João Mesquita

João Mesquita
University of Porto | UP · Instituto de Ciências Biomédicas Abel Salazar (ICBAS)

DVM, MSc, PhD, Dipl. ECVM

About

193
Publications
17,871
Reads
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1,299
Citations
Additional affiliations
March 2019 - present
University of Porto
Position
  • Professor (Assistant)
January 2018 - present
University of Porto
Position
  • Researcher
Description
  • http://ispup.up.pt/research/epiunit/description/
January 2015 - December 2017
University of Porto
Position
  • Researcher
Description
  • https://cibio.up.pt/research-groups-1/details/geneasp/info

Publications

Publications (193)
Article
This study evaluated the expression of vimentin and Ki-67 proliferative index (PI) by immunohistochemistry in 30 canine gastric carcinomas (GCs) and a possible association with clinical and pathological features and patient’s survival time. Vimentin immunoreactivity was assessed in neoplastic cells (in primary lesions, emboli, and metastases) and t...
Article
Rickettsiosis is considered an emerging/re-emerging vector-borne disease that causes significant public health threats. Ticks are reservoirs and vectors of Rickettsia having a significant role in the transmission of rickettsiae. In Portugal, little is known about tick-borne Rickettsia species in sheep. Therefore, this study aimed to investigate ric...
Article
Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread zoonotic pathogen that can cause mild to severe hemorrhagic disease in humans. CCHFV may be transmitted through direct contact with tissue or blood of viremic animals; however, the primary transmission route is through infected tick bites. CCHFV RNA has been detected in ticks feeding on...
Article
Infection with hepatitis E virus (HEV) is common in both developing and industrialized nations. Genotypes 3 and 4 are increasingly being reported, particularly in high‐income countries where the precise extent of HEV transmission via food is currently unclear. Recently HEV has been found to be excreted in milk however data on the potential milk‐bor...
Article
This systematic review aims to present an overview of the current aerosol sampling methods (and equipment) being used to investigate the presence of SARS‐CoV‐2 in the air, along with the main parameters reported in the studies that are essential to analyze the advantages and disadvantages of each method and perspectives for future research regardin...
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The close contact between humans and domestic cats raises concerns about the potential risks of SARS-CoV-2 transmission. Thus, this study aims to investigate anti-SARS-CoV-2 IgG seroprevalence in client-owned cats from Portugal and evaluate the infection risk of cats that maintain contact with human COVID-19 cases. A total of 176 cats, belonging to...
Article
Rickettsiosis is considered an emerging/re-emerging vector-borne disease that causes significant public health threats. Ticks are reservoirs and vectors of Rickettsia having a significant role in the transmission of rickettsiae. In Portugal, little is known about tick-borne Rickettsia species in sheep. Therefore, this study aimed to investigate ric...
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Full-text available
Wastewater-based epidemiology has shown to be a promising and innovative approach to measure a wide variety of illicit drugs that are consumed in the communities. In the same way as for illicit drugs, wastewater-based epidemiology is a promising approach to understand the prevalence of viruses in a community-level. The ongoing coronavirus disease...
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Dirofilaria immitis is a zoonotic parasitic nematode that infects domestic and wild canids, among its vertebrate hosts. The genetic analysis of D. immitis nowadays transcends the need for genetic taxonomy of nematodes, such as the study of resistance to macrocyclic lactone. We expanded the use of long-read nanopore-based sequencing technology on ne...
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Toxoplasmosis is a worldwide zoonotic disease with different and complex routes for transmission. Workers occupationally exposed to animals or raw meat and viscera (WOE) may be at more risk than the general population, however conflicting data exist on the risk of developing toxoplasmosis due to this close contact. To add knowledge to this topic, t...
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Airborne transmission is mainly associated with poorly ventilated and crowded indoor environments where people stay for long periods of time. As such, public transport is often perceived as having a high risk for the transmission of SARS-CoV-2. Considering that data on the detection of SARS-CoV-2 in public transport systems are scarce, we performed...
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In addition to Helicobacter pylori, many non-Helicobacter pylori Helicobacters (NHPH) are able to cause gastric disease in humans. Cats are a natural reservoir for many of these species. Accordingly, living in close and intimate contact with animals has been identified as a risk factor, and an important zoonotic significance has therefore been attr...
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Background Dirofilaria immitis is a parasitic nematode endemic in the Mediterranean countries, which causes cardiopulmonary dirofilariosis in wild and domestic animals. Despite being recognized hosts of D. immitis, wild carnivores such as wolves and foxes are frequently disregarded when considering a potential role in the transmission of these zoon...
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E-cadherin (E-cad) is a cell-adhesion molecule known for its tumor-invasion suppressor function. E-cad expression was examined immunohistochemically in a series of canine tissue samples, including normal gastric mucosa (NGM; n = 3), gastric carcinomas (GC; n = 33), adjacent non-neoplastic mucosa (NNM; n = 32), neoplastic emboli (n = 16) and metasta...
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Canine Leishmaniosis (CanL) is a chronic and potentially fatal disease. In economically disadvantaged regions, costs associated with long-term patient monitoring may determine that some owners decline veterinary follow-up of their dogs. This online, questionnaire-based survey aimed to assess how Portuguese veterinary practitioners perform long-term...
Article
Human Pegivirus‐1 (HPgV‐1) is a member of the Flaviviridae family and the Pegivirus genus. Despite having been discovered 25 years ago, there is still much to know regarding HPgV‐1 clinical impact, as this virus is currently not associated with any pathology. Yet, HPgV‐1 prevalence and molecular characterization is still unknown in many countries,...
Article
The zoonotic parasitic nematode Thelazia callipaeda, also known as the oriental eye worm, is endemic in several European countries, including Portugal. Infections may result in ocular disease in domestic and wild animals as well as humans, with more or less severe manifestations. We report the first human case of ocular thelaziosis by T. callipaeda...
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Background: Cutaneous neoplastic diseases are the most and second-most frequently reported tumors in male and female dogs, respectively. The aims of this study were to report the occurrence of canine cutaneous tumors in a pathology laboratory located in Northern Portugal between 2014 and 2020, and to characterize and categorize the anatomical loca...
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Porcine circovirus type 2 (PCV-2) is associated with several syndromes affecting swine, also known as porcine-circovirus-associated diseases, of which post-weaning multi-systemic wasting syndrome stands out due to its high economic impact on swine production. Recent data suggest the increasing circulation of the PCV-2d genotype in several countries...
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As the third wave of the COVID-19 pandemic hit Portugal, it forced the country to reintroduce lockdown measures due to hospitals reaching their full capacities. Under these circumstances, environmental contamination by SARS-CoV-2 in different areas of one of Portugal’s major Hospitals was assessed between 21 January and 11 February 2021. Air sample...
Article
Hepatitis E virus (HEV) genotype 3 is the most prevalent HEV genotype in Europe causing mostly asymptomatic infections in humans, but can also sporadically cause severe acute hepatitis, chronic liver disease, chronic hepatitis in immunocompromised patients, and extra-hepatic manifestations. Although much is today known about the swine reservoir, no...
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Fasciola hepatica is a parasite that is widespread in Europe, having been reported in ruminants of several European countries and causing an important economic impact. This study ascertained the occurrence and distribution of fasciolosis in Portuguese ovine livestock by assessing F. hepatica IgG antibodies in a cohort of confined sheep from a high-...
Chapter
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Veterinary physiology is a basic curricular unit for every course within the veterinary field. It is mandatory to understand how the animal body works, and what to expect of a healthy body, in order to recognize any misfunction, and to be able to treat it. Classic physiology teaching involves wet labs, much equipment, many reagents, some animals, a...
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Coronavirus disease 19, or COVID-19, is an infection associated with an unprecedented worldwide pandemic caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which has led to more than 215 million infected people and more than 4.5 million deaths worldwide. SARS-CoV-2 cell infection is initiated by a densely glycosylated spike...
Article
Background: Skin tumors are the most frequently diagnosed lesions, of which 7%-21% are mast cell tumors (MCTs). There is a great effort to identify factors that can influence the prospective course of MCTs. Although, the histological grade is considering an important predictor helping to determine the malignancy and metastatic potential of MCTs....
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TFF1 expression is markedly reduced in human GCs, suggesting that TFF1 is a tumor suppressor for human gastric cancer. The present study evaluated the expression and distribution pattern of TFF1 in paraffin-embedded canine gastric tissue samples, including normal mucosa (n = 3), polyps (n = 8), carcinomas (n = 31) and their adjacent non-neoplastic...
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Animal crimes are a widespread phenomenon with serious implications for animal welfare, individual well-being and for society in general. These crimes are universal and represent a major problem in human/animal interaction. In Portugal, current law 69/2014 criminalizes the mistreatment and abandonment of companion animals. This study characterizes...
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More than 61% of all human pathogens are zoonotic, representing 75% of all emerging pathogens during the past decade. Albeit significant technological leaps in diagnostics development and disease surveillance, zoonotic emerging infectious diseases are evermore a matter of concern, particularly in modern days where global warming keeps providing ide...
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Brazil is the fifth largest country in the world with diverse socioeconomic and sanitary conditions, also being the fourth largest pig producer in the world. The aim of the present systematic review was to collect and summarize all HEV published data from Brazil (from 1995 to October 2020) performed in humans, animals, and the environment, in a One...
Article
Background: The SARS-CoV-2 pandemic spread across Europe from February 2020. While robust SARS-CoV-2 serological assays were quickly developed, only limited information on applied serological testing is available. We describe the extent and nature of SARS-CoV-2 serological testing used in Europe and assess the links between epidemiology, mitigatio...
Data
Supplementary Table- Summary of the results and findings of each publication included in the presented systematic review. Including the date of sampling, location and site of sampling, total number of samples collected, method used for sampling and detection of SARS-CoV-2, molecular targets, viability, number and percentage of positive samples, and...
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Little is known about contaminated surfaces as a route of transmission for SARS-CoV- 2 and a systematic review is missing and urgently needed to provide guidelines for future research studies. As such, the aim of the present study was to review the current scientific knowledge and to summarize the existing studies in which SARS-CoV-2 has been detec...
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Leptospira kirschneri is an agent causing leptospirosis in animals and humans. We report the draft genome sequence of Leptospira kirschneri serovar Mozdok type 2 strain Horse 112, comprising 485 contigs and having a genome size of 4,301,784 bp. This genome will facilitate studying important mechanisms for clinical outcomes.
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Free-roaming dogs and cats represent potential reservoirs for zoonotic vector-borne pathogens shedding to the human population. Given the health impact of these pathogens, we searched free-roaming dogs and cats included in an animal population control program from Luanda, Angola, for Bartonella and hemotropic mycoplasma infection. We report the det...
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Hedgehogs (e.g., Erinaceus europaeus, E. roumanicus) are wild mammals that frequently are observed near residential areas. The aim of this study was to investigate ticks and fleas collected from European hedgehogs in Portugal and to evaluate the prevalence of Rickettsia in those ectoparasites. Ticks and fleas were identified by morphological and mo...
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(1) Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological anti-T. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening...
Article
Ticks are one of the most common vectors of a broad variety of pathogenic agents that significantly affects cattle production causing reduced productivity and important economic losses, while simultaneously having an impact on human health due to the zoonotic risk. In much of the territory of Angola urban population has grown rapidly in recent deca...
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Tick-borne agents constitute a growing concern for human and animal health worldwide. Hyalomma aegyptium is a hard tick with a three-host life cycle, whose main hosts for adults are Palearctic tortoises of genus Testudo. Nevertheless, immature ticks can feed on a variety of hosts, representing an important eco-epidemiological issue regarding H. aeg...
Article
Background: Although an increasing body of data reports the detection of SARS-CoV-2 RNA in air, this does not correlate to the presence of infectious viruses, thus not evaluating the risk for airborne COVID-19. Hence there is a marked knowledge gap that requires urgent attention. Therefore, in this systematic review, viability/stability of airborne...
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Embryo transfer (ET) is a common procedure in rodent facilities. Optimizing this technique may help to reduce the number of animals, but little information is available regarding wild type strains and the conditions that affect embryo transfer. To explore this theme, 2-cell C57BL/6J embryos were transferred after overnight culture of freshly collec...
Article
Hepatitis E virus (HEV) deriving from manure application runoffs and faecal waste spill over of swine and human origin bypass wastewater treatment plants and contaminate coastal waters. Shellfish bioaccumulate enteric viruses such as HEV from fecally contaminated coastal waters and under current European Regulations, shellfish sanitary status surve...
Article
Dogs are highly exposed to pathogens transmitted by ectoparasites. The Mediterranean climate of Southern Europe, together with the presence of stray and/or neglected pets in close proximity with humans, contribute for tick expansion and stand for increased risk to infections in humans due to the zoonotic potential of many of these agents. The aim o...
Article
Environmental factors play a key role in the zoonotic transmission of emerging pathogenic viruses as mankind is constantly disturbing wildlife's ecosystems usually by cutting down forests to build human settlements or by catching wild animals for food, which deprives the viruses of their natural hosts and gives them opportunity to infect humans. In...
Article
Q fever is a zoonosis caused by Coxiella burnetii, and transmission to humans is often associated with contact with ovine and caprine livestock. Those exposed to sheep are particularly at high risk of infection. Recent studies show that Q fever is increasing in sheep farms in Portugal raising alerts on spillover to humans. We detected anti-C. burne...
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Bovine Ocular Squamous Cell Carcinoma is considered the most common bovine tumour, causing significant economic losses, mainly by abattoir condemnations. To obtain a better insight into the genesis and neoplastic transformation, 19 samples collected at slaughter from Holstein Friesian cattle and diagnosed as Ocular Squamous Cell Carcinoma were stud...
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Crenosoma striatum is a host-specifi c metastrongiloid nematode causing respiratory tract disease in hedgehogs ( Erinaceus europaeus ). Since few studies have reported C. striatum in hedgehogs and little genetic data is available concerning this lungworm, this study aimed to determine the occurrence of C. striatum in a population sample of hedgehog...
Article
Introduction Norovirus gastroenteritis is one of the most frequent causes of personnel unavailability in military units, being associated with significant morbidity and degradation of their operational effectiveness. The disease is usually mild but can be severe and life-threatening in young and healthy soldiers, who are prone to dehydration due to...
Data
Porcine astrovirus 2/BEL/15V010 isolate 15V010 ORF1, ORF1ab, and ORF2 genes, complete cds.
Data
LOCUS KT934307 1667 bp ds-RNA linear VRL 18-JUL-2016 DEFINITION Picobirnavirus wolf/PRT/416/2015 strain PBV/wolf/PRT/416/2015 RNA-dependent RNA polymerase (RdRp) gene, complete cds. ACCESSION KT934307 VERSION KT934307.1 KEYWORDS . SOURCE Picobirnavirus wolf/PRT/416/2015 ORGANISM Picobirnavirus wolf/PRT/416/2015 Viruses; dsRNA viruses; Picobirnaviri...
Data
LOCUS KY214440 1098 bp RNA linear PHG 29-JUL-2017 DEFINITION Caninovirus sp. isolate South Douro RNA2 capsid gene, complete cds. ACCESSION KY214440 VERSION KY214440.1 KEYWORDS . SOURCE Caninovirus sp. ORGANISM Caninovirus sp. Viruses; unclassified bacterial viruses. REFERENCE 1 (bases 1 to 1098) AUTHORS Conceicao-Neto,N., Godinho,R., Alvares,F., Yi...
Data
LOCUS KX458103 998 bp RNA linear VRL 13-OCT-2016 DEFINITION Norovirus GI.9 strain PoHuTejo2015 nonstructural polyprotein and capsid protein genes, partial cds. ACCESSION KX458103 VERSION KX458103.1 KEYWORDS . SOURCE Norovirus GI.9 ORGANISM Norovirus GI.9 Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Caliciviridae; Norovirus....
Data
LOCUS KY214432 1640 bp RNA linear VRL 29-JUL-2017 DEFINITION Porcine picobirnavirus isolate BEL/15V010/5682 RdRp gene, complete cds. ACCESSION KY214432 VERSION KY214432.1 KEYWORDS . SOURCE Porcine picobirnavirus ORGANISM Porcine picobirnavirus Viruses; dsRNA viruses; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus. REFERENCE 1 (bases...
Data
LOCUS KF792838 1236 bp RNA linear VRL 14-MAY-2014 DEFINITION Feline picobirnavirus isolate 2012 RNA-dependent RNA polymerase gene, partial cds. ACCESSION KF792838 VERSION KF792838.1 KEYWORDS . SOURCE Feline picobirnavirus ORGANISM Feline picobirnavirus Viruses; dsRNA viruses; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus. REFERENCE...
Data
LOCUS KX815297 340 bp RNA linear VRL 05-FEB-2017 DEFINITION Hepatitis E virus isolate PoHuG12016 RNA-dependent RNA polymerase (ORF1) gene, partial cds. ACCESSION KX815297 VERSION KX815297.1 KEYWORDS . SOURCE Hepatitis E virus ORGANISM Hepatitis E virus Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Hepeviridae; Orthohepevirus;...
Data
LOCUS LN887197 336 bp RNA linear VRL 08-OCT-2015 DEFINITION Hepatitis E virus partial ORF1 gene for RNA-dependent RNA polymerase (RdRp) gene, isolate S11. ACCESSION LN887197 VERSION LN887197.1 KEYWORDS . SOURCE Hepatitis E virus ORGANISM Hepatitis E virus Viruses; Riboviria; Hepeviridae; Orthohepevirus; Orthohepevirus A. REFERENCE 1 AUTHORS Mesquit...
Data
LOCUS LN887198 338 bp RNA linear VRL 08-OCT-2015 DEFINITION Hepatitis E virus partial ORF1 gene for RNA-dependent RNA polymerase (RdRp) gene, isolate S34. ACCESSION LN887198 VERSION LN887198.1 KEYWORDS . SOURCE Hepatitis E virus ORGANISM Hepatitis E virus Viruses; Riboviria; Hepeviridae; Orthohepevirus; Orthohepevirus A. REFERENCE 1 AUTHORS Mesquit...
Article
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Hepatitis E virus genotype 3 infections are normally asymptomatic in immunocompetent individuals. Symptomatic cases of acute icteric hepatitis E are seldom observed among women, younger men and children but are particularly seen in middle-aged/elderly men. We report a case of severe acute hepatitis E caused by genotype 3 in an immunocompetent 40-ye...
Article
Hepatitis E virus (HEV) is an enteric RNA virus from the family Hepeviridae with five genotypes (genotypes 1‐4 and 7) known to infect humans. HEV infection is known to have a zoonotic swine origin in industrialized countries. The role of pigs and wild boars as major reservoirs for human infection is today well‐established, however the list of new a...
Article
Sea urchin gonads are today considered a gourmet food but despite eaten raw, not much attention has been given to microbiological quality. This study evaluated the microbial (bacterial and viral) contamination and adequacy to current European regulations of Paracentrotus lividus sea urchins produced in Portugal. Four batches of 10 sea urchins were...
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Full-text available
Leptospirosis is a neglected zoonotic disease of worldwide distribution with a significant veterinary and public health impact. It is caused by pathogenic bacteria of the genus Leptospira. The availability of effective tools to accurately identify and type leptospires is of utmost importance for the diagnosis of the disease and for assessing its ep...
Article
The present study aims to contribute to the evaluation of the serological impact of vaccination against mumps in Portugal, measuring anti-mumps IgG (MuIgG) levels in cord sera and the corresponding proportions of seropositive newborns, and their association with potential predictive variables. The data from this study came from 198 umbilical cord s...
Article
Dissemination of vector arthropods harboring zoonotic pathogens through the uncontrolled transboundary trade of exotic and pet animals poses an important threat to Public Health. In the present report we describe the introduction of pathogenic Rickettsia africae and R. aeschlimanni in ticks removed from imported tortoises in Qatar. A total of 21 ti...
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The hepatitis E virus (HEV) affects almost 20 million individuals annually, causing approximately 3.3 million acute liver injuries, 56,600 deaths, and huge healthcare-associated economic losses. Shellfish produced close to urban and livestock areas can bioaccumulate this virus and transmit it to the human population. The aim of this study was to ev...
Article
Introduction Norovirus outbreaks frequently occur in communities and institutional settings acquiring a particular significance in armed forces where prompt reporting is critical. Here we describe the epidemiological, clinical and laboratorial investigation of a multicentre gastroenteritis outbreak that was detected simultaneously in three Portugue...
Article
Introduction: In 2011, Schmallenberg virus (SBV) was first detected in dairy cattle herds in The Netherlands and Germany having since then spread across Europe. Today studies are starting to show a decrease in new SBV infections, a circumstance that raises alerts for possible re-emergence if ideal conditions for vector development occur. To assess...
Article
A total of 100 domestic cats from Luanda (Angola) were tested for the presence of antibodies against Bartonella henselae and spotted fever group of Rickettsia (SFGR) using indirect immunofluorescence assay (IFA). Molecular screening targeting the riboflavin synthase (ribC) gene for Bartonella and outer membrane protein B (ompB) gene for Rickettsia,...