Jijo Lukose

Jijo Lukose
Manipal Academy of Higher Education | MAHE · Department of Atomic and Molecular Physics (DAMP)

Doctor of Philosophy
Currently working on the Spectroscopy of body fluids for clinical applications

About

30
Publications
3,325
Reads
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165
Citations
Citations since 2016
29 Research Items
164 Citations
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2016201720182019202020212022020406080
2016201720182019202020212022020406080
Introduction
Raman spectroscopy of body fluids, Optical tweezers investigation of blood components, Optical Biosensing
Additional affiliations
December 2010 - May 2011
Central Glass and Ceramics Research Institute
Position
  • project student

Publications

Publications (30)
Article
Full-text available
The dermal penetration of bisphenol-A (BPA) from thermal papers into the human skin is a matter of major health concern due to its extensive use in developing countries like India, one of its largest users in the world. Bisphenol A is widely used in the manufacture of many consumer goods like polycarbonate water bottles, baby bottles, food containe...
Article
Full-text available
Advancements in the field of nanotechnology have resulted in the emergence of a large variety of engineered nanomaterials for innumerable applications. Despite the ubiquitous use of nanomaterials in daily life, concerns regarding the potential toxicity and safety of these materials have also been raised. There is a high demand for assessing the unw...
Article
Full-text available
Hydroxyethyl starch (HES) is a commonly used intravenous fluid in hospital settings. The merits and demerits of its application is still a debatable topic. Investigating the interaction of external agents like intravenous fluids with blood cells is of great significance in clinical environments. Micro-Raman spectroscopy combined with an optical twe...
Article
The outbreak of life-threatening pandemic like COVID necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the nasopharyngeal swab method and subsequent detection of RNA using the real-time polymerase chain reac...
Article
Full-text available
Human saliva can be treated as a pool of biological markers able to reflect on the state of personal health. Recent years have witnessed an increase in the use of optical devices for the analysis of body fluids. Several groups have carried out studies investigating the potential of saliva as a non-invasive and reliable clinical specimen for use in...
Article
Full-text available
Malaria, dengue and leptospirosis are three tropical infectious diseases that present with severe hematological derangement causing significant morbidity and mortality, especially during the seasonal monsoons. During the course of these infectious diseases, circulating red blood cells are imperiled to the direct ill-effects of the infectious pathog...
Article
Full-text available
Introduction Fiber optic probe based in-vivo spectroscopy techniques are fast and highly objective methods for intraoperative diagnoses and minimally invasive surgical interventions for all procedures where endoscopic observations are carried out for cancers of different types. The Raman spectral features provide molecular fingerprint-type informat...
Article
Full-text available
Cardiovascular diseases (CVD) are the major causative factors for high mortality and morbidity in developing and developed nations. The biomarker detection plays a crucial role in the early diagnosis of several non-infectious and life-threatening diseases like CVD and many cancers, which in turn will help in more successful therapy, reducing the mo...
Article
Full-text available
Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high potential in probing biochemical changes in clinical...
Article
The present study explores the application of the micro-Raman spectroscopy technique to discriminate normal and cervicitis condition from cervical malignancy by analyzing the Raman signatures of tissues and plasma samples of the same subjects. The Raman peaks from tissue samples at 1026 cm⁻¹,1298 cm⁻¹ and 1243 cm⁻¹ are attributed to glycogen, fatty...
Article
The transformation of normal cervix to cervicitis as well as to cervical cancer is accompanied with biochemical alterations at cellular level. Laser induced fluorescence (LIF) can reflect those changes either as variations in the fluorescence intensity or as shift in the fluorescence maxima of bio fluorophores present in tissues. The curve resolved...
Article
Full-text available
There is an urgent need to have reliable technologies to diagnose post-coronavirus disease syndrome (PCS), as the number of people affected by COVID-19 and related complications is increasing worldwide. Considering the amount of risks associated with the two chronic lung diseases, asthma and chronic obstructive pulmonary disease (COPD), there is an...
Chapter
Optical techniques are becoming increasingly popular for the analysis of body fluids, particularly so after the onset of the COVID-19 pandemic. Raman spectroscopy has found special significance among them due to its ability to perform label-free investigations of biological solutions with high sensitivity and specificity. The integration of Raman s...
Article
Full-text available
Research activities are in full swing globally to translate the use of saliva as a non-invasive and highly potential specimen for clinical diagnostics, particularly for COVID-19 detection. Being comprised of a pool of biomarkers also enriched with ACE-2 receptors, saliva can provide vital information regarding the state of the human body. Advanceme...
Article
Introduction: Human blood and saliva are increasingly under investigation for the detection of biomarkers for early diagnosis of non-communicable (e.g.cancers) and communicable diseases like COVID-19. Exploring the potential application of human tears, an easily accessible body fluid, for the diagnosis of various diseases is the need of the hour.Ar...
Article
Crystalloid fluids, a subset of intravenous (IV) fluid solutions are commonly used in clinical settings. The influence of these fluids on the functions of blood components are least explored. Raman spectroscopy combined with optical trapping has been widely used to evaluate the impact of external stress agents on red blood cells. The present study...
Article
Introduction: The unprecedented outbreaks of corona virus disease of 2019 (COVID-19) have highlighted the necessity of readily available, reliable, precise, and faster techniques for its detection. Nasopharyngeal swab has been the gold standard for the diagnosis of COVID-19. However, it is not an ideal screening procedure for massive screening as i...
Conference Paper
Investigation of body fluids is of paramount importance since it carries a pool of biological markers, which can reflect the health status of humans. Technological advancements in the field of biophotonics offer highly sensitive, objective and rapid detection of abnormal health conditions from body fluid analysis. Raman spectroscopy has been widely...
Article
Extracellular tonicity has a significant influence on human red blood cell deformation capability. Advancements in the area of laser physics and optical trapping have opened up a plethora of applications for understanding cell structure and dynamics. Here, Raman Tweezers technique was employed to investigate the impact of extracellular tonicity by...
Article
Alcohol is commonly used as sterilizing agent in hospital settings. Alcohol abuse is also associated with numerous medical complications. It influences the coagulation mechanics and erythropoiesis. Optical tweezers are being extensively used in research labs for the trapping as well as manipulation of biological cells. The biochemical investigation...
Article
Full-text available
The use of normal saline for washing red blood cells and treating critically ill patients is a regular medical practice in hospital settings. An optical tweezer in combination with Raman spectroscopy is an analytical tool employed for the investigation of single cell dynamics, thus providing molecular fingerprint of the cell by optically trapping t...
Presentation
Full-text available
The skin is the biggest organ of human organism playing an important physiological function of barrier between the organism and environment. The main skin functions are regulation of the water loss, insulation and thermo-regulation as well as protection of the internal organs from external mechanical impact and penetration of pathogens. In this way...
Article
Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healt...
Article
Surface Plasmon Resonance based sensors have found appreciable application in the biochemical and biological research due to its capability of real time monitoring of biomolecular interactions. In this study, we report the label free detection of low weight biological analytes in real time using a lab-built surface plasmon resonance based instrumen...
Article
We have developed a low-cost surface plasmon resonance (SPR) instrument based on the Kretschmann configuration for biosensing applications. The fabricated instrument is capable of operating in both angular and intensity interrogation schemes. The proposed sensor has proved enormously versatile by detecting a range of analytes with low to high molec...
Article
Full-text available
We report the preparation of mixed self-assembled monolayer of 1-octanethiol and 1,10-decanedithiol on Au thin film with preferential Au(111) surface and their characterization using Raman spectroscopy of cysteine adsorbed on mixed self-assembled monolayer mediated isolated Ag nanoparticles. The self-assembled monolayer characterization has been al...
Article
High refractive index (H.R.I.) tetravalent transition metal ion coordinated with organic co-dopant to form charge transfer complexes into the optical acrylic preform. They have been fabricated with different concentrations of transition metal ions and H.R.I. organic co-dopant and characterized. The improved photo physical properties, thermal, struc...

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Projects

Projects (3)
Project
To probe the biochemical changes associated with cervical cancer development through the blood and cervical tissue spectroscopic techniques.
Project
In vitro analysis of the red cell concentrates storage lesions assessed using optical tweezers in addition to usual biochemical parameters.
Project
The work has focused on the design and development of a surface plasmon resonance instrument (SPR) based on Kretschmann configuration for biosensing applications. A mathematical model based on Fresnel’s reflection equations was developed using MATLAB program package to optimize the SPR experimental parameters. The sensor response towards varying concentrations of low weight biomolecules, glucose and glycerine in water was verified. Real time immobilization of protein, Bovine Serum Albumin on metal surface was also recorded. The sensing instrument also made it possible to monitor streptavidin-biotin interaction in real time in the absence of labeling tags. The developed system was also found effective in the real time, label free detection of waterborne pathogen, Salmonella Typhimurium using antibody attached on sensing surface.