Joanna Nynca

Joanna Nynca
Polish Academy of Sciences | PAN · Institute of Animal Reproduction and Food Research

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103
Publications
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Introduction
Skills and Expertise

Publications

Publications (103)
Article
Full-text available
The natural resources of a country, including ichthyofauna, constitute a vital aspect of its national heritage. Fish populations are threatened with loss of biodiversity as a result of human activity (anthropopressure), resulting in water pollution, habitat destruction and overfishing. Additionally, the escalating threat is exacerbated by climate c...
Article
Rainbow trout are characterized by high amenability to chromosome manipulations during early development to produce androgens, gynogens, or sterile triploids, which are relevant in aquaculture. An understanding of the mechanisms affecting oogenesis and those resulting in diminished gonadal development in triploids is an important area of scientific...
Chapter
Two-dimensional difference gel electrophoresis (2D-DIGE) appears to be especially useful in quantitative approaches, allowing the co-separation of proteins of control samples and proteins of treated/disease samples on the same gel, eliminating gel-to-gel variability. The principle of 2D-DIGE is to label proteins prior to isoelectric focusing and us...
Article
In order to obtain sex-reversed females of Salmonid fish (SRF; neomales, masculinized females) the hormonal treatment of fertilized eggs or larvae is used. As a consequence, masculinized fish possess less-developed testes without functional spermatic ducts. Therefore, the semen of sex-reversed females is generally characterized by low or highly var...
Article
Triploidisation represents several advantages (e.g. sterility) and therefore is routinely applied in aquaculture of several commercially important fish species, including rainbow trout. The comparative transcriptomic analysis of ovaries of triploid (3N) and diploid (2N) female rainbow trout revealed a total of 9 075 differentially expressed genes (...
Article
Full-text available
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-sm...
Article
The aim of this study was to evaluate the cryopreservation of masu salmon (Oncorhynchus masou) semen, using 0.18 M glucose in 9% methanol as an extender, and to characterize semen parameters (sperm motility; sperm concentration; and seminal plasma parameters such as pH, electric conductivity, DNA concentration, and protein concentration), with resp...
Article
The composition of the ovarian fluid (OF) is predestined for egg storage and for prolonging the fertilization period during natural spawning as well as during artificial fertilization. A thorough characterization of the specific components within the OF and eggs may provide deeper knowledge about their functions, a better understanding of egg-OF si...
Article
The aim of this study was to investigate the respective contribution of maternally-inherited mRNAs and proteins to egg molecular cargo and to its developmental competence in fish using pikeperch as a model. Our study provides novel insights into the understanding of type-specific roles of maternally-inherited molecules in fish. Here we show, for th...
Preprint
Full-text available
Background In Metazoans, embryonic development relies on maternally-inherited mRNAs and proteins that are critical for early developmental success and known to play major roles at later stages, beyond zygotic genome activation. However, very poor concordance between transcript and protein levels in oocytes and embryos of vertebrates suggest that ma...
Article
Full-text available
Sex reversal has been used as a breeding strategy by salmonid fish to produce genetically and phenotypically single sex populations. Production of all-female fish has great importance for the creation of monosex female triploids of salmonid fish, which are valued for their sterility, lack of female maturation, and larger commercial size. Among salm...
Article
Refreezing freeze-thawed sperm has been found to improve the effectiveness of cryopreserved semen in mammals. However, such options have been never tested in fish. The objectives of this study were (i) to assess the effects of two freeze–thaw cycles on sperm motility parameters of different fish species (grayling, brown trout, Eurasian perch, Siber...
Article
Pikeperch, Sander lucioperca, is a species of high interest to the aquaculture. The expansion of its production can only be achieved by furthering domestication level. However, the mechanisms driving the domestication process in finfishes are poorly understood. Transcriptome profiling of eggs was found to be a useful tool allowing understanding of...
Chapter
Sperm quality is primarily defined as its capability of fertilising an egg and consequently allowing for the development of a normal embryo. Sperm quality can be highly variable; therefore, gamete quality assessment is a prerequisite for fertilisation trials. Fish semen analysis includes several methods, such as measurements of sperm motility param...
Article
Full-text available
Domestication is a condition in which the breeding, care and feeding of animals are, at least in part, controlled by humans. Information regarding the changes in the protein composition of eggs in response to domestication is very limited. Such data are prerequisite for improvements in the reproduction of domesticated fish. The aim of this study wa...
Article
The aim of the current study was to determine the sperm quality of sex-reversed female (SRF) and normal male rainbow trout measured as ROS⁺ (reactive oxygen species) production and TAC (total antioxidant capacity) in semen cryopreserved at different glucose concentration [optimal (0.15 M), low (0.11 M) and high (0.19 and 0.21 M for SRF and normal m...
Article
Salmonid fish spermatozoa acquire the potential for motility during the transition from the testis to the spermatic duct. Due to the lack of a spermatic duct in sex-reversed females (srf) of rainbow trout, sperm resemble testicular semen and must be collected directly from the testes; consequently, they are immature with no or low potential for mot...
Article
The objective of this work was to develop a complex standardisation of cryopreservation procedure for rainbow trout semen. The specific objectives were to determine the effects of the final glucose concentration (0.11–0.23 M), final methanol concentration in the extender (0–20%), equilibration time (5–180 min), and post-thaw storage time of semen (...
Article
Full-text available
A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with non-cancerous lung diseases, and the experimental gr...
Article
The general objective of this study was to establish a standardized procedure for the cryopreservation of Arctic charr milt and its use in the production of ‘sparctic’ hybrids. The specific objectives of this study were to determine: (i) the optimal final sperm concentration as well as (ii) the final glucose concentration, (iii) the effect of post-...
Article
Full-text available
Varroa destructor is an external parasite of Apis mellifera and feeds on the haemolymph of pupae and adult bees, vectoring numerous viral pathogens, in particular the deformed wing virus (DWV). However, the changes in haemolymph protein composition after Varroa infestation are unknown. The objective of this study was to investigate alterations in t...
Article
Rainbow trout sperm are 'maladapted' to freshwater spawning, resulting in shorter duration of sperm motility in fresh water compared to buffered saline solution. We hypothesized that different sperm motility-activating media have various effects on sperm motility characteristics and oxidative stress, as well as on the protein profiles of rainbow tr...
Article
Proteomics and metabolomics are emerging and powerful tools to unravel the complex molecular mechanisms regulating reproduction in male fish. So far, numerous proteins and metabolites have been identified that provide us with valuable information to conduct a comprehensive analysis on seminal plasma and spermatozoa components and their functions. T...
Article
The main aim of the present review is to present the opportunities and challenges associated with the application of cryopreserved sperm, which may improve the breeding of salmonid fishes. Cryopreservation of sperm has been used as a strategy for the conservation of biodiversity of fishes populations, the preservation of sperm from the most valuabl...
Article
Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chro...
Article
The aim of our study was to compare quality of fresh and cryopreserved semen of sex-reversed female rainbow trout and sex-reversed female brook trout. The effect of different final sperm concentrations in straws with a constant extender concentration and final glucose concentration on the sperm motility parameters of cryopreserved semen was tested....
Article
Full-text available
Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and the epithelial-mesenchymal transition (EMT) process. In th...
Chapter
Two-dimensional difference gel electrophoresis (2D-DIGE) appears to be especially useful in quantitative approaches, allowing the co-separation of proteins of control samples from proteins of treatment/disease samples on the same gel, eliminating gel-to-gel variability. The principle of 2D-DIGE is to label proteins prior to isoelectric focusing and...
Article
Ensuring the precise conditions during cryopreservation relies on constant sperm concentration in straws with constant cryoprotectant concentrations, which are essential for standardizing cryopreservation protocols. The aim of our study was to test the effect of different sperm concentrations in straws with constant extender concentration on sperm...
Article
Full-text available
SDS-PAGE combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 2-dimensional electrophoresis (2DE) combined with matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry (MALDI TOF/TOF) were applied to characterize the turkey seminal plasma proteome. LC-MS/MS led to the identification of 175...
Article
Masculinized females, named sex-reversed females (SRF), have a male phenotype but retain the female genotype (XX) and all spermatozoa produced in their testes carry the X chromosome. Masculinization of females leads to incomplete testicular development and the production of lower-quality semen. The mechanism of masculinization is unknown at present...
Article
The characterization of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study LC-MS/MS and 2D DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified...
Article
Transferrin (TF) is recognized as a multifunctional protein and has been implicated in antioxidative, antimicrobial protection, growth, differentiation and cytoprotection effects. An efficient, original three-step isolation procedure for TF consisting in hydrophobic interaction chromatography, gel filtration and preparative electrophoresis was deve...
Article
Determination of exact sperm concentrations in straws is necessary for standardizing protocols of fish cryopreservation. The aim of this study was to test the effect of final sperm concentration in straws on the post-thaw quality of rainbow trout semen in conditions of constant cryoprotectant concentration (0.15 M glucose and 7.5% methanol). In add...
Chapter
Two-dimensional difference gel electrophoresis (2D-DIGE) appears to be especially useful in quantitative approaches, allowing the co-separation of proteins of control samples from proteins of treatment/disease samples on the same gel, eliminating gel-to-gel variability. The principle of 2D-DIGE is to label proteins prior to isoelectric focusing and...
Article
Masculinized females, named sexreversed females (SRF), have a male phenotype but retain the female genotype (XX) and all spermatozoa produced in their testes carry the X chromosome. Masculinization of females leads to incomplete testicular development and the production of lower-quality semen. The mechanism of masculinization is unknown at present....
Article
Potassium ions are known to have an inhibitory effect on the sperm motility of salmonids. For this reason, the addition of K(+) to the extender is frequently applied. However, the effect of the addition of K(+) to the extender has not yet been tested. The aim of this study was to test the influence of potassium ion supplementation of the extender o...
Article
The effect of replacement of glucose in glucose-methanol extender (GM) with either sucrose and trehalose on the sperm motility characteristics of cryopreserved semen and the post-thaw storage of salmon, brown trout, brook trout, sex-reversed female rainbow trout and whitefish was investigated. Semen samples were diluted 1:5 or 1:9 (for sex-reversed...
Article
The effect of replacement of glucose in glucose-methanol extender (GM) with either sucrose and trehalose on the sperm motility characteristics of cryopreserved semen and the post-thaw storage of salmon, brown trout, brook trout, sex-reversed female rainbow trout and whitefish was investigated. Semen samples were diluted 1:5 or 1:9 (for sex-reversed...
Article
The objectives of this study were to determine the specific characteristics of sperm motility and fertilization rates of whitefish (Coregoninae subfamily, Salmonidae, Salmoniformes) and northern pike (Esocidae, Esociformes) in response to cryopreservation using a glucose–methanol extender, which has recently been successfully used for salmonid spec...
Article
Proteomic approaches have recently been introduced to studies of male fish reproduction, providing new insights into the functioning of the reproductive system. Numerous previously unknown proteins have been identified, allowing for the comprehensive characterization of the mechanisms involved in the protection of spermatozoa in seminal plasma, esp...
Article
Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species and antioxidant defences. We measured total antioxidant capacity (TAC) in honeybee haemolymph and seminal plasma and analysed TAC of haemolymph in relation to age and exposure to pesticide. TAC of haemolymph increased with age of bees (1.18...
Article
The objective of this study was to test the effect of cryopreservation with the use of a methanol–glucose extender on sperm motility parameters and fertilization rates of European huchen (Hucho hucho) and grayling (Thymallus thymallus), belonging to the Salmonidae family of Salmoniformes. Distinct differences between those species in absolute ferti...
Article
Full-text available
The objectives of this study were to (1) evaluate if dietary lysine (Lys) has an effect on the free amino acid (FAA) pool of Yellow Perch Perca flavescens eggs, and (2) determine how dietary Lys influences the reproductive performance and eggs fertilization rate (embryo survival) of female Yellow Perch. Two-year-old Yellow Perch of initial size of...
Article
Turkey seminal plasma contains three serine proteinase inhibitors. Two of them, with low molecular weights (6 kDa), were identified as single-domain Kazal-type inhibitors responsible for regulating acrosin activity. Our experimental objective was to isolate and characterize the inhibitor with the high molecular weight from turkey seminal plasma. Th...
Article
Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins by SDS-PAGE pre-fractionation combined with nano-LC-MS/MS based identification. This study provides the first in-de...
Article
Full-text available
The objective of the present study was to determinewhether lysine (Lys) supplementation influences thematuration and sperm quality of Yellow Perch Perca flavescens. Juveniles raised on a formulated commercial diet and weighing approximately 75 g each were randomly distributed into six 400-L tanks. This experiment included two wheat-glutenbased diet...
Article
Full-text available
In the present study we used a shotgun proteomic approach to identify 54 proteins of rainbow trout ovarian fluid. The study has unravelled the identity of several proteins not previously reported in fish ovarian fluid. The proteome of trout ovarian fluid consists of diverse proteins participating in lipid binding and metabolism, carbohydrate and io...
Article
The goal of this study was to improve post-thaw quality of cryopreserved rainbow trout semen. Quality was assessed by measuring sperm motility and fertilization rates at the eyed, hatching, and swim-up larvae stages. We first tested how varying glucose concentrations in a methanol extender (0.1, 0.2, and 0.3 M, n = 9 different males per concentrati...
Article
In the present study the combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography electrospray ionization tandem mass spectrometry was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins (...
Article
The objective of this work was to test the effects of three dilutions of semen to glucose–methanol extender ratios 1:3, 1:1 and 3:1 on the sperm motility characteristics of diluted and cryopreserved whitefish spermatozoa. The final concentration of extender compounds was achieved using concentrated extenders. The application of 3:1 dilution ratio a...
Article
Total antioxidant capacity (TAC) was measured in the seminal plasmaof 13 fish species using a commercial assay kit. TAC values are quite variable among the 13 species studied, from 0.008 mM of Trolox for rainbow trout to 1.909 mM of Trolox for Eurasian perch. Two species of percid fish were distinguished by high values of TAC. Most of TAC was attri...
Article
The aim of this study was to detect cryopreservation-induced alterations in the protein composition of rainbow trout semen using two independent methods 1-DE SDS-PAGE prefractionation combined with LC-MS/MS and two-dimensional difference gel electrophoresis followed by MALDI-TOF/TOF identification. Here we show the first comprehensive dataset of ch...
Article
The aim of this study was to characterize the fresh and cryopreserved whitefish semen and to verify the usefulness of fresh semen parameters in order to qualify the cryopreserved semen. The relationship between DNA microsatellite geno-typing and quality parameters of fresh and cryopreserved semen was also examined. Whitefish semen was characterized...
Article
The quality of fish spermatozoa has been assessed by evalu-ating sperm motility and viability with the aim to improve the success of controlled fertilization in hatcheries. However, the relationship between various parameters is still unclear. In this study, we described simultaneous changes in sperm viability (using a NucleoCounter SP-100) and mot...
Article
This contribution presents an overview of the potential capabilities of evolving methodologies to characterize seminal protein profiles and identify interactions while simultaneously analyzing the complex but comprehensive protein matrix. The traditional approach to protein characterization in fish semen is based on two major steps: the purificatio...
Article
The use of soy-based products in pig diets had raised concerns regarding the reproductive toxicity of genistein, the predominant isoflavone in soybeans. Genistein was reported to exhibit weak estrogenic activity but its mechanism of action is not fully recognized. The aim of the study was to examine the in vitro effects of genistein on (1) progeste...
Article
Semen samples (collected using a catheter, n = 5) were experimentally contaminated with urine (10 – 30%) and incubated for 2 and 4 h. The percentage of sperm motility was measured at pH 6.5 and in D532 solution (pH 9.0). Contamination of rainbow trout milt with urine led to dose-dependent decrease in seminal plasma osmolality, from 301 ± 3 to 203 ±...
Article
The production of all-female populations has important economic benefits in commercial rainbow trout aquaculture. The procedure commonly implemented to produce all-female stocks centers on the sex reversal of rainbow trout females via the administration of androgens in the early developmental stages, followed by the egg fertilization of normal fema...
Article
This works studies the biochemical (protein concentration, osmolality, antitrypsin activity, lactate dehydrogenase activity) and physiological characteristics (sperm motility characteristics) of semen of sex-reversed female rainbow trout (n=42) obtained with the application of 11β-hydroksyandrostendione for sex reversal. All data were arbitrarily d...
Article
Full-text available
The lipocalin protein family is a large and diverse group of small extracellular proteins characterised by their ability to bind hydrophobic molecules. In the present study, we describe the isolation procedure for rainbow trout seminal plasma protein, characterised by a moderate migration rate during polyacrylamide gel electrophoresis, providing in...
Article
Calcium ions play an important role in the activation of fish sperm movement. The mechanism of their binding in semen is still unknown. The goal of this study was the development of a method for identifying calcium-binding proteins in fish seminal plasma. Two methods of calcium-binding proteins detection were tested with the use of Quin2 and Stains...
Article
In this study trypsin-inhibitory activity of the seminal plasma of percid fish: perch (Perca fluviatilis, n = 22), pikeperch (Sander lucioperca, n = 5), Volga pikeperch (Sander volgensis, n = 7) and hybrid between S. lucioperca ($) and S. volgensis (#) (n = 11) was identified and measured. Comparison of profiles of anti-trypsin activity (APA) in bl...