Jiri Hrdy

Jiri Hrdy
Charles University in Prague | CUNI

About

46
Publications
5,166
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388
Citations
Citations since 2016
34 Research Items
331 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080

Publications

Publications (46)
Article
Full-text available
Live biotherapeutic products constitute an emerging therapeutic approach to prevent or treat inflammatory bowel diseases. Lactobacillus acidophilus is a constituent of the human microbiota with probiotic potential, that is illustrated by improvement of intestinal inflammation and antimicrobial activity against several pathogens. In this study, we e...
Article
Toxocara canis, a gastrointestinal parasite of canids, is also highly prevalent in many paratenic hosts, such as mice and humans. As with many other helminths, the infection is associated with immunomodulatory effects, which could affect other inflammatory conditions including autoimmune and allergic diseases. Here, we investigated the effect of To...
Article
Full-text available
Early postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocuous environmental antigens and compounds of normal...
Article
Full-text available
This article reviews the literature on SARS-CoV-2 pandemic and multiple sclerosis (MS). The first part of the paper focuses on the current data on immunopathology of SARS-CoV-2 and leading vaccines produced against COVID-19 infection. In the second part of the article, we discuss the effect of Disease Modifying Therapies (DMTs) on COVID-19 infectio...
Preprint
Full-text available
Live biotherapeutic products constitute an emerging therapeutic approach to prevent or treat inflammatory bowel diseases. Lactobacillus acidophilus is a constituent of the human microbiota with probiotic potential, that are illustrated by direct and indirect antimicrobial activity against several pathogens and improvement of intestinal inflammation...
Article
Full-text available
Understanding the early events involved in the induction of immune tolerance to harmless environmental antigens and microbiota compounds could reveal potential targets for allergic disease therapy or prevention. Regulatory T cells (Treg), particularly induced Treg (iTreg), are crucial for the induction and maintenance of tolerance against environme...
Chapter
Full-text available
Streptomycetes, typical soil dwellers, can be detected as common colonizers of human bodies, especially the skin, the respiratory tract, the guts and the genital tract using molecular techniques. However, their clinical manifestations and isolations are rare. Recently they were discussed as possible “coaches” of the human immune system in connectio...
Article
Full-text available
Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides...
Article
Full-text available
Current treatment of chronic diseases includes, among others, application of cytokines, monoclonal antibodies, cellular therapies, and immunostimulants. As all the underlying mechanisms of a particular diseases are not always fully clarified, treatment can be inefficient and associated with various, sometimes serious, side effects. Small secondary...
Article
Full-text available
Crohn’s disease is linked to a decreased diversity in gut microbiota composition as a potential consequence of an impaired anti-microbial response and an altered polarization of T helper cells. Here, we evaluated the immunomodulatory properties of two potential probiotic strains, namely a Bifidobacterium animalis spp. lactis Bl 5764 and a Lactobaci...
Chapter
Human milk is a unique and complex secretion differing from lacteal secretions of other species. Besides nutrition, it provides protection during the newborn's adaption to the extrauterine environment and reduces the morbidity and mortality caused by both infectious and noninfectious diseases. Its components act directly against infectious agents,...
Article
Aims: Trichobilharzia regenti (Schistosomatidae) percutaneously infects birds and mammals and invades their central nervous system (CNS). Here we characterised the peripheral immune response of infected mice and showed how it was influenced by the parasite-induced inflammation in the skin and the CNS. Methods and results: As revealed by flow cyt...
Article
Full-text available
Streptomycetes, typical soil dwellers, can be detected as common colonizers of human bodies, especially the skin, the respiratory tract, the guts and the genital tract using molecular techniques. However, their clinical manifestations and isolations are rare. Recently they were discussed as possible "coaches" of the human immune system in connectio...
Article
Objective: Review of current knowledge about particular neutrophil subsets and their role in preeclampsia. Design: Review. Setting: Institute of Immunology and Microbiology, First Faculty of Medicine Charles University and General University Hospital in Prague. Introduction: Preeclampsia represents one of the major complications of pregnancy...
Article
Full-text available
Allergic diseases represent some of the most common immunological disorders with high clinical and economic impact. Despite intensive research, there are still few universally accepted and reliable biomarkers capable of predicting their development at an early age. There is therefore a pressing need for identification of potential predictive facto...
Article
Continuous increasing incidence of allergic diseases is calling for identifying early prognostic markers pointing to increased risk of allergy development and establishing protocols for preventive strategies limiting allergy development in predisposed individuals. It is important to better understand the critical events occurring in early postnatal...
Article
Full-text available
Aim: To assess the systemic and local immunological response to subcutaneous implants of a vascular graft covered with collagen extracted from the European carp (freshwater fish) or with collagen of bovine origin. Methods: Pieces of a vascular graft covered by pure bovine (Bos taurus, BOV, n=14) or carp (Cyprinus carpio, CYP, n=14) collagen 5 mm...
Article
Due to the increasing incidence of allergic diseases, there is a strong need to identify a prognostic marker pointing to increased risk of allergy development allowing introduction of early preventive measures. Cord blood seems to be a good source for searching for such marker. The capacity of cord blood cells to respond to common allergens could p...
Article
Full-text available
Abstract Cercarial dermatitis (CD) is an allergic skin disease that rises in consequence of infection by invasive stages (cercariae) of trematodes of the family Schistosomatidae. CD has been considered a re-emerging disease, human cases have been reported from all continents, and tourism-threatening outbreaks occur even in frequented recreational a...
Article
Full-text available
Allergic diseases represent a major issue in clinical and experimental immunology due to their high and increasing incidence worldwide. Allergy status of the mother remains the best predictor of an individual’s increased risk of allergy development. Dysregulation of the balance between different branches of immune response, chiefly excessive polari...
Data
Suppression of proliferation of CFSE-stained non-Treg cells. CD4+CD25-CD127+ target cells were magnetically isolated from cord blood mononuclear cells (n = 19), stained with 5 μM CFSE and cocultured with CD4+CD25+CD127low Treg cells at 1:5 Treg:target cell ratio. After 72 hours, cells were harvested, stained for CD4 and analysed by flow cytometry....
Data
Summary table of data from CFSE-based suppression assays. CD4+CD25-CD127+ target cells were magnetically isolated from cord blood mononuclear cells (n = 19), stained with 5 μM CFSE and cocultured with CD4+CD25+CD127low Treg cells at 1:5 Treg:target cell ratio. After 72 hours, cells were harvested, stained for CD4 and analysed by flow cytometry. Tab...
Data
Gating strategy, FMO controls and representative dot plots of surface and intracellular markers. Cord blood samples were stained and analysed by flow cytometry. A-D: Gating strategy of CD4+CD25+CD127low cells.E-P: FMO controls and representative dot plots of CD4+CD25+CD127low cells stained for surface (E-J) and intracellular (K-P) markers. E, G, I:...
Data
Representative histograms of staining for neuropilin-1. Several whole blood samples were stained for CD4, CD25, neuropilin-1, FoxP3 and Helios and analysed by flow cytometry. A: CD25highFoxP3+ cells were gated into Helios+ and Helios- populations. B: Expression of neuropilin-1 on Helios+ (blue) and Helios- (red) cells. FMO control for neuropilin-1...
Poster
Full-text available
Recently, numerous studies of human microbiome suggest that streptomycetes are common colonizers of human bodies - the respiratory tract, skin, uterus. Their function is unclear, however, some works show correlation of their incidence with chronic inflammatory diseases - psoriasis and chronic obstacle pulmonary disease. Generally, they are viewed a...
Article
Human alveolar echinococcosis (AE) is caused by larval stages of the tapeworm Echinococcus multilocularis. In the Czech Republic, screening tests to detect the specific infectious agent have been performed since 1998. The first AE cases were diagnosed in 2007, and until 2014, a total of 21 diseases were recorded. In accordance with radiological, hi...
Article
A series of well-defined methoxy-poly(ethylene glycol)-block-polylactic acid (mPEG-b-PLA) diblock copolymers were successfully synthesized, characterized and used for the construction of anticancer nanoparticle delivery system. Nanoparticles (NPs) based on these polymers were prepared by employing the nanoprecipitation method, and they were non-cov...
Article
Full-text available
Allergic diseases belong to one of the most common diseases with steadily increasing incidence even among young children. There is an urgent need to identify a prognostic marker pointing to increased risk of allergy development enabling early preventive measures introduction. It has been shown that administration of selected probiotic strains or mi...
Article
Full-text available
Gut microbiota dysbiosis plays a central role in the development and perpetuation of chronic inflammation in inflammatory bowel disease (IBD) and therefore is key target for interventions with high quality and functional probiotics. The local production of stable probiotic formulations at limited cost is considered an advantage as it reduces transp...
Article
Objective: To summarize available data concerning the role of maternal imunity and woman's microbiome in the pathogenesis of preterm labor and their use in clinical practice. Setting: Department of Obstetrics and Gynecology od the First Faculty of Medicine, Charles University in Prague, and General Teaching Hospital. Design: Review article. Methods...
Article
Full-text available
Probiotics are believed to prevent or reduce allergy development but the mechanism of their beneficial effect is still poorly understood. Immune characteristics of regulatory T cells (Tregs) in peripheral blood of perinatally probiotic-supplemented children of allergic mothers (51 children), non-supplemented children of allergic mothers (42 childre...
Article
Bird schistosomes parasitize mammals as non-specific hosts. Neurotropic Trichobilharzia regenti migrates extravasally via nervous tissue in experimentally infected mice. The majority of successfully penetrated larvae remain in the skin; the rest migrate through peripheral nerves to the spinal cord and brain. The potential of schistosomula to leave...
Article
Full-text available
To the Editor: Human alveolar echinococcosis (AE) is a zoonotic parasitic disease caused by larvae of Echinococcus multilocularis tapeworms that manifests most often in the host’s liver, although it can infect the lungs, brain, and other organs. Clinical symptoms usually develop after a long incubation period (5–15 years), causing considerable diag...
Article
The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The matu...
Article
Taxane and platinum-based chemotherapy regimens are standard treatment for advanced ovarian carcinoma. Expression levels of putative markers of taxane resistance in carcinoma tissues and paired peritoneal samples (n=55) and in 16 samples of ovaries without signs of carcinoma were compared with clinical data and the patients´ time to progression. KI...
Article
Full-text available
Allergy is one of the most common diseases with constantly increasing incidence. The identification of prognostic markers pointing to increased risk of allergy development is of importance. Cord blood represents a suitable source of cells for searching for such prognostic markers. In our previous work, we described the increased reactivity of cord...
Article
There is no doubt about the beneficial effect of breastfeeding on the newborn's immune system. It is not fully elucidated what the differences are between the colostrum/milk of healthy and allergic mothers and how beneficial breastfeeding by an allergic mother is. The gene expression of selected cytokines was tested in cells isolated from colostra...
Article
Full-text available
The study investigated possible mechanisms by which second-generation taxanes, established as significantly more effective than paclitaxel in vitro, suppress a rat lymphoma model in vivo. The studied mechanisms included taxane pharmacokinetics, expression of genes dominating their metabolism (Cyp3a1/2) and transport (Abcb1) and genes controlling tu...
Article
The objective is to study the effect of after-birth oral colonization by a probiotic Escherichia coli strain in infants of allergic mothers to reduce occurrence of allergy later in life. In a controlled clinical trial, 158 infants were randomly divided into groups of (i) 56 colonized infants of allergic mothers, (ii) 57 control infants of allergic...
Article
To determine some early signs connected with the increased risk of future allergy development, gene expression and production of selected cytokines were tested in children of allergic mothers and compared with newborns of healthy mothers. Expression of IL-1β, IL-2, IL-4, IL-8, IL-10, IL-13, IFN-γ, TNF-α, TGF-β and EGF was tested in cord blood cells...
Article
s: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Nov 15-19, 2009; Boston, MA Novel taxoids SB‐T‐1214, SB‐T‐12851, SB‐T‐12852, SB‐T‐12853, SB‐T‐12854 and IDN‐5109 proved to be more effective than paclitaxel in vitro in MDA‐MB‐435 cell line, but especially in NCI/ADR‐RES cell line, highly expressing ABCB1. Thus,...
Article
Acyclic nucleoside phosphonates PMEDAP and PMEG modulate expression of selected proangiogenic genes in SD-lymphoma bearing rats. Antiangiogenic efficacy of PMEDAP is relatively weak and is manifested mainly by down-regulation of vascular endothelial growth factor (VEGF) and its receptor VEGFR detectable 24 hours after treatment. Compound PMEG (an a...
Article
Maternal milk has beneficial effects on the development and function of the newborn's immune system. Whether the milk of allergic mother has the same effects as the milk of healthy mothers is not yet quite clear. To contribute to the characterization of its immunomodulatory action, we tested the effect of milk of healthy and allergic mothers on the...

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Projects

Projects (5)
Project
Effective search for producers of novel, pharmaceutically attractive natural compounds requires application of new screening techniques using genomic data. Growing information on the genetics of biosynthesis of natural compounds and their structural components, brings the possibility to use newly characterized enzymatic systems as markers in genetic screening of potential producers of structuraly and functionaly unique active metabolites, which may find application as novel antibiotics, immunomodulators and cancerostatics. Two model enzymes of actinomycetes, the cyclizing aminolevulinate synthase and a novel type of type II polyketide synthase specific for short linear polyketide chains, will be employed in the project. Amis: 1) By means of genetic screening targeting two novel biosynthetic genes to search for potential producers of novel bioactive compounds. 2) To analyze biosynthetic gene clusters, which contain the target genes and identify the encoded compounds. 3) To assay biological activities of new metabolites.
Project
Mucosal immunization is able to induce both mucosal and systemic immune response. My lab previously identified delipidated Bacillus firmus as potent adjuvant able to induce Th1 immune response. I study the effect of DBF on cell populations in murine NALT, especially on Tregs.