Jian-Jun Niu

Jian-Jun Niu
Xiamen University | XMU

Doctor of Medicine
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52
Publications
2,100
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312
Citations

Publications

Publications (52)
Article
Full-text available
Aim: The present study examined the membrane location of cardiolipin antigen in treponemes. Materials & methods: The authors used different methods to disrupt the outer membrane of treponemes, detected the location of the cardiolipin antigen and analyzed the immune response in rabbits immunized with various antigens. Results: All organisms were lab...
Article
Background Determining what quarantine period and detection strategy are more effective and sustainable remains a challenge for further prevention and social stability. Methods From October 2020 to December 2021, 290,547 inbound overseas travelers were subject to government quarantine in Xiamen, China. The detection rate of COVID-19 during differe...
Article
Context.— Neutralizing antibody detection can assess the incidence of coronavirus disease 2019 (COVID-19) and the effectiveness of vaccines. However, commercial reagents for neutralizing antibodies were developed after the anti- severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (Ig) G and IgM antibodies. Therefore, some la...
Article
Exosomes were enriched from plasma by ultracentrifugation, precipitation, and membrane-based approaches for yield and purity. Using the four isolation approaches, particles with mode sizes within the expected range (50–200 nm) can be isolated. By protein estimation, polymer precipitation resulted in a maximum yield (5610.59 ± 51.189 µg/mL), followe...
Article
Full-text available
Background Long noncoding RNAs (lncRNAs) are closely associated with the development of hepatocellular carcinoma (HCC). The present study conducted a genome-wide microarray analysis and qPCR validation to obtain comprehensive insights into this issue. Methods Thirty male HCC patients with chronic HBV infection were included in the present study. P...
Article
Full-text available
Tenofovir and entecavir are currently designated as the preferred oral antiviral drugs for chronic hepatitis B. However, only less than 40% of patients can achieve HBeAg seroconversion. We aim at investigating the role of intestinal microbiome in HBeAg seroconversion induced by oral antiviral therapy and describe multi-omics characteristics of HBeA...
Article
Full-text available
In total, 49 clinical samples were analyzed using two typing schemes, Enhanced Centers for Disease Control and Prevention (ECDC) and multilocus sequence typing (MLST), to describe the molecular characteristics of circulating Treponema pallidum isolates in Xiamen between 2016 and 2017. In addition, genetic mutations potentially related to antibiotic...
Article
Objective: Long noncoding RNAs (lncRNAs) are widely involved in the carcinogenesis and development of cancers. We conducted a meta-analysis to evaluate the diagnostic performance of lncRNAs in hepatocellular carcinoma (HCC). Methods: After the inclusion and exclusion process, relevant information was extracted. Heterogeneity between studies was...
Preprint
Background The long non-coding RNAs (lncRNA) have been closely associated with the development of hepatocellular carcinoma (HCC). The present study conducted a genome-wide microarray analysis and qPCR validation to gain a comprehensive insight on this issue. Methods Thirty male HCC patients with chronic HBV infection were included in the present st...
Article
Introduction: CXCL14 was a significantly under-expressed mRNA in hepatocellular carcinoma tissues according to our microarray analysis, as well as head and neck squamous cell carcinoma and cervical squamous cell carcinoma. CXCL14 was considered a tumor suppressor in some studies; however, its role in HBV infection has not been identified. Methods...
Article
Introduction: Recently, neurosyphilis was found to be associated with diabetes mellitus (DM). Whether the association was specific to neurosyphilis among central nervous system (CNS) infections, and whether neurosyphilis is associated with other prevalent metabolic disorders deserves further study. Methods: An in-depth cross-sectional study was...
Article
Full-text available
Background: This study aimed to evaluate real-time polymerase chain reaction coupled with multiplex probe melting curve analysis (PCR-MCA) for pathogen detection in patients with suspected bloodstream infections (BSIs). Methods: A PCR-MCA assay was developed for simultaneous identification of 28 kinds of the most common pathogens and two resistance...
Article
Vascular inflammation is a complex and multifactorial pathophysiological process that plays a crucial role in all stages of syphilis and is responsible for tissue damage. Little is known about the interactions of infiltrating immunocytes with human dermal vascular smooth muscle cells (HDVSMCs) in arterioles during the immunopathogenesis of syphilis...
Article
Full-text available
The mechanism underlying the stealth property of neurosyphilis is still unclear. Global metabolomics analysis can provide substantial information on energy metabolism, physiology and possible diagnostic biomarkers and intervention strategies for pathogens. To gain better understanding of the metabolic mechanism of neurosyphilis, we conducted an unt...
Article
Background Global metabolomics analysis can provide substantial information on energy metabolism, physiology, possible diagnostic biomarkers and intervention strategies for pathogens. Objective To gain a better understanding of the mechanisms of syphilis and analysis of serum metabolite profiles in syphilis patients. Methods we conducted an untar...
Article
Full-text available
Background The highly variable tprK gene of Treponema pallidum has been acknowledged to be one of the mechanisms that causes persistent infection. Previous studies have mainly focused on the heterogeneity in tprK in propagated strains using a clone-based Sanger approach. Few studies have investigated tprK directly from clinical samples using deep s...
Data
Comparison of the results of NGS and clone-based Sanger sequencing in V6 of the X-8 strain. RF values indicate the relative frequency of each sequence. (TIF)
Data
The primers for tprK amplification. (DOCX)
Data
A sum of the lengths of distinct nucleotide sequences within each V region of tprK from each sample. (XLSX)
Data
The nucleotide sequences within the seven variable regions (V1-V7) of tprK captured directly from 14 primary syphilis clinical samples. (XLSX)
Data
The amino acid sequences within the seven variable regions (V1-V7) of tprK captured directly from 14 primary syphilis clinical samples. * indicates synonymous nucleotide sequences within the same strain. (XLSX)
Preprint
Full-text available
Background The highly variable tprK gene of Treponema pallidum has been acknowledged to be the cause of persistent infection. Previous studies mainly focused on the heterogeneity in tprK in propagated strains using a clone-based Sanger approach. Few studies have investigated tprK directly from clinical samples using deep sequencing. Methods/Princip...
Article
Full-text available
Background The involvement of inflammasome activation and macrophage polarization during the process of syphilis infection remains unknown. In this study, A series of experiments were performed using human macrophages to research the role of NLRP3 inflammasome regulation in interleukin (IL)-1β production and its influence on macrophage polarization...
Article
Anticardiolipin antibody (ACA) includes beta2-glycoprotein I-dependent (β2-GPI-dependent) and β2-GPI-independent forms. The appearance of β2-GPI-dependent ACA and its association with blood coagulation have never been investigated in subjects with classical biological false-positive syphilis reactions (CBFP). In total, 146 CBFP subjects, 465 syphil...
Article
In this work, we employed real-time PCR analysis targeting tp0574 to investigate the effects of different processing procedures on the yield of T. pallidum DNA from blood to improve assay sensitivity. The T. pallidum DNA yields following red blood cell lysis pretreatment were 40.4 times greater from whole blood and 32.4 times greater from residual...
Article
The origin of nontreponemal antibodies during syphilis infection is hotly debated. Here, we analysed the immune response in rabbits immunized with various antigens. Inactivated treponemes elicited the production of low-titre nontreponemal antibodies in some rabbits. Cardiolipin combined with bovine serum albumin also induced anti-cardiolipin antibo...
Article
The polarization of macrophages and the molecular mechanism involved during the early process of syphilis infection remain unknown. This study was conducted to explore the influence of Treponema pallidum (T. pallidum) treatment on macrophage polarization and the Akt-mTOR-NFκB signaling pathway mechanism involved in this process. M0 macrophages deri...
Article
Full-text available
Background: Because of the high prevalence and absence of cure for infection, chronic hepatitis B virus (HBV) infection has been acknowledged as a pressing public health issue. Toll-like receptors (TLRs) activate the human innate immune system and the polymorphisms in TLRs may alter their function. The present study aimed to investigate the associ...
Article
Full-text available
This study aimed to determine whether a serological response could predict the normalization of cerebrospinal fluid (CSF) abnormalities at 6 months after treatment in human immunodeficiency virus (HIV)-negative neurosyphilis patients. A total of 123 neurosyphilis patients were recruited at baseline, 58 of these patients undergoing treatment, repeat...
Article
Full-text available
Treponema pallidum ssp. pallidum (T. pallidum), the causative agent of the sexually transmitted disease syphilis, is an uncultivatable human pathogen. The geographical differences in T. pallidum genomes leading to differences in pathogenicity are not yet understood. Presently, twelve T. pallidum genomes are available to the public, all of which are...
Data
Functional category of CDS in the T. pallidum Amoy strain. (TIF)
Data
Gap information for the Amoy strain in comparison with the reference strain SS14. (XLSX)
Data
Gene ontology enrichment analysis of genes in the Amoy strain. (TIF)
Article
Objectives: The aim of this study was to evaluate the risk factors for pneumonia due to extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL-KP) and to analyse the molecular epidemiology of ESBL-KP in Xiamen, China. Methods: A case-control study was conducted at Zhongshan Hospital from January 2014 to August 2015. Medical records...
Article
Full-text available
Known predictors of neurosyphilis were mainly drawn from human immunodeficiency virus (HIV)-infected syphilis patients, which may not be applicable to HIV-negative populations as they have different characteristics, particularly those with neurological symptoms. This study aimed to identify novel predictors of HIV-negative symptomatic neurosyphilis...
Article
Full-text available
Background Known predictors of neurosyphilis were mainly drawn from human immunodeficiency virus (HIV)-infected syphilis patients, which may not be applicable to HIV-negative populations as they have different characteristics, particularly those with neurological symptoms. This study aimed to identify novel predictors of HIV-negative symptomatic ne...
Article
Background: The rabbit infectivity test (RIT) was previously described as a highly-sensitive method for clinically detecting Treponema pallidum. But our primary study indicated this result may have changed in current antibiotics era. Methods: By inoculating rabbits testis with cerebrospinal fluid (CSF) (n=63) and exudate from hard chancre lesion...
Article
Background: Neurosyphilis (NS) is difficult to diagnose, especially in syphilis patients with negative cerebrospinal fluid (CSF) rapid plasma reagin (RPR) or Venereal Disease Research Laboratory (VDRL) tests. Methods: We conducted a cross-sectional study and an analysis of macrophage migration inhibitory factor (MIF) in syphilitic patients to id...
Article
The contribution of reactive oxygen species (ROS) to antimicrobial lethality was examined by treating Escherichia coli with dimethyl sulfoxide (DMSO), an antioxidant solvent frequently used in antimicrobial studies. DMSO inhibited killing by ampicillin, kanamycin, and two quinolones with little effect on MIC. DMSO-mediated protection correlated wit...
Article
This study aimed to comprehensively evaluate factors that influence the likelihood of syphilis infection from risk-taking behaviours and medical conditions. A retrospective case-control study was conducted by enrolling 664 syphilis inpatients (excluding 11 congenital syphilis patients) and 800 sex- and age-matched controls. Medical histories, clini...
Article
No gold standard currently exists for the diagnosis of general paresis (GP), thus often resulting in unnecessarily delayed therapeutic decision. A retrospective chart review was performed for 85 inpatients with GP in Zhongshan Hospital, Medical College of Xiamen University, and the characteristics of their clinical profiles, serum and cerebrospinal...
Article
We developed a new BOSON chemiluminescence immunoassay (CIA) and evaluated its application with cross-sectional analyses. Our results indicated that BOSON CIA demonstrated strong discriminatory power in diagnosing syphilis, and it can be used as a first-line screening test for syphilis serodiagnosis using the European Centre for Disease Prevention...
Article
Full-text available
Objectives We investigated a diarrhea outbreak that occurred at a university in China to identify the etiological agent of the outbreak, source of infection, mode of transmission, and risk factors. Methods In this case–control study, we compared the food sources and examined the food and water items consumed between the probable and confirmed case...
Article
Objectives: To learn the prevalence of the primary classical broad-host-range (BHR) IncA/C, IncN, IncP, IncQ, and IncW plasmids in dominant gram-negative bacilli from inpatients in a teaching hospital in southern China. Methods: A multiplex polymerase chain reaction based on the replicons of BHR IncA/C, IncN, IncP, IncQ, and IncW plasmids was de...
Article
To identify the etiology of an aseptic encephalitis outbreak (ten cases) in a hospital of Xiamen city from 11 to 17 May, 2011. A total of ten patients' throat swabs, anal swabs and cerebrospinal fluid were collected and detected by RT-PCR for pan-enterovirus. The samples containing detectable pan-enterovirus were tested by PCR with genotype-specifi...
Article
To evaluate the clinical performance of a probe melting analysis (PMA)-based real-time PCR detection kit in rapid detection of rifampin-resistant mutations in Mycobacterium tuberculosis (MTB). The specificity of the assay was evaluated by detecting 37 non-tuberculous mycobacteria (NTM), and the detection limit of the method was evaluated by genomic...
Article
Full-text available
A total of 289 Rhipicephalus (Boophilus) microplus Canestrini (Acari: Ixodidae) collected from cattle in Xiamen city of Fujian province, southeastern China, were tested for ehrlichial agents by nested polymerase chain reaction assay. Twenty-seven (9.3%) ticks from seven cattle were found positive for Ehrlichia. The polymerase chain reaction product...
Article
OBJECTIVE: To evaluated the independent effects of different types of smoking exposure along with multiple risk factors for hepatocellular carcinoma (HCC) and determined whether the magnitude of smoking was modified by other risk factors, both in men and women. METHODS: We conducted a case-control study in Xiamen China. 345 HCC patients and 961 hea...
Article
To explore the relationship between polycyclic aromatic hydrocarbon (PAH) exposure and hepatocellular carcinoma (HCC), and the interaction of PAH exposure and other HCC risk factors to HCC. Baseline blood samples, collected from 345 HCC cases and 961 controls, were used to determine the level of PAH-albumin adducts by competitive enzyme-linked immu...
Article
To investigate the detection limit of multicolor combinational probe coding real-time PCR (MCPC-PCR) in detection of Salmonella and Staphylococcus aureus suspended in the food samples, and to apply MCPC-PCR to detect the samples of food poisoning. Series concentration of bacterium suspension (10(1) - 10(9) CFU/ml) was prepared by using 22 simulated...

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