Jennifer Gaughran

Jennifer Gaughran
Dublin City University | DCU · School of Physical Sciences

Doctor of Philosophy

About

24
Publications
3,249
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
263
Citations

Publications

Publications (24)
Article
Full-text available
Hypothesis Functionalized nanoparticles (NPs) offer diverse biomedical applications, but their synthesis is complex, costly, and labour-intensive, particularly when providing for additional functionalization requirements which are a key feature of biomedical applications. Pulsed laser ablation in liquids (PLAL) has previously allowed for the synthe...
Article
Full-text available
Every year, the EU emits 13.4 Mt of CO2 solely from plastic production, with 99% of all plastics being produced from fossil fuel sources, while those that are produced from renewable sources use food products as feedstocks. In 2019, 29 Mt of plastic waste was collected in Europe. It is estimated that 32% was recycled, 43% was incinerated and 25% wa...
Chapter
Advanced flow control such as valving and routing is instrumental in coordinating complex preparative and analytical tasks in an automated fashion on highly integrated rotational “Lab-on-a-Disk” (LoaD) systems. This article reviews various recently developed centrifugal flow control techniques which employ strategically placed functional membrane m...
Conference Paper
By incorporating a set of membranes which selectively dissolve upon contact with aqueous or organic solvents at strategic locations on a disc cartridge, we succeeded to fully automate the solid-phase extraction of nucleic acids by varying the spin rate of a low-cost spindle motor. A solvent-and phase-selective graphene oxide (GO) membrane [1] gover...
Conference Paper
For the first time we utilize the spindle motor intrinsic to centrifugal microfluidic systems to drive a grinding mill for cell lysis in a stator-rotor concept. This mechanical concept particularly enables the lysis of armored cells and, as a further benefit, avoids the addition of chemicals that potentially inhibit subsequent reactions. As a proof...
Article
Full-text available
For the first time, we harness the unique phase-selectivity of chip-integrated graphene oxide (GO) membranes to significantly enhance flow control on centrifugal microfluidic platforms. In this paper, we present novel processes for the assembly of these GO membranes into polymeric microfluidic systems and demonstrate that multilayer GO membranes al...
Conference Paper
We show a novel, centrifugo-pneumatic scheme for solvent-selective routing of organic and aqueous flows based on an integrated, graphene oxide (GO) membrane. We then implement a sequential, multi-reagent protocol geared towards automated solid-phase extraction of nucleic acids with a sequence of washing and elution steps with aqueous and organic li...
Article
Full-text available
This paper investigates the unique properties of multilayer Graphene Oxide (GO) as a barrier selective to solvents, as well as the state of aggregation of fluids. To this end, we developed novel processes for the assembly of GO as membranes into polymeric microfluidic systems. We demonstrate that GO blocks pressurized air and organic solutions whil...
Article
Full-text available
We present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves . Using a bead-based stationar...
Conference Paper
Full-text available
The purity and integrity of RNA after extraction from a biosample like blood are critical for both downstream analysis and development of on-chip diagnostics. We report for the first time on a fully centrifugally automated solidphase purification of RNA on an integrated microfluidic disc with sequential release of on-board reagents. Building on our...
Article
Full-text available
This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample...
Conference Paper
Full-text available
We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-t...
Conference Paper
Full-text available
We present a novel integrated, centrifugo-pneumatic micro-homogenizer (“μHomogenizer”) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RN...
Conference Paper
Full-text available
Sleep apnea is a common sleep disorder in which patient sleep patterns are disrupted due to recurrent pauses in breathing or by instances of abnormally low breathing. Current gold standard tests for the detection of apnea events are costly and have the addition of long waiting times. This paper investigates the use of cheap and easy to use sensors...
Article
Full-text available
This work describes the first use of a wireless paired emitter detector diode device (PEDD) as an optical sensor for water quality monitoring in a lab-on-a-disc device. The microfluidic platform, based on an ionogel sensing area combined with a low-cost optical sensor, is applied for quantitative pH and qualitative turbidity monitoring of water sam...
Article
Full-text available
In this article we introduce a novel technology that utilizes specialized water dissolvable thin films for valving in centrifugal microfluidic systems. In previous work (William Meathrel and Cathy Moritz, IVD Technologies, 2007), dissolvable films (DFs) have been assembled in laminar flow devices to form efficient sacrificial valves where DFs simpl...
Conference Paper
Full-text available
Here we report on a novel, centrifugo-pneumatic valving scheme based on water-dissolvable films. Compared to previous active valving approaches, this valving technique is merely controlled by introducing aqueous solutions to the substrate and allowing for dissolution; therefore lending itself to simple fabrication in microfluidic devices. Furthermo...

Questions

Question (1)
Question
I have gold coated quartz crystals that I want to bind with antibodies to perform an immunoassay. The crystals are quite expensive and I would like to be able to clean the surfaces and reuse them.

Network

Cited By