Jiří Danihlík

Jiří Danihlík
Palacký University Olomouc · Department of Biochemistry

PhD

About

27
Publications
10,706
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440
Citations
Introduction
Oxidative stress and honey bees Antimicrobial peptides: production and activity Honey bee colony losses in Czechia Varroa IPM in Czechia
Additional affiliations
September 2013 - December 2015
Centre of Region Haná for Biotechnological and Agricultural Research Crop Research Institute
Position
  • Researcher

Publications

Publications (27)
Article
In the temperate climates of middle Europe and North America, two distinct honeybee (Apis mellifera) populations are found in colonies: short-living summer bees emerge in spring and survive until summer, whereas long-living winter bees emerge in late August and overwinter. Besides the difference in their life spans, each of these populations fulfil...
Article
Full-text available
Citation: Pijacek M, Bzdil J, Bedanova I, Danihlik J, Moravkova M (2021): The inhibiting effect of microwave radiation on Paenibacillus larvae spores suspended in water. Vet Med-Czech 66. Abstract: The aim of this paper was to investigate the effects of microwave radiation on the viability of Paeniba-cillus larvae spores and to study the relationsh...
Article
Full-text available
European foulbrood (EFB) is an infectious disease of honey bees caused by the bacterium Melissococcus plutonius. A method for DNA isolation and conventional PCR diagnosis was developed using hive debris, which was non-invasively collected on paper sheets placed on the bottom boards of hives. Field trials utilized 23 honey bee colonies with clinical...
Article
Full-text available
This article presents managed honey bee colony loss rates over winter 2018/19 resulting from using the standardised COLOSS questionnaire in 35 countries (31 in Europe). In total, 28,629 beekeepers supplying valid loss data wintered 738,233 colonies, and reported 29,912 (4.1%, 95% confidence interval (CI) 4.0–4.1%) colonies with unsolvable queen pro...
Article
Full-text available
Honey bees are the most important managed pollinators and provide income because of bee products. In Austria and Czechia, we monitored winter losses of honey bee colonies and also collected information on the apicultural sector, hive management, population dynamics and treatment against the mite Varroa destructor from 2013-14 to 2016-17. Numbers of...
Article
Full-text available
Beekeepers have various options to control the parasitic mite Varroa destructor in honey bee colonies, but no empirical data are available on the methods they apply in practice. We surveyed 28,409 beekeepers maintaining 507,641 colonies in 30 European countries concerning Varroa control methods. The set of 19 different Varroa diagnosis and control...
Article
Full-text available
In temperate climates, honey bee workers of the species Apis mellifera have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive informatio...
Article
The green microalga Chlorella vulgaris Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, Chlorella vulgaris has potential as a pollen supplement. We studied whether Chlorella extracts display antimicrobial properties against Paenibacillus larvae, the causative agent of the honey bee bacte...
Article
Full-text available
American foulbrood (AFB) is a dangerous disease of honeybees (Apis mellifera) caused by the spore-forming bacterium Paenibacillus larvae. According to the ERIC (enterobacterial repetitive intergenic consensus) classification, five genotypes are distinguished, i.e., I, II, III, IV, and V, which differ in their virulence and prevalence in colonies. I...
Article
Full-text available
Honeybees are of great importance because of their role in pollination as well as for hive products. The population of managed colonies fluctuates over time, and recent monitoring reports show different levels of colony losses in many regions and countries. The cause of this kind of loss is a combination of various factors, such as the parasitic mi...
Conference Paper
Full-text available
Honey bees are exposed to too many stress factors that can negatively influence their immune system. Bees of different age have different tasks in the hive and can be also influenced by diverse stress factors. Nurse bees are the very young bees (3-14 days old) and their function in the hive is mainly nursing and feeding the bee larvae. These bees l...
Article
Full-text available
It has been known for many years that in temperate climates the European honey bee, Apis mellifera, exists in the form of two distinct populations within the year, short-living summer bees and long-living winter bees. However, there is only limited knowledge about the basic biochemical markers of winter and summer populations as yet. Nevertheless,...
Article
Full-text available
We tested the effect of Chlorella sorokiniana, a green, unicellular, freshwater alga, provided as a food supplement on several biological characteristics of the honey bee Apis mellifera. Chlorella was applied as (1) a moisturized powder, (2) a sugar-water solution, or (3) mixed with honey-sugar candy. All three applications were well accepted by th...
Article
Full-text available
This short article presents loss rates of honey bee colonies over winter 2017/18 from 36 countries, including 33 in Europe, from data collected using the standardized COLOSS questionnaire. The 25,363 beekeepers supplying data passing consistency checks in total wintered 544,879 colonies, and reported 26,379 (4.8%, 95% CI 4.7–5.0%) colonies with uns...
Article
Full-text available
We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute FeedbeeTM) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms...
Article
Full-text available
In this short note we present comparable loss rates of honey bee colonies during winter 2016/2017 from 27 European countries plus Algeria, Israel and Mexico, obtained with the COLOSS questionnaire. The 14,813 beekeepers providing valid loss data collectively wintered 425,762 colonies, and reported 21,887 (5.1%, 95% confidence interval 5.0–5.3%) colo...
Article
Full-text available
In this short note we present comparable loss rates of honey bee colonies during winter 2016/2017 from 27 European countries plus Algeria, Israel and Mexico, obtained with the COLOSS questionnaire. The 14,813 beekeepers providing valid loss data collectively wintered 425,762 colonies, and reported 21,887 (5.1%, 95% confidence interval 5.0–5.3%) col...
Article
Divergent abiotic stresses induce osmotic stress on plant cells resulting in an imbalance in water homeostasis which is preserved by aquaporins. Since the plasma membrane aquaporins (PIPs) were shown to be involved in seed development and responses to abiotic stresses, we focused on determining the contribution of mannitol-induced osmotic stress, b...
Article
Full-text available
In this short note we present comparable loss rates of honey bee colonies during winter 2015/16 from 29 countries, obtained with the COLOSS questionnaire. Altogether, we received valid answers from 19,952 beekeepers. These beekeepers collectively wintered 421,238 colonies, and reported 18,587 colonies with unsolvable queen problems and 32,048 dead...
Article
Full-text available
Honey bee immune responses are composed of a complex suit of individual immune mechanisms and special types of behavioral adaptations. The main focus of this paper is innate immunity in the honey bee, and specifically, the role and function of antimicrobial peptides (AMPs). Insect innate immunity constitutes evolutionary conserved defense strategie...
Data
Losses of honey bee colonies over the 2013/14 winter Preliminary results from an international study The honey bee protection network COLOSS1 has today announced the preliminary results of an international study to investigate honey bee winter colony losses. Data were collected from Israel and Algeria and 19 European countries. In total 17,135 resp...

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Projects

Projects (3)
Project
Honey bees are of great importance. In this project we aim for a standardized evaluation of colony losses in several countries. This builds up a database on colony mortality that allows risk analysis. http://coloss.org/coreprojects/monitoring Since 2014 this COLOSS core project is co-chaired by Robert Brodschneider (robert.brodschneider@uni-graz.at) and Alison Gray (a.j.gray@strath.ac.uk)