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29
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Introduction
Jes Dreier currently works at the Department of Applied Physics, KTH Royal Institute of Technology.
Current institution
Publications
Publications (29)
Super-resolution fluorescence microscopy allows for unprecedented in situ visualization of biological structures, but its application to materials science has so far been comparatively limited. One of the main reasons is the lack of powerful dyes that allow for labeling and photoswitching in materials science systems. In this study it is shown that...
In this study we use the combination of super resolution optical microscopy and raster image correlation spectroscopy (RICS) to study the mechanism of action of liposomes as transdermal drug delivery systems in human skin. Two different compositions of liposomes were applied to newly excised human skin, a POPC liposome and a more flexible liposome...
The orientational texture of gel-phase lipid bilayers is a phenomenon that can structure membrane domains. Using two-photon polarized fluorescence microscopy and image analysis, we map the lateral variation of the lipid orientation (the texture) in single domains. With this method, we uncover a lipid-induced transition between vortex and uniform te...
Nanostructured metal films may exhibit localized plasmon resonances that are of great interest due to their potential use in sensors and other photonic devices. In this Letter, we employ a general and versatile method for fabricating metal films with imprinted cavities using a combination of colloidal lithography, template stripping, and plasma etc...
Sex hormones are essential modulators of birdsong. Testosterone, and its active androgenic and estrogenic metabolites, 5a-dihydrotestosterone (DHT) and estradiol, can re-shape the brain circuits responsible for song learning and production. The differential mechanisms of action of these different hormones during song development and song maintenanc...
When euryhaline fish move between fresh water (FW) and seawater (SW), the intestine undergoes functional changes to handle imbibed SW. In Japanese medaka, the potential transcellular aquaporin-mediated conduits for water are paradoxically downregulated during SW acclimation, suggesting paracellular transport to be of principal importance in hyperos...
Complex motor skills take considerable time and practice to learn. Without continued practice the level of skill performance quickly degrades, posing a problem for the timely utilization of skilled motor behaviors. Here we quantified the recurring development of vocal motor skills and the accompanying changes in synaptic connectivity in the brain o...
RESOLFT fluorescence nanoscopy can nowadays image details far beyond the diffraction limit. However, signal to noise ratio (SNR) and temporal resolution are still a concern, especially deep inside living cells and organisms. In this work, we developed a non-deterministic scanning approach based on a real-time feedback system which speeds up the acq...
Significance
Basement membrane is a particular kind of sheet-like extracellular matrix that separates tissue compartments. Invasion of cells through basement membrane barriers is a key aspect of many normal and pathological processes, including organ development and cancer cell metastasis. Invasive protrusions are rich in actin, a cytoskeletal biop...
Complex motor skills take considerable time and practice to learn. Without continued practice the level of skill performance quickly degrades, posing a problem for the timely utilization of skilled motor responses. Here we quantified the recurring development of vocal motor skills and the accompanying changes in synaptic connectivity in the brain o...
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversib...
Reversibly photoswitchable fluorescent proteins (RSFPs) admirably combine the genetic encoding of fluorescence with the ability to repeatedly toggle between a bright and dark state, adding a new temporal dimension to the fluorescence signal. Accordingly, in the last years RSFPs have paved the way to novel applications in cell imaging that rely on t...
Understanding the penetration properties of substances across biological barriers and membranes is vital for many areas of research. In the case of human skin, the barrier is primarily found in the stratum corneum and consists of protein-enriched cells surrounded by a lipid membrane -enriched intercellular spaces. Characterization of the structural...
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The principles governing the in-plane organization of biomembranes remains enigmatic more than 20 years after the proposition of the raft hypothesis.The recent discovery of orientational texture of membrane gel domains represents a previously hidden level of membrane complexity[1]. Using polarized two-photon fluorescence imaging we have shown that...
The tilted gel phase of lipid bilayers can display in-plane orientational texture due to long-range alignment of the molecular director. We explore systematic variations of texture defects in a series of binary phospholipid membranes. Using polarized two-photon fluorescence microscopy the texture pattern of single domains is revealed. The appearanc...
Important aspects of lateral organization in biomembranes can be addressed in model systems. Recently, it has become clear from polarized fluorescence imaging and X-ray scattering that gel domains in bilayers may contain orientational texture related to the tilted acyl chains. Such internal structure of domains can be imaged in polarized 2-photon f...
The properties of water molecules at the interface of lipid bilayers are different compared to the properties of bulk water. For example, in bulk water each molecule is hydrogen bonded with four neighboring water molecules on average, whereas this number is changed at the interface. The structure of water next to lipids was studied previously using...
We propose a simple and reproducible method for fabricating large area metal films with inter-connected nanostructures using a combination of colloidal lithography, metal deposition and a template stripping technique. The method is generic in the sense that it is possible to produce a variety of nanostructures and the corresponding inverted nanostr...
