João C. F. NunesUniversity of Aveiro | UA · CICECO - Centre for Research in Ceramics and Composite Materials
João C. F. Nunes
Master of Science in Molecular Biomedicine
About
16
Publications
3,757
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
149
Citations
Introduction
João C. F. Nunes is a PhD fellow at the research group "Process and Product Applied Thermodynamics" (PATh), CICECO-Aveiro Institute of Materials, University of Aveiro (UA), Portugal. His research activities are mostly focused on the development of a cost-effective and scalable platform for the downstream processing of L-Asparaginase. Nunes published 10 journal-articles, 4 book-chapters, 1 encyclopedia entry, 1 international meeting abstract, 25 posters and 23 oral presentations (149 citations).
Skills and Expertise
Additional affiliations
January 2020 - August 2020
Position
- Research fellow
Description
- My research activities were mainly on the development of sustainable nanomaterials for the purification of antileukemic drugs.
April 2019 - January 2020
University of Aveiro
Position
- Research fellow
Description
- My research activities were mainly on purification of high value proteins using nanomaterials, which comprised characterization of their properties and assessment of their potential in the establishment of extractive platforms for value-added compounds (biopharmaceuticals).
Education
September 2016 - July 2018
University of Aveiro
Field of study
- Molecular Biomedicine
September 2011 - June 2015
University of Aveiro
Field of study
- Nursing
Publications
Publications (16)
l-asparaginase (ASNase, EC 3.5.1.1) is an aminohydrolase enzyme with important uses in the therapeutic/pharmaceutical and food industries. Its main applications are as an anticancer drug, mostly for acute lymphoblastic leukaemia (ALL) treatment, and in acrylamide reduction when starch-rich foods are cooked at temperatures above 100 °C. Its use as a...
L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a b...
L-asparaginase (ASNase) is an amidohydrolase that can be used as a biopharmaceutical, as an agent for acrylamide reduction, and as an active molecule for L-asparagine detection. However, its free form displays some limitations, such as the enzyme’s single use and low stability. Hence, immobilization is one of the most effective tools for enzyme rec...
Proteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming the current drawbacks of purification processes of proteins, the introduction of ionic liquids (ILs) has been a hot topic of research. ILs have been applied in the creation of aqueous biphasic systems (IL-based ABS), solid-phase extra...
Carbon nanotubes (CNTs) were discovered in 1991, and since then, have been one of the most intensely studied nanomaterials due to their improved functionalities and diversity of applications. Specifically, CNTs are entirely composed of carbon atoms connected through sp2 bonds structured in several condensed benzene rings rolled up into a cylinder f...
Microbial biosurfactants have been emerging as promising compounds for drug-delivery systems (DDS) advances. They can be formed using diverse classes of microorganisms, being versatile biocompounds with specific surface characteristics. When compared to chemical surfactants, good biodegradability, small toxicity, and potential biological activity a...
l-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the l-asparagine hydrolysis into l-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some disadvantages are associated with its free form, such as the ASNase short half-life, which may be overcome by enzyme immobilization. In this work, the...
Background:
Antibiotic resistance is a worldwide public health problem, leading to longer hospital stays, raising medical costs and mortality levels. As physicians' attitudes are key factors to antibiotic prescribing, this study sought to explore their differences between primary care and hospital settings.
Methods:
A survey was conducted betwee...
l-Asparaginase (ASNase, EC 3.5.1.1) is a tetrameric aminohydrolase enzyme that catalyses the hydrolysis of the amino acid L-Asparagine into ammonia and L-aspartic acid. ASNase is present in different organisms such as bacteria, fungi, plant tissues and algae. ASNase is used in the pharmaceutical field as an anticancer drug for the treatment of acut...
Biosurfactants produced by different types of microorganisms are amphiphilic biomolecules with active surface properties. Recently, biosurfactants have emerged as promising agents for cancer therapy since a high diversity of these molecules has shown the ability to induce cytotoxicity against many cancer cell lines, thus regulating cancer progressi...
The enzyme L-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Car...
Antibodies present in mammal’s serum are of high relevance for therapeutic purposes, particularly in passive immunization and in the treatment of some chronic diseases. However, their recovery with high purity...
