Joanna C Chiu

Joanna C Chiu
University of California, Davis | UCD · Department of Entomology

PhD

About

169
Publications
24,565
Reads
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5,429
Citations
Citations since 2016
99 Research Items
3075 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
Additional affiliations
July 2016 - present
University of California, Davis
Position
  • Professor (Associate)
September 2010 - July 2016
University of California, Davis
Position
  • Professor (Assistant)
June 2004 - May 2010
Rutgers, The State University of New Jersey
Position
  • PostDoc Position

Publications

Publications (169)
Preprint
Full-text available
Organisms adapt to seasonal changes in photoperiod and temperature to survive; however, the mechanisms by which these signals are integrated in the brain are poorly understood. We previously reported that EYES ABSENT (EYA) in Drosophila shows higher levels in cold temperature or short photoperiod, and genetic ablation of eya in the fly brain inhibi...
Preprint
Full-text available
Circadian clock and chromatin remodeling complexes are tightly intertwined systems that regulate rhythmic gene expression. The circadian clock promotes rhythmic expression, timely recruitment, and/or activation of chromatin remodelers, while chromatin remodelers regulate accessibility of clock transcription factors to the DNA to influence expressio...
Preprint
Full-text available
Animals are motivated to eat based on their internal need. When animals are hungry, the sensory detection of food elicits bursts of physiological changes in their bodies. This is the cephalic phase response (CPR), which prepares animals to properly digest food before nutrients enter their bodies. Despite animals uniformly exhibiting a strong CPR, t...
Chapter
Full-text available
Drosophila melanogaster has been an instrumental animal model for defining key properties of circadian rhythms and for investigating the cellular and molecular mechanisms regulating circadian timekeeping. Over the past few decades, locomotor activity rhythm has served as a standard phenotypic readout that reflects the functionality of the endogenou...
Article
Full-text available
O-linked- N -acetylglucosaminylation (O-GlcNAcylation) is a nutrient-sensitive protein modification that alters the structure and function of a wide range of proteins involved in diverse cellular processes. Similar to phosphorylation, another protein modification that targets serine and threonine residues, O-GlcNAcylation occupancy on cellular prot...
Article
Full-text available
Animals adapt their seasonal physiology by measuring photoperiodic changes over the calendar year. A new study in PLOS Biology uncovers changes in glutamate dynamics in the bean bug that are dependent on photoperiod and a clock gene. Organisms adapt to seasons to survive. This is a widespread phenomenon that relies predominantly on the ability to m...
Article
The spotted-wing drosophila, Drosophila suzukii (Matsumura), is a global economic pest of berry crops and stone fruit. Since management of this pest primarily relies on calendar insecticide applications, and field-derived resistance to spinosad has already been documented in California caneberry production, there is significant concern for developm...
Preprint
PERIOD (PER) and Casein Kinase 1δ regulate circadian rhythms through a phosphoswitch that controls PER stability and repressive activity in the molecular clock. CK1δ phosphorylation of the Familial Advanced Sleep Phase (FASP) serine cluster embedded within the Casein Kinase 1 binding domain (CK1BD) of mammalian PER1/2 inhibits its activity on phosp...
Article
Full-text available
Temperature compensation is a critical feature of circadian rhythms, but how it is achieved remains elusive. Here, we uncovered the important role played by the Drosophila PERIOD (PER) phosphodegron in temperature compensation. Using CRISPR-Cas9, we introduced a series of mutations that altered three Serines (S44, 45 and 47) belonging to the PER ph...
Article
Spinosyn insecticides are widely used in conventional berry production, and spinosad is regarded as the most effective insecticide for managing Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), spotted-wing drosophila, in organic berry crops. Following the 2017 identification of spinosad resistance in caneberry fields in the Watsonville area...
Preprint
Full-text available
Temperature compensation is a critical feature of circadian rhythms, but how it is achieved remains elusive. Here, we uncovered the important role played by the Drosophila PERIOD (PER) phosphodegron in temperature compensation. Using CRISPR-Cas9, we introduced a series of mutations that altered three Serines (S44, 45 and 47) belonging to the PER ph...
Preprint
The circadian clock employs a transcriptional/translational negative feedback loop (TTFL) to anticipate environmental changes due to the Earth′s diurnal cycle, with regulation of organismal physiology believed to stem from temporal transcriptional activation by the positive arm. However, up to 80% of oscillating proteins do not have rhythmic mRNA,...
Article
Full-text available
TIMELESS (TIM) was first identified as a molecular cog in the Drosophila circadian clock. Almost three decades of investigations have resulted in an insightful model describing the critical role of Drosophila TIM (dTIM) in circadian timekeeping in insects, including its function in mediating light entrainment and temperature compensation of the mol...
Article
Full-text available
Drosophila suzukii, or spotted-wing drosophila, is now an established pest in many parts of the world, causing significant damage to numerous fruit crop industries. Native to East Asia, D. suzukii infestations started in the United States (U.S.) a decade ago, occupying a wide range of climates. To better understand invasion ecology of this pest, kn...
Article
Full-text available
Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) also known as spotted-wing drosophila (SWD), is a pest native to Southeast Asia. In the last few decades, the pest has expanded its range to affect all major European and American fruit production regions. SWD is a highly adaptive insect that is able to disperse, survive, and flourish under a...
Article
Full-text available
The integration of circadian and metabolic signals is essential for maintaining robust circadian rhythms and ensuring efficient metabolism and energy use. Using Drosophila as an animal model, we show that cellular protein O-GlcNAcylation exhibits robust 24-hour rhythm and represents a key post-translational mechanism that regulates circadian physio...
Preprint
Full-text available
Drosophila suzukii, or spotted-wing drosophila, is now an established pest in many parts of the world, causing significant damage to numerous fruit crop industries. Native to East Asia, D. suzukii infestations started in the United States a decade ago, occupying a wide range of climates. To better understand invasion ecology of this pest, knowledge...
Article
Full-text available
Studying how novel phenotypes originate and evolve is fundamental to the field of evolutionary biology as it allows us to understand how organismal diversity is generated and maintained. However, determining the basis of novel phenotypes is challenging as it involves orchestrated changes at multiple biological levels. Here, we aim to overcome this...
Article
Chrysodeixis includens (Walker, [1858]) is one of the most important defoliator of soybean in Brazil because of its extensive geographical distribution and high tolerance to insecticides compared with other species of caterpillars. Because of this, we conducted bioassays to evaluate the efficacy of pyrethroid λ-cyhalothrin on a C. includens resista...
Article
Full-text available
Circadian clocks orchestrate daily rhythms in organismal physiology and behavior to promote optimal performance and fitness. In Drosophila, key pacemaker proteins PERIOD (PER) and TIMELESS (TIM) are progressively phosphorylated to perform phase-specific functions. Whereas PER phosphorylation has been extensively studied, systematic analysis of site...
Preprint
Full-text available
Studying how novel phenotypes originate and evolve is fundamental to the field of evolutionary biology as it allows us to understand how organismal diversity is generated and maintained. However, determining the basis of novel phenotypes is challenging as it involves orchestrated changes at multiple biological levels. Here, we aim to overcome this...
Article
Full-text available
Background: One hypothesis for the function of sleep is that it serves as a mechanism to conserve energy. Recent studies have suggested that increased sleep can be an adaptive mechanism to improve survival under food deprivation in Drosophila melanogaster. To test the generality of this hypothesis, we compared sleep and its plastic response to sta...
Preprint
Full-text available
The integration of circadian and metabolic signals is essential for maintaining robust circadian rhythms and ensuring efficient metabolism and energy use. Using Drosophila as an animal model, we showed that cellular protein O-linked N-acetylglucosaminylation (O-GlcNAcylation) exhibits robust 24-hour rhythm and is a key post-translational mechanism...
Chapter
This book presents the current knowledge on arthropod vision and the results of successful manipulations of arthropods. It also suggests new methods for using optical manipulation to protect against arthropod pests and to improve the performance of beneficial arthropods. Due to the applied nature of this book, only brief overviews of the knowledge...
Article
Full-text available
Significance Extracting information regarding calendar time from seasonal changes in photoperiod and temperature is critical for organisms to maintain annual cycles in physiology and behavior. Here we found that, in flies, EYES ABSENT (EYA) protein acts as a seasonal sensor by adjusting its abundance and phase in response to changes in photoperiod...
Preprint
Full-text available
Circadian clocks orchestrate daily rhythms in organismal physiology and behavior to promote optimal performance and fitness. In Drosophila , key pacemaker proteins PERIOD (PER) and TIMELESS (TIM) are progressively phosphorylated to perform phase-specific functions. Whereas PER phosphorylation has been extensively studied, systematic analysis of sit...
Preprint
Full-text available
Organisms possess photoperiodic timing mechanisms to anticipate variations in day length and temperature as the seasons progress. The nature of the molecular mechanisms interpreting and signaling these environmental changes to elicit downstream neuroendocrine and physiological responses are just starting to emerge. Here, we demonstrate that in Dros...
Chapter
This book introduces transcriptomics and presents an array of its uses in entomology, past and present. It starts with a thorough introduction to how transcriptomics works, its history, and some of its broad-stroke uses in insect science (Chapter 1). This chapter is followed by an exhaustively comprehensive look at how transcriptomics analysis is p...
Article
Full-text available
Circadian rhythms are generated by endogenous pacemakers that rely on transcriptional-translational feedback mechanisms conserved among species. In Drosophila, the stability of a key pacemaker protein PERIOD (PER) is tightly controlled by changes in phosphorylation status. A number of molecular players have been implicated in PER destabilization by...
Article
Full-text available
Circadian clocks control daily rhythms in behavior and physiology. In Drosophila, the small ventral lateral neurons (sLNvs) expressing PIGMENT DISPERSING FACTOR (PDF) are the master pacemaker neurons generating locomotor rhythms. Despite the importance of sLNvs and PDF in circadian behavior, little is known about factors that control sLNvs maintena...
Data
1 Appendix Figure S1. Dom RNAi efficiency. 2 Appendix Figure S2. PER expression pattern in other groups of circadian neurons. 3 Appendix Figure S3. DOM-A, but not DOM-B was clk dependent. 4 Appendix Figure S4. DomA and domB locomotor activity. 5 Appendix Figure S5. Eliminations of dorsal projections in DOM and DOM-A downregulation were due to re...
Article
Full-text available
Tuta absoluta is one of the most devastating pests of fresh market and processing tomatoes. Native to South America, its detection was confined to that continent until 2006 when it was identified in Spain. It has now spread to almost every continent, threatening countries whose economies rely heavily on tomatoes. This insect causes damage to all de...
Article
Full-text available
Background In the summer of 2013, Aedes aegypti Linnaeus was first detected in three cities in central California (Clovis, Madera and Menlo Park). It has now been detected in multiple locations in central and southern CA as far south as San Diego and Imperial Counties. A number of published reports suggest that CA populations have been established...
Article
Full-text available
Circadian clocks coordinate time-of-day-specific metabolic and physiological processes to maximize organismal performance and fitness. In addition to light and temperature, which are regarded as strong zeitgebers for circadian clock entrainment, metabolic input has now emerged as an important signal for clock entrainment and modulation. Circadian c...
Data
Workflow in analyzing PER PTMs using wper0; FLAG-per(WT) flies. (A) Western blot showing 15N PER from fly heads collected at ZT 12 subjected to immunoprecipitation using FLAG resin followed by acetone precipitation. (B) Western blot probing for immunoprecipitated PER from 14N fly heads using FLAG antibody at the indicated time-points in LD conditio...
Data
Blocking PER(S942) O-GlcNAcylation promotes PER-CLK interaction and leads to premature CLK removal at per and tim promoters. (A) Western blot showing a different biological replicate of PER and CLK reciprocal CoIPs from adult fly heads collected at the indicated time-points on LD3. Protein extracts from fly heads were directly analyzed (input) or i...
Data
Blocking PER(S942) O-GlcNAcylation does not influence the timing of PER nuclear translocation. (A) Representative confocal images (four sets shown for each genotype per time-point) obtained in the sLNv neurons of adult fly brains collected at the indicated time-points on the third day of LD. PER was visualized using α-HA (3F10) antibodies (stained...
Data
Chemoenzymatic O-GlcNAc-labeling approach to detect O-GlcNAc modified PER and OGT in Drosophila S2 cells. (A) Western blot showing O-GlcNAc modified and non-O-GlcNAcylated PER-V5 from S2 cell extracts. Protein extracts from S2 cells were directly analyzed by western blotting (input) or subjected to immunoprecipitation using α-V5 resin. Purified PER...
Data
CAFE assay to examine daily feeding activity rhythms of flies entrained together with flies used for O-GlcNAc chemoenzymatic labeling experiments shown in Fig 6. Feeding rhythms of (A) mixed populations of male and female wper0; p{per(WT)-HA10HIS} flies (n = 3) or (B) male or female flies housed separately over a 24-hour cycle as measured by CAFE a...
Data
Identification of PER phosphorylation sites in fly tissues by label-free mass spectrometry. (DOCX)
Data
Assessing molecular and behavioral rhythms in 14N and 15N-labeled flies. (A) Eduction graphs showing the average locomotor activity of 14N and 15N-labeled flies on the indicated days in LD condition or in constant darkness (DD). (B) Double-plot actograms showing average locomotor activity of 14N and 15N-labeled flies entrained for four days of LD c...
Data
Assaying non-O-GlcNAcylatable per mutant variants in Drosophila S2 cell culture. (A) Western blot showing the expression of different PER variants and CLK in S2 cells for a second replicate of per-luc assay. HSP70 was used for normalization. (B) Quantification of PER expression in the per-luc assay from two biological replicates (shown in S3 Fig. a...
Data
Cycling clock gene expression and PER abundance in per(S951A/T952/T954A) mutant flies. (A,B) Steady state mRNA expression of per and tim in heads of wper0; p{per(WT)-HA10HIS}, and wper0; p{per(S951A/T952A/T954A)-HA10HIS} flies, entrained in 12h:12h LD condition and assayed on LD3 (A) or DD1 (B) (n = 3 biological replicates). Error bars indicate ± S...
Data
Blocking PER(S951/T952/T954) O-GlcNAcylation does not significantly influence PER-CLK interaction. (A) Representative western blots showing CLK and PER reciprocal coIPs in wper0; p{per(WT)-HA10HIS} and wper0; p{per(S951A/T952A/T954A)-HA10HIS} flies at the indicated time-points. Flies were entrained for 2 days in 12h:12h LD cycles and collected on L...
Data
Mutagenic primer sequences to generate PER O-GlcNAc site mutants. (DOCX)
Article
Full-text available
The animal circadian timing system interprets environmental time cues and internal metabolic status to orchestrate circadian rhythms of physiology, allowing animals to perform necessary tasks in a time-of-day-dependent manner. Normal progression of circadian rhythms is dependent on the daily cycling of core transcriptional factors that make up cell...
Article
Full-text available
Root nodule symbiosis (RNS) is a symbiotic interaction established between angiosperm hosts and nitrogen-fixing soil bacteria in specialized organs called root nodules. The host plants provide photosynthate and the microsymbionts supply fixed nitrogen. The origin of RNS represents a major evolutionary event in the angiosperms, and understanding the...
Preprint
Full-text available
In the summer of 2013, Aedes aegypti Linnaeus was first detected in three cities in central California (Clovis, Madera and Menlo Park). It has now been detected in multiple locations in central and southern CA as far south as San Diego and Imperial Counties. A number of published reports suggest that CA populations have been established from multip...
Preprint
Full-text available
Circadian clocks control daily rhythms in physiology. In Drosophila, the small ventral lateral neurons (sLNvs) expressing PIGMENT DISPERSING FACTOR (PDF) are the master pacemaker neurons. Despite the importance of sLNvs and PDF in circadian behavior, little is known about factors that control sLNvs maintenance and PDF accumulation. Here, we identif...
Article
Maternally transmitted Wolbachia, Spiroplasma, and Cardinium bacteria are common in insects [1], but their interspecific spread is poorly understood. Endosymbionts can spread rapidly within host species by manipulating host reproduction, as typified by the global spread of wRi Wolbachia observed in Drosophila simulans [2, 3]. However, because Wolba...
Article
Monarch butterflies are best known from their migratory North American range, although many resident, year-round breeding populations are established throughout the world. Here, we evaluate two non-exclusive hypotheses for the loss of migration in resident monarch populations: (1) absence of cues that trigger migration; and (2) loss of sensory, neu...