Jitao Zhang

Michigan State University | MSU · Department of Biomedical Engineering

Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biol...

Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biol...

Congenital scoliosis (CS) is a type of vertebral malformation whose etiology remains elusive. The notochord is pivotal for vertebrae development but its role in CS is still understudied. Zebrafish knockout of ptk7a, a planar cell polarity (PCP) gene that is essential for convergence and extension (C&E) of the notochord, developed congenital scolios...

Urinary collecting tubules form during kidney embryogenesis through the branching of the ureteric bud epithelium. A travelling mesenchyme niche of nephron progenitor cells caps each branching ureteric bud tip. These ‘tip domain’ niches pack more closely over developmental time and their number relates to nephron endowment at birth. Yet, how the cro...

Osteocytes’ response to dynamic loading plays a crucial role in regulating the bone mass but quickly becomes saturated such that downstream induction of bone formation plateaus. The underlying mechanisms that downregulate osteocytes’ sensitivity and overall response to loading remain unknown. In other cell types, purinergic signaling through the P2...

Cellular biomechanics plays critical roles in cancer metastasis and tumor progression. Existing studies on cancer cell biomechanics are mostly conducted in flat 2D conditions, where cells behavior can differ considerably from those in 3D physiological environments. Despite great advances in developing 3D in vitro models, probing cellular elasticity...

Neurulation is a highly synchronized biomechanical process leading to the formation of the brain and spinal cord, and its failure leads to neural tube defects (NTDs). Although we are rapidly learning the genetic mechanisms underlying NTDs, the biomechanical aspects are largely unknown. To understand the correlation between NTDs and tissue stiffness...

Background: Epithelial-mesenchymal transition (EMT) is a biological process where epithelial cells lose their adhesive properties and gain invasive, metastatic, and mesenchymal properties. Maintaining the balance between the epithelial and mesenchymal stage is essential for tissue homeostasis. Many of the genes promoting mesenchymal transformation...

Background: Epithelial-mesenchymal transition (EMT) is a biological process where epithelial cells lose their adhesive properties and gain invasive, metastatic, and mesenchymal properties. Maintaining the balance between epithelial and mesenchymal stage is essential for tissue homeo-stasis. Many of the genes promoting mesenchymal transformation has...

Multimodal optical techniques are useful for the comprehensive characterization of material properties. In this work, we developed a new, to the best of our knowledge, multimodal technology that can simultaneously measure a subset of mechanical, optical, and acoustical properties of the sample and is based on the integration of Brillouin (Br) and p...

To elucidate the physiological, cellular, and molecular mechanisms responsible for initiating and sustaining ocular neuropathic pain, we created a blue-light-exposure model in C57BL/6 mice. Mice were exposed to 12 h of blue or white light followed by 12 h of darkness. Before blue light exposure, baseline tear secretion, stability, and ocular hypera...

Brillouin microscopy based on spontaneous Brillouin scattering has emerged as a unique elastography technique because of its merit of non-contact, label-free, and high-resolution mechanical imaging of biological cell and tissue. Recently, several new optical modalities based on stimulated Brillouin scattering have been developed for biomechanical r...

Multimodal optical imaging techniques are useful for various applications, including imaging biological samples for providing comprehensive material properties. In this work, we developed a new modality that can measure a set of mechanical, optical, and acoustical properties of a sample at microscopic resolution, which is based on the integration o...

Brillouin microscopy is a technique for mechanical characterization of biological material without contact at high three-dimensional resolution. Here, we introduce dual line-scanning Brillouin microscopy (dLSBM), which improves acquisition speed and reduces irradiation dose by more than one order of magnitude with selective illumination and single-...

Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed an optical modality that c...

Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed a new optical modality tha...

Brillouin microscopy is a promising all-optical technique for biomechanics but is limited by slow acquisition speed and/or large irradiation doses. Here, we introduce multiplexed Brillouin microscopy that overcomes both these limits by over one order of magnitude with selective illumination and single-shot analysis of hundreds of points along the i...

To understand the dynamics of tissue stiffness during neural tube formation and closure in a murine model, we have developed a multimodal, coaligned imaging system combining optical coherence tomography (OCT) and Brillouin microscopy. Brillouin microscopy can map the longitudinal modulus of tissue but cannot provide structural images. Thus, it is l...

Confocal Brillouin microscopy is an emerging tool for biomechanics and biophysics but encounters challenges of slow speed and redundant illumination. We developed a multiplexing configuration that is ten times faster and has much less phototoxicity.

During cancer metastasis, tumor cells undergo significant deformation in order to traverse through endothelial cell junctions in the walls of blood vessels. As cells pass through narrow gaps, smaller than the nuclear diameter, the spatial configuration of chromatin must change along with the distribution of nuclear enzymes. Nuclear stiffness is an...

Several techniques have been developed over the past few decades to assess the mechanical properties of biological samples, which has fueled a rapid growth in the fields of biophysics, bioengineering, and mechanobiology. In this context, Brillouin optical spectroscopy has long been known as an intriguing modality for noncontact material characteriz...

How migrating cells differentially adapt and respond to extracellular track geometries remains unknown. Using intravital imaging, we demonstrate that invading cells exhibit dorsoventral (top-to-bottom) polarity in vivo. To investigate the impact of dorsoventral polarity on cell locomotion through different confining geometries, we fabricated microc...

In article number 1907688, Vivek B. Shenoy, Giuliano Scarcelli, and co‐workers use all‐optical Brillouin microscopy and 3D chemomechanical modeling to investigate the regulation of nuclear mechanics. It is observed that the nuclear modulus can be modulated by both epigenetic regulation targeting internal nanostructures and cytoskeletal behavior thr...

The mechanical properties of the cellular nucleus are extensively studied as they play a critical role in important processes, such as cell migration, gene transcription, and stem cell differentiation. While the mechanical properties of the isolated nucleus have been tested, there is a lack of measurements about the mechanical behavior of the nucle...

Assessing the biomechanical properties of the crystalline lens can provide crucial information for diagnosing disease and guiding precision therapeutic interventions. Existing noninvasive methods have been limited to global measurements. Here, we demonstrate the quantitative assessment of the elasticity of crystalline lens with a multimodal optical...

Terahertz (THz) spectroscopy with high sensitivity is essential for biological application considering the strong absorption and scattering effects therein. As the most commonly used THz detector, the photoconductive antenna’s (PCA) response greatly relies on the properties of the substrate’s material. THz detection properties of the PCAs fabricate...

The alteration of biomechanical properties of many tissues could indicate onset and progression of diseases. In this paper we combine dynamic optical coherence elastography with 3D Brillouin Spectroscopy for truly quantitative elastography of ocular tissues.

The alternation of biomechanical properties of many tissues could indicate onset and progression of diseases. In this paper we combine dynamic Optical Coherence Elastography with 3D Bruillion Spectroscopy for truly quantitative elastography of ocular tissues.

The terahertz (THz) radiation properties of a photoconductive antenna (PCA) fabricated on a GaAs-on-sapphire (GoS) substrate are reported at sub-THz band. The GaAs layer with a thickness of approximately 1 µm was directly deposited on a sapphire wafer by means of molecular beam epitaxy. A butterfly-shaped antenna structure was then fabricated on th...

Background: Embryonic development involves the interplay of driving forces that shape the tissue and the mechanical resistance that the tissue offers in response. While increasing evidence has suggested the crucial role of physical mechanisms underlying embryo development, tissue biomechanics is not well understood because of the lack of techniques...

Photoconductive antenna (PCA) is one of the most widely used terahertz (THz) devices nowadays. Although PCAs have been extensively studied through both theoretical analysis and device design, there still lacks a common agreement upon the mechanism of THz radiation. One of the central questions is how to distinguish and assess the contribution of th...

In the past decades, there has been increased awareness that mechanical properties of tissues and cells are closely associated with disease physiology and pathology. Recognizing this importance, Brillouin spectroscopy instrumentation, already utilized in physics and material science, has been adopted for cell and tissue biomechanics. For biomedical...

Embryogenesis is regulated by numerous changes in mechanical properties of the cellular microenvironment. Thus, studying embryonic mechanophysiology can provide a more thorough perspective of embryonic development, potentially improving early detection of congenital abnormalities as well as evaluating and developing therapeutic interventions. A num...

Embryos undergo dramatic changes in size, shape, and mechanical properties during development, which is regulated by both genetic and environmental factors. Quantifying mechanical properties of different embryonic tissues may represent good metrics for the embryonic health and proper development. Alternations and structure coupled with biomechanica...

The mechanical properties of the nucleus are closely related to many cellular functions; thus, measuring nuclear mechanical properties is crucial to our understanding of cell biomechanics and could lead to intrinsic biophysical contrast mechanisms to classify cells. Although many technologies have been developed to characterize cell stiffness, they...

We developed a label-free flow cytometry platform to characterize the nuclear mechanics based on Brillouin microscopy, which is an all-optical technique so that can measure the stiffness of the nucleus in a non-contact, non-invasive manner.

Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this wo...

Brillouin imaging of turbid biological tissues requires an effective rejection of the background noise due to elastic scattering of probe laser light. We have developed a narrowband spectral notch filter based on a pair of a free-space Fabry-Perot etalon and a mirror. The etalon filter in a 4-pass configuration is able to suppress elastically-scatt...

Photoconductive antenna (PCA) array based THz near-field imager incorporating Hadamard multiplexing method is developed in this work. By using a 2 × 2 dipole antenna array as the THz transmitter, the system signal-to-noise ratio (SNR) is demonstrated to be improved by a factor of 2 as the theory predicts. Additionally, a 2-D scanning of a metallic...

Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouinspectrometers do not have sufficient spectralextinction to reject the predominant non-B...

Brillouin spectroscopy allows non-invasive measurement of the mechanical properties of a sample by measuring the spectra of acoustically induced light scattering therein, and thus has been widely investigated for biomedical application. Recently, the development of fast Brillouin spectrometry based on virtually-imaged phased array (VIPA) has made i...

Brillouin microscopy has recently emerged as powerful technique to characterize the mechanical properties of biological tissue, cell and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Br...

We report a novel configuration of a two-stage virtually imaged phased array spectrometer that enables high-throughput sub-GHz spectroscopy at a high finesse ( > 750 ). Two etalons with different free spectral range and different dispersion are arranged in an orthogonal direction and spread the spectrum across two dimensions, with a greatly improve...

In this work, a 2 × 2 photoconductive antenna (PCA) array is used in a THz near field imaging setup as THz emitters while the sample is placed close to the antenna array (the antenna-sample distance is about 10 μm). A microlens array is used to couple and focus femto-second laser pulse onto each antenna. The response of a sample of gold pattern on...

In this work, butterfly shaped photoconductive antennas (PCAs) on low-temperature grown (LT) GaAs and semi-insulating (SI) GaAs substrate as terahertz (THz) emitters are experimentally characterized and compared. Dependences of the THz radiated field on the applied DC bias voltage and laser pump power are compared. Quadratic DC bias dependence is s...

Photoconductive antennas (PCA) (one of the most commonly used terahertz emitters) have limited performance (e.g. low output power) and as such, do not fully meet the increasing needs of applications. This work attempts to find a route to further improve the performance of PCA by studying the dependence of emission properties on various parameters....

Improving terahertz(THz) radiation power and/or optics-to-THz efficiency of the photoconductive antenna(PCA) is widely recognized as one of the most attractive and challenging missions in THz community. In this work, the design of a THz PCA with nano-crossfinger structure in the active region is proposed. The THz radiation properties of this PCA wa...

The emission properties of terahertz(THz) photoconductive antenna (PCA) have been numerically studied by three-dimensional finite-difference-time-domain method based on the full-wave model. The dependence of the THz radiation on various parameters, such as laser power, bias voltage, substrate's material, pulse duration of the laser, beam spot's siz...

The enhanced terahertz radiation from a new photoconductive antenna (PCA) has been predicted by numerical simulation. Different from the conventional PCA, the proposed PCA has electrodes with sawtooth structures on the edge, which will introduce stronger localized bias field than common electrode (e.g. strip-line structure). In addition, the ellipt...

A numerical model of terahertz (THz) photoconductive antenna (PCA) is developed and studied. This model includes the multi-physical phenomena of the PCA involving the light-matter interaction, photo-excited carrier dynamics and EM propagation of the generated THz radiation. Both in-house finite-difference-time-domain (FDTD) method and commercial to...

We numerically describe the physical mechanism underlying the terahertz photoconductive antenna (PCA) by the finite-difference time-domain method in three-dimension. The feature of our approach is that the multi-physical phenomena happening in the PCA, such as light-matter interaction, photo-excited carrier dynamics and full-wave propagation of the...