Job Alves de Souza Fh- Philosophiæ Doctor
- Ezequiel Dias Foundation
Job Alves de Souza Fh
- Philosophiæ Doctor
- Ezequiel Dias Foundation
About
16
Publications
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211
Citations
Introduction
Current institution
Additional affiliations
February 2012 - present
Publications
Publications (16)
Ensuring accuracy in the diagnosis of leishmaniasis is crucial due to the myriad of potential differential diagnoses. Given the inherent limitations of serological techniques, real-time polymerase chain reaction (qPCR) emerges as a superior alternative. Furthermore, parasitological methods, conventionally regarded as the gold standard owing to thei...
Diagnostic networks ensure efficiency in disease diagnosis. A descriptive study evaluated the network of public health laboratories (NPHL) in Minas Gerais State, Brazil, using diagnostic results for tegumentary leishmaniasis (TL) from the laboratory management system in 2017–2020. Out of 1,369 individuals analyzed, 704 (51.4%) cases of TL were conf...
Visceral leishmaniasis (VL) requires diagnostic assays to complement clinical suspicion. However, there is no standardization of a diagnostic flow using available assays. This study aimed to evaluate the performance of parasitological, molecular, and serological assays for diagnosing VL and propose a diagnostic flow based on performance, practicali...
Objective
To assess the performance of the proposed new diagnostic pathway in patients with suggestive clinical symptoms of CL within MG's public laboratory network.
Methods
This is a descriptive study where the results of CL tests were analyzed in patients with clinical suspicion of the disease who had their tests conducted within MG's public lab...
Introduction: Visceral leishmaniasis (VL) requires diagnostic assays to complement clinical suspicion. However, there is no standardization of a diagnostic flow using available assays. This study aims to evaluate the performance of parasitological, molecular, and serological assays for diagnosing VL and propose a diagnostic flow based on performanc...
Diagnosis of Trypanosoma cruzi (T. cruzi) infection in the chronic phase of Chagas disease (CD) is performed by serologic testing. Conventional tests are currently used with very good results but require time, laboratory infrastructure, and expertise. Rapid diagnostic tests (RDTs) are an alternative as the results are immediate and do not require s...
Visceral leishmaniasis (VL) is a vector-borne disease that is caused by the protozoan Leishmania infantum and transmitted to humans and other mammals through the bites of infected female sandflies. VL has been prevalent in Minas Gerais, Brazil, since the 1940s. As the vector completes its reproductive cycle in the soil with organic matter, the asso...
Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, includ...
The applicability of molecular biology/PCR for canine visceral leishmaniasis diagnosis presents challenges, mainly due to the diversity of targets described. The objectives of this study were to compare the sensitivities and reliability of five targets (kDNA/120, kDNA/145, ITS1, hsp70/234 and hsp70/1300) in four different tissue samples (bone marro...
Introduction:
Leishmania infantum was considered to be absent from Amapá until 2017 when canine infection was detected. However, there is a lack of knowledge about which reservoir species are involved in transmission in this region.
Methods:
Between 2014 and 2016, 86 samples from wild mammals and 74 from domestic dogs were collected in Wajãpi In...
Hepatitis delta virus (HDV) has been associated with acute or chronic hepatitis in Latin America, but there is no prevalence study covering South American countries. This meta-analysis aimed to estimate anti-HDV prevalence through a systematic review of published articles in English, Portuguese and Spanish until December 2017. Searches were conduct...
The diagnosis and control of canine visceral leishmaniasis (CVL) has fundamental importance in the control of human visceral leishmaniasis. In this context, the immunochromathographic test (ICT) has emerged as a valuable diagnostic tool. Currently, in Brazil, the algorithm for the diagnosis of CVL uses Dual Path Platform (DPP) ICT as a screening te...
Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understand this host-pathogen interplay....
Brucella, the etiological agent of animal and human brucellosis, is a bacterium with the capacity to modulate the inflammatory response. Cyclic β-1,2-glucan (CβG) is a virulence factor key for the pathogenesis of Brucella as it is involved in the intracellular life cycle of the bacteria. Using comparative studies with different CβG mutants of Bruce...
Subinhibitory concentrations (SICs) of antimicrobials may result in alterations in bacterial biology with implications for its potential aggression. This has considerable importance for the resident microbiota. Our aim was to analyze the effects of SICs of antimicrobials on the morphological, biochemical, physiological and molecular characteristics...
The use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. We aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. Selective cultures for enterobacteria (ENT), non-fermenting Gram-negative rods (NFR) and Gram-po...
Questions
Questions (5)
My hypothesis is that vaccination in infected/hospitalized patients with worsening of covid-19 could cause an increase in the levels of neutralizing antibodies, improving the prognosis and preventing deaths. Even with the bias of treatments with immunosuppressants (anti-inflammatory drugs, for example), the activation of the production of these neutralizing antibodies could be faster since patients have already had previous contact with the virus. In addition, studies show that individuals with long term covid-19 improve symptoms after vaccination. So, why not try this strategy?
Ion-exchange Chromatography (IEC) and Size Exclusion Chromatography (SEC) method or PEG precipitation followed by sucrose density gradient?
For use as an antigen, for example, it would be important to separate viral particles from other proteins and keep the protein structure as intact as possible.
Dear colleagues
I found a trypomastigote of Trypanosoma cruzi in the bone marrow aspirate from a patient. Although the sample is very characteristic of marrow, I'm afraid that may have occurred some contamination with peripheral blood. It is possible that this finding is truthful?
Between RPMI and Schneider what is the best?
My purpose would be to isolate Leishmania infantum from dogs.
In a culture of Bone Marrow Derived Macrophage (BMDM) the supplementation of the medium with 10% of Bovine Fetal Serum (BFS) inhibits the detection of IL-1β by ELISA, but not of other cytokines.
This problem can be solved by supplementing the medium with just 1% of BFS. Evidence suggests that the IL1β production is not inhibited, but rather the release.
Does anyone have an explanation for why this occurs? What is the mechanism of inhibition of IL-1β by BFS?
