Jerzy Kawiak

Jerzy Kawiak
Polish Academy of Sciences | PAN · Nałęcz Institute of Biocybernetics and Biomedical Engineering

Doctor of Medicine

About

195
Publications
9,133
Reads
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1,586
Citations
Citations since 2017
11 Research Items
449 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
Introduction

Publications

Publications (195)
Article
Full-text available
Introduction: Standard treatment for chronic lymphocytic leukemia (CLL) has experienced a dramatic change over the last few years. Until recently, CLL was treated using chemoimmunotherapy (CIT) with anti-CD20 monoclonal antibodies. Even though novel agents such as BTKi (Bruton Tyrosine Kinase inhibitor) and BCL2 inhibitors are the standard of care...
Article
Full-text available
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response. Whether the magnitude of HSPCs impairment in sepsis is severity-dependent remains unknown. This stu...
Article
Full-text available
Sepsis remains a major challenge in translational research given its heterogeneous pathophysiology and the lack of specific therapeutics. The use of humanized mouse chimeras with transplanted human hematopoietic cells may improve the clinical relevance of pre-clinical studies. However, knowledge of the human immuno-inflammatory response during seps...
Article
Full-text available
Septic shock is associated with multiple injuries to organs and tissues. These events may induce the regenerative response of adult stem cells. However, little is known about how endogenous stem cells are modulated by sepsis. This study analyzed the circulation of hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs) and very small e...
Article
Full-text available
Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vei...
Data
The example of real time proliferation assay result of stimulated and controlled HUVECs directly from the X-Celligence system. The difference between proliferation curves for cells in culture with presence of hAM CCM and in control medium was not significant for up to 100 h observation. (TIF)
Chapter
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Cryopreservation is a method which enables to store the cells for a long time period and allows to obtain the appropriate amount of cells necessary for transplantation. Unfortunately, the cells isolated from organs like e.g. hepatocytes are susceptible to freezing damage. Encapsulation may be considered as a method allowing to protect cells during...
Article
Full-text available
Here we present the concept of making own patient’s anti-cancer treatment more efficient and starting at testing the efficacy of immunological system. The respective tests are suggested, with special attention devoted to tumour-induced microenvironmental changes. The tumour should be considered to represent a complex tissue in which the cancer cell...
Article
Full-text available
Various processes, taking place both in cells and in their environment, are linked to carcinogenesis. This paper aims at recalling the complex mechanisms of oncogenesis, with particular attention paid to responses of the immune system. In development of solid tumours, leukaemias and lymphomas several common stages can be noted. A neoplastic disease...
Article
Robust detection of bacteria can significantly reduce risks of nosocomial infections, which are a serious problem even in developed countries (4.1 million cases each year in Europe). Here we demonstrate utilization of novel multifunctional bioconjugates as specific probes for bacteria detection. Bifunctional magnetic-fluorescent microparticles are...
Article
Human endothelial cells are used in experimental models for studying in vitro pathophysiological mechanisms of different diseases. We developed an original bioreactor, which can simulate human blood vessel, with capillary polysulfone membranes covered with the human umbilical vein endothelial cells (HUVECs) and we characterized its properties. The...
Article
Full-text available
An effective immune response to severe bacterial infections requires a robust production of the innate immunity cells from hematopoietic stem and progenitor cells (HSPCs) in a process called emergency myelopoiesis. In sepsis, an altered immune response that leads to a failure of bacterial clearance is often observed. In this study, we aimed to eval...
Article
Full-text available
The skeletal muscle injury triggers the inflammatory response which is crucial for damaged muscle fiber degradation and satellite cell activation. Immunodeficient mice are often used as a model to study the myogenic potential of transplanted human stem cells. Therefore, it is crucial to elucidate whether such model truly reflects processes occurrin...
Article
Introduction: Sepsis deregulates early hematopoiesis and can shift stem cells into an "emergency program", but dynamics of this phenomenon are unclear. We aimed to characterize outcome-dependent changes occurring in the hematopoietic stem and progenitor cells (HSPCs) compartment during acute polymicrobial sepsis. Methods: Forty female CD-1 mice...
Article
Full-text available
Small B-cell lymphocytic lymphoma/chronic lymphocytic leukemia, which typically affects elderly people, is a group of conditions that are not clinically uniform. It has been suggested that using the combined activity of the monoclonal antibody anti-CD20 (rituximab) and Listeria monocytogenes toxin listeriolysin O (LLO) for this condition could prod...
Article
Albumin is rarely used for electrospinning because it does not form fibres in its native globular form. This paper presents a novel method for electrospinning human albumin from a solution containing pharmaceutical grade protein and 25% polyethylene oxide (PEO) used as the fibre-forming agent. After spontaneous cross-linking at body temperature, wi...
Article
An experiment involving active immunotherapy of leukemia in mice and its extension to human patients with chronic lymphocytic leukemia is described. First, anticancer activation of the immune system, how it is achieved, and observations of the central blockade of the immune system in leukemia are discussed. Clinical results of antileukemia immune a...
Article
Full-text available
Background Glycated hemoglobin A1c (HbA1c) has been used as an index of glycemic control in the management, guidance, and clinical trials of diabetic patients for the past 35 years. The aim of this study was to validate the HbA1c model in patients with type 1 and type 2 diabetes and to use it to support interpretation of HbA1c in different clinical...
Article
Full-text available
Efficient fight with infection requires robust production of immunocompetent cells. This response is called emergency hematopoiesis and depends on the proliferation of progenitor cells and awakening dormant hematopoietic stem cells (HSCs) into cycling. As during sepsis an altered immune system is often observed, it seems important to reveal the imp...
Article
Full-text available
In vitro studies have shown that amnion-produced growth factors participated in angiogenesis, re-epithelialization, and immunomodulation. The aim of our study was to investigate the growth factors and receptors produced by human amnion tissue and amniotic cells. Human amnions (hAM) were isolated, and amnion circles were dissected for in vitro analy...
Chapter
Severe infections, sepsis, and septic shock remain a major cause of death in the critically ill patients. Activation of systemic response to infection and disruption of most regulatory axes in the body lead to tissue injury and the development of organ failure. Moreover, reprogramming of the immune system results in the unsuccessful fight with path...
Conference Paper
The main objective of this study was to asses usefulness of usage of transmembrane pressure changes measurements for monitoring of cells culture in the closed construction of bioreactor with semipermeable capillary membranes. Two simultaneous HUVECs’ cultures in bioreactors with an assumed cells densities of 20,000 and 60,000 cells/cm2 were carried...
Article
Full-text available
Background Listeriolysin O (LLO) is the main virulence factor of Listeria monocytogenes and facilitates the intracellular survival of the pathogen. Some of its characteristics endorse the growing popularity of LLO for use in biotechnology, particularly in the development of novel vaccines. Here, we evaluate the use of LLO to eradicate leukaemia cel...
Article
Full-text available
Encapsulation of cells in nano-thin polymeric shells allowing for separation of biological material from produced factors may find application in the systems for biological processes regulation. The purpose of the study was to demonstrate the usability of the constructed membrane with the incorporated fullerenol, its performance and detection as a...
Article
Purpose: The objective was to assess glucose, lactate, glycerol, and pyruvate concentrations in the interstitial fluid of the adipose tissue as well as the glucose relative recovery coefficient in reference to capillary blood (RC) during the first two days of the standard treatment of diabetic ketoacidosis (DKA) in patients with type 1 and type 2...
Article
Full-text available
There are few methods for quantifying cell proliferation. Those tests describe the proliferation kinetics of a cell population, but they do not report the history of single cells, the number and frequency of cell divisions, or the precursor cell frequency. Cell-tracking assays based on dilution of the green fluorescent protein labelling dye, CFSE,...
Conference Paper
Glycated hemoglobin A1c (HbA1c) has been the gold standard index of the metabolic control in the diabetes treatment for 30 years. The goal of this study was to estimate the overall glycation rate constant (k) in a simple mathematical model of HbA1c formation for patients with type 1 and type 2 diabetes based on the mean glycemia calculated using th...
Article
Full-text available
Lung tumors are characterized by their high metastatic potential, which is the main cause of therapeutic failure. However, the exact cellular origin of metastasis remains unknown. Since the introduction of the cancer stem cell theory, lung cancer stem cells (LCSCs) have been thought to represent metastasis-founding cells. The current study aimed to...
Article
Full-text available
Different sources of stem cells are considered as a potential source of precursor cells that could participate and improve skeletal muscle regeneration. Under physiological conditions regeneration is based on the satellite cells, i.e. adult muscle precursor cells that are localized between muscle fiber and surrounding basal lamina. These cells rema...
Article
Encapsulation of cells in polymeric shells allowing for separation of biological material from produced factors may find application in the systems for biological processes regulation. Inadequate efficiency of existing therapeutic anticancer regiments and the rise of multi-drug resistant cancer cells have required investigations into novel anticanc...
Article
Full-text available
The leading pathophysiological changes during sepsis include systemic abnormalities in the immuneresponse. Due to the general character of these disturbances, sepsis is usually studied as a homogenous clinicalcondition. We aimed to compare the immune response in intraabdominal sepsis (IAS) and pneumonia-derivedsepsis (PDS). The following cell popul...
Article
Full-text available
Bacterial toxins can exhibit anticancer activities. Here we investigated the anticancer effects of the listeriolysin O toxin produced by Listeria monocytogenes. We found that supernatants of Listeria monocytogenes strains (wild type, 1189, and 1190) were cytotoxic to the Jurkat cell line and human peripheral blood mononuclear cells (PBMC) in a conc...
Article
Full-text available
Non-hematopoietic cord blood cells and mesenchymal cells of umbilical cord Wharton's jelly have been shown to be able to differentiate into various cell types. Thus, as they are readily available and do not raise any ethical issues these cells are considered to be a potential source of material that can be used in regenerative medicine. In our prev...
Article
To avoid destruction of the implanted biological material it may be separated from host immunological system by enclosure within a permiselective membrane. Two-directional diffusion through the membrane of nutrients, metabolic products, as well as bioactive products of encapsulated cells is required to ensure their survival and functional activitie...
Article
Full-text available
Encapsulation of biological material in the permiselective membrane allows to construct a system separating cells from their products, which may find biotechnological as well as biomedical applications in biological processes regulation. Application of a permiselective membrane allows avoiding an attack of the implanted microorganisms on the host....
Article
Full-text available
The Polish histochemical community lost a meritorious member, when Prof.Józef Niweliński passed away in Kraków on May 16, 2010.
Article
The objectives were as follows: (1) estimating mean value of the overall hemoglobin glycation rate constant (k); (2) analyzing inter-individual variability of k; (3) verifying ability of the hemoglobin A1c (HbA1c) formation model to predict changes of HbA1c during red blood cells cultivation in vitro and to reproduce the clinical data. The mean k e...
Article
Full-text available
The life span of adult cells is restricted, becoming senescent and eventually undergoing apoptosis. Cells are replaced by new ones which originate from a population of stem cells. An example is provided by the hemopoietic system in the bone marrow. In an adult body, stem cells may be identified by markers present in both embryonic and tissue-specif...
Article
The occurrence of chronic myeloid leukemia (CML) is related to the appearance of reciprocal chromosomal translocation (9;22)(q34;q11). As a result of translocation between chromosomes 9 and 22, shortened chromosome 22 - Philadelphia (Ph) chromosome arises, coding for constitutively active protein tyrosine kinase, BCR-ABL1. The subsequent deregulati...
Article
In the present study we investigated in vivo therapeutic potential of DCs vaccines in B-cell chronic lymphocytic leukemia (B-CLL). On the day 0 the SCID mice were intraperitoneally inoculated with peripheral blood mononuclear cells (PBMC) of B-CLL patients at a dose of 10-30 x 10(6) and left untreated (controls) or i.p. injected on the day 7 with 0...
Article
The encapsulation of bacteria may be used to harness them for longer period of time in order to make them viable, while antibiotic treatment would result in controlled release of therapeutic molecules. Encapsulated bacteria Escherichia coli GFP (green fluorescent protein) (E. coli GFP) were used here as a model for therapeutic substance - GFP fragm...
Article
Full-text available
Small cell lung cancer constitutes 15-20% cases of lung cancers, currently the leading cause of death from malignant diseases. It also causes the demise of >90% of affected individuals in 5 years. We have established a new SCLC cell line STP54 derived from fine needle aspirate of metastatic supraclavicular lymph node of 54 -year-old women for model...
Article
Full-text available
Small cell lung cancer constitutes 15-20% cases of lung cancers, currently the leading cause of death from malignant diseases. It also causes the demise of >90% of affected individuals in 5 years. We have established a new SCLC cell line STP54 derived from fine needle aspirate of metastatic supraclavicular lymph node of 54 -year-old women for model...
Article
Full-text available
In the present study we investigated in vivo therapeutic potential of DCs vaccines in B-cell chronic lymphocytic leukemia (B-CLL). On the day 0 the SCID mice were intraperitoneally inoculated with peripheral blood mononuclear cells (PBMC) of B-CLL patients at a dose of 10-30 x 10(6) and left untreated (controls) or i.p. injected on the day 7 with 0...
Article
Full-text available
In this study 10(7) peripheral blood mononuclear cells including on average 78.56% CD19+/CD5+ lymphocytes were irradiated, and then administered intradermally as an anti-cancer vaccine to seventeen patients with B-cell chronic lymphocytic leukemia (B-CLL) at early stages (twelve injections, four at a weekly interval followed by eight vaccines given...
Article
Glycated hemoglobin A1c (HbA1c) concentration in blood is an index of the glycemic control widely used in diabetology. The aim of the work was to validate two mathematical models of HbA1c formation (assuming irreversible or reversible glycation, respectively) and select a model, which was able to predict changes of HbA1c concentration in response t...
Article
The objective of the study was to establish the quasi-continuous courses, using microdialysis technique, of glucose, lactate, and glycerol concentrations in interstitial fluid of abdominal adipose tissue during the standard treatment of acute diabetes complications. Clinical studies were carried out on 31 diabetic patients during the initial 48 h o...
Article
Autologous osteochondral transplantation is one of the methods that can be used to create hyaline or hyaline - like repair in the defect area. The purpose of the present study was to repair the full - thickness articular cartilage defects in nine rabbits' knee joints with autologous cultured chondrocytes. An articular cartilage defect was created o...
Article
The assessment of biocompatibility of systems consisting of a host, permiselective membrane and a biological material, encapsulated within may be standardized for biological material immunoisolation purposes. The procedures for evaluation of the membrane non-toxicity against the biological material, the membrane physical and chemical stability post...
Article
Encapsulation of bacteria in a semipermeable polymer membrane gives a lot of technological possibilities. Our aim was to evaluate the performance of bacteria encapsulated in hollow fibers when treated with chosen antibiotic. The antibiotic application may cause release of biologically active substances for which production the bacteria may be genet...
Article
Implantable bioartificial liver has been investigated for patients suffering from liver insufficiency after mass liver resection or acute liver failure. Liver cells are implanted as free cell suspension, in microencapsulation systems or using microcarriers. To exhibit their typical functions, hepatic cells need a three-dimensional environment that...
Article
Glycated hemoglobin A1c (HbA1c) concentration in blood is an index of the glycemic control widely used in diabetology. The aim of the work was to validate two mathematical models of HbA1c formation (assuming irreversible or reversible glycation, respectively) and select a model, which was able to predict changes of HbA1c concentration in response t...