Jingkuan Wang

• Doctor of Philosophy
• Professor (Full) at Shenyang Agricultural University

Understanding the mechanisms of converting plant residue carbon (C) into soil C is important for managing the soil C pool and improving soil fertility. However, little is known yet about how the heterogeneous C from the plant residues, e.g., from the various plant parts, is bound in the aggregates of soil with different initial fertility. To addres...

While long-term plastic film mulching (LFM) of farmland can improve the yield and quality of crops, it also poses ecological risks through the accumulation of microplastics (MPs) in soil and alterations in soil nitrogen (N) cycling. However, no systematic studies (based on long-term experiments) have studied the effects of LFM on both soil MPs accu...

Soybean continuous cropping could alter soil microbial communities, leading to the development of continuous-cropping obstacles that negatively impacted yield. Different soybean cultivars exhibited varying degrees of resistance to these obstacles. However, the mechanisms underlying this resistance remain unclear. In this study, microbiology and met...

Aims The aim of this study was to investigate the effects of varying nitrogen (N) and carbon (C) levels on soil phosphorus (P) compositions, abiotic factors (pH and moisture content), and biotic factors (dehydrogenase (DHA) and phosphatases). Methods A field experiment was conducted at the Inner Mongolia prairie, where different levels of N (0, 25...

Straw returning may stimulate soil microbial activity, thereby influencing microbial-mediated soil nitrification, which can lead to nitrate leaching and nitrogen (N) loss. However, its effects under long-term nitrogen fertilization remain unclear. At an experimental station with 34 years of fertilizer application (0, 135, and 270 kg ha⁻¹ N), we inv...

In their Letter‐to‐Editor, Liu & Chen (2024) raised the importance and suitability of our recent stoichiometric approach to assess the contribution of plant residue (litter) and microbial sources to mineral‐associated organic matter (MAOM). We highly appreciate their Letter as well as the further suggestion and comments and offer two points of clar...

Purpose The multiple benefits of biochar use as a soil amendment has garnered global attention. Biochar addition is a crucial factor to improve soil biomass, soil enzyme activities, microbial biomass and improve soil nutrient utilization rate. However, the precise mechanism of effects of biochar addition on microbial community structure and diversi...

【目的】农用地膜表面是塑料降解菌筛选、动植物病原菌定殖及抗生素抗性基因富集的潜在热点区域，但地膜表面招募微生物的群落基本特征仍不明确，一定程度上限制了我们对地膜表面微生物定殖过程的理解。【方法】本研究基于地膜覆盖和施氮长期定位试验，2021年秋季采集覆膜当季的地膜样品，利用高通量测序分析了地膜表面的细菌与真菌群落特征。 【结果】地膜表面细菌以蓝细菌门（Cyanobacteria）、变形菌门（Proteobacteria）、 放线菌门（Actinobacteria）为主，而真菌以座囊菌纲（Dothideomycetes）为主。地膜及土壤表面均产生肉眼可见的绿色絮状微生物菌落，这说明了覆膜对地表微气候的改善有利于地衣系统的发育。施氮间接提高微生物群落α多样性，但不改变β多样性。同时，与对照相比，...

Aims The degradation and transformation of soil organic nitrogen (SON) in semi-arid steppe are regulated by a series of enzymes involved in nitrogen(N) hydrolysis, the influence of N and carbon (C) additions on the soil N reserves, activities of N-hydrolyzing enzymes, and their relationships remain unclear. Methods In the Inner Mongolia prairie of...

Mollisols region of China is a ballast stone of Chinese food security. In this study, 484 soil organic carbon (SOC) data points from the documented soil surface (0–20 cm) samples in the typical Chinese Mollisols region were selected. The regional soil fertility was divided into high, medium, and low levels based on the SOC content from the long‐ter...

Purpose Microbial necromass carbon (C) is a major contributor to soil organic C (SOC) sequestration. Soil aggregates are key functional units in regulating SOC dynamics. Fertilization and plastic film mulching, as common agricultural management practices, affect soil aggregation and microbial activities. However, how microbial necromass C is accumu...

Straw return with nitrogen (N) fertilizer can generally regulate soil N cycling by affecting the microbial community, thereby influencing crop productivity. Bacteria and fungi play a key role in nitrification, while the effects of soil microbial interactions that include protists under the condition of straw return and N fertilizer remain uncertain...

Introduction Ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) have been proven to be key microorganisms driving the ammonia oxidation process. However, under different fertilization practices, there is a lack of research on the impact of interaction between predators and AOA or AOB on nitrogen cycling at the multi-trophic level....

Mineral-associated soil organic matter (MAOM) is the largest, slowest-cycling pool of carbon (C) in the terrestrial biosphere. MAOM is primarily derived from plant and microbial sources, yet the relative contributions of these two sources to MAOM remain unresolved. Resolving this issue is essential for managing and modeling soil carbon responses to...

This study aimed to explore how the yield, water use efficiency (WUE), and physiological characteristics of greenhouse-cultivated tomatoes in Northeast China respond to different water and nitrogen treatments during the flowering to fruit set stage (Stage 1), fruiting stage (Stage 2), and ripening stage (Stage 3). Three irrigating upper and lower l...

Microbial necromass carbon (MNC) is a major contributor to soil organic carbon (SOC) sequestration. Fertilization combined with plastic film mulching, as an intensive agricultural practice to increase crop yields, affects soil microbial growth and metabolism. Nevertheless, how fertilization combined with mulching affects SOC sequestration by mediat...

Background and aims Crop straw and it-derived biochar both increase soil fertility and stabilize soil structure. The contribution of these amendments to microbial biomass and aggregate formation, as well as carbon (C) and nitrogen (N) stabilization within aggregates is lacking. Methods Maize straw that was labelled with 13C and 15N or its biochar (...

Soil physical structure plays a key role in the sequestration of organic carbon (C) and affects soil fertility and global climate change. However, the dynamics of the residue-derived C in different aggregate fractions are poorly understood. The objectives of this study were to explore the changes in soil aggregate composition and organic C distribu...

Accurate quantification of evapotranspiration (ETc) and its components are critical for enhancing water use efficiency and implementing precision irrigation. A two-year experiment was conducted for greenhouse-grown tomatoes under mulched drip irrigation with three irrigation treatments during 2020–2021 in Northeast China. Three different irrigation...

Plant inputs and their subsequent microbial transformations drive the formation and accumulation of soil organic carbon (SOC). Rice paddy is more conducive to SOC accumulation than uplands, primarily because of predominant anaerobic conditions. However, the role of microbes and plants in the buildup of SOC under prolonged rice cultivation has not b...

Plastic film mulching and urea nitrogen fertilization are widely used in agricultural ecosystems, but both their long-term use may leave a negative legacy on crop growth, due to deleterious effects of plastic and microplastic accumulation and acidification in soil, respectively. Here, we stopped covering soil with a plastic film in an experimental...

Conventional plastic mulch brings agronomic and economic benefits to crop production, but a large amount of plastic waste amasses when the mulch is removed from the fields after harvest. Soil-biodegradable plastic mulch (BDM) has emerged as a promising alternative to conventional plastic mulch as it can be tilled into the soil after harvest, thereb...

Plastic film mulching (PFM) is critical for agricultural planting and maximizing production in semiarid and arid areas. Particulate organic matter (POM) is assumed to be a sensitive indicator for evaluating the effects of different agricultural practices on soil fertility and the soil organic carbon (SOC) pool. Soil aggregates have the function of...

Ammonia oxidation is the rate-limiting step of the global nitrogen cycle, and it is mainly catalyzed by ammonia-oxidizing bacteria (AOB) and archaea (AOA). AOA can carry out nitrification even in acidic soils, whereas AOB activity was not consistently observed in acidic soils. In this study, we manipulated two environmental variables, i.e., the mic...

Three irrigation treatments were set up in northeast China to investigate soil water movement and root water uptake of greenhouse tomatoes, and the collected experimental data were simulated by HYDRUS-2D. The computation and partitioning of evapotranspiration data into soil evaporation and crop transpiration was carried out with the double-crop coe...

东北黑土地是世界主要黑土带之一，是我国粮食作物主产区，在保障国家粮食安全方面贡献极大。近几十年，东北黑土地出现的“变薄、变瘦、变硬”等耕地质量退化问题严重制约了粮食产能的稳步提升。对东北黑土地资源进行合理、科学的认知，并采取有效措施对黑土地进行保护与利用成为该地区农业可持续发展的关键。然而，东北黑土地在地理位置、自然环境、农业生产和土壤资源组成的复杂性，阻碍了黑土地保护利用政策的科学制定和顺利实施。以此为背景，本文根据东北黑土地自然资源条件和农业生产特征，按照区别差异性、归纳共同性的原则，将东北黑土地划分成松嫩平原区、三江平原区、辽河平原区、长白山辽东区、西部风沙区和大小兴安岭区 6 个类型区，分别概述了不同类型区在气候、地理和土壤组成方面的特征；从气候、农业生产和土壤限制因素等层面探讨了不...

Mean residence time (MRT) of carbon (C) in soil is the most important parameter of C sequestration and stability and crucial for CO2 removal from the atmosphere. Climate and soil properties controls of MRT of upland soils are well known, but the drivers of C stability in paddies were never summarized. Here, we estimated MRT of paddies across monsoo...

Purpose Plant inputs provide the main food and energy for soil organisms. Considering substantial aboveground litter on the soil surface but few belowground input in the non-growing season, we hypothesize that aboveground input would have an important role in sustaining microbial abundance and community at this period. Methods We initialed a manipu...

Soil organic carbon (SOC) accumulation is easily susceptible to tillage managements, which strongly affect soil structure and microbial community structure. Subsoiling is developed for mitigating soil compaction and thus improving soil structure. Coupled with straw residue incorporation, it is expected not only to enhance this improvement but also...

Introduction Soybean continuous cropping will change soil microorganisms and cause continuous cropping obstacles, resulting in a significant yield decline. Different soybean cultivars have different tolerances to continuous cropping, but the relationship between continuous cropping tolerance and soil microorganisms is not clear. Methods Two soybea...

In this study, β-CD@mesoporous SiO2 nanospheres (β-CD@mSi) were prepared by loading β-cyclodextrin (β-CD) onto mesoporous silica nanospheres through an in situ synthesis. This not only solved the defect of β-CD being easily soluble in water, but also changed the physical structure of the mesoporous silica nanospheres. FTIR and XPS results showed th...

Elucidating the physiological mechanisms underlying crop responses to water and nitrogen availability can help optimize the irrigation and fertilization of greenhouse−cultivated tomatoes. This study aimed to determine how photosynthetic and transpiration characteristics and water use of greenhouse−cultivated tomatoes in Northeast China respond to w...

Abstract Soil microorganisms are critical for soil carbon (C) cycling. They primarily regulate the turnover of the soil organic C (SOC) by adjusting their community structure, and contributing residues with a considerable amount to the resistant SOC. Nevertheless, how long-term fertilization (e.g., the combination of manure and chemical fertilizer)...

Surface potential is a key electrochemical property of colloid in the study of particle interactions. However, the specific ion effects are not involved in the existing measuring methods. In this study, approaches to the estimation of the surface potential of montmorillonite with or without consideration of the specific ion effects were explored th...

Nitrogen (N) cycling microorganisms mediate soil nitrogen transformation processes, thereby affecting agricultural production and environment quality. However, it is not fully understood how active N-cycling microbial community in soil respond to long-term fertilization, as well as which microorganisms regulate soil nitrogen cycling in agricultural...

Microbial necromass is the vital component of stable soil organic C (SOC). However, how crop root vs. shoot residue incorporation regulates microbial necromass accumulation within aggregate fractions remains unclear, especially under different soil fertility conditions. Thus, we conducted a 500-day in situ field experiment using low- and high-ferti...

Plant residue is an important carbon (C) source for soil communities that motivates underground C cycling. However, the effects of plant residue quantity on the structure of soil micro‐food web and the transformation of exogenous C within the micro‐food web after increasing organic input are not known. Thus, a microcosm experiment was carried out t...

Purpose Understanding how anions affect the colloidal behavior of clay mineral is greatly important in determining soil particle dispersibility. The surface of soil particles, especially for silicate clay mineral components, is often net negatively charged. However, how anions influence soil particle interactions and affect soil structure has not b...

Input of plant residue carbon (C) stimulates microbial growth and activity, and thus may alter native soil organic matter (SOM) mineralization. The partition of plant residue C between microbial growth and respiration, and priming effect on soil organic C (SOC) are affected by initial SOM levels and plant residue types. However, how the interaction...

The return of crop residues to the soil is a common agricultural management practice for nutrient recycling and carbon sequestration. It is known that nitrogen (N) fertilization can influence crop residue decomposition and nutrient release. However, it is unclear whether the effect of N fertilization interact with plastic film mulching (PFM) or res...

Brown earth is one of the typical soils in the dryland areas of Northeast China, and its degradation is closely related to food security in the local. Effectively preventing soil nitrogen (N) loss can promote the soil fertility supply. As the hub of nitrogen cycling, microorganisms play an important role in N transformation and accumulation. Soil a...

Poly-γ-glutamic acid (γ-PGA) is regarded as a promising fertilizer synergist in agricultural system and plays an important role in soil nitrogen (N) cycle. Amide N fertilizer such as CO(NH2)2 and ammonium N fertilizer such as (NH4)2SO4 have different N turnover in soil. However, how the fate of CO(NH2)2 and (NH4)2SO4 in plant-soil systems would be...

The Mollisol region of Northeast China has a large soil organic carbon (SOC) storage which is important for maintaining soil fertility. SOC is susceptible to various environmental factors; however, the responses of SOC content to environmental factors in different soil layers of cropland remain unclear, particularly in deep soil layers. In this stu...

The variable role and fate of soil colloids under different environmental conditions are derived from their dispersion and aggregation properties. In this work, dynamic and static light scattering were used to characterize the original size, aggregation kinetics of natural black soil colloids (BSCs) and structural features of aggregates in electrol...

“黑土地保护”作为一项国家战略，已经写进我国“十四五”规划和“2035 年远景目标纲要”，中国科学院与东北三省政府签署了“黑土粮仓”科技会战框架协议，全力开展“黑土地保护”科技会战。当前“黑土地保护”科技攻关主要关注提升土壤有机质、降低土壤侵蚀、减少化肥和农药面源污染、推广保护性耕作等热点问题，而黑土地地膜残留与污染已经日渐严重，需要引起社会各界的高度关注和重视。尤其当前“黑土地保护”科技会战中，不能忽视东北地膜碎片（粒径大于 5 mm）及微塑料（小于 5 mm）的残留与污染问题。本文从东北地区地膜应用及污染概况、地膜残留与污染的影响因素、地膜残留对黑土地农业的危害进行了综述，并提出黑土地地膜污染研究亟待开展的研究方向：（1）摸清东北黑土地地膜残留的分布和数量；（2）明确残膜对作物/蔬菜等农...

Plastic film mulch (PFM) is a double-edged-sword agricultural technology, which greatly improves global agricultural production but can also cause severe plastic pollution of the environment. Here, we characterized and quantified the amount of macro- and micro-plastics accumulated after 32 years of continuous plastic mulch film use in an agricultur...

Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) influence the nitrate (NO3⁻) reduction and its final fate in the environment. However, it is unclear how denitrifying and DNRA bacterial communities respond to different environment conditions. Here, we investigated the effects of fertilization and sampling time (April, July and...

Nitrogen (N) fertilization and plastic film mulching (PFM) are two widely applied management practices for crop production. Both of them impact soil organic matter individually, but their interactive effects as well as the underlying mechanisms are unknown. Soils from a 28-year field experiment with maize monoculture under three levels of N fertili...

Soil organic carbon (SOC) has significant implications in regulating soil health. Emerging insights emphasize the important role of microbial anabolism in SOC storage by continuously transforming plant fragments into persistent microbial residues. However, knowledge of the sequestration pathway of root versus shoot carbon (C) is under debate. While...

The subsoiling tillage managements have been recommended to remediate subsoil compaction and maintain crop production. These practices can improve soil properties and fertility due to soil loosening and straw incorporation, and further affect soil microbial community characteristics. However, the response of soil microbial community structure to su...

Abstract Straw incorporation is an effective measure for increasing soil organic carbon (SOC) thereby improving soil quality and crop productivity. However, quantitative assessments of the transformation and distribution of exogenous carbon (C) in soil aggregates under various field fertilization practices have been lacking. In this study, we colle...

Rice paddies account for ~9% or the world’s cropland area and are characterized by environmental conditions promoting soil organic carbon storage, methane emissions and to a lesser extent nitrous oxide emissions. Here, we synthesize data from 612 sites across 51 countries to estimate global carbon stocks in paddy soils and determine the main factor...

传统塑料地膜覆盖带来巨大经济效益的同时，也引起了严重的土壤退化和污染。可降解地膜代替传统塑料地膜是未来的必然选择。本文针对近年来国内外关于传统地膜覆盖带来的土壤塑料残留和可降解地膜的最新研究进展进行了综述，并总结了该领域一些尚未解决的问题。主要包括：（1） 传统塑料地膜覆盖土壤中塑料的残留数量巨大，覆膜 20 a 的农田中大塑料(粒径大于 5 mm)的残留最高能达到 307.95 kg/hm2，覆膜农田中微塑料的残留也不容忽视，目前亟需制定出统一的土壤微塑料 （粒径小于 5 mm） 残留检测标准。（2） 新型可降解地 膜被认为是解决塑料地膜污染最有效的途径之一，但目前没有考虑到可降解地膜会碎裂成微塑料，可能会危害土壤健康，未来应加强这方面的研究。最后，本文对以上方向进行了展望，并提出可降解地...

Straw residue amendment and fertilization are the key global management strategies for achieving more sustainable agriculture. However, the temporal changes in labile soil organic carbon (SOC) fractions and microbial community (MB) in response to differential long-term fertilization during straw residue decomposition remain unclear. We collected to...

Plastic polyethylene mulch has been widely used in crop production, but also causes environmental pollution if plastic residues accumulate in soil. Biodegradable plastic mulches (BDM) are a potential solution to problems caused by polyethylene mulches, as BDMs are designed be tilled into the soil after the growing season and then biodegrade. Howeve...

Purpose The complete ammonia oxidizers (comammox) capable of catalyzing nitrification, oxidizing ammonia to nitrate, via activity of only one type of microbes were recently discovered which has updated our knowledge of traditional two-step nitrification. The extent of contribution of comammox and canonical ammonia oxidizers including ammonia-oxidiz...

The labile organic carbon (C) pool plays a vital role in soil biogeochemical transformation and can be used as a sensitive indicator of the response of soil quality to agricultural practice. However, little is known about how residue type and soil fertilization affect the incorporation of residue C into labile organic C pools. A 360-day laboratory...

PurposeClay-humic substance complexes play a major role in controlling the mobility of elements and colloids in natural soils. The purpose of the present study is to explore the different reaction mechanisms induced by cations on the humic acid (HA) and montmorillonite (Mt) heteroaggregation and to analyze the binding mechanism of HA-Mt.Materials a...

以沈阳农业大学连续 29a 棕壤长期定位施肥试验为基础,以 13C 标记的玉米植株为试验试材,采用田间原位培养方法和磷脂脂肪酸-稳定同位素示踪联用(PLFA-SIP)技术,研究土壤不同粒级团聚体中微生物群落对残体碳的同化状况,及土壤有机碳的固定这一关键生物地球化学过程.结果表明:植物残体添加显著增加了全土及各粒级团聚体中各微生物群落 PLFAs 含量,其中以真菌 PLFAs 含量增幅最高,细菌中以革兰氏阴性菌含量增幅最高;但不同残体类型并未对全土中各群落 PLFAs 含量产生显著影响.茎叶碳与根茬碳的矿化率无显著差异;根茬碳对总 PLFAs 碳库的贡献是茎叶碳的 3.9 倍,说明根茬碳更有利于通过微生物合成的方式贡献于土壤有机碳库.残体碳占不同微生物群落 PLFAs 碳库的比例以真菌最高,表...

Agronomic practices such as long-term use of plastic film mulching (PFM) and application of organic and inorganic fertilizers have profound effect on soil microbial community. However, there is limited information on the abundance and community structure of ammonia-oxidizers where these practices have been employed. In this study, we investigated s...

Low crop yield in organic farming restricts its popularization, and N deficiency is one of important reasons for the yield decline in organic farming. In this study, the biodegradable plastic mulch (BDP) was introduced into organic farming and its effects on soil N mineralization and crop yield were investigated. Over the two experimental years, ap...

PurposeThe distribution and dynamic of residue-derived carbon after incorporation into soil at different fertility levels have been well documented. However, the distribution of maize residue–derived C in particulate organic C (POC) from different types of organic residues in soils of different fertility levels is poorly understood.Material and met...

PurposeSubsoil has great potential for increasing soil organic carbon (SOC) stock. Soil compaction decreases the content of SOC in the topsoil layer and leads to the formation of a plow pan in subsoil. However, measures for improving poor soil structure mainly concentrate on topsoil. This study aims to understand the effect of subsoiling management...

Crop response to phosphorous (P) application is often erratic in most soil types in the world. In alkaline calcareous soils, P retention and mobilization take place due to precipitation and adsorption. P adsorption isotherms are the powerful tools for assessing optimum P levels for different crops and soils. Laboratory and field studies were carrie...

Seed quality is accessed by appropriate seed germination, seed moisture contents, insect/pathogen infestations and seed vigor. Seed storage conditions are essential to protect from deterioration. In this study, knowledge and practices of wheat and cotton seeds storage were accessed among commercial seed growers (CSGs) and non-commercial seeds growe...

Crop residue incorporation is an effective agricultural management to enhance soil organic carbon and nitrogen (N) sequestration, which inevitably affects the crop N uptake, and consequently the allocation of N in the soil-plant system. However, to what extent the crop residue type and soil fertility moderate the contribution and recovery of maize...

Purpose The distribution and dynamic of residue-derived carbon after incorporation into soil at different fertility levels have been well documented. However, the distribution of maize residue–derived C in particulate organic C (POC) from different types of organic residues in soils of different fertility levels is poorly understood. Material and m...

Microbial transformation of crop residue is the key process of soil organic matter (SOM) formation and mineralization, which determines soil fertility and affects global climate change. However, utilization dynamics of residue-derived carbon (residue C) by various microbial communities is still not well understood, especially under different residu...

The LiDAR technology is a means of urban 3D modeling in recent years, and the extraction of buildings is a key step in urban 3D modeling. In view of the complexity of most airborne LiDAR building point cloud extraction algorithms that need to combine multiple feature parameters, this study proposes a building point cloud extraction method based on...