Jian G Qin

• PhD
• Professor at Flinders University

The understanding of how nitrogen (N) to phosphorus (P) ratios regulate growth and chemical composition of algae is important to control the nutritional value of microalgae for industrial application. This study compared the impacts of N:P ratio manipulations on the growth, elements, lipid, fatty acids and protein contents of Tisochrysis lutea and...

This review examines the initial swimbladder inflation in cultured finfish larvae and the mechanisms controlling body buoyancy and the distribution of larvae during the critical early development stages. The literature suggests that the body buoyancy of larvae affects their distribution in the tank and fish with low buoyancy are likely to sink to t...

The development of digestive enzymes was examined in laboratory-reared yellowtail kingfish larvae from hatching to 36 days after hatching (DAH). The specific activities of amylase, lipase, and alkaline phosphatase showed three distinct phases: a sharp increase in enzyme activity from hatching to the onset of exogenous feeding on 3 DAH, followed by...

The growth and lipid content of three Botryococcus braunii strains from China (CHN), United Kingdom (UK) and Japan (JAP) were compared at three temperatures (20, 25 and 30 ∘C), three irradiances (60, 100 and 300 W m−2) and four salinities (0, 0.15, 0.25, and 0.5 M NaCl) for 30 days, respectively. In the temperature trial, the UK strain and JAP stra...

Compensatory growth of juvenile barramundi, Lates calcarifer (Bloch) was investigated at 28 degreesC for 8 weeks. Fish were divided into four feeding groups including one group with continuous feeding (C) and three other groups with food deprivation for 1 week (SI), 2 weeks (S2) and 3 weeks (S3), respectively. All starved fish resumed feeding in we...

Digestive enzyme activities are known to fluctuate in abalone grown under different environmental conditions however the effects of different dietary regimes are less clear. Therefore, in a 12‐week study, the effects of water temperature and dietary protein levels on digestive enzyme activity in postweaned (6‐month‐old) greenlip abalone (Haliotis l...

Pacific oysters (Crassostrea gigas) and Mediterranean mussels (Mytilus galloprovincialis) are commercially important marine bivalves that frequently coexist and have overlapping feeding ecologies. Like other invertebrates, their gut microbiota is thought to play an important role in supporting their health and nutrition. Yet, little is known regard...

Myo-inositol serves as an essential nutrient in many animal species. However, the optimal requirements of myoinositol and the mechanisms by which myo-inositol is involved in nutritional regulation in crustaceans are unclear. An 8-week trial was conducted to comparatively evaluate the effects of 6 levels of dietary myo-inositol (20, 528, 1030, 2060,...

Selenium (Se) is an essential trace element associated with animal growth and antioxidant and metabolic processes. However, whether Se, especially organic Se with higher bioavailability, can alleviate the adverse effects of low salinity stress on marine economic crustacean species has not been investigated. Accordingly, juvenile Pacific white shrim...

This study investigates the effects of replacing soybean meal with fermented cottonseed meal (FCSM) in low fishmeal diets on the growth performance, antioxidant capacity, immune capacity, digestive enzyme activities and intestinal microbiota of Litopenaeus vannamei. Six isonitrogenous and isolipidic diets were prepared, including the conventional f...

The nutritional value and meat quality influence the consumer preference for crab. Dietary nutrition manipulation is a practical way to regulate flesh quality. This study evaluates the effects of dietary myo-inositol on the nutritional value and meat flavor of male Chinese mitten crab (Eriocheir sinensis). Three diets supplemented with different my...

Pacific oysters ( Crassostrea gigas ) and Mediterranean mussels ( Mytilus galloprovincialis ) are commercially important marine bivalves that frequently coexist and have overlapping feeding ecologies. Like other invertebrates, their gut microbiota is thought to play an important role in supporting their health and nutrition. Yet, little is known re...

Phospholipids have an important antioxidant effect on animals. The effects of different dietary phospholipid sources on the growth, antioxidant activity, immunity, and gut microbiota of female broodstock of Pacific white shrimp Litopenaeus vannamei were investigated. Four isoproteic and isolipid semi-purified diets containing 4% soybean lecithin (S...

Molting activity and innate immunity play a pivotal role in the development and growth of crustaceans and are related to mortality and body abnormality. To evaluate the synergistic effect of vitamin D3 (vit-D3) and Ca in molting and immunity in Chinese mitten crab (Eriocheir sinensis), the crabs (3.20 ± 0.01 g) were fed six diets formulated with tw...

Vegetable oils (VOs) are widely used in the aquatic feed industry because of their stable source and low price, but they can cause a series of adverse effects on aquatic animals. Myo-inositol (MI) plays a vital role in regulating lipid metabolism and the health of aquatic animals. However, it remains unclear whether MI has a regulatory role on the...

The environmental accumulation of thiamethoxam has increasingly become a risk for the health of aquatic animals, especially crustacean species in the same phylum as the target pests. The lack of knowledge on the toxicity of thiamethoxam to crustaceans motivates our research to study the acute and chronic toxicity of decapod crustaceans Litopenaeus...

Research on nutrition and feed development for the broodstock of the Pacific white shrimp, Litopenaeus vannamei, is rare, and a poor broodstock quality is a critical factor restricting the seed supply in shrimp farming. As an essential nutrient for the gonadal development of L. vannamei, one control diet (no phospholipid) and three typical phosphol...

This study investigated the effect of different dietary selenium (Se) sources on the growth performance, antioxidant capacity, gut microbiota, and molecular responses of the Pacific white shrimp Litopenaeus vannamei. Four Se sources (sodium selenite, L-selenomethionine, selenium yeast, or Se nanoparticles) were added to purified diets to 0.4 mg Se/...

Acidized water environment can impact many physiological processes of aquatic animals. The response of the head kidney to acidification, especially the immune response, is of great significance to health. This study analyzed the histological and transcriptional changes under different acidification levels (C group, pH 8.1; P group, pH 7.4; E group,...

The widespread use of neonicotinoid insecticides, such as imidacloprid, in agriculture is one of the key factors for the drop in the survival of invertebrates, including crustacean decapods. However, there is currently a lack of comprehensive studies on the chronic toxicity mechanisms in crustacean decapods. Here, the concentration-dependent effect...

Copper (Cu) is a widespread pollutant in water and has posed a significant threat to aquatic animals' health. It is essential to find a strategy to reduce the adverse effects of copper on aquatic organisms. This study investigated the role of myo-inositol (MI) in the reducing chronic toxicity induced by copper in Chinese mitten crab (Eriocheir sine...

The increasing demand for fish produced by aquaculture industries has led to a high demand of live feeds, such as larvae. Further development of culture techniques is important to lower the production costs as well as to maximize the production of natural food for larval rearing. Moina macrocopa is a potential freshwater live feed that can replace...

To elucidate the possible molecular reaction of vertebral fusion in juvenile golden pompano at high temperature, we examined the changes in growth, vertebral fusion incidence, histological differences and transcription levels of genes related to bone development in juvenile golden pompano at 27 and 33°C for 30 days, respectively. The growth rate of...

This study evaluated the effects of dietary myo -inositol (MI) on growth performance, body composition, antioxidant status and lipid metabolism of juvenile Chinese mitten crab ( Eriocheir sinensis ) fed different percentage of lipid. Crabs (4.58 ± 0.05 g) were fed four diets including a normal lipid diet (N, containing 7% lipid and 0 mg/kg MI), N w...

This study investigates the effect of arginine (Arg) on the growth, antioxidant capacity, inflammation and immunity of juvenile Chinese mitten crab Eriocheir sinensis under high pH stress. Three diets containing 23.7 g/kg (control) 31.4 g/kg and 40.1 g/kg Arg were formulated and fed to crabs (3.37 ± 0.01 g) at high pH (pH = 9.5) for eight weeks. Th...

Lipid molecules and fatty acids were analyzed using lipidomics to understand the change of lipid structure and metabolism in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis). The crabs fed diets with four oil sources (palm oil, olive oil, safflower seed oil and purple perilla oil) for 8 weeks. Palmitic acid, oleic acid, linoleic acid,...

This study investigates the effects of vitamin D3 (VD3) on growth performance, antioxidant capacity, immunity and molting of larval Chinese mitten crab Eriocheir sinensis. A total of 6,000 larvae (7.52 ± 0.10 mg) were fed with six isonitrogenous and isolipidic experimental diets with different levels of dietary VD3 (0, 3000, 6000, 9000, 12000 and 3...

This study characterizes the composition, diversity, function and interspecies interaction of the bacteria community in the gut of shrimp (Penaeus japonicus) and its culture water and sediment by 16S rRNA gene high-throughput sequencing. The relationships of bacterial communities in the gut and the surrounding water and pond sediment were compared...

This study was to evaluate the relationship between myo-inositol synthesis and carbohydrate metabolism in Mozambique tilapia (Oreochromis mossambicus). Fish were immediately transferred from 0 psu (practical salinity units, the control) to 20 psu for 96 h with 60 fish in each group. Compared with the control group, the volume of the mitochondria-ri...

Understanding the energy budgets of cultured abalone fed graded dietary crude protein levels at different water temperatures gives us the ability to optimise diets for different seasons. In this 91-d study we investigated the effects of feeding four diets containing graded levels of crude protein (CP; 27, 30, 33 and 36%) at three seasonal water tem...

The present research aimed to investigate the protection of N-acetylcysteine (NAC) on T-2 toxin-induced toxicity in Chinese mitten crab (Eriocheir sinensis). Crabs (0.27 ± 0.00 g) were fed four different diets (Control, T-2, 0.05% NAC + T-2 and 0.1% NAC + T-2) for 8 weeks. The results showed that T-2 toxin decreased growth performance, and induced...

This study investigated the effects of different levels of dietary selenium (Se) on the growth, antioxidant capacity, hepatopancreas health, and transcriptional-level responses of Litopenaeus vannamei. Sodium selenite was added to basal diet formulations at 0, 0.2, 0.4, and 0.8 mg/kg, with final Se concentrations of 0.13, 0.20, 0.45, and 0.81 mg/kg...

This study investigated the impact of water temperature on survival, skeletal development and the molecular mechanism of temperature-induced spinal deformity in golden pompano Trachinotus ovatus. Fish larvae were subjected to three temperatures (26, 29 and 32 • C) from 0 to 32 days post-hatch (DPH). On 32 DPH, significant higher growth rate was obs...

Myo-inositol (MI) as a major intracellular osmolyte can be accumulated to protect cells from a variety of stress. The present study was to evaluate the effects of dietary MI on growth, osmotic response and transcriptional response in the liver of euryhaline fish Oreochromis mossambicus under long-term salinity stress. Five isonitrogenous and isolip...

Simple Summary Long-term hypersaline stress can induce coagulation disorders and splenomegaly and down-regulate the complement pathway in tilapia, which can increase risk in healthy breeding. As a prebiotic, β-glucan dietary supplementation can significantly reduce enlarged spleen resulting from hypersaline stress. The hematological aspects of the...

Simple Summary Choline is a vitamin-like nutrient and has many metabolic and physiological functions in aquatic animals. Unfortunately, the information on the optimal requirement of dietary choline in Litopenaeus vannamei is limited, and the molecular and metabolic mechanisms of choline on L. vannamei are unclear. Hence, in this study, the growth p...

To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The...

In Australia, commercially farmed greenlip abalone (Haliotis laevigata Donovan), fed formulated diets display a faint lip and dark foot colour in comparison with wild abalone. This study evaluates the impact of macroalgal diets and water temperatures on the colour and tissue pigment deposition in farmed greenlip abalone. Three diets are (a) fresh U...

Energy budgets were developed for greenlip abalone (Haliotis laevigata) under different feeding scenarios to predict growth, metabolic and waste outputs. The energy budgets of abalone fed live macroalgae (Ulva sp. or Gracilaria cliftonii) and an equal mix of those two macroalgae species with and without nutrient enrichment, or one of three control...

Macroalgae meal may provide a useful source of dietary energy for cultured abalone. The inclusion of dried macroalgal meals in formulated diets may also provide other benefits such as improvement in growth, health and survival. In this 92 d study, energy allocation in Greenlip Abalone, Haliotis laevigata, fed either a commercial diet, the control d...

The current study aims to investigate the effects of dietary T-2 toxin on the intestinal health and microflora in the juvenile Chinese mitten crab (Eriocheir sinensis) with an initial weight 2.00 ± 0.05 g. Juvenile crabs were fed with experimental diets supplemented with T-2 toxin at 0 (control), 0.6 (T1 group), 2.5 (T2 group) and 5.0 (T3 group) mg...

This study elucidates the effects of chronic pH stress on the growth and metabolic response of juvenile Chinese mitten crab Eriocheir sinensis. Crabs were exposed under normal pH (control, pH = 8.0 ± 0.20), low pH (pH = 6.5 ± 0.20), and high pH (pH = 9.5 ± 0.20) in an 8-week trial. Both low and high pH suppressed weight gain but low pH had more adv...

This study evaluated the effects of dietary myo ‐inositol (MI) levels on growth, body composition, antioxidant status, non‐specific immunity and lipid metabolism in juvenile Chinese mitten crab (Eriocheir sinensis ). A total of 960 crabs was fed with six diets supplemented with graded levels of MI (0, 204, 406, 810, 1,650 and 3,211 mg/kg) for 8 wee...

The follistatin-related protein (FRSP) gene performs numerous functions through binding with cell regulatory proteins in transforming growth factor beta (TGF-β) superfamily such as activin and myostatin. The FRSP gene has been extensively studied in human and other vertebrate models such as chick and mouse to explore its role for disease control an...

Gut microbiota in fish plays an important role in the nutrient digestion, immune responses and disease resistance. To understand the effect of fluoroquinolone antibiotic bath administration on fish gut microbiota, the gut microbiota community in the coral trout Plectropomus leopardus (Lacepède, 1802) was studied after enrofloxacin bathing treatment...

Environmental pH change is a challenge for growth and health of aquatic animals. In this study, the juveniles of Pacific white shrimp Litopenaeus vannamei were exposed to a higher pH 9.5 or a lower pH 6.5 versus a normal pH (pH 8.0) as the control for four weeks. Growth performance, antioxidant capacity, immune functions, and intestinal histologica...

To explore the feasibility of land-based farming on mackerel tuna (Euthynnus affinis), the changes of antioxidant, immune and digestive enzyme activities of wild caught and indoor cultured juvenile mackerel tuna were evaluated. The activities of total superoxide dismutase, catalase, peroxidase and alkaline phosphatase in the liver of the wild group...

The T-2 toxin is a trichothecene mycotoxin and is highly toxic to aquatic animals, but little is known on its toxic effect in crustaceans. In the present study, the crab juveniles were fed with diets containing four levels of T-2 toxin: 0 (control), 0.6 (T1), 2.5 (T2) and 5.0 (T3) mg/kg diet for 56 days to evaluate its impact on the juvenile of Chi...

Suspension-feeding bivalves are the keystone species that affect the abundance and composition of phyto-plankton communities. This study compares the food selectivity of oysters Crassostrea gigas (Thunberg, 1973), mussels Mytilus galloprovincialis (Lamarck, 1819), and cockles Katelysia rhytiphora (Lamy, 197) on natural particle assemblages in the l...

This study evaluates the effects of different dietary sources of non-protein energy on growth performance, histological structure, antioxidant status and immune response of barramundi Lates calcarifer. Fish were fed with isoenergetic diets (18 kJ/g) with two types of non-protein energy in the experimental groups and a regular diet was used as the c...

This study evaluates whether a higher level of dietary protein could alleviate pH stress in Chinese mitten crab. Three diets with protein levels at 30%, 35% and 40% were used to feed crab for 8 weeks to determine the differential effect of dietary protein on growth, antioxidant capacity, inflammatory response and apoptotic factors at normal pH (7.8...

Recruiting King George whiting Sillaginodes punctatus were studied to assess the potential for food competition with permanent resident fish species in a nursery habitat. Marine migrant post larval S. punctatus (<60 mm TL) consumed primarily harpacticoid copepods and had high (>0.6) diet overlap with permanent resident fish species Favonigobius let...

β-conglycinin is a major component of feed allergens that can cause inflammation and dysfunction of intestinal digestion and absorption in fish, but little is known in crab. This study investigated the mitigative effects of N-acetylcysteine (NAC) on growth inhibition and intestinal damage induced by β-conglycinin in juvenile Chinese mitten crabs (E...

This study evaluates the effect of dietary supplementation of immunostimulants on the Chinese mitten crab (Eriocheir sinensis) with a single administration of mannan oligosaccharide (MOS), or its combination with either β-glucan or with inulin for 8 weeks. Four diets included an untreated control diet (C), MOS alone (3 g kg-1, M), MOS with β-glucan...

To investigate the effects of arginine (Arg) on the growth, antioxidant capacity, immunity and disease resistance of juvenile Chinese mitten crab, three diets containing Arg levels at 1.72% (control), 2.73% and 3.72% were formulated and fed to Chinese mitten crab (0.22 ± 0.03 g) for eight weeks. The weight gain, ecdysterone and growth hormone in th...

The effects of system partition on the water quality were studied using three shrimp ponds (Ts), and each one of the shrimp pond was connected individually with a fish pond by piping and pump. Three other isolated shrimp ponds were designated as the Cs treatment. The results showed that the implementation of the system can improve the water quality...

The increase of dietary lipid utilization leads a supply of more energy to reduce protein breakdown and promote protein deposition in fish. Therefore, a search for feed additives to stimulate lipid metabolism interests fish nutritionists. Diacylglycerol oil (DGO), as a safe food additive in human, can increase lipid metabolism and prevent obesity i...

Mannan oligosaccharide (MOS) is a common prebiotics to improve health and immunity of animals in aquaculture. However, its optimum level and effects on the immune response in crab are currently unknown. The present study investigated the optimum level of dietary MOS supplementation and its effects on growth performance, antioxidant capacity, non-sp...

A study on Chlamys nobilis sperm cryopreservation by a programmable freezing method was conducted under laboratory condition. Four cryoprotectant agents (dimethyl sulfoxide [DMSO], methanol [MET], propanediol[PG] and ethylene glycol [EG]) and four concentrations (5%, 10%, 20% and 30%) were evaluated for their ability to retain sperm motility, movem...

Understanding fish larval development is of a great interest for aquaculture production efficiency. Identifying possible indicators of fish larvae stress could improve the production and limit the mortality rate that larval stage is subjected to. Heat-shock proteins (HSPs) and heat-shock factors (HSFs) are well known as indicators of response to ma...

The α-lipoic acid (α-LA) is a novel feed additive to improve growth, antioxidant capacity and lipid metabolism in animal husbandry. In this study, isonitrogenous and isoenergetic diets were formulated at seven levels of α-LA (0, 300, 600, 1200, 2400, 4800, or 9600 mg/kg) and fed to the Chinese mitten crab Eriocheir sinensis juveniles for eight week...

_The acute toxicity of nitrite (133.33, 266.66, 399.99mg/L) to 15 days posthatched Lates calcarifer (barramundi) was studied by semi-static bioassay method; 0 mg/L nitrite was used as control. Samples were obtained at 0, 6, 12, 24, 36, 48, 72 and 96 h respectively to measure and analyze the activity of antioxidant, digestive enzymes, and concentrat...

To understand the impacts of peanut worms Sipunculus nudus on the prokaryotic community composition in a tidal flat, an onsite investigation was conducted in Suixi in the Beibu Gulf (109.82E, 21.35N) in the burrow sediments, non‐burrow sediments and the sediments without peanut worm disturbance (control). The16S rRNA gene Illumina MiSeq sequencing...

The wide use of lipid as a non‐protein energy substitute has led to lipid metabolic problems in cultured tilapia. Therefore, studies that reduce the effects of high‐fat diets in genetically improved farmed tilapia (GIFT) are required. This study evaluated the optimum level and effects of dietary α‐lipoic acid (α‐LA) on growth performance, body comp...

A 74‐day experiment was conducted to evaluate the production performance and water quality variation in three types of farming system for largemouth bass Micropterus salmoides. The tested aquaculture models included monoculture of largemouth bass (MC), polyculture of largemouth bass, gibel carp Carassius auratus gibelio and silver carp Hypophthalmi...

Summer mortality of some bivalve species is often associated with the change of environmental temperature. This study compares the response of immunological parameters to temperature change in three marine bivalves: Pacific oyster Crassostrea gigas, Mediterranean mussel Mytilus galloprovincialis and mud cockle Katelysia rhytiphora. Each species was...

High fat diets are commonly used in aquaculture to reduce feed cost in Nile tilapia, but impair its lipid homeostasis. This study evaluated the role of forskolin on reducing fat accumulation in Nile tilapia (Oreochromis niloticus) by using in vitro and in vivo experiments. The use of 50 μM forskolin in vitro increased free fatty acid and glycerol r...

This study elucidates the response to nitrite stress and the effect of dietary selenium supplements on the growth, antioxidant activity, immunity and transcriptome of juvenile Chinese mitten crab Eriocheir sinensis. In the control group, the crabs were fed the diet without selenium supplementation and there was no nitrite addition to the water. In...

This study compared the efficiency of different diets on the growth, survival and reproduction of the cyclopoid copepod Apocyclops ramkhamhaengi as a potential live food species for fish larvae and crustaceans in aquaculture. The experimental diets consisted of four mono diets (chicken manure, palm kernel cake (PKC), Tetraselmis sp. and Nannochloro...

This study explored the impact of fishmeal replacement by commercial soybean meal (SM) and EnzoMeal (EZM) on Asian seabass Lates calcarifer growth performance using six diets. The six diets comprised two sources of plant proteins with three levels each, including 300 g kg⁻¹ soybean meal (SM30), 300 g kg⁻¹ EnzoMeal (EZM30), 400 g kg⁻¹ soybean meal (...

Five isonitrogenous diets were formulated with graded alpha‐linolenic acid (LNA) levels (0, 5, 10, 15 and 20 g/kg) to investigate LNA requirement of juvenile Russian sturgeon Acipenser gueldenstaedtii. Weight gain and specific growth rate of fish fed LNA5 and LNA10 were significantly higher than those in other groups, while the feed conversion rati...

This study compared the efficacy of different dietary algae on the growth andreproduction of the cyclopoid copepod Cyclopina kasignete, a potential live food species forfish larvae in aquaculture. The experimental diets for the copepod consisted of three monoalgaldiets (Nannochloropsis oculata, Tisochrysis lutea and dry Melosira sp.) and two mixed...