Jéssica S. Stefano

The production of electrochemical devices and systems using additive manufacturing technology, particularly three‐dimensional (3D) printing, has proven to be highly promising. This work reports the development of 3D‐printed electrochemical sensors for the determination of the antibiotic ciprofloxacin (CIP). To achieve this, a lab‐made conductive fi...

The development of a tailored filament is reported composed of reduced graphene oxide (rGO) and carbon black (CB) in a polylactic acid (PLA) matrix and its use in the production of electrochemical sensors. The electrodes containing rGO showed superior performance when compared with those prepared in the absence of this material. Physicochemical and...

Monkeypox is a zoonotic viral infection caused by MKPV (1). This disease causes skin lesions, fever and headache among other symptoms, which appear within a few days after the infection, and can led to the hospitalization of the infected person (2). Herein, we present the first 3D-printed electrochemical bio-device for multiplex analysis, enabling...

Herein, we present the first 3D-printed electrochemical portable biodevice for the detection of monkeypox virus (MKPV). The electrochemical device consists of two biosensors: an immunosensor and a genosensor specifically designed for the detection of the protein A29 and a target DNA of MKPV, respectively. The electrodes were manufactured using lab-...

Free access until March 29th via the link https://authors.elsevier.com/c/1iZZRvn2M7MFS. Carbon materials are widely used as electrodes because their versatility allows the manufacture of different electrochemical sensors. Chemical vapor deposition (CVD) is a versatile route for their production, allowing carbon growth on different substrates, such...

In this chapter, we explored the use of 3D printing technology applied for the creation of apparatus and devices capable of assisting the detection of viruses. Here, a brief contextualization regarding the recent COVID-19 pandemic is provided, followed by different approaches employing 3D printed tools in the combat of viral diseases, with a focus...

In this chapter, we explore the different electrochemical devices for point-of-care application, as well as the most relevant results regarding the application of these biosensors for sensing the SARS-CoV-2 virus. The different designs and materials used for the development of these prototypes will be explored, as well as the analytical characteris...

This work presents a new additive manufacturing electrochemical device with conductive graphene and polylactic acid (PLA) filament and its application for epinephrine sensing. A three-electrode configuration based on a screen-printed electrode architecture and an easy-to-connect connector was designed. The sensor surface was chemically treated with...

Resorcinol (RS) is a phenolic compound widely used in industrial segments, being used mainly as a disinfectant and antiseptic for skin care. However, this compound can be extremely toxic for both the environment and humans. Thus, the search for new analytical strategies to monitor this compound is of paramount importance. Therefore, the present wor...

Electrochemical sensors based on carbonaceous nanomaterials are widely used for the most diverse purposes, due to the excellent qualities that such nanomaterials present. Considering the different carbonaceous nanomaterials commonly used in electrochemical sensors, nanodiamonds have stood out due to their unique characteristics. Among them, the out...

The use of pesticides is one of the primary means of protecting crops. However, this class of compounds can be highly toxic to the environment and humans. In this aspect, developing analytical devices for monitoring pesticides such as carbendazim in food sources is of paramount importance. Thus, the present work presents the application of a paper-...

Pesticides are heavily employed compounds protecting crops, however, these compounds can be extremely harmful to human health. Once the monitoring of pesticides in foods is of great importance, in this work we propose a ready-to-use electrochemical sensor made with 3D printing technology, capable of detecting paraquat and carbendazim in sequential...

The development of a homemade carbon black composite filament with polylactic acid (CB-PLA) is reported. Optimized filaments containing 28.5% wt. of carbon black were obtained and employed in the 3D printing of improved electrochemical sensors by fused deposition modeling (FDM) technique. The fabricated filaments were used to construct a simple ele...

Analytical assurance of coffees’ geographical indication (GI) authenticity is essential for producers and consumers. In this way, chemometric methods, electrochemical techniques, and 3D printed sensors become attractive to assure the coffee’s quality. These sensors are low-cost, fast, and simple, with the possibility of miniaturization and portabil...

Natural green compounds for sensor modification (binders) are challenging in electrochemistry. Starch is a carbohydrate biopolymer that has been used extensively in the development of biomaterials for the food industry due to its ability to impart textural characteristics and provide gelling or film formation. In particular, the excellent film-form...

A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was explored to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. For that, a simple, non-enzymatic electrochemical sensor, based on a Au-modified G-PLA platform was applied. The AuPs deposited on the electr...

The interest in sustainable and low-cost materials for the production of new sensing platforms has been increasing over time. The possibility of obtaining reliable sensors, on a large scale, easy-to-produce, and cost-effective allows for the development of analytical devices with adequate characteristics for point-of-care analysis. In this context,...

Trace metals such as lead and mercury can be present in beverages by contamination during production and storage processes, or by the use of hops and brewing grains that may be contaminated with trace metals. Since these metals are harmful to human health, their presence is strictly controlled by regulatory agencies. In this aspect, the present wor...

The 3D printing (or additive manufacturing, AM) technology is capable to provide a quick and easy production of objects with freedom of design, reducing waste generation. Among the AM techniques, fused deposition modeling (FDM) has been highlighted due to its affordability, scalability, and possibility of processing an extensive range of materials...

The 3D printing technology has gained ground due to its wide range of applicability. The development of new conductive filaments contributes significantly to the production of improved electrochemical devices. In this context, we report a simple method to producing an efficient conductive filament, containing graphite within the polymer matrix of P...

Electrochemistry combined with economical and sustainable platforms (such as paper) provides portable, affordable, robust, and user-friendly devices. In general, techniques such as photolithography and sputtering are excellent alternatives for producing these platforms. However, due to the requirement of expensive and sophisticated instrumentation...

The growth in the demand for mass analysis, in a fast and safe way, with good reliability and low cost has aroused great scientific interest in the search for new devices that prioritize precision and time. In this sense, this work presents a simple, disposable, and easy-to-use electrochemical sensor, developed on a waterproof paper substrate with...

1-benzylpiperazine (BZP) is an illegal synthetic drug commonly used in combination with alcoholic beverages for recreational purposes due to its amphetamine-like stimulant and hallucinogenic effects. In this work, we described a simple, portable and low-cost electrochemical method for BZP determination in beverage (vodka, whisky and white wine) and...

Viruses are the causing agents for many relevant diseases, including influenza, Ebola, HIV/AIDS, and COVID-19. Its rapid replication and high transmissibility can lead to serious consequences not only to the individual but also to collective health, causing deep economic impacts. In this scenario, diagnosis tools are of significant importance, allo...

Viruses are the causing agents for many relevant diseases, including influenza, Ebola, HIV/AIDS, and COVID-19. Its rapid replication and high transmissibility can lead to serious consequences not only to the individual but also to collective health, causing deep economic impacts. In this scenario, diagnosis tools are of significant importance, allo...

In this chapter the main classes of nanomaterials that have being explored very recently for sensing purposes are discussed. This selection includes classifications according to chemical, structural, and morphological features, from which the reader will be able to differentiate metallic and magnetic nanoparticles, quantum dots, polymeric nanomater...

This work presents a novel procedure involving the sequential chemical treatment to generate reduced graphene oxide (rGO) within 3D-printed polylactic acid (PLA) electrodes and their potential applications for sensing and biosensing. A new configuration of a compact all-3D-printed electrochemical device containing the three electrodes is presented,...

This communication demonstrates that a 3D printing pen, commercially-available as a toy for kids, can be used to draw thermoplastic electrochemical sensors using conductive filaments over any desirable format and substrate. A polylactic acid filament containing graphene or carbon black was passed through the 3D pen that works as a handheld 3D print...

Chlorhexidine (CRX) is the active substance in many antiseptic products and its main hydrolytic degradation product is 4-chloroaniline (4-CA), a compound with carcinogenic potential. This work presents a new electrochemical method for the determination of low concentrations of 4-CA in CRX-based antiseptic samples. Preliminary investigations using e...

The fabrication of carbon black/polylactic acid (PLA) electrodes using a 3D printing pen is presented and compared with electrodes obtained by a desktop fused deposition modelling (FDM) 3D printer. The 3D pen was used for the fast production of electrodes in two designs using customized 3D printed parts to act as template and guide the reproducible...

This communication shows the electrochemical synthesis of Prussian blue (PB) films on additive manufactured (3D-printed) electrodes from iron impurities found at the graphene-polylactic acid (G/PLA) substrate and its application as highly selective sensor for H2O2. The 3D-printed G/PLA electrode was immersed in dimethylformamide for 30 min to expos...

A bipolar electrochemistry setup for the sensitive indirect detection of redox active analytes by means of a fluorescence signal generated by the oxidation of dihydroresorufin is proposed. The redox conversion leads to the in situ and real time formation of the oxidized form resorufin, a highly fluorescent molecule. A photomultiplier tube is used f...

This work presents new insights on the electrocatalytic reduction of 2,4,6-trinitrotoluene (TNT) on carbon nanotubes (CNTs)-modified electrodes (multi-walled carbon nanotubes and double-walled carbon nanotubes). Cyclic voltammetry showed at least 5-fold current increase in the electrochemical reduction of TNT on GCE modified with pristine (“as rece...

This work presents a single analytical system able to perform high‐throughput determinations of different pharmaceutical molecules on screen‐printed electrodes (SPEs) assembled on a batch‐injection analysis (BIA) cell. Two types of SPEs, both containing a carbon conductive ink as working electrode, were selected for the determination of levamisole...

Flexible graphite sheets or graphite paper are conductive substrates produced in large scale for use in a wide range of applications, such as in telecommunications, lighting, computer and peripherals, power conversion, and heat generating semiconductors (low-cost material). In this paper we show, for the first time, that commercially available grap...

Levamisole is the most common adulterant found in cocaine samples and its electrochemical determination in cocaine seized samples is a challenge due to peak overlapping with cocaine. Herein, we propose a deconvolution procedure for levamisole determination in seized cocaine samples using screen‐printed carbon electrodes (SPE). Square‐wave voltammet...

The determination of antioxidant capacity of food samples using disposable polyester screen-printed graphitic macroelectrodes (SPGE) coupled with a batch-injection cell based on the measurement of the consumption of 2,2-diphenyl-1-picrylhydrazyl (DPPH.) is reported. The SPGEs are organic-resistant electrodes and thus compatible with food samples an...

This work demonstrates that the electrochemistry of different molecules of pharmaceutical interest, including omeprazole, ciprofloxacin, dopamine, catechol, hydroquinone, and the antioxidants tert-butylhydroquine, gallic acid, propyl gallate, and pyrogallol, is affected by the functionalization of multi-walled carbon nanotubes (MWCNTs). Voltammetri...

This work demonstrates the sensitive amperometric determination of omeprazole on screen-printed electrodes (SPE) using batch-injection analysis (BIA). A SPE containing a multiwalled carbon nanotube modified electrode coupled to the BIA system provided an analytical method with a low detection limit (9 nmol L− 1), high precision (1.3%) and sample th...

This article highlights the potential use of multi-walled carbon-nanotube modified screen-printed electrodes (SPEs) for the amperometric sensing of ciprofloxacin and compares the association of batch-injection analysis (BIA) and flow-injection analysis (FIA) with amperometric detection. Both analytical systems provided precise (RSD<5 %) and sensiti...

The electrochemical oxidation of naproxen was investigated at a multiwalled carbon nanotube (MWCNT)-modified electrode. A decrease (200 mV) in the overpotential of the naproxen oxidation reaction and considerable (4-fold) current increase (compared to the bare glassy-carbon electrode) was observed. Two one-electron transfers were verified at both b...

This work reports the simultaneous determination of paracetamol and naproxen using multiple-pulse amperometric detection on a multi-walled carbon nanotube modified screen-printed electrode (MWCNT-SPE) adapted in a homemade flow-injection analysis (FIA) cell. A sequence of two potential pulses (+0.30 and +0.70 V for 70 ms each) was applied continuou...

The electrochemical oxidation of chlorhexidine is investigated for the first time and its amperometric determination using a flow-injection analysis (FIA) system is demonstrated. An oxidation peak was observed at around 1.3 V in a 0.1 mol L-1 HClO4 solution at a glassy-carbon electrode. The mechanism of the electrochemical oxidation of chlorhexidin...

A novel and fast electroanalytical method for naproxen determination in pharmaceutical formulations using batch injection analysis (BIA) with pulsed amperometric detection is described. Bare glassy carbon electrode was used as working electrode and 0.05 mol L-1 phosphate buffer solution as supporting electrolyte. The amperometric method involved th...