Jennifer M Schubert

• Northeastern University

Instrumental advances in infrared micro-spectroscopy have made possible the observation of individual human cells and even subcellular structures. The observed spectra represent a snapshot of the biochemical composition of a cell; this composition varies subtly but reproducibly with cellular effects such as progression through the cell cycle, cell...

Instrumental advances in vibrational microspectroscopy have made possible the observation of individual human cells and even subcellular structures. The observed spectra represent a snapshot of the biochemical composition of a cell; this composition varies subtly but reproducibly with cellular effects such as progression through the cell cycle, cel...

During the last 15years, vibrational spectroscopic methods have been developed, which can be viewed as molecular pathology methods that depend on sampling the entire genome, proteome, and metabolome of cells and tissues, rather than probing for the presence of selected markers. First, this review introduces the background and fundamentals of the sp...

During the last 15years, vibrational spectroscopic methods have been developed, which can be viewed as molecular pathology methods that depend on sampling the entire genome, proteome, and metabolome of cells and tissues, rather than probing for the presence of selected markers. First, this review introduces the background and fundamentals of the sp...

Recent results suggest that infrared microspectroscopy, coupled to methods of multivariate analysis, can distinguish normal from virally infected cells. This was established for infection of the oral cavity by the herpes simplex virus, which is associated with the outbreak of cold sores, and for infection of the cervix uteri by the human papillomav...

In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compa...

Aim Spectral Cytopathology (SCP) is a novel spectroscopic method for objective and unsupervised classification of individual exfoliated cells. The limitations of conventional cytopathology are well-recognized within the pathology community. In SCP, cellular differentiation is made by observing molecular changes in the nucleus and the cytoplasm, whi...

Spectral cytopathology (SCP) is a novel approach for diagnostic differentiation of disease in individual exfoliated cells. SCP is carried out by collecting information on each cell's biochemical composition through an infrared micro-spectral measurement, followed by multivariate data analysis. Deviations from a cell's natural composition produce sp...

IntroductionMethods Results and DiscussionConclusions AcknowledgmentsReferences