Jeremías Gastón Galletti

Jeremías Gastón Galletti
National Scientific and Technical Research Council | conicet · IMEX Instituto de Medicina Experimental

MD PhD

About

34
Publications
10,490
Reads
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624
Citations
Citations since 2016
15 Research Items
513 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Additional affiliations
June 2007 - December 2012
Universidad de Buenos Aires
Position
  • Medical Doctor
August 2004 - present
Academia Nacional de Medicina, Buenos Aires
Position
  • PostDoc Position
July 2004 - May 2012
Academia Nacional de Medicina
Position
  • PhD Student

Publications

Publications (34)
Article
Full-text available
Dry eye is a highly prevalent immune disorder characterized by a dysfunctional tear film and a Th1/Th17 T cell response at the ocular surface. The specificity of these pathogenic effector T cells remains to be determined, but auto-reactivity is considered likely. However, we have previously shown that ocular mucosal tolerance to an exogenous antige...
Article
Full-text available
Dry eye is an allegedly autoimmune disorder for which the initiating mechanisms and the targeted antigens in the ocular surface are not known, yet there is extensive evidence that a localized Th1/Th17 effector T cell response is responsible for its pathogenesis. In this work, we explore the reconciling hypothesis that desiccating stress, which is u...
Article
Full-text available
To evaluate intereye corneal asymmetry in Pentacam (Oculus Optikgeräte GmbH, Wetzlar, Germany) indices as a diagnostic method between normal patients and patients with keratoconus. A retrospective, observational case series of 177 healthy, 44 indeterminate, and 121 patients with keratoconus classified by Pentacam ectasia detection indices, randomiz...
Article
Full-text available
Purpose: To evaluate the role of nuclear factor-κB (NF-κB) activation in eye drop preservative toxicity and the effect of topical NF-κB inhibitors on preservative-facilitated allergic conjunctivitis. Methods: Balb/c mice were instilled ovalbumin (OVA) combined with benzalkonium chloride (BAK) and/or NF-κB inhibitors in both eyes. After immunizat...
Article
Full-text available
The impact of topical eye drops with benzalkonium chloride (BAK) as a preservative could involve more than the reported toxic effects on the ocular surface epithelium and ultimately affect the immune balance of the conjunctiva. We found that BAK not only impairs tolerance induction in a murine model, but leads to mild systemic immunization. Contras...
Article
Full-text available
Neurotrophins are a family of closely related secreted proteins that promote differentiation, development, and survival of neurons, which include nerve growth factor (NGF), brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4. All neurotrophins signal through tropomyosin receptor kinases (TrkA, TrkB, and TrkC) which are more select...
Article
Full-text available
Neutrophils play major roles against bacteria and fungi infections not only due to their microbicide properties but also because they release mediators like Interleukin-1 beta (IL-1β) that contribute to orchestrate the inflammatory response. This cytokine is a leaderless protein synthesized in the cytoplasm as a precursor (pro-IL-1β) that is proteo...
Article
As the cornea is densely innervated, its nerves are integral not only to its structure but also to its pathophysiology. Corneal integrity depends on a protective tear film that is maintained by corneal sensation and the reflex arcs that control tearing and blinking. Furthermore, corneal nerves promote epithelial growth and local immunoregulation. T...
Article
Aging is associated with a massive infiltration of T lymphocytes in the lacrimal gland. Here, we aimed to characterize the immune phenotype of aged CD4+ T cells in this tissue as compared with lymphoid organs. To perform this, we sorted regulatory T cells (Tregs, CD4+CD25+GITR+) and non-Tregs (CD4+CD25negGITRneg) in lymphoid organs from female C57B...
Article
The ocular surface is the part of the visual system directly exposed to the environment, and it comprises the cornea, the first refractive tissue layer, and its surrounding structures. The ocular surface has evolved to keep the cornea smooth and wet, a prerequisite for proper sight, and also protected. To this aim, the ocular surface is a bona fide...
Article
Since the last century, advances in healthcare, housing, and education have led to an increase in life expectancy. Longevity is accompanied by a higher prevalence of age-related diseases, such as cancer, autoimmunity, diabetes, and infection, and part of this increase in disease incidence relates to the significant changes that aging brings about i...
Article
Full-text available
Dry eye disease (DED) is a highly prevalent ocular surface disorder with neuroimmune pathophysiology. Tear hyperosmolarity (THO), a frequent finding in affected patients, is considered a key element in DED pathogenesis, yet existing animal models are based on subjecting the ocular surface to the more complex desiccating stress ‐decreased tear produ...
Article
Full-text available
Fever is a hallmark of infections and inflammatory diseases, represented by an increase of 1–4°C in core body temperature. Fever-range hyperthermia (FRH) has been shown to increase neutrophil recruitment to local sites of infection. Here, we evaluated the impact of a short period (1 h) of FRH (STFRH) on pro-inflammatory and bactericidal human neutr...
Article
Full-text available
Objective: Data on treatment outcome for rheg-matogenous retinal detachment (RRD) in Latin American countries is scarce, thus we set out to determine anatomical outcome of surgery and its associated factors in two Argentine clinics. Methods: Retrospective review of patients presenting with primary RRD from 2012 to 2016 at two eye clinics in Buenos...
Article
Full-text available
Immunological interdependence between the two eyes has been reported for the cornea and the retina but not for the ocular mucosal surface. Intriguingly, patients frequently report ocular surface-related symptoms in the other eye after unilateral ocular surgery. Here we show how unilateral eye injuries in mice affect the mucosal immune response of t...
Article
Full-text available
Interleukin-1β (IL-1β), a major pro-inflammatory cytokine, is a leaderless cytosolic protein whose secretion does not follow the classical endoplasmic reticulum-to-Golgi pathway, and for which a canonical mechanism of secretion remains to be established. Neutrophils are essential players against bacterial and fungi infections. These cells are rapid...
Article
Full-text available
The ocular surface is constantly exposed to environmental irritants, allergens and pathogens, against which it can mount a prompt immune response to preserve its integrity. But to avoid unnecessary inflammation, the ocular surface's mucosal immune system must also discriminate between harmless and potentially dangerous antigens, a seemingly complic...
Article
Full-text available
Purpose. To thoroughly analyze corneal deformation responses curves obtained by Ocular Response Analyzer (ORA) testing in order to improve subclinical keratoconus detection. Methods. Observational case series of 87 control and 73 subclinical keratoconus eyes. Examination included corneal topography, tomography, and biomechanical testing with ORA. F...
Article
Full-text available
Abstract Chronic lymphocytic leukemia (CLL) is the main cause of autoimmune hemolytic anemia (AHA). However, the cellular basis underlying this strong association remains unclear. We previously demonstrated that leukemic B cells from CLL patients recognize the erythrocyte protein Band 3, a prevalent autoantigen in AHA. Here we show that the major b...
Poster
Abstract Purpose: To assess the state of conjunctival tolerance in a murine model of evaporative dry eye and to evaluate the effect of topical nuclear factor kappa B (NFkB) modulation on disease progression. Methods: A previously reported murine model of evaporative dry eye (Niederkorn JY et al. J Immunol. 2006 Apr 1;176(7):3950-7) was used with 8...
Article
Full-text available
To evaluate Pentacam ectasia detection indices in topographically-normal patients and in subclinical keratoconus cases. Prospective, observational case series. Setting: institutional; Patients: group 1 comprised one eye from 189 patients with unremarkable topography and groups 2 and 3 included the better and worse eyes, respectively, of 55 keratoco...
Article
Full-text available
To evaluate inter-device agreement between Placido topography (iTrace; Tracey Technologies, Houston, TX) and Scheimpflug tomography (Pentacam; Oculus Optikgeräte GmbH, Wetzlar, Germany) for measuring corneal power and cylinder and axis of astigmatism. Observational case series of 54 eyes from 54 subjects with no ocular disease. Main outcome measure...
Article
Full-text available
To evaluate corneal biomechanical properties in non-keratoconic myopic eyes and to identify descriptors for improving the specificity of the Ocular Response Analyzer (ORA) (Reichert Ophthalmic Instruments, Depew, NY) testing for subclinical keratoconus detection. Observational case series of 52 non-keratoconic non-myopic eyes and 97 non-keratoconic...
Article
Full-text available
Purpose: To report anterior segment optical coherence tomography (AS-OCT) findings in corneas of keratoconus patients treated with riboflavin-ultraviolet-A-light-induced crosslinking (CXL). Methods: Prospective, non-randomized, single-center clinical study that included 9 CXL-treated keratoconus eyes followed with corneal topography, specular micr...
Article
Full-text available
To compare corneal hysteresis (CH) and corneal resistance factor (CRF) between normal eyes and eyes with keratoconus correcting for the effect of central corneal thickness (CCT) and to estimate keratoconus detection sensitivity and specificity of these parameters. Observational case series of 102 normal eyes (control group) and 77 eyes with keratoc...
Article
Full-text available
T cells from patients with chronic lymphocytic leukemia may play an important role in contributing to the onset, sustenance, and exacerbation of the disease by providing survival and proliferative signals to the leukemic clone within lymph nodes and bone marrow. By performing chemotaxis assays towards CXCL12, CCL21 and CCL19, we sought to evaluate...
Article
Full-text available
Resumen objetIvo: Comunicar la presentación conjunta de síndrome de Saethre-Chotzen y queratocono en dos pacientes relacionados. casos clínIcos: Dos hermanos con síndrome de Saethre-Chotzen y patrón ectásico corneal compatible con queratocono seguidos durante años en nuestra División Oftalmología. conclusIones: Si bien la asociación entre ambas pat...
Article
Full-text available
The mechanisms underlying the frequent association between chronic lymphocytic leukemia (CLL) and autoimmune hemolytic anemia are currently unclear. The erythrocyte protein band 3 (B3) is one of the most frequently targeted Ags in autoimmune hemolytic anemia. In this study, we show that CLL cells specifically recognize B3 through a still unidentifi...
Article
Full-text available
The mechanisms underlying the frequent association between chronic lymphocytic leukemia (CLL) and autoimmune hemolytic anemia (AHA) remain ill-defined. In AHA autoantibodies are mostly directed to the erythrocyte proteins band 3 (B3), Rh complex or glycophorins. We explored the possibility that CLL cells could initiate the autoimmune response by pr...
Article
Full-text available
Resumen La leucemia linfática crónica (LLC) se asocia frecuentemente con anemia hemolítica autoinmune (AHA), pero los mecanismos responsables no se han dilucidado todavía. En la AHA los autoanticuerpos están dirigidos mayormente contra las proteínas del eritrocito banda 3 (B3), complejo Rh o glicoforinas. Nosotros investigamos la posibilidad de que...

Questions

Questions (4)
Question
Which percentage of labeled T cells should I find in the peripheral lymph nodes after adoptive transfer of say 5-10 million labeled T cells? Is there a difference in efficiency between the intraperitoneal and intravenous routes? I find intraperitoneal injections to be very reproducible and reliable, whereas tail-vein injections can be quite variable from mouse to mouse. We just transferred 35 million CFSE-labeled CD3+ cells by intraperitoneal injection to 12 mice and we observed between 0.4-0.7% labeled cells in the popliteal and cervical lymph nodes 48 h later. I was expecting to find about 10 times that number...
Question
I´m performing DTH assays in mice. I usually immunize with 100 ug OVA in complete Freund´s adjuvant (1:1 mixture) by a subcutaneous injection, wait for two weeks and then inject 100 ug heat-aggregated OVA in 50 ul in one of the footpads. I also inject the same volume of PBS in the other footpad to control for the unspecific swelling response. There is commonly up to 0.1 mm swelling in the control footpad and at most 0.3 mm swelling in the antigen-loaded footpad. I was hoping to obtain more swelling or at least more reproducible reactions so I don´t have to work with many mice per treatment group. Has anyone tried using 10- to 100-fold higher doses of antigen for the DTH reaction?
Question
I'm evaluating the capacity of some epithelial cell supernatants to induce dendritic cell maturation. I take murine bone-marrow-derived dendritic cells on day 6 (DCs) and culture them for 48 h in the presence of culture medium alone (immature DCs), 1 ug/ml LPS (mature DCs) and my supernatants at 25% concentration. I observe a comparable increase in MHC II and CD86 expression in all treatments but in immature DCs, as expected. I then take the DCs, wash them twice and culture them again at a 1:10 ratio with CFSE-labelled allogeneic T cells. I observe significant proliferation with SN-exposed DCs on day 3 (up to 4-5 rounds of proliferation) and not with LPS (only 2-3 round) at this timepoint. If I wait up to day 5, LPS DCs induce proliferation similar to that observed for the SNs on day 3, and at this point, the SN DC culture have considerable cell death. I cannot conclude from these experiments that SN DCs are more efficient at T cell stimulation, can I? Do you have any suggestions? What do you think is going on in my experimental setup?
Question
I have grade V ovalbumin. I have tried to heat a 20 mg/ml solution at 60 C for 10 min and I ended up with coagulated protein, almost like cooked egg white. Does anyone know of a good protocol for a delayed-type hypersensitivity reaction?

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Projects

Project (1)
Project
To evaluate the feasibility of diverse forms of T cell-based immunotherapy for improving dry eye signs in mouse models of disease