Jesús Mateos Martín

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Verified

Jesús verified their affiliation via an institutional email.

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• PhD
• Senior Researcher at University Hospital Lucus Augusti

Hutchinson-Gilford progeria syndrome (HGPS) is a very rare fatal disease characterized for accelerated aging. Although the causal agent, a point mutation in LMNA gene, was identified more than a decade ago, the molecular mechanisms underlying HGPS are still not fully understood and, currently, there is no cure for the patients, which die at a mean...

Nuclear accumulation of a mutant form of the nuclear protein Lamin-A, called Progerin (PG) or Lamin AΔ50, occurs in Hutchinson-Gilford Progeria Syndrome (HGPS) or Progeria, an accelerated aging disease. One of the main symptoms of this genetic disorder is a loss of sub-cutaneous fat due to a dramatic lipodystrophy. We stably induced the expression...

In this study we tested a semi-automated protocol for the proper storage and conservation in a hospital biobank of tryptic peptide extracts coming from samples with low and high protein complexity for subsequent mass spectrometry analysis. Low-complexity samples (Serum albumin, Serotransferrin and Alpha-S1-casein) were loaded in replicates in SDS-P...

Cysteamine, an aminothiol, is the only available treatment for cystinosis, an incurable metabolic recessive disease characterized by detrimental symptoms at the renal, ocular, and muscular levels. Cystinosis is due to mutations in the CTNS gene encoding for the lysosomal symporter cystinosine. Cysteamine treatment only delays the symptoms, presents...

Background A challenging new branch of research related to aging-associated diseases is the identification of miRNAs capable of modulating the senescence-associated secretory phenotype (SASP) which characterizes senescent cells and contributes to driving inflammation. Methods Mesenchymal stem cells (MSC) from human umbilical cord stroma were stabl...

Introduction Anisakis simplex sensu stricto (s. s.) is one of the most widespread parasitic nematodes of marine organisms, with humans as accidental hosts. While many studies have explored nematode biology and host interactions, the role of extracellular vesicles (EVs) as signaling molecules in parasitic nematodes is less understood. Materials and...

Purpose Adalimumab (ADA) is a systemic biological treatment option approved for the treatment of noninfectious uveitis (NIU); however, up to 40% of patients do not respond to the drug, either in a primary or secondary manner. Here, we evaluated the proteomic profile of patients with NIU who fail to ADA to identify proteins implicated in intraocular...

Citation: Rodríguez-Martínez, L.; Castro-Balado, A.; Hermelo-Vidal, G.; Bandín-Vilar, E.; Varela-Rey, I.; Toja-Camba, F.J.; Rodríguez-Jato, T.; Novo-Veleiro, I.; Varela-García, P.M.; Zarra-Ferro, I.; et al. Ampicillin Stability in a Portable Elastomeric Infusion Pump: A Step Forward in Outpatient Parenteral Antimicrobial Therapy. Pharmaceutics 2023...

Age-related macular degeneration (AMD) is a common ocular disease characterized by degeneration of the central area of the retina in the elderly population. Progression and response to treatment are influenced by genetic and non-genetic factors. Proteomics is a powerful tool to study, at the molecular level, the mechanisms underlying the progressio...

Cystinosis is a rare genetic disorder characterized by the accumulation of cystine crystals in different tissues and organs causing, among other symptoms, severe ocular manifestations. Cysteamine eye drops are prepared in hospital pharmacy departments to facilitate access to treatment, for which vehicles that provide adequate biopermanence, as well...

Context The corpus luteum (CL) is an endocrine gland in the ovary of mature females during the oestrous cycle and pregnancy. There is evidence of a relationship between the secretory function of the CL and PPARs. Aims In this study, we investigated the changes in the proteome of the CL in relation to the phase of the oestrous cycle and the impact...

Anisakis simplex s. s. is a parasitic nematode of marine mammals and causative agent of anisakiasis in humans. The cuticle and intestine of the larvae are the tissues most responsible for direct and indirect contact, respectively, of the parasite with the host. At the L4 larval stage, tissues, such as the cuticle and intestine, are fully developed...

Bacillus sp. HR21-6 is capable of the chemo- and regioselective synthesis of lipophilic partially acetylated phenolic compounds derived from olive polyphenols, which are powerful antioxidants important in the formulation of functional foods. In this work, an acetyl esterase was identified in the secretome of this strain by non-targeted proteomics,...

Helminths are masters at manipulating host's immune response. Especially, parasitic nematodes have evolved strategies that allow them to evade, suppress, or modulate host's immune response to persist and spread in the host's organism. While the immunomodulatory effects of nematodes on their hosts are studied with a great commitment, very little is...

Background. Anisakis simplex is a parasitic nematode of marine organisms. Humans can be accidental hosts for this species. The finding that parasitic nematodes can release extracellular vesicles (EVs) was the breakthrough discovery. The secretion of EVs, as signal molecules, by parasitic nematodes has been poorly studied. This prompted us to charac...

In the present protocol, extracellular vesicles (EVs) released from a primary culture of human umbilical cord mesenchymal stem cells (MSCs) were isolated by ultracentrifugation processes, characterized by transmis�sion electron microscopy (TEM) and measured by nanoparticle tracking analysis (NTA). Protein was extracted from EVs using RIPA buffer an...

This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical...

Anisakis simplex s.s. is a parasitic nematode that causes anisakiasis in humans. L3 stage larvae, which are present in many fish species and cephalopods all over the globe, might be consumed and develop occasionally into the L4 stage but cannot reproduce. Anisakiasis is an emerging health problem and economic concern. In recent years, proteomic met...

Organophosphate flame retardants (OPFRs) are (re-)emergent environmental pollutants increasingly being used because of the restriction of other flame retardants. The chlorinated OPFR, tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is among those of highest environmental concern, but its potential effects in the marine environment have rarely been in...

Ivermectin (IVM), an antiparasitic drug, has a positive effect against Anisakis simplex s.s. infection and has been used for the treatment and prevention of anisakiasis in humans. However, the molecular mechanism of action of IVM on A. simplex s.s. remains unknown. Herein, tandem mass tag (TMT) labeling and extensive liquid chromatography coupled w...

Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10p...

Background In the present study, we explored potential protein biomarkers useful to predict the therapeutic response of knee osteoarthritis (KOA) patients treated with pharmaceutical grade Chondroitin sulfate/Glucosamine hydrochloride (CS+GH; Droglican, Bioiberica), in order to optimize therapeutic outcomes. Methods A shotgun proteomic analysis by...

Anisakis simplex is a parasitic nematode that can cause anisakiosis and/or allergic reactions in humans. The presence of invasive third-stage larvae (L3) in many different consumed fish species and the fourth-stage larvae (L4) in marine mammals, where L3 can accidentally affect to humans and develop as far as stage L4. World Health Organization and...

Parvalbumins beta (β-PRVBs) are the main fish allergens. The only proven and effective treatment for this type of hypersensitivity is to consume a diet free of fish. We present the molecular characterization of B-cell epitopes by shotgun proteomics of different β-PRVBs combined with protein-based bioinformatics and IgE-reactive approaches. The fina...

Our goal was to establish panels of protein biomarkers that are characteristic of patients with microbiologically confirmed pulmonary tuberculosis (TB) and their contacts, including latent TB-infected (LTBI) and uninfected patients. Since the first pathogen-host contact occurs in the oral and nasal passages the saliva and sputum were chosen as the...

RNAseq analysis. RNAseq analysis of Control vs. HGPS cell lines. (XLSX)

Summary of the proteomic analysis. 749 proteins in common were quantificated in both iTRAQ replicates (A). Graphical representation of the Control/HGPS ratios shows a good correlation between the two replicates (B). (TIF)

iTRAQ analysis. iTRAQ analysis (replicates 1 and 2) of Control cells vs. HGPS cell lines. (XLSX)

Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated t...

NC3Rs ARRIVE gidelines checklines checklist-def.pdf. (PDF)

This corrects the article DOI: 10.1038/srep43923.

Stem cells possess significant age-dependent differences in their immune-response profile. These differences were analysed by Next-Generation Sequencing of six age groups from bone marrow mesenchymal stem cells. A total of 9,628 genes presenting differential expression between age groups were grouped into metabolic pathways. We focused our research...

Acinetobacter baumannii is a hospital-acquired pathogen that shows an extraordinary capacity to stay in the hospital environment. Adherence of the bacteria to eukaryotic cells or to abiotic surfaces is the first step for establishing an infection. The A. baumannii strain AbH12O-A2 showed an exceptional ability to adhere to A549 epithelial cells. Th...

Significance: The results of the present work indicate that blood proteins in plasma are broadly insensitive to such preanalytical variables as delayed processing or freeze/thaw cycles when analyzed at the peptide level. Although there are other studies related to protein stability of clinical samples with similar results, what is remarkable about...

Background The high costs incurred when drugs fail during clinical trials has prompted interest in biomarkers as biological indicators for progress of disease, effect of therapeutic interventions, and/or drug-induced toxicity¹. Objectives The aim of this study is to identify predictive protein biomarkers useful to stratify osteoarthritis (OA) pati...

Objective: Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthon's jelly and their directed differentiation toward chondrocyte-like cells capable of regenerating damaged cartilage when transplanted into an injured joint. This study aimed to determine whether lactogenic hormone prolactin (PRL) o...

Mesenchymal stem cells promising role in cell-based therapies and tissue engineering appears to be limited due to a decline of their regenerative potential with increasing donor age. Six age groups from bone marrow mesenchymal stem cells of Wistar rats were studied (newborn, infant, young, pre-pubertal, pubertal and adult). Quantitative proteomic a...

Background: Acinetobacter baumannii is a major health problem. The most common infection caused by A. baumanniiis hospital acquired pneumonia, and the associated mortality rate is approximately 50 %. Neither in vivo nor ex vivo expression profiling has been performed at the proteomic or transcriptomic level for pneumonia caused by A. baumannii. In...

Background Osteoarthritis (OA) is characterized by the progressive loss of cartilage structural extracellular matrix (ECM) components. The release of these proteins from the tissue can vary according to the stage of the disease and the specific joint that is affected. Objectives The aim of this study was to perform a quantitative proteomics approac...

Background The diagnosis methods currently available for Osteoarthritis (OA) are limited and lack sensitivity. There is a considerable interest pointed in the validation of specific OA biomarkers for early diagnosis and disease progression studies in OA. Recently, there has been a remarkable development in mass spectrometry-based methodologies for...

We have previously reported that articular chondrocytes in tissue contain long cytoplasmic arms that physically connect two distant cells. Cell-to-cell communication occurs through connexin channels termed Gap Junction (GJ) channels, which achieve direct cellular communication by allowing the intercellular exchange of ions, small RNAs, nutrients an...

Osteoarthritis (OA) is a degenerative joint pathology characterized by articular cartilage degradation that lacks from efficient therapy. Since previous epidemiological data show a high controversy regarding the role of smoking in OA, we aimed to evaluate the effects of nicotine (the most physiologically active compound of tobacco) on the joint. Se...

Background Osteoarthritis (OA) is the most common rheumatic disease and the major cause of pain and disability along the aging population globally (1, 2, 3). Recently, a panel of experts of the EULAR outlined the priority research needs for the near future, including the search for predictors of OA progression [4, 5]. Objectives The identification...

Osteoarthritis (OA) is the most common rheumatic pathology and is characterized primarily by articular cartilage degradation. Despite its high prevalence, there is no effective therapy to slow disease progression or regenerate the damaged tissue. Therefore, new diagnostic and monitoring tests for OA are urgently needed, which would also promote the...

Osteoarthritis (OA) is the most common age-related rheumatic disease. Chondrocytes play a primary role in mediating cartilage destruction and extracellular matrix (ECM) breakdown, which are main features of the OA joint. Quantitative proteomics technologies are demonstrating a very interesting power for studying the molecular effects of some drugs...

Background Haptoglobin (HPT) is a plasma protein that binds free hemoglobin with high affinity and thereby inhibits its oxidative activity. HPT exists in two allelic forms in the human population, which produces three known phenotypes. These depend on the HPT alpha chains that are expressed (α1, α2 or both), whereas the β chain is always present. I...

Background The low-molecular-weight subset of the cartilage proteome (also termed cartilage peptidome) may have a diagnostic potential in osteoarthritis (OA) research, not yet fully exploited. Objectives The aim of this study is to investigate the cartilage secretome by means of peptidomic analysis and to provide a novel source for OA biomarker di...

Background Osteoarthritis (OA) is characterized by the progressive loss of cartilage structural extracellular matrix (ECM) components, mainly collagenous and non-collagenous proteins. The release of these proteins from the tissue can vary according to the stage of the disease. Characterization of molecular differences between cartilage subtypes wil...

Supplementary Information

Supplementary Table S2

Automated liquid chromatography tandem mass spectrometry (LC-MS/MS) is a well-established technique for identification of components from complex mixtures in shotgun proteomics experiments. Approaches involving the use of matrix-assisted laser desorption/ionization mass spectrometry (LC-MALDI-MS/MS) comprise the preparation of protein extracts, the...

Mesenchymal stem cells (MSCs) are undifferentiated cells with an unlimited capacity for self-renewal and able to differentiate towards specific lineages under appropriate conditions. MSCs are, a priori, a good target for cell therapy and clinical trials as an alternative to embryonic stem cells, avoiding ethical problems and the chance for malignan...

Background Osteoarthritis (OA) is a slowly progressive degenerative disease characterized by degradation of the matrix and cell death, resulting in a gradual loss of articular cartilage integrity. Despite its high prevalence, disease-modifying therapies are not available and this is in part related limited sensitivity of current methods for the ass...

Background Recent works by our group provide evidences for the usefulness of proteomics techniques for pharmacological analyses in the rheumatology field, with the aim to identify efficacy markers for monitoring different OA treatments [1,2]. Objectives To assess the synergistic chondroprotective effect of chondroitin sulfate (CS) and glucosamine h...

Background Mesenchymal stem cells (MSCs) have been identified in bone marrow, as well as in other tissues of the joint. These cells have the ability to differentiate into chondrocytes and also to modulate immune responses, improve angiogenesis and prevent fibrosis. These properties confer them the potential to be used for therapeutic applications i...

Desiccation tolerance contributes to the maintenance of bacterial populations in hospital settings and may partly explain its propensity to cause outbreaks. Identification and relative quantitation of proteins involved in bacterial desiccation tolerance was made using label free quantitation and iTRAQ labelling. Under desiccating conditions, the po...

In the present study, we examined the effect of the over-expression of LMNA, or its mutant form progerin (PG), on the mesoderm differentiation potential of mesenchymal stem cells (MSCs) from human umbilical cord (UC) stroma using a recently described differentiation model employing spheroid formation. Accumulation of lamin A (LMNA) was previously a...

Lignins result from the oxidative polymerization of three hydroxycinnamyl (p-coumaryl, coniferyl and sinapyl) alcohols (monolignols) in a reaction mediated by peroxidases, yielding H, G and S units, respectively. It has been reported that both amount and composition of lignins varies depending on the environmental conditions, and the addition of di...

Background Recent works by our group provide evidences of the usefulness of proteomics techniques for anti-osteoarthritis (OA) drug screening [1-3]. Objectives The aim of this study is to identify protein markers for monitoring chondroitin sulfate (CS) treatment by OA chondrocytes secretome analysis using a quantitative proteomic approach (iTRAQ)....

Introduction Chondroitin sulfate (CS) is a symptomatic slow-acting drug for osteoarthritis (OA) widely used in the clinic. The aim of this work is to find proteins whose secretion from cartilage cells under proinflammatory stimuli (IL-1β) is regulated by CS, employing a novel quantitative proteomic approach. Methods Human articular chondrocytes re...

Human mesenchymal stem cells (hMSCs), residing in bone marrow as well as in the synovial lining of joints, can be triggered to differentiate toward chondrocytes. Thus, hMSCs harbor great therapeutic potential for the repair of cartilage defects in osteoarthritis (OA) and other articular diseases. However, the molecular mechanisms underlying the cho...

The study of the extracellular proteomes of pathogenic bacteria is essential for gaining new insights into the mechanisms of pathogenesis and for the identification of virulence factors. Through the use of different proteomic approaches, namely Nano-LC and 2DE combined with MALDI-TOF/TOF, we have characterized the extracellular proteome of a highly...

Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like cells using a new in vitro model that consists of the random formation of spheroids in a medium supplemented with fetal bovine serum on a nonadherent surface. The medium was changed after 2 days to one specific for the induction of chondrocyte differentiation....