedly sufficient to drastically and reversibly slow down cells. In vitro on the molecular level, protein–solvent interactions drastically change in the presence of heavy water (D2O) and its stronger hydrogen bonds. Adding D2O to the cell medium of living cells increases the molecular intracellular viscosity. While cell...
Article
Full-text available
Semiflexible polymers form central structures in biological material. Modelling approaches usually neglect influences of polymer-specific molecular features aiming to describe semiflexible polymers universally. Here, we investigate the influence of molecular details on networks assembled from filamentous actin, intermediate filaments, and synthetic...
Article
Full-text available
Biopolymer networks play a major role as part of the cytoskeleton. They provide stable structures and act as a medium for signal transport. These features encourage the application of such networks as organic computation devices. While research on this topic is not advanced yet, previous results are very promising. The protein actin in particular a...
Article
Full-text available
Entangled semiflexible polymer networks are usually described by the tube model, although this concept has not been able to explain all experimental observations. One of its major shortcomings is neglecting the thermal fluctuations of the polymers surrounding the examined test filament, such that disentanglement effects are not captured. In this st...
Article
Full-text available
Networks of crosslinked keratin filaments are abundant in epithelial cells and tissues, providing resilience against mechanical forces and ensuring cellular integrity. Although studies of in vitro models of reconstituted keratin networks have revealed important mechanical aspects, the mechanical properties of crosslinked keratin structures remain p...
Article
Full-text available
Cell contractility is mainly imagined as a force dipole-like interaction based on actin stress fibers that pull on cellular adhesion sites. Here, we present a different type of contractility based on isotropic contractions within the actomyosin cortex. Measuring mechanosensitive cortical contractility of suspended cells among various cell lines all...
Preprint
Full-text available
Established model theories, developed to capture the mechanical behavior of soft complex materials composed of semiflexible polymers assume entropic interactions between filaments to determine the mechanical response. In recent studies, the general accepted tube model has been challenged in terms of its basic assumption about filament-filament inte...
Article
Full-text available
In article number 2101840, Jörg Schnauß and co‐workers show that heavy water (D2O) reversibly slows down cells, retards cell invasion, and drastically impacts cell mechanics, possibly prolonging storage times for biological materials. The cellular dynamics, which transform into slow motion, can be captured via the time‐temperature superposition pri...
Article
Full-text available
Single-filament tracing has been a valuable tool to directly determine geometrical and mechanical properties of entangled polymer networks. However, systematically verifying how the stiffness of the tracer filament or its molecular interactions with the surrounding network impacts the measurement of these parameters has not been possible with the e...
Article
Full-text available
The cytoskeleton is a major determinant of cell mechanics, and alterations in the central mechanical aspects of cells are observed during many pathological situations. Therefore, it is essential to investigate the interplay between the main filament systems of the cytoskeleton in the form of composite networks. Here, we investigate the role of kera...
Preprint
The cytoskeleton is a major determinant of cell mechanics, a property that is altered during many pathological situations. To understand these alterations, it is essential to investigate the interplay between the main filament systems of the cytoskeleton in the form of composite networks. Here, we investigate the role of keratin intermediate filame...
Preprint
Full-text available
Single-filament tracing has been a valuable tool to directly determine geometrical and mechanical properties of entangled polymer networks. However, systematically verifying how the stiffness of the tracer filament or its molecular interactions with the surrounding network impacts the measurement of these parameters has not been possible with the e...
Article
Der vorliegende Beitrag beleuchtet als Teil eines Blended-Learning Ansatzes vorrangig den Einsatz von Live-Umfragen (ARS – Audience Responce Systems) im Vorlesungsrahmen. Gerade naturwissenschaftlich geprägte Studiengänge (hier die Fachrichtung Physik) sind häufig durch Frontalunterricht geprägt. Das maßgebliche Ziel des Projektes war es das Format...
Article
Filaments of the cellular protein actin can form bundles, which can conduct ionic currents as well as mechanical and voltage solitons. These inherent properties can be utilized to generate computing circuits solely based on self-assembled actin bundle structures. Starting with experimentally observed networks of actin bundles, we model their networ...
Article
Full-text available
Melanoma cells are often surrounded by hyaluronic acid (HA) rich environments, which are considered to promote tumor progression and metastasis. Induced effects in compound materials consisting of cells embedded in an extracellular matrix (ECM) have been studied, however, alterations of the single cells have never been addressed. Here, we explicitl...
Preprint
Full-text available
The innate immune response triggered by CpG DNA can improve host survival following pathogen challenge. Whether CpG ODN-mediated immune activation leads to global molecular changes in cells that are detectable by FTIR spectroscopy is currently unknown. Here, we used Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) spectroscopy t...
Preprint
ATR-FTIR Spectroscopy of aqueous cell culture samples is explained step-by-step. Infrared spectra acquisition, processing and analysis is included briefly.
Preprint
ATR-FTIR Spectroscopy of aqueous cell culture samples is explained step-by-step. Infrared spectra acquisition, processing and analysis is included briefly.
Article
Full-text available
The actin droplet machine is a computer model of a three-dimensional network of actin bundles developed in a droplet of a physiological solution, which implements mappings of sets of binary strings. The actin bundle network is conductive to travelling excitations, i.e. impulses. The machine is interfaced with an arbitrary selected set of k electrod...
Preprint
Full-text available
Starting with an experimentally observed networks of actin bundles, we model their network structure in terms of edges and nodes. We then compute and discuss the main electrical parameters, considering the bundles as electrical wires. A set of equations describing the network is solved with several initial conditions. Input voltages, that can be co...
Article
Full-text available
Actin filaments are conductive to ionic currents, mechanical and voltage solitons. These travelling localisations can be utilised to generate computing circuits from actin networks. The propagation of localisations on a single actin filament is experimentally unfeasible to control. Therefore, we consider excitation waves propagating on bundles of a...
Article
Full-text available
Correction for ‘The role of stickiness in the rheology of semiflexible polymers’ by Tom Golde et al. , Soft Matter , 2019, 15 , 4865–4872.
Preprint
Full-text available
The actin droplet machine is a computer model of a three-dimensional network of actin bundles developed in a droplet of a physiological solution, which implements mappings of sets of binary strings. The actin bundle network is conductive to travelling excitations, i.e. impulses. The machine is interfaced with an arbitrary selected set of k electrod...
Preprint
Full-text available
Actin filaments are conductive to ionic currents, mechanical and voltage solitons. These travelling localisations can be utilised in making the actin network executing specific computing circuits. The propagation of localisations on a single actin filament is experimentally unfeasible, therefore we propose a `relaxed' version of the computing on ac...
Chapter
Full-text available
Actin and tubulin are key structural elements of Eukaryotes’ cytoskeleton. The networks of actin filaments and tubulin microtubules are substrates for cells’ motility and mechanics, intra-cellular transport and cell-level learning. Ideas of information processing taking place on a cytoskeleton network, especially in neurons, have been proposed by S...
Preprint
Full-text available
Semiflexible polymers form central structures in biological material. Modeling approaches usually neglect influences of polymer-specific molecular features aiming to describe semiflexible polymers universally. Here, we investigate the influence of molecular details on networks assembled from filamentous actin, intermediate filaments, and synthetic...
Preprint
Full-text available
We propose a road-map to experimental implementation of cytoskeleton-based computing devices. An overall concept is described in the following. Collision-based cytoskeleton computers implement logical gates via interactions between travelling localisation (voltage solitons on AF/MT chains and AF/MT polymerisation wave fronts). Cytoskeleton networks...
Article
Full-text available
The cytoskeleton is a highly interconnected meshwork of strongly coupled subsystems providing mechanical stability as well as dynamic functions to cells. To elucidate the underlying biophysical principles, it is central to investigate not only one distinct functional subsystem but rather their interplay as composite biopolymeric structures. Two of...
Article
Oral squamous cell carcinomas (OSCC) are the sixth most common cancer and the diagnosis is often belated for a curative treatment. The reliable and early differentiation between healthy and diseased cells is the main aim of this study in order to improve the quality of the treatment and to understand tumour pathogenesis. Here, the optical stretcher...
Chapter
Cells display a high degree of functional organization, largely attributed to the intracellular biopolymer scaffold known as the cytoskeleton. This inherently complex structure drives the system out of equilibrium by constantly consuming energy to conserve or reorganize its structure. Thus, the active, structurally organized cytoskeleton is the key...
Chapter
In biotechnology and medicine, controlled studies on biological material are fundamental for developing new methodologies and therapeutic approaches. To explore the nature of biological processes and to test inherent material properties, it has become increasingly clear that new experimental methods must be developed in order to allow precise manip...
Article
Mechanical properties of complex, polymer-based soft matter, such as cells or biopolymer networks, can be understood in neither the classical frame of flexible polymers nor of rigid rods. Underlying filaments remain outstretched due to their non-vanishing backbone stiffness, which is quantified via the persistence length (lp), but they are also sub...
Article
Full-text available
Bundled actin structures play an essential role in the mechanical response of the actin cytoskeleton in eukaryotic cells. Although responsible for crucial cellular processes, they are rarely investigated in comparison to single filaments and isotropic networks. Presenting a highly anisotropic structure, the determination of the mechanical propertie...
Article
Full-text available
Cytoskeletal filaments provide cells with mechanical stability and organization. The main key players are actin filaments and microtubules governing a cell's response to mechanical stimuli. We investigated the specific influences of these crucial components by deforming MCF-7 epithelial cells at small (≤5% deformation) and large strains (>5% deform...
Article
Rheology of living cells has developed an increasing need for high throughput measurements. Diseases such as cancer heavily remodel the cytoskeleton and impinge on cellular functions. Cells affected by such diseases show altered rheologic responses on many different levels rendering cells' mechanical fingerprints—a potential target for diagnostics....
Article
Full-text available
The mechanics of complex soft matter often cannot be understood in the classical physical frame of flexible polymers or rigid rods. The underlying constituents are semiflexible polymers, whose finite bending stiffness ($\kappa$) leads to non-trivial mechanical responses. A natural model for such polymers is the protein actin. Experimental studies o...
Article
Full-text available
Bundles and networks of semiflexible biopolymers are key elements in cells, lending them mechanical integrity while also enabling dynamic functions. Networks have been the subject of many studies, revealing a variety of fundamental characteristics often determined via bulk measurements. Although bundles are equally important in biological systems,...
Article
Full-text available
The self-assembly of molecular and macromolecular building blocks into organized patterns is a complex process found in diverse systems over a wide range of size and time scales. The formation of star- or aster-like configurations, for example, is a common characteristic in solutions of polymers or other molecules containing multi-scaled, hierarchi...
Article
Full-text available
A central part of soft matter physics is the investigation of effects in an active environment. These systems are driven out of equilibrium by a constant energy consumption. In biological systems, for instance, energy is consumed in the dynamic polymerization process of cytoskeletal filaments or by motor-filament interactions. These active processe...
Article
Full-text available
Biopolymer networks contribute mechanical integrity as well as functional organization to living cells. One of their major constituents, the protein actin, is present in a large variety of different network architectures, ranging from extensive networks to densely packed bundles. The shape of the network is directly linked to its mechanical propert...
Article
Full-text available
Biopolymer networks contribute mechanical integrity as well as functional organization to living cells. One of their major constituents, the protein actin, is present in a large variety of different network architectures, ranging from extensive networks to densely packed bundles. The shape of the network is directly linked to its mechanical propert...
Article
Full-text available
Modeling approaches of suspended, rod-like particles and recent experimental data have shown that depletion forces display different signatures depending on the orientation of these particles. It has been shown that axial attraction of two rods yields contractile forces of 0.1pN that are independent of the relative axial shift of the two rods. Here...
Preprint
Full-text available
Modeling approaches of suspended, rod-like particles and recent experimental data have shown that depletion forces display different signatures depending on the orientation of these particles. It has been shown that axial attraction of two rods yields contractile forces of 0.1 pN that are independent of the relative axial shift of the two rods. Her...
Article
Full-text available
We studied the influence of fluorescent polystyrene beads on both entangled and cross-linked actin networks. Thermal bead fluctuations were observed via video particle tracking and analyzed with one-point microrheology. Illumination of fluorescent beads with their appropriate excitation wavelength leads to a drastic softening of actin gels. Other w...
Article
Full-text available
F-actin bundles are prominent cytoskeletal structures in eukaryotes. They provide mechanical stability in stereocilia, microvilli, filopodia, stress fibers and the sperm acrosome. Bundles are typically stabilized by a wide range of specific crosslinking proteins, most of which exhibit off-rates on the order of 1s(-1). Yet F-actin bundles exhibit st...

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