Jennifer Sorensen Forbey

Boise State University | BSU · Department of Biological Sciences

Host microbial communities (hereafter, the ‘microbiome’) are recognized as an important aspect of host health and are gaining attention as a useful biomarker to understand the ecology and demographics of wildlife populations. Several studies indicate that the microbiome may contribute to the adaptive capacity of animals to changing environments ass...

Large‐scale disturbances, such as megafires, motivate restoration at equally large extents. Measuring the survival and growth of individual plants plays a key role in current efforts to monitor restoration success. However, the scale of modern restoration (e.g., >10,000 ha) challenges measurements of demographic rates with field data. In this study...

Understanding interactions between environmental stress and genetic variation is crucial to predict the adaptive capacity of species to climate change. Leaf temperature is both a driver and a responsive indicator of plant physiological response to thermal stress, and methods to monitor it are needed. Foliar temperatures vary across leaf to canopy s...

Field-based transplant gardens, including common and reciprocal garden experiments, are a powerful tool for studying genetic variation and gene-by-environment interactions. These experiments assume that individuals within the garden represent independent replicates growing in a homogenous environment. Plant neighborhood interactions are pervasive a...

The EPSCoR Postdoc Integration Team was created to foster community between postdoctoral researchers from several EPSCoR institutions (Boise State University, Idaho State University, University of Idaho, University of Nevada-Reno, University of Wyoming) and also postdoctoral collaborators within EPSCoR-funded grants (e.g., Track-I GEM3 and Track-II...

Increased ecological disturbances, species invasions, and climate change are creating severe conservation problems for several plant species that are widespread and foundational. Understanding the genetic diversity of these species and how it relates to adaptation to these stressors are necessary for guiding conservation and restoration efforts. Th...

How intensely animals use habitat features depends on their functional properties (i.e., how the feature influences fitness) and the spatial and temporal scale considered. For herbivores, habitat use is expected to reflect the competing risks of starvation, predation, and thermal stress, but the relative influence of each functional property is exp...

A fundamental question about the ecology of herbivore populations pertains to the relative influence of biotic and abiotic processes on nutritional condition. Nutritional condition is influenced in important, yet poorly understood, ways by plant secondary metabolites (PSMs) which can adversely affect a herbivore's physiology and energetics. Here we...

Congeneric species often share ecological niche space resulting in competitive interactions that either limit co-occurrence or lead to niche partitioning. Differences in fundamental nutritional niches mediated through character displacement or isolation during evolution are potential mechanisms that could explain overlapping distribution patterns o...

Plant communities are composed of complex phenotypes that not only differ among taxonomic groups and habitats but also change over time within a species. Restoration projects (e.g., translocations, reseeding) can introduce new functional variation in plants, which further diversifies phenotypes and complicates our ability to identify locally adapti...

Sagebrush ecosystems (Artemisia spp.) face many threats including large wildfires and conversion to invasive annuals, and thus are the focus of intense restoration efforts across the western United States. Specific attention has been given to restoration of sagebrush systems for threatened herbivores, such as Greater Sage-Grouse (Centrocercus uroph...

An integral part of the biomedical field is drug discovery. Since prehistory, natural products have been a major source of drugs used by humans. However, research into natural products can be hindered by the time and cost of collecting and screening large numbers of randomly selected plant samples. One solution is the development of instrumentation...

Sagebrush identification can be improved by using a relatively easy ultraviolet (UV) light test on specimens. Sagebrush produces a variety of water-soluble polyphenols called coumarins, which fluoresce a blue color under UV light and can help differentiate species, subspecies, and hybrids. We tested 16 different sagebrush taxa (including species an...

Biodiversity science encompasses multiple disciplines and biological scales from molecules to landscapes. Nevertheless, biodiversity data are often analyzed separately with discipline‐specific methodologies, constraining resulting inferences to a single scale. To overcome this, we present a topic modeling framework to analyze community composition...

Remotely sensed land cover datasets have been increasingly employed in studies of wildlife habitat use. However , meaningful interpretation of these datasets is dependent on how accurately they estimate habitat features that are important to wildlife. We evaluated the accuracy of the GAP dataset, which is commonly used to classify broad cover categ...

Avian herbivores face the exceptional challenge of digesting recalcitrant plant material while under the selective pressure to reduce gut mass as an adaptation for flight. One mechanism by which avian herbivores may overcome this challenge is to maintain high activities of intestinal enzymes that facilitate the digestion and absorption of nutrients...

Telomere length dynamics are an established biomarker of health and aging in animals. The study of telomeres in numerous species has been facilitated by methods to measure telomere length by real‐time quantitative PCR (qPCR). In this method, telomere length is determined by quantifying the amount of telomeric DNA repeats in a sample and normalizing...

In the Great Basin, changes in climate and associated fire regimes may alter the density and distribution of shrubs, changing the structure and diet quality of plants in burned areas. We evaluated how the structural and phytochemical characteristics of three-tip sagebrush (Artemisia tripartita) relative to Wyoming big sagebrush (A. tridentata wyomi...

ContextThe amount and composition of phytochemicals in forage plants influences habitat quality for wild herbivores. However, evaluating forage quality at fine resolutions across broad spatial extents (i.e., foodscapes) is challenging. Unmanned aerial systems (UAS) provide an avenue for bridging this gap in spatial scale.Objectives We evaluated the...

Dissections on birds of interest for conservation Development of dissection protocol for field and lab application

The role of telomere dynamics in health and aging has been facilitated by the development of qPCR-based telomere length measurements. However, the widespread application of this approach has been limited because of the challenge of developing appropriate reference primers in non-model organisms with genomes that have not been sequenced. Here we dev...

Human disturbance associated with energy development on winter ranges of migratory mule deer can prompt behaviors of perceived risk in areas near development. We evaluated how perceived risk affected the use of available food near development. Mule deer avoided disturbance at multiple scales, resulting in a loss of otherwise available food near dev...

The availability and quality of forage on the landscape constitute the foodscape within which animals make behavioral decisions to acquire food. Novel changes to the foodscape, such as human disturbance, can alter behavioral decisions that favor avoidance of perceived risk over food acquisition. Although behavioral changes and population declines o...

Sage-grouse are two closely related iconic species of the North American West, with historically broad distributions across sagebrush-steppe habitat. Both species are dietary specialists on sagebrush during winter, with presumed adaptations to tolerate the high concentrations of toxic secondary metabolites that function as plant chemical defenses....

Understanding human use of landscapes is important for managing recreational lands. Predicting human behavior is difficult, however, and methods for assessing human habitat use could benefit from advances in other fields. Habitat selection methods from wildlife ecology provide useful tools for studying such topics. We present a case study of the ad...

Herbivores that forage on chemically defended plants consume complex mixtures of plant secondary metabolites (PSMs). However, the mechanisms by which herbivores tolerate mixtures of PSMs are relatively poorly understood. As such, it remains difficult to predict how PSMs, singly or as complex mixtures, influence diet selection by herbivores. Althoug...

To understand how foraging decisions impact individual fitness of herbivores, nutritional ecologists must consider the complex in vivo dynamics of nutrient–nutrient interactions and nutrient–toxin interactions associated with foraging. Mathematical modeling has long been used to make foraging predictions (e.g. optimal foraging theory) but has large...

Microbial detoxification of plant toxins influences the use ofplants as food sources by herbivores. Stephen's woodrats (Neotoma stephensi) specialize on juniper, which is defended by oxalate, phenolics, and monoterpenes, while closely related N. albigula specialize on cactus, which only contains oxalate. Woodrats maintain two gut chambers harboring...

Dietary specialists often reside in habitats that provide a high and predictable abundance of their primary food, which is usually difficult for other herbivores to consume because of high levels of plant toxins or structural impediments. Therefore, sympatric specialist and generalist herbivores may partition food resources within and among plants....

Microbial detoxification of plant defense compounds influences the use of certain plants as food sources by herbivores. The location of microbial detoxification along the gut could have profound influences on the distribution, metabolism, and tolerance to toxic compounds. Stephen’s woodrats ( Neotoma stephensi ) specialize on juniper, which is heav...

Contemporary techniques predicting habitat suitability under climate change projections often underestimate availability of thermal refuges. Habitat structure contributes to thermal heterogeneity at a variety of spatial scales, but quantifying microclimates at organism‐relevant resolutions remains a challenge. Landscapes that appear homogeneous at...

Small mammals in habitats with strong seasonal variation in the thermal environment often exhibit physiological and behavioral adaptations for coping with thermal extremes and reducing thermoregulatory costs. Burrows are especially important for providing thermal refuge when above-ground temperatures require high regulatory costs (e.g., water or en...

Data and R-code for performing non-linear mixed effects segmented regression on respirometry data.

Environmental temperatures and associated thermoregulatory costs for above- and below-ground microhabitat in the sagebrush steppe.

Temperatures for predicting thermoregulatory costs in each microhabitat and estimating standard errors. This dataset is required to perform the bootstrapping procedure to estimate standard errors of resting metabolic rate.

Metadata (abbreviations and descriptions) for Milling_etal_EnviroTemps.csv and Milling_etal_nlmePYRA.R.

Wyoming big sagebrush (Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young) is the most abundant and widely distributed subspecies of big sagebrush and has been treated through chemical application, mechanical treatments, and prescribed burning in efforts thought to improve habitat conditions for species such as greater sage-grouse (Centroc...

Animals face multiple risks while foraging such as the risk of acquiring inadequate energy from food and the risk of predation. We evaluated how two sympatric rabbits (pygmy rabbits, Brachylagus idahoensis, and mountain cottontail rabbits, Sylvilagus nuttallii) that differ in size, use of burrows, and habitat specialization in the sagebrush-steppe...

When animals select habitats, they integrate a suite of behaviors that are influenced by multiple competing resource requirements. Resources that influence decisions about habitat use are likely to differ across spatial scales and hierarchical behaviors. At coarser scales, animals are expected to select resources that are critical to fitness, and a...

Both temperature and diet quality play an important role in the time and energy budgets of small mammalian herbivores. However, little is known about how temperature and diet quality interact to influence diet selection, nutrient extraction, and energetics, and how these effects might differ among species. Therefore, we examined the effects of diet...

Assessing habitat quality is a primary goal of ecologists. However, evaluating habitat features that relate strongly to habitat quality at fine‐scale resolutions across broad‐scale extents is challenging. Unmanned aerial systems ( UAS ) provide an avenue for bridging the gap between relatively high spatial resolution, low spatial extent field‐based...

Understanding behavioral responses of animals to the thermal environment is of increasing importance under changing climate regimes. Thermoregulatory behaviors, such as exploitation of thermal refugia or temporal partitioning of activity, can buffer organisms against hot and cold thermal extremes but may conflict with other life history needs. Our...

An increasing number of threats, both natural (e.g. fires, drought) and anthropogenic (e.g. agriculture, infrastructure development), are likely to affect both availability and quality of plants that grouse rely on for cover and food. As such, there is an increasing need to monitor plants and their use by grouse over space and time to better predic...

Radio-transmitters are used widely in wildlife research because they allow researchers to track individual animals and monitor their activity. However, to provide unbiased information about a population, transmitters must be deployed on a representative sample of animals and must not alter the behavior of the individuals. The greater sage-grouse Ce...

GPS telemetry markedly enhances the temporal and spatial resolution of animal location data, and recent advances in micro-GPS receivers permit their deployment on small mammals. One such technological advance, snapshot technology, allows for improved battery life by reducing the time to first fix via postponing recovery of satellite ephemeris (sate...

Triclocarban (TCC) and triclosan (TCS), two of the most used antimicrobial compounds, can be introduced into ecosystems by applying wastewater treatment plant biosolids to agricultural fields. Concentrations of TCC and TCS were measured in different trophic levels within a terrestrial food web encompassing land-applied biosolids, soil, earthworms (...

Sagebrush (Artemisia spp.) habitat in the Intermountain West is one of the most endangered ecosystems in North America due, in part, to fire, climate change, and anthropogenic disturbances. However, restoration efforts rarely consider the dietary quality of sagebrush that is conserved or restored despite growing evidence that it is an influential p...

Understanding habitat use by animals requires understanding the simultaneous tradeoffs between food and predation risk within a landscape. Quantifying the synergy between patches that provide quality food and those that are safe from predators at a scale relevant to a foraging animal could better reveal the parameters that influence habitat selecti...

One function of the gut microbiota gaining recent attention, especially in herbivorous mammals and insects, is the metabolism of plant secondary metabolites (PSMs). We investigated whether this function exists within the gut communities of a specialist avian herbivore. We sequenced the cecal metagenome of the Greater Sage-grouse (Centrocercus uroph...

When consuming plants, herbivores must deal with both low nutritional quality from cell wall constituents and potentially toxic plant secondary metabolites, which are often inversely related. Herbivores that consume a highly nutritious, but chemically defended plant, may consume high levels of toxins that require energy for detoxification. Alternat...

Small herbivores face risks of predation while foraging and are often forced to trade off food quality for safety. Life history, behaviour, and habitat of predator and prey can influence these trade-offs. We compared how two sympatric rabbits (pygmy rabbit, Brachylagus idahoensis; mountain cottontail, Sylvilagus nuttallii) that differ in size, use...

When selecting habitats, herbivores must weigh multiple risks, such as predation, starvation, toxicity, and thermal stress, forcing them to make fitness trade-offs. Here, we applied the method of paired comparisons (PC) to investigate how herbivores make trade-offs between habitat features that influence selection of food patches. The method of PC...

Sagebrush (Artemisia spp.) in North America is an abundant native plant species that is ecologically and evolutionarily adapted to have a diverse array of biologically active chemicals. Several of these chemicals, specifically polyphenols, have antioxidant activity that may act as biomarkers of biotic or abiotic stress. This study investigated the...

oxygenated monoterpenes (borneol, camphor, and 1,8-cin-eole) inhibited digestive enzymes of both bird species. Camphor and 1,8-cineole inhibited enzymes from chickens more than from sage-grouse. Extracts from both species of sagebrush had similar inhibition of chicken enzymes, but did not inhibit sage-grouse enzymes. These results suggest that spec...

Heterogeneous vegetation structure can create a variable landscape of predation risk—a fearscape—that influences the use and selection of habitat by animals. Mapping the functional properties of vegetation that influence predation risk (e.g., concealment and visibility) across landscapes can be challenging. Traditional ground-based measures of pred...

For foraging herbivores, both food quality and predation risk vary across the landscape. Animals should avoid low-quality food patches in favour of high-quality ones, and seek safe patches while avoiding risky ones. Herbivores often face the foraging dilemma, however, of choosing between high-quality food in risky places or low-quality food in safe...

For herbivores, nutrient intake is limited by the relatively low nutritional quality of plants and high concentrations of potentially toxic defensive compounds (plant secondary metabolites [PSMs]) produced by many plants. In response to phytochemical challenges, some herbivores selectively forage on plants with higher nutrient and lower PSM concent...

Necklace-style radiotransmitters are widely used in galliform research and management, but their influence on galliform flushing behavior has not been evaluated. We evaluated the hypothesis that greater sage-grouse (Centrocercus urophasianus) with necklace-style radiotransmitters are more likely to flush later within flocks than are their non-colla...

Conservation and management of habitat is central to the conservation of grouse. Identifying thresholds of biotic and abiotic risks that may reduce habitat quality is therefore a component of habitat management of grouse. We propose that dietary phytochemicals, specifically plant secondary metabolites (PSMs), represent an ecological risk to grouse,...

We welcome the recognition that self-medication in animals is widespread ("Self-medication in animals," J. C. de Roode et al. , Perspectives, 12 April, p. [150][1]). However, we disagree with the assumption that learning is only a valid explanation of self-medication for animals with high cognitive

We describe some recent themes in the nutritional and chemical ecology of herbivores and the importance of a broad pharmacological view of plant nutrients and chemical defenses that we integrate as "Pharm-ecology". The central role that dose, concentration, and response to plant components (nutrients and secondary metabolites) play in herbivore for...

Animal habitat selection is a process that functions at multiple, hierarchically. structured spatial scales. Thus multi-scale analyses should be the basis for inferences about factors driving the habitat selection process. Vertebrate herbivores forage selectively on the basis of phytochemistry, but few studies have investigated the influence of sel...

Pygmy rabbits (Brachylagus idahoensis) are one of only three vertebrates that subsist virtually exclusively on sagebrush (Artemisia spp.), which contains high levels of monoterpenes that can be toxic. We examined the mechanisms used by specialist pygmy rabbits to eliminate 1,8-cineole, a monoterpene of sagebrush, and compared them with those of cot...

The plant secondary metabolite papyriferic acid (PA) deters browsing by snowshoe hares (Lepus americanus) on the juvenile developmental stage of the Alaska paper birch (Betula neoalaskana). However, the physiological mechanism that reduces browsing remains unknown. We used pharmacological assays and molecular modeling to test the hypothesis that in...

Nutrient acquisition is a major context for ecological interactions among species but ecologists and nutritionists have developed theory in isolation from each other. Developments in agent-based modelling, state–space modelling of nutrition and multi-scale modelling of landscape ecology provide the components for a new synthesis in nutritional ecol...

A central goal in understanding the ecology and evolution of animals is to identify factors that constrain or expand breadth of diet. Selection of diet in many animals is often constrained by chemical deterrents (i.e., secondary metabolites) in available food items. The integration of chemistry and ecology has led to a significant understanding of...

Understanding dietary specialization in herbivores has theoretical and practical implications in ecology, yet defining niche breadth consistently has been problematic. To increase clarity and communication among ecologists and among disciplines (i.e., chemists, pharmacologists), we propose a specialization key for mammalian herbivores that assigns...

Within our lakes, streams, estuaries, and oceans, there is an astounding chemodiversity of secondary metabolites produced by microbes, algae, and invertebrates. Nearly 30 years of study have yielded hundreds of examples in which secondary metabolites alter the foraging behavior or fitness of aquatic consumers, or both. However, our understanding of...

We propose that the exploitation of the bioactive properties of secondary metabolites (SMs) by animals can provide a "treatment" against various challenges that perturb homeostasis in animals. The unified theoretical framework for the exploitation of SMs by animals is based on a synthesis of research from a wide range of fields and although it is f...

The whitethroat woodrat (Neotoma albigula) eats juniper (Juniperus monosperma), but the amount of juniper in its diet varies seasonally. We tested whether changes in juniper consumption are due to changes in ambient temperature and what the physiological consequences of consuming plant secondary compounds (PSCs) at different ambient temperatures mi...