Jianjun Wang

Jianjun Wang
China National Petroleum Corporation | CNPC · TGRI

About

59
Publications
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Introduction

Publications

Publications (59)
Article
As the downstream effector of the target of rapamycin complex 1 (TORC1) signaling pathway, the ribosomal protein S6 kinase (S6K) is an important regulator of insect reproduction, however, the underlying mechanism remains obscure. In this study, a S6K gene, named TcS6K1, was isolated from the red flour beetle, Tribolium castaneum. Analysis of tempor...
Article
Intermittent food scarcity is commonly encountered in all organisms including insects. Although the forkhead box O (FOXO) transcription factor is a well‐known component of signalling cascades related to starvation stress, the detailed regulatory mechanisms during long‐term starvation remain poorly understood in insects. In the present study, using...
Article
Metabolism of insecticides is an energy-consuming process. As an important component of the intracellular energy buffering system, arginine kinase (AK) plays an important role in insect cellular energy homeostasis and environmental stress response, but the involvement of AKs in the response to chemical stressors (insecticides) remains largely unkno...
Article
Background: Both forkhead box O (FOXO) and nuclear factor erythroid-derived 2-like-2 (Nrf2) are key transcription factors related to stress response. While limited studies in mammals and Caenorhabditis elegans revealed interaction between FoxO/DAF-16 and Nrf2/SKN-1, the role of FOXO in metabolic detoxification and regulation of Nrf2-Keap1 signalin...
Article
The sulfuryl transfer reaction catalyzed by cytosolic sulfotransferase (SULT) is one of the major conjugating pathways responsible for the detoxification and subsequent elimination of xenobiotics, however, functional characterization of insect SULTs is still limited. In this study, cDNA encoding a cytosolic sulfotransferase, named TcSULT1, was clon...
Article
The insect voltage-gated sodium channel is the primary target of pyrethroids and novel efficient insecticides such as indoxacarb and metaflumizone. In this study, we cloned and characterized two putative sodium channel genes, TcNav1 and TcNav2, in Tribolium castaneum. The composite TcNav1 and TcNav2 encode a protein of 2045 and 2037 amino acid resi...
Article
Full-text available
Background Short interspersed nuclear elements (SINEs) belong to non-long terminal repeat (non-LTR) retrotransposons, which can mobilize dependent on the help of counterpart long interspersed nuclear elements (LINEs). Although 234 SINEs have been identified so far, only 23 are from insect species (SINEbase: http://sines.eimb.ru/ ). Results Here, f...
Article
The oxidation of methionine (Met) by reactive oxygen species (ROS) causes detrimental effects on the protein functions. Methionine sulfoxide reductase (Msr) is the secondary antioxidant enzyme involved in protein repair, and is divided into two distinct classes, MsrA and MsrB, although the mechanisms underlying the transcriptional regulation of Msr...
Article
BACKGROUND The selective insecticide flonicamid shows highly insecticidal activities against piercing‐sucking insects and has been widely used for the control of Hemipteran insect pests, whereas its effects on Lepidopteran insect pests remain largely unknown. Recently, inward rectifier potassium (Kir) channel has been verified to be a target of flo...
Article
Full-text available
The phosphoarginine-arginine kinase shuttle system plays a critical role in maintaining insect cellular energy homeostasis. Insect molting and metamorphosis are coordinated by fluctuations of the ecdysteroid and juvenile hormone. However, the hormonal regulation of insect arginine kinases remain largely elusive. In this report, we comparatively cha...
Article
Full-text available
Background: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In t...
Article
Full-text available
Besides the function of preventing metamorphosis in insects, the juvenile hormone (JH) plays a role in female reproduction; however, the underlying mechanism is largely unknown. The methoprene-tolerant (Met) protein belongs to a family of basic helix-loop-helix–Per-Arnt-Sim (bHLH-PAS) transcription factors and functions as the JH intracellular rece...
Article
BACKGROUND As the largest transporter gene family in metazoans, the ATP‐binding cassette (ABC) transporters regulate the efflux of a broad spectrum of substrates from the cytoplasm to the outside of the cell. In arthropods, ABC transporters are involved in the phase III of detoxification process, and play important roles in the metabolism and trans...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Article
The AMP-activated protein kinase (AMPK) has important roles in the regulation of energy metabolism, and AMPK activity and its regulation have been the focus of relevant investigations. However, functional characterization of AMPK is still limited in insects. In this study, the full-length cDNA coding AMPKα (TcAMPKα) was isolated from the red flour...
Article
Full-text available
Reversible acetylation of core histones plays an important role in the epigenetic regulation of gene transcription, and is controlled by the action of histone acetyltransferases (HATs) and histone deacetylases (HDACs). While HDACs have been well studied in Drosophila melanogaster, information from insect pests is still limited. In the current study...
Article
While sublethal effects of insecticide on insect development have been widely studied, the underlying mechanisms remain elusive. Our previous studies revealed that sublethal concentrations of chlorantraniliprole significantly increased the juvenile hormone levels and resulted in both prolonged developmental time and reduced fecundity in Chilo suppr...
Article
The anthranilic diamide insecticide chlorantraniliprole was widely used for the controlling of Chilo suppressalis in China. Previous studies have revealed sublethal effects of chlorantraniliprole on the development and reproduction of C. suppressalis. In the present study, a comparative transcriptome analysis was performed to investigate the global...
Article
Full-text available
Background: Helitrons play an important role in shaping eukaryotic genomes due to their ability to transfer horizontally between distantly related species and capture gene fragments during the transposition. However, the mechanisms of horizontal transfer (HT) and the process of gene fragment capturing of Helitrons still remain to be further clarif...
Article
Arginine kinase (AK) plays a critical role in insect energy metabolism and has been proposed to be a potential insecticide target for commercial exploitation. In this study, the full length cDNA encoding a typical group 1 insect AK (FoAK) was isolated from the western flower thrips (WFT), Frankliniella occidentalis (Pergande). Sequence analysis sho...
Article
Glutamate-gated chloride channels (GluCls) mediate fast inhibitory neurotransmission in invertebrate nervous systems, and are of considerable interest in insecticide discovery. The full length cDNA encoding CsGluCl was cloned from the rice stem borer Chilo suppressalis (Walker). Multiple cDNA sequence alignment revealed three variants of CsGluCl ge...
Article
Background: Juvenile hormone (JH) regulates development and reproduction of insects. The sublethal effects of chlorantraniliprole on JH levels and mRNA expression of JH acid methyltransferase gene (CsJHAMT) and farnesyl diphosphate synthase genes (CsFPPS1 and CsFPPS2 ) in Chilo suppressalis (Walker) were investigated. Results: Exposure of sublet...
Article
Background: The rice stem borer, Chilo suppressalis (Walker), is one of the most damaging rice pests in the world. The sublethal effects of chlorantraniliprole on development,reproduction, and mRNA expression levels of vitellogenin gene (CsVg) in C. suppressalis were investigated. Results: Exposure of sublethal concentrations of chlorantranilipr...
Article
Full-text available
Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) are members of a family of tetrameric intracellular Ca(2+)-release channels (CRCs). While it is well known in mammals that RyRs and IP3Rs modulate multiple physiological processes, the roles of these two CRCs in the development and physiology of insects remain poorly unde...
Article
Full-text available
Horizontal transfer (HT) of transposable elements has been recognized to be a major force driving genomic variation and biological innovation of eukaryotic organisms. However, the mechanisms of HT in eukaryotes remain poorly appreciated. The non-autonomous Helitron family, Lep1, has been found to be widespread in lepidopteran species, and showed li...
Article
Ryanodine receptors (RyRs) are a distinct class of intracellular Calcium (Ca(2+) ) release channels. Recent discovery of diamide insecticides has prompted the studies on insect RyRs. However, information about the structure and function of insect RyRs is still limited. In this study, we isolated and characterized a full-length RyR cDNA (named as Nl...
Article
Ryanodine receptors (RyRs) are the targets of novel diamide insecticides. The cotton bollworm, Helicoverpa armigera, is one of the most important cotton pests in the world. In this study, we report the full-length RyR cDNA sequence (named as HaRyR) of H. armigera. The 16,083-bp contiguous sequence encoded 5, 142 amino acid residues, which shares 80...
Conference Paper
When using thermal recovery for the exploitation of heavy oil, the casing is the only channel of steam injection and crude oil extraction. Keeping the casing safe is critical to the operation. The authors conducted low-cycle experiments using strain-controlled material and the MTS809 material testing system. Mechanical property parameters of therma...
Conference Paper
Underground natural gas storage can carry out seasonal peak regulation and provide emergency gas supply to industry; however, its completion casing string is subjected to many undesirable influences by high-pressure gas injection and corrosion environment. Therefore, the bearing capacity and service life of casing string will be reduced. By establi...
Article
The accurate prediction of the ultimate residual strength (URS) (including collapse, burst, and tensile strength) of worn casing is important in the design and safety assessment of casings for the oil and gas industry. Based on the true stress–strain constitutive relationship of P110 steel, this paper establishes a three-dimensional (3D) finite ele...
Article
Voltage-gated sodium channels are the targets of several important classes of synthetic insecticides including pyrethroids, indoxacarb and metaflumizone. The rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), is an important rice insect pest throughout Southeast Asia. In this study, we report the cDNA sequence (named as CmNav) of C...
Conference Paper
The S135 drill pipe occurred failure accident for direction crossing rivers during West-East natural gas transmission. In order to analyze the failure reason, this paper carries on the overall analysis to the drill pipe sample based on the magnetic particle testing, fractography, and metallography, et.al. The analysis discovers that: the fracture h...
Article
Histone acetylation is a vital mechanism for the post-translational modifications of chromatin components. Histone acetyltransferases (HATs) are critical elements that determine histone acetylation and regulate chromatin dynamics and gene expression. While histone acetyltransferases have been well studied in mammals and Drosophila melanogaster, inf...
Article
Full-text available
Ryanodine receptors (RyRs) are the targets of two novel classes of synthetic insecticidal chemicals, phthalic acid diamides and anthranilic diamides. Isolation of full-length RyR cDNAs is a critical step towards the structural and functional characterization of insect RyRs and an understanding of the molecular mechanisms underlying the species sele...
Article
Full-text available
Although more than 120 families of short interspersed nuclear elements (SINEs) have been isolated from the eukaryotic genomes, little is known about SINEs in insects. Here, we characterize three novel SINEs from the cotton bollworm, Helicoverpa armigera. Two of them, HaSE1 and HaSE2, share similar 5' -structure including a tRNA-related region immed...
Data
Phylogenetic analysis using maximum parsimony and Bayesian methods. (A) Maximum parsimony tree for HaSE2 elements in Helicoverpa armigera and similar elements in other insect species. (B) Bayesian tree for HaSE2 elements in Helicoverpa armigera and similar elements in other insect species. (DOC)
Data
Genes found in the flanking sequences of full length HaSE1 and HaSE2. (RTF)
Data
Genes found in the flanking sequences of 5′-truncated HaSE1. (RTF)
Data
Predicted secondary structure of HaSE1. Secondary structure of the tRNA-related region in consensus sequence of HaSE1 was predicted by tRNAscan-SE. (RTF)
Data
Alignments of the identified HaSE3 sequences in Helicoverpa armigera. The sequence on the top line is the consensus sequence of the HaSE3 family. Nucleotides shaded in black are conserved across sequences. (RTF)
Data
Alignment of amino acid sequence of partial COOH-terminal region in 31 RyR isoforms from 26 species. Identical amino acids are shown in black boxes and similar amino acids are highlighted in gray boxes. Gaps have been introduced to permit alignment. Triangles below the alignment indicate unique residues (N4922, N4924, N4935, L4950, L4981, N5013 and...
Data
Alignments of the identified HaSE2 sequences in Helicoverpa armigera. The sequence on the top line is the consensus sequence of the HaSE2 family. Putative flanking direct repeats are indicated in lowercase and boxed. No flanking direct repeats were found in HaSE2.6 and HaSE2.7. Nucleotides shaded in black are conserved across sequences. (RTF)
Data
Primers used for genome walking and PCR amplification of HaSE3 elements. (RTF)
Article
Resistance levels in five field strains of Bemisia tabaci Q-biotype in eastern China to six representative insecticides were determined, and the frequencies of synaptic acetylcholinesterase ace1 mutation (F331W) and para-type voltage gated sodium channel mutations (L925I and T929V) were detected using polymerase chain reaction-based monitoring tech...
Article
Mariner transposons are widespread in eukaryote genomes and have been used as transposon vectors in insect transgenesis. We examined two closely related Helicoverpa species, the cotton bollworm Helicoverpa armigera and corn earworm Helicoverpa zea, for the presence of mariner-like elements (MLEs). Multiple copies of two distinct MLEs, Hamar1 and Ha...
Conference Paper
In order to improve the injection and production rate of salt cavern gas storages, the diameter of completion casing is generally larger than that of oil well. Moreover, the loads capacity of rock salt surrounding completion casing decreases for the creep of salt, inducing the increase of external pressure subjected to the completion casing. It may...
Article
Abamectin resistance was selected in the western flower thrips [Frankliniella occidentalis (Pergande)] under the laboratory conditions, and cross-resistance patterns and possible resistance mechanisms in the abamectin-resistant strain (ABA-R) were investigated. Compared with the susceptible strain (ABA-S), the ABA-R strain displayed 45.5-fold resis...
Article
Full-text available
The piggyBac transposable element, originally discovered in the cabbage looper, Trichoplusia ni, has been used widely in genetic engineering of insects including the pink bollworm, Pectinophora gossypiella, a major lepidopteran pest of cotton. Previously, we identified an intact copy of a piggyBac-like element (PLE) in pink bollworm, designated as...
Article
Abstract Mariner transposons are widespread in eukaryote genomes and have been used as transposon vectors in insect transgenesis. We examined two closely related Helicoverpa species, the cotton bollworm Helicoverpa armigera and corn earworm Helicoverpa zea, for the presence of mariner‐like elements (MLEs). Multiple copies of two distinct MLEs, Hama...
Conference Paper
An improved Gauss-Newton-Marquardt (GNM) algorithm is proposed in the paper for displacement back analysis of casing external loads in salt stratum to optimize the solution based on traditional GNM algorithm. In the improved GNM algorithm, the direct solution method of partial derivative used in stochastic finite element method is adopted to substi...
Conference Paper
Based on the pipeline forces distinguishing features in collapsible loess area, a new Elastic-Plastic Foundation Model (EPFM) analyzing the suspended pipelines forces and deformations is established, which considers axial loads and foundation plastic deformations neglected in other available literatures. At the same time the calculating equations o...
Article
Full-text available
The piggyBac transposable element (TE), originally discovered in the cabbage looper, Trichoplusia ni, has been widely used in insect transgenesis including the red flour beetle Tribolium castaneum. We surveyed piggyBac-like (PLE) sequences in the genome of T. castaneum by homology searches using as queries the diverse PLE sequences that have been d...
Article
Transposable element (TE) display is a method exhibiting the insertion site variations of TE, serving for multiple dominant markers. We report an improved TE display method evaluated by testing inheritance pattern of the TE insertion sites in two different piggyBac-like elements in a lepidopteran insect tobacco budworm. The specificity of the polym...
Article
The piggyBac transposable element (TE), originally discovered in the cabbage looper, Trichoplusia ni, has been widely used in insect transgenesis including the red flour beetle Tribolium castaneum. We surveyed piggyBac-like (PLE) sequences in the genome of T. castaneum by homology searches using as queries the diverse PLE sequences that have been d...

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