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January 2004 - present
January 1993 - May 1995
Publications
Publications (19)
This tutorial describes a colorometric enzyme assay to determine simple enzyme kinetics. It is designed as an introduction for beginning laboratory students in a biology or biochemistry course, and requires no previous understanding of chemistry. It may be used as an assignment to prepare students for a laboratory exercise in enzyme kinetics Throug...
Benzyl selenocyanates can be made from the corresponding benzylic bromides or chlorides in 30-60 minutes using acetonitrile as a solvent. The products may be obtained pure in satisfactory yields without recourse to chromatography.
Computers have been installed in the General Biology laboratory at Purchase College and incorporated into the laboratory curriculum for all biology majors at the introductory level. The goal is to ensure that all students become familiar with general computer applications in the biological sciences and are comfortable enough to use them regularly....
Avian leukemias and lymphomas are caused primarily by retroviruses and herpesviruses. The protooncogenes activated by avian retroviral insertions in B & T-cell lymphomas will be summarized, with discussion on a new common insertion site, bravo, associated with RAV-O LTR insertion. Two novel interactions between avian retroviruses and Marek's diseas...
To explore cellular effects of potent organoselenium chemopreventive agents we have used a rat mammary tumor cell line. We demonstrate that 1,4-phenylenebis(methylene) selenocyanate (p-XSC) at a dose of 5 microM is a more potent inhibitor of DNA, RNA and protein synthesis as well as of mitochondrial transmembrane potential than its chemopreventive...
To explore cellular effects of potent organoselenium chemo-preventive agents we have used a rat mammary tumor cell line. We demonstrate that 1,4-phenylenebis(methylene) selenocyanate (p-XSC) at a dose of 5 μM is a more potent inhibitor of DNA, RNA and protein synthesis as well as of mitochondrial transmembrane potential than its chemopreventive cou...
At present there is considerable controversy surrounding the role of dietary fat in breast cancer (1,2). Epidemiological, anthropological, and animal model studies support the concept that high fat intake increases breast cancer risk (3–6). However, several cohort studies, in consistent with such an hypothesis have recently been reported (1,7). In...
To identify mechanisms by which the organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) mediates its chemopreventive activities, we have examined its effects on cell lines derived from breast cancer of humans and rats. When log-phase cells were treated with a dose of 1 microM p-XSC, we observed a significant decrease in thymidi...
Release of DNA fragments into the culture supernatant of human promyelocytic cell line HL-60 was investigated. The released DNA has previously been shown to have a strong immunosuppressive effect on the proliferation of mitogen-stimulated human peripheral lymphocytes and some cancer cell lines. In this study, HL-60 cells were cultured for four days...
In previous studies, we have demonstrated that, while dietary fats were not found to affect the frequency of ras mutation in rat mammary tumors induced by N-nitrosomethylurea (NMU), they did affect final incidence of tumors, supporting the idea that the diet mediates promotion-related changes. As altered p53 expression has been described in 45–55%...
A rat mammary tumor cell line maintained in low serum (1%) medium exhibited a slower growth rate, altered cell cycle distribution, decreased DNA synthesis, and increased immunoprecipitable p53, when compared with culture in 10% serum. Addition of linoleic acid (LA; 18:2, n-6) to low serum medium partially restored normal cell cycle distribution, in...
To determine the effects of increased sympathetic nervous system activity in humoral hypertension on the regulation of surface alpha 1 adrenoceptors and signal transduction, deoxycorticosterone acetate (DOCA) salt hypertension was induced in rats and the animals killed three weeks following the initiation of hypertension.
Experiments were performed...
Marek disease virus (MDV) is a herpesvirus of chickens that induces T lymphomas within 3 weeks of infection. The short latency and polyclonal nature of MDV-induced tumors have suggested that the virus may encode one or more direct-acting oncogenes. To date, however, no MDV-specific tumor antigens or candidate transforming genes have been demonstrat...
A suppressor activity was isolated and partially purified from culture supernatant of human myeloid leukaemic cell line HL-60 by ion-exchange chromatography, HPLC and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This activity was capable of suppressing proliferation of mitogen-stimulated normal human lymphocytes in a dose-d...
The growth rate of DU145 prostate cancer cells in vitro is slowed considerably by changing the growth medium every 24 h, suggesting dependence upon endogenously-secreted growth factors. Because previous studies have identified epidermal growth factor (EGF) in the conditioned medium from DU145 cells, [35S]labeled EGF was selectively immunoprecipitat...
Androgen-independent prostate cancer cells may rely on an autocrine loop for growth stimulation, and have been shown to express both the epidermal growth factor receptor (EGFR) and its stimulatory ligands. We have shown here that DU 145 prostate cancer cells have a decreased amount of EGFR in confluent cultures when compared to levels seen in subco...
To determine the effects of myocardial infarction-induced left ventricular failure on the regulation of surface alpha-1 adrenoreceptors and signal transduction, large infarcts were produced in rats and the animals killed seven days later. After the documentation of impaired left ventricular pump performance, radioligand binding studies of the alpha...
Thesis (Ph. D.)--Michigan State University. Dept. of Biochemistry, 1987. Includes bibliographical references (leaves 145-153). Microfilm. s
