Jirí Novák

Jirí Novák
Masaryk University | MUNI · Research Centre for Toxic Compounds in the Environment

PhD

About

40
Publications
9,229
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1,111
Citations

Publications

Publications (40)
Article
Retinoids are newly detected compounds in aquatic ecosystems associated with cyanobacterial water blooms. Their potential health risks are only scarcely described despite numerous detections of all-trans retinoic acid (ATRA) and its derivatives in the environment. Besides the known teratogen ATRA there is only little or no information about their p...
Article
Pollution of indoor environment, where people spend much of their time, comprises complex mixtures of compounds with vastly understudied hazard potential. This study examined several important specific toxic effects and pollutant levels (177 compounds) of indoor samples (air gas phase, PM10 and dust) from different microenvironments after two extra...
Poster
Full-text available
Assessment of the potential endocrine disruptive effects of dust samples from several types of indoor environment, in two extracts with different polarities
Poster
Full-text available
A poster summarizing the main findings from the study focused on the molecular mechanisms of air pollutant mixtures. Graphical summary of the recently published paper compiled for the annual student conference held at Masaryk University in May 2020.
Article
Full-text available
Background Air pollution, which represents a major environmental risk to human health, comprises a complex mixture of compounds where only little is known about its specific toxicities. Objectives This study examined the specific toxicities associated with ambient air pollutant mixtures with respect to gas/particle partitioning, particulate matter...
Article
Inhalation exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with various adverse health effects, including chronic lung diseases and cancer. Using human bronchial epithelial cell line HBE1, we investigated the effects of structurally different PAHs on tissue homeostatic processes, namely gap junctional intercellular communi...
Article
Ambient air pollution, namely exposure to air particulate matter (PM), has been shown to be connected with a number of adverse health effects. At least part of the effects can be caused by organic pollutant mixtures associated with PM, which can elicit a wide range of specific toxic potentials. These potentials could be affected by seasonal variati...
Article
Full-text available
Among the nitrated and oxygenated PAHs (NPAHs, OPAHs) are some of the most hazardous substances to the public health, mainly due to their carcinogenicity and oxidative potential. Despite these concerns the concentrations and fate of NPAHs and OPAHs in the atmospheric environment are largely unknown. Ambient air concentrations of 18 NPAHs, 5 quinone...
Article
Full-text available
Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell...
Article
Complex mixtures of contaminants from multiple sources, including agriculture, industry or wastewater enter aquatic environments and might pose hazards or risks to humans or wildlife. Targeted analyses of a few priority substances provide limited information about water quality. In this study, a combined chemical and effect screening of water quali...
Poster
Full-text available
EU commission Water Framework Directive considers employment of passive sampling and use of effect-based tools in the monitoring of aquatic pollution. A combination of both approaches was used for monitoring of two rivers differing significantly in pollution levels. The Bosna, moderate-sized river in Bosnia-Herzegovina, which is burdened by untreat...
Article
Many aquatic pollutants can be present at low concentrations, but their mixtures can still affect health or behavior of exposed organisms. In this study, toxicological and chemical analyses were combined for spatial contamination profiling using an innovative passive sampling approach. A novel Dynamic Passive Sampler (DPS) was employed as a mobile...
Article
A "dynamic" passive sampling (DPS) device, consisting of an electrically driven large volume water pumping device coupled to a passive sampler exposure cell, was designed to enhance the sampling rate of trace organic compounds. The purpose of enhancing the sampling rate was to achieve sufficient method sensitivity, when the period available for sam...
Article
Full-text available
Chemicals in the environment occur in mixtures rather than as individual entities. Environmental quality monitoring thus faces the challenge to comprehensively assess a multitude of contaminants and potential adverse effects. Effect-based methods have been suggested as complements to chemical analytical characterisation of complex pollution pattern...
Article
Surface waters can contain a diverse range of organic pollutants, including pesticides, pharmaceuticals and industrial compounds. While bioassays have been used for water quality monitoring, there is limited knowledge regarding the effects of individual micropollutants and their relationship to the overall mixture effect in water samples. In this s...
Article
Surface waters can contain a range of micropollutants from point sources, such as wastewater effluent, and diffuse sources, such as agriculture. Characterizing the source of micropollutants is important for reducing their burden and thus mitigating adverse effects on aquatic ecosystems. In this study, chemical analysis and bioanalysis were applied...
Article
Complex mixtures of micropollutants, including pesticides, pharmaceuticals and industrial chemicals emitted by wastewater effluents to European rivers may compromise the quality of these water resources and may pose a risk to ecosystem health and abstraction of drinking water. In the present study, an integrated analytical and bioanalytical approac...
Poster
Full-text available
Many components of complex organic pollutant mixtures in waters are often present at very low levels, posing a challenge for current analytical methods. Passive sampling is one of the perspective methods that could address this problem. In our work, we used a mobile ”active” passive sampling system with two types of sorbent (EMPORE discs, silicone...
Article
This paper provides a brief overview of alternative methods in acute systemic toxicity and focuses on their position in the modern human health risk assessment. Current traditional toxicity testing strategies rely primarily on the observation of adverse health responses in homogeneous groups of animals exposed to high doses of a test agent. However...
Article
Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effect of complex chemical mixtures. In this study, bioassays indicative of activation of the a...
Chapter
Full-text available
Organic pollutants are often present in the water column at trace concentrations that are difficult to detect when conventional low volume spot sampling of water is applied. The scope of the sampling campaign performed using passive samplers was the screening of trace organic pollutants and their toxic potentials in the water column of the Danube,...
Article
Full-text available
Although passive sampling has been previously used for the monitoring of volatile and semi-volatile contaminants in air, there are limited data on the use of this technique coupled with bioassays based on specific biological responses. Biological responses including those mediated by the aryl hydrocarbon (AhR) receptor as well as (anti-)estrogenici...
Data
Exposure to particulate matter (PM) in ambient air has been shown to lead to adverse health consequences. Six size fractions of PM with aerodynamic diameter smaller than 10 μm (PM 10) and gas phase were collected at six localities with different major pollution sources. Extracts of samples were assessed for AhR-mediated toxicity, (anti-)estrogenici...
Article
Chemicals in air were characterized for potential interference with signaling of estrogen, androgen, and arylhydrocarbon (AhR) receptors, which are known to play an important role in endocrine-disruptive changes in vivo. Previously, effects of this type have been studied mainly in particulate matter in the ambient air from various localities. In th...
Poster
Male reproductive function in animals and humans is considered to be a system highly sensitive to many chemicals and physical agents generated by industrial or agricultural activities [1]. Recently, many disturbing trends have been observed in male fertility, such as decreasing sperm counts, deteriorating semen quality and increasing frequencies of...
Chapter
Full-text available
Levels and sources, mass size and phase distributions of parent PAHs and the toxicity of ambient aerosols at urban and rural sites of central (Czech Republic) and south-eastern (Bosnia and Hercegovina) Europe, from 2006 to 2008, are investigated. PAH pollution levels are much higher in winter than in summer, obviously due to the seasonalities of em...
Article
Full-text available
Pollution of surface soils by traffic, especially along major highways, can be a significant issue. Numerous studies have demonstrated traffic to be an important source of particulate matter and gas-phase organic air pollutants that produce many types of deleterious effects. This article brings original information about the presence of contaminant...
Article
Full-text available
Traditionally, contamination of air has been evaluated primarily by chemical analyses of indicator contaminants and these studies have focused mainly on compounds associated with particulates. Some reports have shown that air contaminants can produce specific biological effects such as toxicity mediated by the aryl hydrocarbon receptor (AhR) or mod...
Article
Although the assessment of circulatory levels of retinoids has become a widely used biomarker of exposure to environmental pollutants, the adverse effects caused by imbalance of the retinoid metabolism and signaling in wildlife are not known in detail. Retinoids play an important role in controlling such vital processes as morphogenesis, developmen...
Article
Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, such as malformations in frogs or changes in metabolism of birds, could be related to disruption of the retinoid signaling pathway by exposure to organic contaminants. A new reporter gene assay has been established for evaluati...

Questions

Question (1)
Question
We are looking for a simple system of documentation of cell cultures (passage number, origin, growth characteristics...) and their batches in LN. Full featured LIMS would be too complex (and too expensive) for us. Documentation evidence in excel is not sufficient any more. Do you have any suggestions? 

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Projects

Projects (4)
Project
Breaking down the wall between human health and environmental testing of endocrine disrupters: EndocRine Guideline Optimisation
Archived project