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Publications (115)
Acute Hepatopancreatic Necrosis Disease (AHPND), caused by various species of Vibrio, results in significant mortality rates in global penaeid shrimp aquaculture. While antibiotics are commonly employed to combat this disease, concerns about the rapid emergence of resistant strains and their potential adverse effects on human health and the environ...
Penaeus vannamei is the main species of shrimp farmed worldwide, and for Ecuador it is the first non-oil economic
sector. However, various pathogens, including white spot syndrome virus (WSSV), threaten the sustainability of
shrimp farming. P. vannamei larvae are susceptible to WSSV infection via vertical or horizontal transmission. To
decrease...
Research on bioprospecting bioactive secondary metabolites from marine bacteria has rapidly expanded in the past few decades. The complexity of the environment in which bacterial communities live involves complex interactions with other communities competing for resources for survival. Previously, the ability of Pseudovibrio denitrificans has been...
Research on bioprospecting bioactive secondary metabolites from marine bacteria has rapidly expanded in the past few decades. The complexity of the environment in which bacterial communities live involves complex interactions with other communities competing for resources for survival. Previously, the ability of Pseudovibrio denitrificans has been...
The formation of extracellular traps (ETs) is a cell death mechanism relying on the release of nucleic acids in response to different stimuli. More recently, ETs have been recognized as an important cellular immune response since they are able to entrap and kill various microorganisms. The main goal was to describe a methodology to induce and visua...
The genus Muricea, a highly speciose gorgonian coral group in the Tropical Eastern Pacific (TEP), is commonly found on shallow rocky reefs including Machalilla National Park (MNP), El Pelado Marine Reserve (REMAPE) and Galápagos Marine Reserve (GMR). Here, we report the presence of M. hebes, M. echinata and M. robusta, which have been not previousl...
Vibriosis is a multifactorial disease that causes economic losses in the shrimp farming industry. As part of an ecological approach for its control, aqueous extracts from plants generally recognized as safe (GRAS): Allium sativum (290000 µg mL-1), Camellia sinensis (40000 µg mL-1), Morinda citrifolia (250000 µg mL-1) and Origanum vulgare (50000 µg...
Peptide therapeutics play a key role in the development of new medical treatments. The traditional focus on endogenous peptides has shifted from first discovering other natural sources of these molecules, to later synthesizing those with unique bioactivities. This review provides concise information concerning antimicrobial peptides derived from ma...
Pathogenic vibrios of farmed shrimp have acquired resistance to antibiotics commonly used in aquaculture,
leading to a constant search for new, effective products to control them. In vitro methods currently used to
evaluate possible solutions are expensive, tedious, and time-consuming. In contrast, the methyl thiazole tetra�zolium (MTT) reduction...
Hypoxia is a common concern in shrimp aquaculture, affecting growth and survival. Although recent studies have revealed important insights into hypoxia in shrimp and crustaceans, knowledge gaps remain regarding this stressor at the molecular level. In the present study, a gas chromatography–mass spectrometry (GC–MS)-based metabolomics approach was...
The Equatorial Front, in the southern part of the Tropical Eastern Pacific (TEP) region, has been characterized as a hotspot of functional biodiversity due to the mixing of warm and cold waters. Nevertheless, the biogeographic patterns for some organisms, such as octocorals, remain unknown in some coastal regions. Therefore, we aimed to assess the...
The use of probiotics, prebiotics and dietary fiber has become a common practice in shrimp aquaculture as alternatives to antibiotic treatment. However, not much is known about the metabolic mechanisms underlying the effects of probiotics and immunostimulant used in shrimp aquaculture. In this study, a gas chromatography−mass spectrometry (GC−MS) b...
The vibrios carrying the pirA and pirB toxin genes cause acute hepatopancreatic necrosis disease (AHPND) in cultivated shrimp, significantly affecting the yield. The use of probiotic strains is a promising strategy to prevent vibriosis in shrimp cultures. The microbial consortium formulated with three strains, Vibrio diabolicus (Ili strain), Vibrio...
Background
Acute hepatopancreatic necrosis disease (AHPND) is an important shrimp bacterial disease caused by some Vibrio species. The severity of the impact of this disease on aquaculture worldwide has made it necessary to develop alternatives to prophylactic antibiotics use, such as the application of probiotics. To assess the potential to use pr...
Outbreaks of white spot syndrome virus (WSSV) have caused serious damage to penaeid shrimp aquaculture worldwide. Despite great efforts to characterize the virus, the conditions that lead to infection and the infection mechanisms, there is still a lack of understanding regarding these complex virus-host interactions, which is needed to develop cons...
The first taxonomic descriptions of the sponge diversity at El Pelado Marine Protected Area in the province of Santa Elena, Ecuador is reported. Tedania (Tedania) ecuadoriensis Jaramillo & Hajdu, sp. nov. is described from its shallow waters. In addition, Callyspongia (Callyspongia) aff. californica (sensu Cruz-Barraza and Carballo 2008; non sensu...
The emergence of new virulent Vibrio strains resistant to common antibiotics has caused significant economic losses to shrimp farming worldwide. It is mandatory to adopt new strategies to control shrimp farming related vibriosis. Essential oils (EOs) have several biological properties among of which the quorum sensing (QS) inhibitory activity is ap...
El metaboloma es el conjunto de compuestos orgánicos de bajo peso molecular (metabolitos) pro- ducidos por sistemas biológicos. El antagonismo microbiano es una importante fuerza evolutiva, por lo que el análisis de su metaboloma es una herramienta útil para la búsqueda de nuevas molé- culas con actividad biológica. El objetivo de este trabajo fue...
El cultivo de camarón es una de las principales actividades acuícola a nivel mundial, y para Ecuador representa el primer producto de exportación no petrolero. A pesar de su crecimiento expansivo, la industria camaronera es frecuentemente amenazada por brotes de enfermedades. Recientemente, varios vibrios han sido asociados a la enfermedad de necro...
Distribución biogeográfica de dos géneros de Octocorales (Leptogorgia y Muricea) en el Frente Ecuatorial Continental, determinando macrozonas costeras entendiendo su influencia ambiental en la distribución de especies.
Description of the diversity of ascidians in the Ecuadorian coast, from Bahía de Caráquez to Santa Clara Island (El Oro)
In the search for probiotic bacteria from marine environments to control pathogenic Vibrio of cultivable marine species, this study focused on members of the genus Pseudovibrio. Based on its symbiotic features and antibacterial capacity, the genus Pseudovibrio could be used as probiotic to control vibriosis in shrimp culture. Fortythree isolates of...
In the search for bioactive marine natural products from zoantharians of the Tropical Eastern Pacific, four new tyrosine dipeptides, named valdiviamides A-D (1-4), were isolated from Antipathozoanthus hickmani, and two new tyramine derivatives, 5 and 6, from Parazoanthus darwini. The phenols of all six tyrosine derivatives are substituted by bromin...
Antibiotics have been used massively for aquatic organism production. However, their use should be limited given the problems of increased antibiotic resistance, and new alternatives should be attempted, such as the use of plants extracts with antibacterial properties. The effect of the essential oil of oregano (Origanum vulgare) compared with thre...
The marine biodiversity program of CENAIM-ESPOL focuses on sessile invertebrates from the “‘El Pelado’ marine reserve”. The present study aims at understanding the different hierarchical levels of biodiversity found at the “El Pelado” and to guide biodiscovery initiatives. Generated biodiversity data were systematized in a database (Darwin Core sta...
The first chemical study of the marine sponge Callyspongia cf. californica widely distributed along the coasts of the Tropical Eastern Pacific led to the identification of a new family of amphiphilic derivatives called callyspongidic acids. The four isolated metabolites 1-4 feature a hydrophilic diacid end opposed to both an aromatic moiety and a l...
Two new zoanthamine alkaloids, namely 3-acetoxynorzoanthamine (1) and 3-acetoxyzoanthamine (2), have been isolated from the zoantharian Zoanthus cf. pulchellus collected off the coast of the Santa Elena Peninsula, Ecuador, together with three known derivatives: zoanthamine, norzoanthamine, and 3-hydroxynorzoanthamine. The chemical structures of 1 a...
Two new zoanthamine alkaloids, namely 3-acetoxynorzoanthamine (1) and 3-acetoxyzoanthamine (2), have been isolated from the zoantharian Zoanthus cf. pulchellus collected off the coast of the Peninsula of Santa Elena – Ecuador, together with three known alkaloids zoanthamine, norzoanthamine and 3-hydroxynorzoanthamine. The chemical structures of 1 a...
Zoantharians represent a group of marine invertebrates widely distributed from shallow waters to the deep sea. Despite a high diversity and abundance in the rocky reefs of the Pacific Ocean, very few studies have been reported on the diversity of this group in the Tropical Eastern Pacific coasts. While molecular techniques recently clarified some t...
Despite a large occurrence, especially over the Pacific Ocean, the chemical diversity of marine invertebrates belonging to the order Zoantharia is largely underexplored. For the two species of the genus Antipathozoanthus no chemical study has been reported so far. The first chemical investigation of Antipathozoanthus hickmani collected at the Marin...
Alcyonacea are sessile invertebrates, which can significantly shape the boundary layer in coral reefs and rocky habitats. Ecological aspects in this taxon have been well studied in the Caribbean, Mediterranean, and Indo-Pacific. With few recent exceptions, studies in the Eastern Pacific focused on taxonomy. We present a quantitative assessment of A...
To mitigate the economic losses provoked by disease outbreaks, shrimp producers employ therapeutic additives. However, important issues such as the toxicity of these products on shrimp are not always considered. In vivo toxicity assays require a lot of time and large economic and physical resources. Here, we describe an in vitro procedure to evalua...
The first chemical study of the common species Terrazoanthus onoi, present off the coast of Ecuador, led to the identification of a new family of 2-aminoimidazole alkaloids named terrazoanthines A-C (1-3). Homologues 1 and 2 feature an unprecedented 6-(imidazol-5-yl)benzo[d]imidazole. Acyl substitution pattern and complete configurational assignmen...
Estudios sobre la diversidad de invertebrados marinos existentes en Ecuador es escasa a pesar de ser considerado como uno de los países de mayor biodiversidad en el mundo. Los Zoantharios son un grupo de invertebrados marinos (Filo: Cnidaria, Clase: Anthozoa) que se encuentran ampliamente distribuidos en las regiones tropicales de los Océanos. Son...
Las esponjas marinas constituyen una fuente rica de metabolitos bioactivos con propiedades antibacterianas, antitumorales, antifúngicas, antivirales y antinflamatorias. Sin embargo, no todas estas moléculas son producidas por las esponjas, estudios recientes indican que muchos de estos metabolitos son productos de los microorganismos asociados, ent...
Las esponjas marinas constituyen una fuente rica de metabolitos bioactivos con propiedades antibacterianas, antitumorales, antifúngicas, antivirales y antinflamatorias. Sin embargo, no todas estas moléculas son producidas por las esponjas, estudios recientes indican que muchos de estos metabolitos son productos de los microorganismos asociados, ent...
Las esponjas marinas constituyen una fuente rica de metabolitos bioactivos con propiedades antibacterianas, antitumorales, antifúngicas, antivirales y antinflamatorias. Sin embargo, no todas estas moléculas son producidas por las esponjas, estudios recientes indican que muchos de estos metabolitos son productos de los microorganismos asociados, ent...
El uso de antibióticos en la acuicultura del Ecuador es difícil de controlar y radicar por que los productores buscan formas rentables y efectivas, en los vegetales siempre se han encontrado fuentes de alternativas a estos temas, basado en un uso empírico y atoctono de la zona se ha trasladado la adición de extractos vegetales a los cultivos de cam...
Zoantharians are marine invertebrates (Cnidaria, Anthozoa) that are widely spread throughout the Indo-Pacific Oceans. While several studies have been performed to describe their diversity around the Western part of the Pacific, the Eastern part of this Ocean has been less investigated. According to literature, it has been shown that zoantharian and...
Zoantharians are marine invertebrates (Cnidaria, Anthozoa) that are widely spread throughout the Indo-Pacific Oceans. While several studies have been performed to describe their diversity around the Western part of the Pacific, the Eastern part of this Ocean has been less investigated. According to literature, it has been shown that zoantharian and...
Debido a las variaciones ambientales los sistemas de cultivo constituyen
ambientes estresantes que favorecen la aparición y propagación de
enfermedades infecciosas. Las prácticas de manejo para controlarlas han
involucrado el uso de antibióticos y probióticos, muchas veces de forma
empírica. Nosotros proponemos hacer una revisión profunda de esas p...
The hypothesis that intraspecific competition affects survival and growth during the culture and harvest at extensive/semi-intensive Penaeus vannamei shrimp ponds was evaluated. Thus, the effect of stocking density on the biomass, shrimp average weight, survival, and economic performance during the culture (133 days) and at the harvest of the P. va...
Viral diseases restrict the development of the world shrimp industry and there are few studies on cell response to the presence of viral infections. We performed immunohistochemistry assays to characterize hemocytes subpopulations involved in the immune process occurring in the LO of Litopenaeus vannamei shrimp. Tissue sections of animals that incr...
The main task of this research was to test certain health management strategies for cultured shrimp in order to improve overall production variables (yield, survival rate, weight and feed conversion ratio-FCR). To do so, four thorough studies were conducted with an emphasis on the use of three factors: the probiotic Vibrio alginolyticus (Ili strain...
The main task of this research was to test certain health management strategies for cultured shrimp in order to improve overall production variables (yield, survival rate, weight and feed conversion ratio - FCR). To do so, four thorough studies were conducted with an emphasis on the use of three factors: the probiotic Vibrio alginolyticus (Ili stra...
Conclusiones finales del proyecto “Optimización de estrategias de manejo para incrementar la producción de camarón Penaeus vannamei en piscinas semi-intensivas” En el análisis de datos históricos de densidad de siembra, tamaño de piscina y época de cultivo, se encontró que las mejores producciones se lograron con: densidad de siembra < 8.64 animale...
Debido a las bajas producciones de camarón reportadas en Ecuador por la presencia del WSSV, las estrategias para disminuir la incidencia del virus han sido enfocadas en el manejo de la salud de estos organismos. La hipótesis de este estudio plantea que los probióticos Vibrio hepatarius (P62) y Bacillus sp.(P64), y los -1,3/1,6-glucanos (BG) estimu...
En el presente trabajo se planteó la hipótesis de que las larvas de camarón, son portadoras del WSSV. La enfermedad se desarrollaría en los estanques en función de las condiciones ambientales, especialmente la temperatura. Se utilizaron dos temperaturas ( 24ºC y 31ºC) y -1,3-glucanos en dos niveles, presencia y ausencia en la alimentación Se emple...
Diagnóstico de la producción de camarón de cultivo en las provincias de Guayas, El Oro y Santa Elena (2000-2007). Segunda parte En el número anterior (Cenaim Informa No. 150) reportamos la utilización de información generada con el SAEMA para describir e identificar patrones en la producción y manejo de estanques camaroneros en las provincias de Gu...
Protocolo para uso eficiente de B-glucanos en estanques Después de haber realizado varios experimentos de cultivo de camarón con inmunoestimulación hemos determinado que el rendimiento del cultivo en todas las fases de producción puede ser incrementado combinando 3 elementos; probióticos Vibrio alginolyticus (Ili) durante la larvicultura, manejo de...
The effect of the inclusion of probiotics (Vibrio alginolyticus) and β-1,3/1,6-glucans in Penaeus vannamei larviculture was evaluated by measuring the immune response and survival of shrimp juveniles subjected to white spot syndrome virus (WSSV) challenge tests (per os) and pond culture. Treatments were designed to contrast the probiotic factor (in...
Información sobre el uso de inmunoestimulante en cultivo de camarón con particular referencia a los B-glucanos. Un inmunoestimulante es definido como una sustancia o molécula capaz de alertar el sistema inmune. Por lo general hablamos de paredes microbianas (lipopolisacaridos o LPS, peptidoglicanos y β-glucanos) las cuales son capaces de estimular...
Efecto sobre la respuesta inmune y la supervivencia a desafíos con WSSV de la combinación temperatura inmunoestimulantes. El CENAIM ha realizado varios experimentos de cultivo de camarón con inclusión de inmunoestimulantes en la dieta, entre estos principalmente β-glucanos (BG), y en menor grado peptidoglicanos (PG). En sistemas de dos fases con pr...
Estudio de las cualidades inmunoestimulantes de nuevas bacterias probióticas asociadas al cultivo de LV Estudio de las cualidades inmunoestimulantes de nuevas bacterias probióticas asociadas al cultivo de LV
The objective of this work was to obtain probiotic bacterial strains with immunostimulatory qualities for shrimp. A total of 80 strains were isolated from the hepatopancreas of healthy wild shrimp (30±5 g) collected in Manglaralto-Ecuador. The probiotic effect in vitro was evaluated using the agar diffusion technique. Three strains identified as Vi...
La mancha blanca provocada por el WSSV (Whit e Spot Syndrome Virus, por sus siglas en inglés) constituye hasta el momento la enfermedad más desvastadora para el camarón de cultivo. En este artículo se ha realizado una revisión bibliográfica sobre el virus, la enfermedad que provoca, las armas con las que cuenta el hospedero para enfrentar su agresi...
Inmunoestimulación temprana de camarones LV para inducir un mejor desarrollo del sistema inmune y resistencia al WSSV El objetivo del proyecto ha sido inducir un mejor desarrollo de los tejidos inmunitarios del camarón mediante inmunoestimulación desde la larvicultura. El trabajo constó de 3 fases. La larvicultura, bajo diferentes regímenes de inmu...
Estudio de los Mecanismos Celulares para Eliminar el WSSV Los camarones emplean mecanismos celulares como cápsulas (Momoyama et al., 1994) y fagocitosis (Soderhall et al., 1996) durante las patologías virales, las cuales no manifiestan procesos inflamatorios. También se han detectado células en apoptosis (Granja et al., 2003) y esferoides en el órg...
Mortalities of cultured shrimp, Penaeus vannamei (Boone), induced by white spot syndrome virus (WSSV) have occurred in Ecuador since May 1999. Three epidemiological surveys in Ecuadorian farms were carried out and showed an apparent association between lower temperature and increased mortality rates in commercial ponds. Infected animals showed a re...
EFECTO COMBINADO DE UNA PRECRIA EN HIPERTERMIA E INMUNOESTIMULACION. UNA ALTERNATIVA PARA INCREMENTAR LA PRODUCCION DE CULTIVOS SEMI-INTENSIVOS EN CONDICIONES DE WSSV En 1998, Ecuador alcanzó las máximas producciones de camarón de su historia 153,729 TM (CNA 1999) en una superficie de 175, 000 ha (CNA, 2000), es decir 1,923 lbs/ ha año (769 lbs/cic...
Incremento en la supervivencia al WSSV por inmunoestimulación sería característica familiar LV Algunos estudios de inmunoestimulación en camarones en laboratorio y en cultivos de campo sugieren que éstos, bajo alerta inmunitaria pueden eliminar el virus de la mancha blanca de sus tejidos.
Efecto combinado de una precría en hipertermia e inmunoestimulación Las observaciones pre y post WSSV indican que los primeras semanas de cultivo son las de mayor riesgo y las más importantes de controlar en cualquier estrategia de manejo que pretenda incrementar la producción en sistemas semi-intensivos bajo condiciones de WSSV. La estrategia que...
¿Puede la industria camaronera evaluar las sustancias inmunoestimulantes ofrecidas en el mercado y establecer su uso correcto? La crisis que atraviesa la industria camaronera exige alternativas de manejo que permitan incrementar la resistencia de los animales, la manipulación del sistema inmune por medio del uso de estimulantes se vislumbra como ta...
Inmunoestimulación a 33 ˚C de camarones Litopenaeus vannamei Se ha comprobado que los camarones Litopenaeus vannamei tratados a una temperatura de 33 ˚C presentan resistencia al virus de la mancha blanca (WSSV) y tienen alta supervivencia.
Estudio de la respuesta inmunitaria de camarones Litopenaeus vannamei de familias resistentes al WSSV y el efecto de la adición de ßglucanos La industria camaronera en la búsqueda de estrategias de manejo, tanto compañías como institutos de investigación, han desarrollado programas de selección buscando familias que demuestren ser resistentes a enf...
Efectos combinados de las vitaminas C y E dietéticas en la inmunorespuesta del juvenil Litopenaeus vannamei antes y después de la suplementación con glucanos Se considera que el estado nutricional tiene una influencia importante en la salud y en la capacidad de los animales para resistir enfermedades. Estudios relacionando la nutrición con la funci...
Estudio de la resistencia contra el virus WSSV en PV inmunoestimulado y su posible relación con inmunidad adaptiva
En el presente trabajo se obtuvieron cepas bacterianas probioticas con cualidades inmunostimulantes. Un total de ochenta cepas bacterianas se aislaron del hepatopancreas de camarones silvestres sanos Litopenaeus vannamei capturados en Guayaquil, Ecuador. Las cualidades de las bacterias probioticas versus el patógeno Vibrio harveyi se evaluó in vivo...
WSSV y temperatura, inmunoestimulantes, vitaminas... ¿Cómo se relaciona todo? Los experimentos desarrollados a la fecha indican que a temperatura de 33°C disminuye tanto la prevalencia (porcentaje de animales positivos) como la intensidad de la infección en juveniles y en larvas (Boletín Septiembre 15).
Estudio de las cualidades inmunoestimulantes de bacterias probióticas asociadas al cultivo de Litopenaeus vannamei Aunque la utilización de productos químicos (ej: antibióticos) sea una práctica común para el control de patologías, la tendencia actual es restringir el uso de drogas, evitando los efectos secundarios que ocasiona su uso indiscriminad...
PARA IDENTIFICAR MARCADORES INMUNITARIOS DE RESISITENCIA A VIBRIOSIS ES NECESARIO CONTAR CON PROTOCOLOS ESTANDARIZADOS DE INFECCIONES EN ANIMALES JUVENILES EN PENAEUS VANNAMEI. PARA LO CUAL SE UTILIZO EL PATOGENO VIBRIO VULNIFICUS, CAUSANTE DEL SINDROME DE BOLITAS EN LARVAS, EN ANIMALES JUVENILES DE 3 A 8 GRAMOS POR EL METODO DE INMERSION, PREVIA D...
Evaluación de los ß-glucanos como inmunoestimulantes del sistema de defensa del camarón blanco Litopenaeus vannamei (Boone, 1931) Diferentes observaciones de inmunoestimulación han sido consistentes en mostrar un incremento de la actividad fenoloxidasa (PO) y la actividad antibacteriana. Sin embargo, esta estimulación se ha acompañado de pérdida de...
A spectrophotometric nitroblue tetrazolium (NBT) reduction assay was used to demonstrate the production of superoxide anions (O2−) by haemocytes of the white shrimp Penaeus vannamei. It was found that haemocytes, without receiving an experimental stimulant, showed a rather high background activity. Therefore, optimal parameters (number of haemocyte...
Shrimp farming constitutes an important source of revenue and employment in many developing countries. However, infectious diseases have affected the profitability of the shrimp industry. For this reason, disease prevention is a priority and shrimp immunology has become a prime area of research. In such a perspective, studies into the value of cell...
RESUMEN: Los conocimientos adquiridos en inmunología de vertebrados, señalan a las carencias nutricionales como el factor externo más importante de inmunodeficiencias. Dos bioensayos de similar diseño se realizaron para evaluar el efecto de dietas de diferente calidad sobre la respuesta inmune del camarón Penaeus vannamei e identificar marcadores d...
Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues revea...
We report here the isolation of three members of a new family of antimicrobial peptides from the hemolymph of shrimpsPenaeus vannamei in which immune response has not been experimentally induced. The three molecules display antimicrobial activity against
fungi and bacteria with a predominant activity against Gram-positive bacteria. The complete seq...
We report here the isolation of three members of a new family of antimicrobial peptides from the hemo-lymph of shrimps Penaeus vannamei in which immune response has not been experimentally induced. The three molecules display antimicrobial activity against fungi and bacteria with a predominant activity against Gram-positive bacteria. The complete s...
In order to ensure shrimp aquaculture production, immunology has become a priority in terms of disease control and prevention. Research must be devoted to the identification of anti-infectious effectors at the cellular and molecular levels. In this review, recent results obtained in the shrimp Penaeus japonicus are presented and discussed with refe...
Various haemolymph components of the shrimp Penaeus japonicus were identified and characterised by monoclonal antibodies. Three groups of monoclonal antibodies were raised. Their reactivity to haemocyte types and/or secreted molecules was determined by immunofluorescence and the molecular masses of the antigens were analysed by western-blotting. A...
In the context of infectious diseases in mollusc and shrimp aquaculture, research must be focused on diagnosis for zoosanitary controls but also on obtaining resistant animals. This last strategy depends heavily on the development of knowledge about marine invertebrate immunology. With the establishment of purification protocols for the main invert...
In the present work the hypothesis that the shrimp larvae harbours WSSV at undetectables levels and that the White spot disease (WSD) is developed in ponds depending of the environmental conditions, mainly temperature. Two temperature were evaluated 24ºC and 31ºC as triggers in addition β-1,3-glucans were used at two levels, in the presence and abs...
Durante las infecciones virales no se observan zonas inflamadas con fuerte infiltración de hemocitos y melanización típicas de las infecciones bacterianas. Dos tipos de respuesta celular ante infecciones virales se han reportado en camarón. Infecciones con el virus del síndrome de la mancha blanca (WSSV) se caracterizan por ausencia de respuesta ce...
Diagnóstico de la producción de camarón de cultivo en las provincias de Guayas, El Oro y Santa Elena (2000-2007). Primera parte Un subproducto del Sistema de Alerta Epidemiológico y de Manejo Acuícola (SAEMA) es la colección de datos históricos de producción y de manejo de camaroneras. El presente trabajo utiliza la información generada con el SAEM...
Rol de los canales secretores de las glándulas tegumentales del Camarón en la epidemiología de WSSV La literatura señala que los primeros tejidos en infectarse con el virus de la mancha blanca (WSSV) son los epitelios, la glándula antenal y las branquias. Utilizando un anticuerpo específico hemos detectado señal positiva de WSSV en glándulas tegume...
Altas dosis de ß-glucanos incrementan la talla del camarón L.V.. Resultados de la estación fría seca (verano) Durante el invierno (estación cálidahúmeda) del 2006 se realizó un experimento en la estación experimental de CENAIM (Palmar, Guayas).
