Joaquim Miguel Oliveira

Joaquim Miguel Oliveira
University of Minho · 3B's Research Group in Biomaterials, Biodegradables and Biomimetics

BSc, PhD

About

652
Publications
107,597
Reads
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10,822
Citations
Introduction
Prof. Miguel Oliveira is one of the founding Editors in chief of In vitro Models Journal (Springer Nature). See: https://www.springer.com/journal/44164
Additional affiliations
July 2016 - June 2020
University of Minho
Position
  • Managing Director
June 2009 - August 2016
University of Minho
Position
  • Assistant Researcher, Pro-Director
Description
  • http://www.3bs.uminho.pt/users/migueloliveira
May 2006 - December 2007
National Institute of Advanced Industrial Science and Technology
Position
  • Invited PhD student

Publications

Publications (652)
Article
Spinal cord injury (SCI) is an intractable condition with complex pathological processes and poor prognosis. Reactive oxygen species (ROS) generation induced by the mammalian target of the rapamycin (mTOR) protein is one of the causes of secondary inflammation of SCI. Rapamycin (Rapa) is a pharmacological inhibitor of mTOR, which can inhibit ROS ov...
Article
Full-text available
Osteoporosis is defined by loss of bone mass and deteriorated bone microarchitecture. The present study compared the effects of available pharmacological and non-pharmacological agents for osteoporosis [alendronate (ALE) and concomitant supplementation of vitamin D (VD) and calcium (Ca)] with the effects of bovine colostrum (BC) supplementation in...
Article
Hydrogels are a recurrent platform for Tissue Engineering (TE) strategies. Their versatility and the variety of available methods for tuning their properties highly contribute to hydrogels’ success. As a result, the design of advanced hydrogels has been thoroughly studied, in the quest for better solutions not only for drugs‐ and cell‐based therapi...
Article
An important hallmark in cancer research is the discovery of suitable features capable to reliably predict tumor invasiveness, and consequently, their metastatic potential at an early stage. Current methods are based on molecular biomarker screening and imaging that may not reveal the altered properties of tumor cells, being also labor-intensive an...
Article
Full-text available
Marine origin polymers represent a sustainable and natural alternative to mammal counterparts regarding the biomedical application due to their similarities with proteins and polysaccharides present in extracellular matrix (ECM) in humans and can reduce the risks associated with zoonosis and overcoming social- and religious-related constraints. In...
Article
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In the field of tissue engineering and regenerative medicine (TERM), the use of traditional biomaterials capable of integrating the host tissue to promote the healing and regenerative process while it degrades has become less and less a focus of inspiration [...]
Article
Purpose: The aim of this study was to evaluate the clinical outcome at 5-year follow-up of a one-step procedure combining anterior cruciate ligament (ACL) reconstruction and partial meniscus replacement using a polyurethane scaffold for the treatment of symptomatic patients with previously failed ACL reconstruction and partial medial meniscectomy....
Article
Full-text available
Anterior cruciate ligament (ACL) replacement is still a big challenge in orthopedics due to the need to develop bioinspired implants that can mimic the complexity of bone-ligament interface. In this study, we propose biomimetic composite tubular grafts (CTGs) made of horseradish peroxidase (HRP)-cross-linked silk fibroin (SF) hydrogels containing Z...
Article
Osteoarthritis (OA) is a progressive degenerative disease that causes severe pain and functional limitation, especially during locomotion. It is the most common arthritis type that damages the surface of articular cartilage until the underlying bone. In the past decade, the scientific community has made a considerable effort to improve or discover...
Article
Full-text available
Oxaliplatin is the first-line regime for advanced gastric cancer treatment, while its resistance is a major problem that leads to the failure of clinical treatments. Tumor cell heterogeneity has been considered as one of the main causes for drug resistance in cancer. In this study, the mechanism of oxaliplatin resistance was investigated through in...
Article
Stimulating brain tissue regeneration is a major challenge after central nervous system (CNS) injury, such as those observed from trauma or cerebrovascular accidents. Full regeneration is difficult even when a neurogenesis-associated repair response may occur. Currently, there are no effective treatments to stimulate brain tissue regeneration. Howe...
Article
Peripheral nerve injuries are a major source of disabilities, and treatment of long nerve gap autografts is the gold standard. However, due to poor availability and donor-site morbidity, research is directed towards the development of regenerative strategies based on the use of artificial nerve guidance conduits (NGCs). Several properties and chara...
Article
Full-text available
Cell transplantation has been studied extensively as a therapeutic strategy for neurological disorders. However, to date, its effectiveness remains unsatisfactory due to low precision and efficacy of cell delivery; poor survival of transplanted cells; and inadequate monitoring of their fate in vivo. Fortunately, different bio-scaffolds have been pr...
Article
Human tissues and organs are inherently heterogeneous, and their functionality is determined by the interplay between cell types, their secondary architecture, and gradients of signalling molecules and metabolites. To mimic the dynamics of native tissues, perfusion bioreactors and microfluidic devices are widely used, enhancing cell culture viabili...
Chapter
Articular cartilage lesions are a common pathology of the knee joint. A careful evaluation of cartilage and osteochondral lesions is essential in order to choose the most suitable treatment. This becomes more relevant in young patients, with a good overall cartilage situation. The physical examination must be done by the orthopedic surgeon that sho...
Chapter
Recent advances in tissue engineering (TE) have shown that combining biomaterials, cells, and bioactive molecules are important to promote the regeneration of damaged tissues or as therapeutic systems. Porous three-dimensional structures with interconnected pore network are capable to guide the development of functional engineered tissues and affor...
Chapter
Current strategies in the field of tissue engineering are bringing functional biomaterials with required structural, mechanical, and biological performance able to endorse the repair and regeneration of injured or diseased tissues. Biocomposites formed by biodegradable polymers matrix and bioceramics have proved their effectiveness in clinics, name...
Article
In bone tissue engineering, the development of advanced biomimetic scaffolds has led to the quest for biomotifs in scaffold design that better recreate the bone matrix structure and composition and hierarchy at different length scales. In this study, an advanced hierarchical scaffold consisting of silk fibroin combined with a decellularized cell-de...
Article
The application of nanoparticles in magnetic resonance imaging (MRI) has been greatly increasing, due to their advantageous properties such as nanoscale dimension and tuneability. In this context, manganese (Mn²⁺)-based nanoparticles have been greatly investigated, due to their valuable use as a contrast agent, improving signal intensity and specif...
Article
The simultaneous generation of multiple tissues and their functional assembly into complex tissues remains a critical challenge for regenerative medicine. The tissue-to-tissue interface connecting two adjacent tissues is vital in effective tissue function. The presented worked hypothesize that differential functional property can be engineered by m...
Article
Full-text available
This study investigated the osteogenic performance of new brushite cements obtained from Li⁺-doped β-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li⁺) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) and the osteogenic differentiation-related gene express...
Article
Full-text available
The increasing rate of musculoskeletal pathologies has compelled the development of improved and novel treatment strategies in order to address unmet clinical needs. Tissue engineering approaches comprising the use of scaffolds for bone regeneration have been showing to be a promising alternative to conventional bone repair/substitution approaches....
Article
Bioengineering has been revolutionizing the production of biofunctional tissues for tackling unmet clinical needs. Bioengineers have been focusing their research in biofabrication, especially 3D bioprinting, providing cutting-edge approaches and biomimetic solutions with more reliability and cost–effectiveness. However, these emerging technologies...
Article
Full-text available
Female mice (Black 6 strain) (C57BL/6) aged 6 weeks were subject to low dose streptozotocin (STZ) treatment for five consecutive days to mimic type 1 diabetes mellitus (T1DM) with insulitis. At two weeks after STZ injections, evaluation of the elevated glucose levels was used to confirm diabetes. The diabetic mice were then subject to the transplan...
Article
Bone tissue engineering approaches have evolved towards addressing the challenges of tissue mimetic requirements over the years. Different strategies have been combining scaffolds, cells, and biologically active cues using a wide range of fabrication techniques, envisioning the mimicry of bone tissue. On the one hand, biomimetic scaffold-based stra...
Article
Full-text available
The collective dynamics of cells on surfaces and interfaces poses technological and theoretical challenges in the study of morphogenesis, tissue engineering, and cancer. Different mechanisms are at play, including, cell–cell adhesion, cell motility, and proliferation. However, the relative importance of each one is elusive. Here, experiments with a...
Article
Marine polymers such as collagen, chitosan, and fucoidan can be combined to form ionic-linked hydrogel networks towards applications in tissue engineering (TE). The use of greener approaches (as determined by green metrics -E-factor), including the absence of external chemical cross-linking agents, has advantages regarding the potential cytotoxicit...
Article
Sucrose acetate isobutyrate (SAIB) is a highly viscous hydrogel and presents many challenges for processing when its application in tissue engineering is envisioned. Herein, is proposed an innovative methodology by processing SAIB with ionic liquids (ILs), combined with silk fibroin (SF), to develop SAIB/SF scaffolds. Fourier Transform Infrared ana...
Article
The scientific community has been doing significant efforts towards engineering new 3D bone models in recent years. Osteocytes are mechanosensitive cells that play significant roles in the maintenance of bone homeostasis. Currently, as far as we know, there are no 3D models that faithfully recapitulate a bone microenvironment capable of promoting t...
Article
Organoid culture is a recently developed in-vitro three-dimensional (3D) cell culture technology. It has wide applications in tissue engineering studies. However, histological analysis of organoid is quite complex and tedious for researchers. This study proposes a user-friendly, affordable and efficient method for making formalin-fixed paraffin emb...
Article
Full-text available
Osteoporosis is characterized by bone loss. The present study aims to investigate the effects of bovine colostrum (BC) on bone metabolism using ovariectomized (OVX) and orchidectomized (ORX) rat models. Twenty-seven-week-old Wistar Han rats were randomly assigned as: (1) placebo control, (2) BC supplementation dose 1 (BC1: 0.5 g/day/OVX, 1 g/day/OR...
Article
Full-text available
Oxaliplatin (OXA) resistance in the treatment of different types of cancer is an important and complex problem. The culture of tumor organoids derived from gastric cancer can help us to provide a deeper understanding of the underlying mechanisms that lead to OXA resistance. In this study, our purpose was to understand the mechanisms that lead to OX...
Article
Natural extracellular matrix governs cells providing biomechanical and biofunctional outstanding properties, despite being porous and mostly made of soft materials. Among organs, specific tissues present specialized macro-architectures. For instance, hepatic lobules present radial organization, while vascular sinusoids are branched from vertical ve...
Article
Full-text available
Rheumatoid Arthritis (RA) is an incurable autoimmune disease that promotes the chronic impairment of patients’ mobility. For this reason, it is vital to develop therapies that target early inflammatory symptoms and act before permanent articular damage. The present study offers two novel therapies based in advanced drug delivery systems for RA trea...
Article
Full-text available
Cell therapy is a promising tool for treating central nervous system (CNS) disorders; though, the translational efforts are plagued by ineffective delivery methods. Due to the large contact surface with CNS and relatively easy access, the intrathecal route of administration is attractive in extensive or global diseases such as stroke or amyotrophic...
Article
Full-text available
The bone is a complex and dynamic structure subjected to constant stress and remodeling. Due to the worldwide incidence of bone disorders, tissue scaffolds and engineered bone tissues have emerged as solutions for bone grafting, which require sophisticated scaffolding architectures while keeping high mechanical performance. However, the conjugation...
Article
The generation of invasive fluctuating protrusions is a distinctive feature of tumor dissemination. During the invasion, individual cancer cells modulate the morphodynamics of protrusions to optimize their migration efficiency. However, it remains unclear how protrusion fluctuations govern the invasion of more complex multi-cellular structures, suc...
Article
Full-text available
Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disorder that mostly affects the synovial joints and can promote both cartilage and bone tissue destruction. Several conservative treatments are available to relieve pain and control the inflammation; however, traditional drugs administration are not fully effective and present sev...
Article
Full-text available
Chemotherapeutic resistance is a major problem in effective cancer treatment. Cancer cells engage various cells or mechanisms to resist anti-cancer therapeutics, which results in metastasis and the recurrence of disease. Considering the cellular heterogeneity of cancer stroma, the involvement of stem cells is reported to affect the proliferation an...
Article
Spinal cord injury (SCI) is a severe refractory disabling condition accompanied by complicated pathological processes, such as excessive glutamate release in the extracellular environment and massive influx of Ca²⁺ into cells. Dextran can absorb extracellular glutamate and Ca²⁺ to attenuate persistent excitatory processes that are detrimental to no...
Article
Rheumatoid arthritis (RA) is an autoimmune and chronic inflammatory disease characterized by joint inflammation. Since the inflammatory condition plays an important role in the disease process, it is important to develop and test new therapeutic approaches that specifically target and treat joint inflammation. In this study, a human 3D inflammatory...
Article
Full-text available
Bone tissue engineering (BTE) is a rapidly growing field aiming to create a biofunctional tissue that can integrate and degrade in vivo to treat diseased or damaged tissue. It has become evident that scaffold fabrication techniques are very important in dictating the final structural, mechanical properties, and biological response of the implanted...
Article
Full-text available
Hydrogels are three-dimensional platforms that serve as substitutes for native extracellular matrix. These materials are starting to play important roles in regenerative medicine because of their similarities to native matrix in water content and flexibility. It would be very advantagoues for researchers to be able to regulate cell behavior and fat...
Chapter
Chronic anterolateral ankle instability (CLAI) is a frequent pathology which might lead to incapacitating ankle dysfunction and/or pain either in athletes or general population. If neglected, CLAI can lead to late sequelae, such as impingement, cartilage damage, ankle arthritis, or deformities.
Article
The osteochondral (OC) tissue is one of the most hierarchical and complex structures known and it is composed by two main compartments of hyaline articular cartilage and subchondral bone. It exhibits unique cellular and molecular transitions from the cartilage to the bone layers. OC diseases such as osteoarthritis and traumatic lesions may affect t...
Article
Full-text available
Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed...
Article
This review focuses on vascularization and strategies involved in its evaluation and modulation. Clinical issues associated with engineered tissues of an atomically relevant size that require a vascular network to supply their cells with nutrients and oxygen are analyzed in terms of vascular network formation within scaffolds, which can be produced...
Article
Front Cover: This picture shows the in situ anti‐tumor therapeutic features of the developed calcium‐containing silk fibroin hydrogels cross‐linked in the presence of calcium peroxide and horseradish peroxidase. These are characterized by the fast gelation, compacted porous microstructure and slow drug release profile. They induced the death of enc...
Preprint
Full-text available
The collective dynamics of cells on surfaces and interfaces poses technological and theoretical challenges in the study of morphogenesis, tissue engineering, and cancer. Different mechanisms are at play, including, cell-cell adhesion, cell motility, and proliferation. However, the relative importance of each one is elusive. Here, experiments with a...
Article
In recent years, three-dimensional (3D) bioprinting has attracted wide research interest in biomedical engineering and clinical applications. This technology allows for unparalleled architecture control, adaptability and repeatability that can overcome the limits of conventional biofabrication techniques. Along with the emergence of a variety of 3D...
Article
Hydrogels, being capable of mimicking the extracellular matrix composition of tissues, are greatly used as artificial matrices in tissue engineering applications. In this study, the generation of horseradish peroxidase (HRP)‐crosslinked silk fibroin (SF) hydrogels, using calcium peroxide as oxidizer is reported. The proposed fast forming calcium‐co...
Article
Full-text available
Decades of research in orthopaedics has culminated in the quest for formidable yet resorbable biomaterials using bioactive materials. Brushite cements most salient features embrace high biocompatibility, bioresorbability, osteoconductivity, self-setting characteristics, handling, and injectability properties. Such type of materials is also effectiv...
Article
Aim: To established a simple, controlled and reproducible method to synthesize gallium (Ga)-coated polydopamine (PDA) nanoparticles (NPs). Materials & methods: PDA NPs were synthesized in alkali medium with posterior Ga shell formation due to ion chelation on the NP surface. Results: The obtained results with energy-dispersive x-ray spectroscopy co...