Jesus Gonzalez-Lopez

Jesus Gonzalez-Lopez
University of Granada | UGR · Instituto Universitario del Agua

PhD

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337
Publications
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Publications

Publications (337)
Article
Oleaginous fungi natively accumulate large amounts of triacylglycerides (TAG), widely used as precursors for sustainable biodiesel production. However, little attention has been paid to the diversity and roles of fungal mixed microbial cultures (MMCs) in sequencing batch reactors (SBR). In this study, a lipid-rich stream produced in the fish-cannin...
Article
Next-generation sequencing and metatranscriptomic analysis were used to study the diversity and characterize the gene machinery in a cold bioreactor treating urban wastewater located near the Polar Artic Circle. The bacterial population was dominated by the family Comamonadaceae; archaeal population was highly represented by Methanococcales clones...
Article
Four aerobic granular sludge reactors operated in sequential batch mode were implemented to understand the effect of low (7 °C) and high (26 °C) temperature in the removal of pharmaceuticals in the treated effluent. The chemical oxygen demand and nitrogen removal ratio were affected during the startup period in reactors feeding with drugs, but syst...
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Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. T...
Article
An aerobic granular biofilm process was operated in a sequential batch reactor for treating nitrate-pollute groundwater at 6 ºC, which was fed with a progressively reduced carbon concentration to understand the nitrate removal efficacy in cold regions by denitrifying microorganisms. The evaluation of the performance reflected a quick granulation pr...
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The presence of emerging contaminants in the environment, such as pharmaceuticals, is a growing global concern. The excessive use of medication globally, together with the recalcitrance of pharmaceuticals in traditional wastewater treatment systems, has caused these compounds to present a severe environmental problem. In recent years, the increase...
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The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address...
Article
The detection of the presence of bacteria in water is essential to determine their use and prevent environmental and health problems. Conventional techniques for the detection of certain bacteria, such as Escherichia coli, are very lengthy, require trained operators and can be subjective, since they are based on the capacity of colour discernment o...
Article
The influence of the inoculum on bacterial communities of Spanish-style green table olive brinesis very limited. This work assessed the size and structure of the bacterial communities in olive brines inoculated with Lactiplantibacillus plantarum. Also, a new qPCR assay was developed to determine the specific abundance of this genus. Absolute abunda...
Article
Two endophytic strains, coded MOVP5 T and MOPV6, were isolated from nodules of Phaseolus vulgaris plants grown on agricultural soil in Southeastern Spain, and were characterized through a polyphasic taxonomy approach. Their 16S rRNA gene sequences showed 99.3 and 99.4 %, 98.9 and 99.6 %, and 99.0 and 98.7% similarity to ‘ A. deltaense ’ YIC 4121 T...
Article
Studies that characterize the microbial communities in wastewater treatment plants (WWTPs) are numerous, yet similar studies in industrial WWTPs treating fish-canning effluents are limited. The microbial communities in samples of 4 fish-canning WWTPs that operated under different NaCl concentrations were investigated by qPCR and partial 16S rRNA ge...
Article
Paleosoil accretions of carbonates in the Tandilia system (Southern Pampas, Argentina) dated to the lower/middle Pleistocene age are locally referred to as tosca. The characterization of this indurated layer of carbonates were analyzed via a biophysicochemical approach, including, physicochemical analysis of soils, mineral x-ray diffraction of the...
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A sequential bed granular bioreactor was adapted to treat nitrate-polluted synthetic groundwater under anaerobic conditions and agitation with denitrification gas, achieving very efficient performance in total nitrogen removal at influent organic carbon concentrations of 1 g L-1 (80–90%) and 0.5 g L-1 (70–80%) sodium acetate, but concentrations bel...
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Biodeterioration is one of the most important processes in metal pipeline corrosion, and it can be due to physical, chemical, and biological factors. Coatings rich in silver have been used to inhibit this undesirable phenomenon. In this study, the antimicrobial properties of several silver-containing products used as a coating in pipelines were det...
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The objective of this study was the development and design of a treatment system at a pilot-plant scale for the remediation of hydrocarbons in industrial wastewater. The treatment consists of a combined approach of absorption and biodegradation to obtain treated water with sufficient quality to be reused in fire defense systems (FDSs). The plant co...
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Aerobic granular sludge (AGS) comprises an aggregation of microbial cells in a tridimen-sional matrix, which is able to remove carbon, nitrogen and phosphorous as well as other pollutants in a single bioreactor under the same operational conditions. During the past decades, the feasibility of implementing AGS in wastewater treatment plants (WWTPs)...
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Enterobacteriaceae is present in various niches worldwide (i.e., the gastrointestinal tracts of animals, clinical specimens, and diverse environments) and hosts some well-known pathogens (i.e., salmonellas, shigellas and pathogenic coliforms). No investigation has focused on its occurrence in marine salterns, and it is not clear if these hypersalin...
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The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during wastewater treatment leads to concerns about whether this process may represent a focal point for the transmission of COVID-19. An epidemiological analysis, based on a COVID-19 IgG/IgM Rapid Test Cassette, performed on 134 wastewater workers from 59 wastewater trea...
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Two aerobic granular sludge (AGS) sequential batch reactors were operated at a mild (15 °C) temperature for 180 days. One of those bioreactors was exposed to a mixture of diclofenac, naproxen, trimethoprim, and carbamazepine. The AGS system, operating under pressure from emerging contaminants, showed a decrease in COD, BOD5, and TN removal capacity...
Article
Fish-canning wastewater is characterized frequently by a high content of salt (NaCl), making its treatment particularly difficult; however, the knowledge of the effect of NaCl on eukaryotic communities is very limited. In the present study, the global diversity of eukaryotes in activated sludges (AS) from 4 different wastewater treatment plants (WW...
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Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denit...
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The benefits that nitrogen (N) fertilizers represent for the sustenance of the population at the global scale are undeniable. However, the nitrogen-use efficiency (NUE) in agriculture (the amount of nitrogen retrieved in food produced per unit of nitrogen applied) is extremely low. Volatilization, leaching, soil erosion and denitrification processe...
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Bacteria and fungi have been shown to produce nitrous oxide (N2O) during denitrification, but their contribution after nitrate (NO3⁻) application to soil is not clearly established. In a microcosm experiment, the relative contribution of bacteria and fungi to N2O and carbon dioxide (CO2) production by four contrasting soils representing different l...
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Although previous studies have shown that nitrogen (N) fertilization may negatively affect bacterial diversity in bulk soil, information regarding N impacts on the microbial structure and function of both the bulk and rhizosphere soil bacterial microbiome (bacteriome) is less abundant. In this study, tomato and common bean plants, two globally impo...
Article
Partial nitritation was pointed out as the key step to implement the autotrophic nitrogen removal processes at low temperature. This study investigated the initiation and maintenance of a nitritation process with simultaneous COD removal in a sequencing batch reactor (SBR) run at 15 °C and fed with primary settled urban wastewater characterized by...
Article
A pilot-scale anammox biofilter was set up and operated using reject water in order to investigate its nitrogen removal performance and the microbial community present at different stages of operation of this technology. A novel material “Filtralite” was used a support in order to achieve shorter start-up periods and obtain higher nitrogen load rem...
Article
The Aznalcóllar accident, which occurred in 1998, spilled 36 × 10⁵ m³ of pyritic sludge and 9 × 10⁵ m³ of acidic water around an area of 43 km² in the south of Spain. This spill is considered one of the most important metal-mining associated accidents worldwide. In this study, two soil remediation techniques were evaluated: the addition of marble s...
Article
Pharmaceutical active compounds (PhACs) are molecules used for the treatment of physiological disorders that can generate environmental imbalances when discharged into wastewater. In this work, the use of Penicillium oxalicum XD-3.1 as bioremediation tool for the elimination of PhACs in non-sterile real hospital wastewater in batch bench scale bior...
Article
Granular aerobic sludge systems have been a very efficient technology in urban and industrial wastewater treatment. In this research, a novel modification of aerobic granular sludge technology was developed for the treatment of nitrate-polluted groundwater, adding very low concentrations of a solution based on carbon and oligoelements in the ground...
Article
The abundances of the napA, narG, nirK, nirS, norB, nosZI and nosZII genes, involved in all the steps of the bacterial denitrification pathway, were monitored by a quantitative PCR approach in 4 municipal wastewater treatment plants (WWTPs). The absolute and relative abundances of narG were greater than those of napA in all the surveyed facilities,...
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In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an i...
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Non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics are two of the most employed drug groups around the world due to their use in the treatment of edema and pain. However, they also present an ecological challenge because they are considered as potential water pollutants. In this work, the biodegradation of four NSAIDs (diclofenac, ibupro...
Article
Nitrification and denitrification are known to co-occur in soils, but the effect of fertilisation history on N2O fluxes and the relative source partitioning of the N2O has not been thoroughly investigated. In this study, we therefore combined a high-tech 15N stable isotope tracing technique with quantitative PCR (qPCR) to explore the relative contr...
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Wastewaters polluted with hydrocarbons are an environmental problem that has a significant impact on the natural ecosystem and on human health. Thus, the aim of this research was to develop a bioreactor sorbent technology for treating these polluted waters. A lab-scale plant composed of three 1-L bioreactors with different sorbent materials inside...
Chapter
Assessment of the abundance of fungi in environmental samples by quantitative PCR (qPCR) of community DNA is often a difficult task due to biases introduced during PCR amplification, resulting from the differences associated with length polymorphism and the varying number of copies of the rRNA operon among fungal species, the lack of specificity of...
Article
Biofouling significantly reduces the performance efficiency of membrane bioreactors due to the colonization and accumulation of a matrix of microorganisms. In order to investigate the effect of ultrasonic frequency on biofilm formation and bacterial ecology at different frequencies, four microfiltration membrane modules were subjected at 0, 20, 30...
Article
Three lab-scale sequential batch reactors were operated under aerobic granular sludge technology for 150 days. The reactors were inoculated with mild temperature-adapted activated sludge from Granada wastewater treatment plant (WWTP) in order to evaluate the microbial dynamics, the granulation process and the performance of the systems under low, m...
Article
Three bioreactors were inoculated with Polar Arctic Circle-activated sludge, started-up and operated for 150 days at 8, 15 and 26 °C. Removal performances and granular conformation were similar at steady-state, but higher stability from start-up was found when operating at 8 °C. Important changes in the eukaryotic and prokaryotic populations caused...
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The effect of the combined application of urease and nitrification inhibitors on ammonia volatilization and the abundance of nitrifier and denitrifier communities is largely unknown. Here, in a mesocosm experiment, ammonia volatilization was monitored in an agricultural soil treated with urea and either or both of the urease inhibitor N‐(n‐butyl) t...
Article
The communities of Bacteria, Archaea and Fungi in a full-scale moving bed biofilm reactor in Rukatunturi (Ruka, Finland, in the Polar Arctic Circle) were analyzed in order to observe their ecological role in the operation of this system at low temperature. The bioreactor achieved efficient removal performances organic matter (> 96%) and for ammoniu...
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In a pilot plant under the stable‐stage of the two‐stage anaerobic digester, the dynamic of the archaeal, bacterial and eukaryal community structure was studied as well as their link to performance and biogas production. An effective synergic action between microorganisms was reached since biogas production and particularly methane (CH4) generation...
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In a 3-year microcosm study the nitrous oxide (N2O) emission from an agricultural soil amended with urea, ammonium sulphate or potassium nitrate were examined. Soil samples were taken every year to determine the soil physicochemical properties, the total abundance of bacteria, archaea, nitrifier and denitrifier communities and the changes in the st...
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Pseudomonas aeruginosa is an opportunistic human pathogen causing infections in a variety of plant and animal hosts. The gene mcpB, part of the chemosensory gene cluster II, encodes a soluble chemoreceptor whose function remains unknown. Previous studies show that the cheB2 gene, also located in the chemosensory cluster II, is involved in a specifi...
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Halomonas variabilis strain W10, which was isolated from deepsea water samples, grows and produce exopolymeric substances (EPSs) under a wide range of salinity conditions. However, the characteristics of polymers synthesized varied with salt concentrations. In this study, a production index (PI) reference system to characterize the quality of EPSs...
Article
The abundances of total and metabolically active populations of Candidatus Microthrix and Fungi were evaluated by quantitative PCR (qPCR) and retrotranscribed qPCR of ribosomal molecular markers in three different full-scale wastewater treatment plants (WWTPs), in absence of bulking/foaming episodes. Significant differences of the abundance of rDNA...
Article
Biostimulation of the activity of degrading autochthonous microbiota constitutes one of the most accepted bioremediation tools for oil-polluted soils. Applications of nutrients such as nitrogen fertilizers, oleophilic fertilizers or surfactants are some of the strategies employed. Soil microcosm experiments were performed in open glass containers t...
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The nitrous oxide and molecular N emissions from 5-cm length subsamples taken from 20-cm length sample corers containing eutric Cambisol soil fertilised either with urea, ammonium or nitrate for 1 year have been examined using gas chromatography. At the beginning of the incubation, the same N rate (260 kg N/ha) was added to the soil and kept consta...
Article
The system performance, biomass kinetics and microbial community structure in biofouling and suspended biomass of a hybrid MBBR-MBR system operating at 6 h of hydraulic retention time subjected to four different scenarios of salinity wastewater -constant 6.5 mS cm⁻¹, and tidal-like variable at 4.5, 6.5 and 8.5 mS cm⁻¹ was studied. The performance o...
Article
The abundance of fungi in a full-scale membrane bioreactor (MBR) treating urban wastewater and experiencing seasonal foaming was assessed by quantitative PCR (qPCR), comparing three different sets of widely used universal fungal primers targeting the gene encoding the small ribosomal subunit RNA, 18S-rDNA, (primers NS1-Fung and FungiQuant) or the i...
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Waste treatment and the simultaneous production of energy have gained great interest in the world. In the last decades, scientific efforts have focused largely on improving and developing sustainable bioprocess solutions for energy recovery from challenging waste. Anaerobic digestion (AD) has been developed as a low-cost organic waste treatment tec...
Article
Organic amendments have been shown to improve the quality of agricultural soils. Thus, the use of sewage sludge compost tea as a fertilizer can be considered a worthy and environmentally friendly alternative as it also offers the option of recycling sludge at the same time. However, an in-depth knowledge of how the addition of this product affects...
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This study describes the capacity of different sorbents to form stable biofilms under highly hydrocarbon-polluted conditions and the degrading capacity of the microbiota present in the biofilm. With this aim, microcosms were designed in a 1 L beaker with 400 mL of culture medium or polluted wastewater and an amount equivalent of 200 mL of the selec...
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Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in polluted environments and are included in the priority list of toxic compounds. Previous studies have shown that the fungus Penicillium oxalicum, isolated from a hydrocarbon-polluted pond, has a great capability to transform different PAHs in short periods under submerged fermentatio...
Article
The discovery of anaerobic ammonium oxidation processes led to the development of innovative nitrogen removal technologies, which are more cost-effective and environmentally friendly than conventional activated sludge systems. In this study, the bacterial community structure was determined as well as the nitrogen removal efficiencies in four fixed-...
Article
Three aerobic granular sequencing batch reactors were inoculated using different inocula from Finland, Spain and a mix of both in order to investigate the effect over the degradation performance and the microbial community structure. The Finnish inoculum achieved a faster granulation and a higher depollution performance within the first two month o...
Article
The discovering of Anaerobic Ammonium Oxidation (anammox) process led to the development of autotrophic nitrogen removal systems for the treatment of effluents with low C:N rate. The anammox processes provide an efficient way to remove high concentrations of ammonium compounds from industrial and urban wastewater and covert them to dinitrogen. Neve...
Article
BACKGROUND Autotrophic nitrogen removal was evaluated in three bench‐scale bioreactors filled with Filtralite. This configuration is an alternative to the conventional technologies for nitrogen removal. One of the main problems associated with these new technologies, based on anammox (anaerobic ammonium oxidation) processes, is the stability of bio...
Article
The aim of this work was to study the performance and microbial community structure of a polar Arctic Circle aerobic granular sludge (AGS) system operating at low temperature. Thus, an AGS bioreactor was operated at 7, 5 and 3 °C of temperature using a cold-adapted sludge from Lapland. At 5 °C, it yielded acceptable conversion rates, in terms of ni...
Article
Ammonia-oxidizing bacteria (AOB), ammonia oxidizing archaea (AOA) and N2O-reducing denitrifiers were measured by quantitative real-time PCR (qPCR) in activated sludge samples from four full-scale wastewater treatment plants (WWTPs) in South Spain, and their abundances were linked to the generation of N2O in the samples using multivariate analysis (...
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Drought tolerance of plants such as tomato or pepper can be improved by their inoculation with rhizobacteria such as Microbacterium sp. 3J1. This interaction depends on the production of trehalose by the microorganisms that in turn modulate the phyto-hormone profile of the plant. In this work we describe the characterization of metabolic changes du...
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A membrane bioreactor (MBR) and a hybrid moving bed biofilm reactor-membrane bioreactor (hybrid MBBR-MBR) for municipal wastewater treatment were studied to determine the effect of salinity on nitrogen removal and autotrophic kinetics. The biological systems were analyzed during the start-up phase with a hydraulic retention time (HRT) of 6 h, total...