Joana Silveira SoaresUniversity of Porto | UP · CIIMAR - Interdisciplinary Centre of Marine and Environmental Research
Joana Silveira Soares
Contracted Researcher
About
27
Publications
4,739
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
476
Citations
Introduction
J Soares obtained her PhD in Biomedical Sciences in 2014. She is a young researcher with a solid background in ecotoxicology and genomics, as well as a strong experience in the biology and endocrinology of vertebrates. Her research focuses on the hazard assessment of endocrine disruptors and the correlation with their underlying mechanisms of action, centred in the chemicals’ effects on reproduction and embryonic development. The teleost fish Danio rerio has been her main animal model. Overall, her research is based in an integrated analysis of endpoints for a more accurate evaluation of contaminants effects and risk of exposure (embryotoxicity, behaviour analysis, molecular biomarkers).
Additional affiliations
March 2019 - present
February 2018 - December 2018
Position
- PostDoc Position
Description
- Evaluate the potential hazard of mining activities in deep-sea taxa and/or proxy species, simulating deep-sea conditions.
November 2016 - November 2017
Education
January 2007 - April 2014
September 2000 - March 2005
Institute of Biomedical Sciences Abel Salazar, University of Porto
Field of study
- Aquatic Sciences
Publications
Publications (27)
Although chemicals are usually present in the environment in complex mixtures, ecotoxicological risk assessments often rely on data from single exposures to contaminants. The present study aimed at examining the effects of two ubiquitous endocrine disrupting chemicals (EDCs), 17α-ethynilestradiol (EE2) and tributyltin (TBT), single and combined, in...
Industrial deep-sea mining will release plumes containing metals that may disperse over long distances; however, there is no general understanding of metal effects on marine ecosystems. Thus, we conducted a systematic review in search of models of metal effects on aquatic biota with the future perspective to support Environmental Risk Assessment (E...
Due to non-linear interactions, the effects of contaminant mixtures on aquatic ecosystems are difficult to assess, especially under temperature rise that will likely exacerbate the complexity of the responses. Yet, under the current climatic crisis, assessing the effects of water contaminants and temperature is paramount to understanding the biolog...
The foreseen rise on maritime transportation of Hazardous and Noxious Substances (HNS) increases the likelihood of accidents, leading to a higher risk of chemical spillage that can have severe ecological impacts. Considering the lack of information on HNS spills, the response to these events is less well established than those involving oil. Moreov...
The deep-sea is the biggest ecosystem in the world and is characterized by extreme conditions such as high pressure, low temperatures and absence or limited light. Despite the scarce studies due to inaccessibility, these ecosystems are considered highly biodiverse. The deep-sea is subjected to anthropogenic stressors with deep-sea mining being a li...
The report summarises the current scientific, technical and legal challenges of deep sea mining debated by international and Portuguese experts and stakeholders at a conference held in Porto, Portugal, on 16th April 2018. The discussions focused on the different obstacles to deep sea mining and how to address them, including recommendations for fut...
Simvastatin (SIM), a hypocholesterolaemic compound, is among the most prescribed pharmaceuticals for cardiovascular disease prevention worldwide. Several studies have shown that acute exposure to SIM causes multiple adverse effects in aquatic organisms. However, uncertainties still remain regarding the chronic effects of SIM in aquatic ecosystems....
Simvastatin (SIM), a hypocholesterolaemic compound, is among the most prescribed pharmaceuticals for cardiovascular disease prevention worldwide. Several studies have shown that acute exposure to SIM is able to produce multiple adverse effects in aquatic organisms. However, uncertainties still remain regarding the chronic effects of SIM in aquatic...
Hazardous and Noxious Substances (HNS) are defined as any substance other than oil, which if introduced into the marine environment is likely to create hazards to human health, to harm living resources and other marine life, as well as to damage amenities and/or to interfere with other legitimate uses of the sea (IMO, 2000). Due to their diverse ph...
Hazardous and Noxious Substances (HNS) are defined as any substance other than oil, which if introduced into the marine environment is likely to create hazards to human health, to harm living resources and other marine life, as well as to damage amenities and/or to interfere with other legitimate uses of the sea (IMO, 2000). Due to their diverse ph...
Recent findings indicate that different Endocrine Disrupting Chemicals (EDCs) interfere with lipid metabolic pathways in mammals and promote fat accumulation, a previously unknown site of action for these compounds. The antifoulant and environmental pollutant tributyltin (TBT), which causes imposex in gastropod snails, induces an "obesogenic" pheno...
Principal Component Analysis of the brain gene expression profile in TBT-exposed zebrafish.
(PDF)
TBT concentration in the water.
Evaluation of the concentration of Tributyltin (TBT), Dibutyltin (DBT) and Monobutyltin (MBT) in the water from the 50 ng/L TBT (as Sn) exposure tanks at 20 days post fertilization. The aquaria were dosed twice daily (at 0 and 8 hours).
(PDF)
Primer pair sequences used for RT-qPCR analysis.
(PDF)
Principal Component Analysis of the liver gene expression profile in TBT-exposed zebrafish.
(PDF)
Hepatic organotin levels.
(PDF)
Visceral fat in TBT exposed zebrafish.
(PDF)
Quantification of organotins in zebrafish liver.
(PDF)
Zebrafish mortality.
Mortality rate in zebrafish exposed to TBT (as Sn) at 60 days post fertilization (dpf).
(PDF)
Both field and experimental data examined the influence of exposure to environmental contaminant
tributyltin (TBT) on marine organisms. Although most attention focused on the
imposex phenomenon in gastropods, adverse effects were also observed in other taxonomic
groups. It has been shown that imposex induction involves modulation of retinoid signal...
Clofibric acid (CA) is an active metabolite of The blood lipid lowering agent clofibrate, a pharmaceutical designed to work as agonist of peroxisome proliferator-activated receptor alpha (PPARa). It is The most commonly reported fibrate in aquatic environments with low degradation rate and potential environmental persistence. Previous fish exposure...
Parental full life-cycle exposure to ethinylestradiol (EE₂) significantly affects embryo development and survival. One of the possible mechanisms of action of EE₂ may involve the impairment of an organism's ability to repair DNA damage. DNA repair mechanisms have sophistically evolved to overcome DNA damaging hazards that threaten the integrity of...
Whereas biochemical and molecular parameters have been well recognised as important “signposts” of individual disturbance to endocrine disrupting chemical’s (EDCs) exposure, behavioural endpoints are yet greatly overlooked as a routine tool in environmental risk assessment of EDCs. However, life histories are intimately associated with numerous int...
Exposure of fish to the synthetic estrogen ethinylestradiol (EE2) has been shown to induce a large set of deleterious effects. In addition to the negative impact of EE2 in reproductive endpoints, concern has recently increased on the potential effects of EE2 in fish embryonic development. Therefore, the present study aimed at examining the effects...
Questions
Questions (2)
I am performing biomarkers analysis in samples of mussels' digestive glands. I homogenize the tissue in phosphate buffer [100 mM (K2HPO4/KH2PO4), 150 mM KCl, 1 mM DTT, 0.1 mM PMSF, 1 mM ethylenediaminetetraacetic acid (EDTA); pH 7.4]. Following protein determination (Lowry method) we perform aliquots (100 µl) of the homogenate and use them to determine GST, SOD and CAT activity as well as lipid peroxidation.
We aim to evaluate metallothioneins (MT) but we don´t have any standardize protocol at our lab. I found some papers for MT analyses by spectrophotometry, but the homogenizing protocol is different from the one that we use. Does anyone know if we can use our material (aliquots obtained from the above described method and used for the other biomarkers) for MT analysis? D you have knowledge of a protocol for microplate reader analysis?
Thank you very much.
Hello,
According to FET protocol (OECD TG 236, 2013) 20 embryos (1 per well) is an adequate number for LC50 calculation. Embryos are treated as independent replicates. The same as been corroborate by a validation study to the OECD protocol. This protocol is not directly proposed to analyse zebrafish embryo developmental anomalies, which is the objective of my work. In these context, I have the doubt if 20 embryos are a sufficient n, if they are really independent replicates and what kind of statistical analysis should I performed. Can anyone specialized in FET help me with these doubts. Thank you very much.
Joana
