Jelena M. Petrović

Scientific Veterinary Institute "Novi Sad"

African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern region of the country in districts along the country’s bo...

Pantoprazole is a model substance that requires dosage form adjustments to meet the needs of all patients. Pediatric pantoprazole formulations in Serbia are mostly compounded as capsules (divided powders), while in Western Europe liquid formulations are more common. The aim of this work was to examine and compare the characteristics of compounded l...

Contact lens care solutions are necessary for proper hygiene of contact lenses. Numerous microorganisms can be transmitted through them, leading to serious eye infections, while blindness can occur in particularly severe cases. A direct link between ocular infections and contact lens care solutions has been proven. Commercial preparations must meet...

African swine fever (ASF) is currently the most important challenge for domestic pig production worldwide. The virus reached Eurasia in 2007, and is today affecting more than half of European Union member countries. Among Western Balkan countries, Serbia suffered the first case of ASF in a backyard holding in 2019. Since then, numerous outbreaks in...

The large study on Salmonella spp. in the population of wild boars from twelve hunting estates in the South-West Vojvodina, Serbia was conducted with the aim to investigate the prevalence of Salmonella spp. in wild boars and to trace Salmonella sources. The hunting estates had similar epidemiological characteristics, i.e. lowland regions with an in...

In this pilot study, a method for the determination of phthalates in honey was developed. The following phthalates are included: diethyl phthalate, di-isobutyl phthalate, di-n-butyl phthalate, bis (2-Ethylhexyl) phthalate, and di- (n-octyl) phthalate. For the preparation of the samples, the method of liquid-liquid extraction with hexane with an ult...

This paper will discuss the most common causes of poisoning of honey bees and other pollinating insects that occur in the Republic of Serbia and the world as well. Some potential ways of pollinator exposure to different poisons and their intake will be described. The paper will also deal with the methods of testing and assessing toxicity of newly s...

Honey is a very popular food that is often consumed by immunocompromised people, so it is crucial that it is safe. The safety of honey is determined by numerous hazards- the chemical ones like residues of sulfonamides, lindane and PAHs. Honey from our country contains high concentrations of banned antimicrobial substances such as sulfonamides (3.9%...

Nosemosis is a bee disease practically present in all countries of the world. Control of the presence of Nosema apis and Nosema ceranae is of great importance. The consequences for bees will depend on the degree of infection of the bees colony.Weakening of bee colonies, reduction of production possibilities, contamination with bee feces of hives, d...

American foulbrood (AFB) is one of the most important contagious honey bee diseases. In Serbia, it is mandatory to report AFB, and this disease is registered in our country every year. Starting from 2018, active surveillance of the presence of the AFB has been conducted throughout the country. The paper analyzes the data on the occurrence of AFB du...

The origin of the parasites of Trichinella species goes back to very ancient times–as far as the Paleozoic era, and the organism has been present in the territory of today’s Europe for millions of years. The first epidemic in the region of Serbia was reported in Zemun in 1923. Trichinellosis is the most important parasitic food borne disease in our...

The objective of this study was to analyse the content of OHCs and NDL-PCBs in wild boar population living in densely populated flatland region with intensive agricultural production. A total of 82 liver samples were analysed by GC-MS method, revealing the presence of minimum one residue in the liver of the majority of animals (92.7%). Most commonl...

The pig production is considered to be amongst the animal husbandry sectors with the highest antimicrobial usage. In Serbia, antimicrobials have wide application at pig farms as therapeutic but also as a prophylactic treatment. The aim of the research study was assessment of the antimicrobial usage in different stages of pig production in commercia...

Modern livestock production inevitably involves the use of antimicrobial drugs. Adequate application thereof depends on the application of appropriate biosafety measures as well as timely and accurate diagnostics of the diseases. Administration of antimicrobial drugs without previous identification of “zootechnical errors” or relevant laboratory an...

In the Northern part of Serbia, which is part of Pannonian Basin, mute swan (Cygnus olor) population has notably increased over the last few decades. Like other birds from Anatidae family, mute swans are a host of numerous endoparasite species. Th e aim of this study was to acquire the data on identifi cation and prevalence of endoparasites in mute...

Game meat production differs significantly from production of domestic animals meat. Common measures applied in the control of zoonotic pathogens in farm breeding are not always applicable in hunting estates. Thus, the main objective of this study is to present the methods that should be introduced in the hunting estates to assure production of mic...

Th ermophilic Campylobacter spp. are the leading cause of zoonotic enteric disease in Europe and USA. In Serbia, it has an upward trend in human population. Th e disease is usually indirectly transmitted to humans through the consumption of food contaminated by the faeces of infected animals. Th e aim of this paper was to analyze data on the preval...

Th is paper presents the results of chemical composition and microbiological safety testing of dry cured meat products, fermented and semi-dry or pasteurized sausages produced by several meat industries from Herzegovina. In the period from 2016 to 2018, a total of 85 meat products were sampled. Th ese included 20 samples of fermented pork sausages,...

Slavonska šunka is a traditional dry-cured smoked ham. Smoking with open fire commonly results in the accumulation of polycyclic aromatic hydrocarbons (PAH) molecules in tissues. The objective of this research was to assess the types and concentrations of 16 PAHs in 30 samples of traditional dry-cured smoked ham Slavonska šunka. In general, all sam...

The production chain for game meat is specific and differs from the production chain of meat from domestic livestock. The aim of this study is to present the most important hazards potentially associated with wild boar meat consumption in Serbia. The most important organism is Trichinella spp., as the only well-established causative agent of diseas...

Trichinellosis is one of the most important foodborne diseases in the Eastern European countries. The main objective of this study was to investigate the epidemiological patterns of trichinellosis outbreaks that occurred between 2005 and 2016 in Vojvodina, a northern province of the Republic of Serbia. The average incidence was 3.5 per 100,000 inha...

Maximum limits for PAHs content in honey and bee products are not set. The objective of this study was to conduct a market survey of honey for the presence of PAH16 with the aim of detecting potential contamination and health risks for consumers. A total number of 61 honey samples produced in the territory of Serbia were examined. The content of PA...

Maximum limits for PAHs content in honey and bee products are not set. The objective of this study was to conduct a market survey of honey for the presence of PAH16 with the aim of detecting potential contamination and health risks for consumers. A total number of 61 honey samples produced in the territory of Serbia were examined. The content of PA...

The usage of microbiological screening tests is widespread in control of presence of antimicrobial drug residues in meat samples. Screening tests must be capable to detect antimicrobial drug residue of interest and detection limits must comply with MRL (Maximum Residue Limit). The aim of this study was to examine the performance of a microbiologica...

Enteritis campylobacterialis has an increasing trend in Serbia. Human illness usually appears as sporadic case, most commonly in children with obvious seasonality. In registered outbreaks, incriminated food was most frequently poultry meat. Campylobacter is one of the most important food borne pathogens, commonly underreported, mostly because isola...

Background. To control Trichinella, one of the most important food borne parasites, it is necessary to know the biology of the parasite. Scope and Approach. The pathogenicity of Trichinella genotypes mainly depends on its biological characteristics: reproductive capacity index (RCI), infectivity towards different animal species and humans, differen...

This is the first study offering insights into the prevalence of giant liver fluke in the population of red deer in the territory of Serbia. Giang liver fluke (Fascioloides magna, Bassi, 1875) is the most important liver parasite among wild ruminants in Europe, especially in the region of floodplain for-ests along the upper watercourse of Danube ri...

This is the first study offering insights into the prevalence of giant liver fluke in the population of red deer in the territory of Serbia. Giang liver fluke (Fascioloides magna, Bassi, 1875) is the most important liver parasite among wild ruminants in Europe, especially in the region of floodplain forests along the upper watercourse of Danube riv...

Common carp meat was smoked in traditional conditions without filter and with zeolite, granular activated carbon and gravel filters. The aim of this study was to determine the influence of using different filters in traditional smoking conditions on 16 polycyclic aromatic hydrocarbons from the Environmental Protection Agency list (16 US-EPA PAHs)....

Labudovi su divlje ptice vodenih staništa i pripadaju familiji Anatidae, roduAnseriformes. S obzirom na stanište, način života i ishranu, labudovi predstavljajuznačajnu bioindikatorsku vrstu za monitoring zagađivača u prirodi. Hrane seuglavnom vodenim biljkama i zelenim algama koje predstavljaju direktan pokazateljzagađenja životne sredine i vodeni...

Food can be an important route for transmission of parasites to humans. Compared to other foodborne pathogens in Serbia, foodborne (or potentially foodborne) parasites do not get the attention they undoubtedly deserve. The aim of this article is to give an overview of the most important parasitic pathogens that can be transmitted by food, and that...

The aim of this study was to determine the level of heavy metals and PAHs in 57 samples of canned tuna, 25 samples of canned sardines and 16 samples of canned smoked sprouts, collected from supermarkets in Serbia. Heavy metal levels (lead, cadmium, mercury, arsenic, iron, copper and zinc) were determined using an ICP-MS method. Sixteen analyzed PAH...

Methicillin-resistant Staphylococcus aureus is one of the most important human pathogens. Th ese bacteria have the ability to colonize animals and cross species barriers. Th ree major groups of MRSA strains have emerged. Healthcare associated MRSA and community acquired MRSA strains have potential to spread worldwide and oft en persist in hospitals...

To investigate the microbiological conditions of hunted wild boar carcasses and factors that contribute to the microbial carcass contamination, skin and carcass meat swab samples from 210 hunted wild boars were collected from freshly shot animals. The mean aerobic colony counts (ACCs) and Enterobacteriaceae counts on the skin were 5.2 and 3.6 log10...

During 2015/2016, fecal and intestinal samples from live diseased and/or dead pigs with suspected PEDV and/or TGEV and signs of rotavirus infection were collected from in total seven different farrow-to-finish swine farms located in Northern Serbia region (Vojvodina Province). A total of 14 samples (2 pools per farm) of small intestine with fecal c...

Abstract Mycoplasmal pneumonia caused by Mycoplasma hyopneumoniae (Mhyo) is an important respiratory pig disease that causes severe economic losses to swine industry. It is considered to be most common porcine respiratory disease in commercial swine farms, with low mortality but high morbidity. The aim of research was to evaluate the diagnostic pr...

Introduction: Intensifying of swine industry increases the frequency of swine respiratory diseases regardless of applied measures for their control. Occurrence of swine respiratory diseases is dependent on the whole range of factors, including farming system, nutrition, genetic factors, overall herd health status. Aims: The aim of the study was to...

The aim of this study was to determine the level of organochlorine (OC) pesticides in 57 samples of canned tuna and 31 samples of canned sardines in vegetable oil, collected from supermarkets in Serbia. OC pesticides α-HCH, β-HCH, δ-HCH, dichlorodiphenyltrichloroethane (DDT), DDE, DDD, dielderin, endosulfane I, endosulfane II, endosulan sulfate, en...

Histamine levels in fish and fish products are regarded as a primary criterion for food safety, considering the effects of histamine on human health. The aim of this research was to describe a case of massive histamine intoxication in Serbian children and to provide relevant information regarding the presence of histamine in imported marine fish an...

In November and December in the years 2013 to 2015, a migration technique was used to test 590 wild boar carcasses from seven hunting areas in Serbia (along the rivers Danube, Sava, Sutva, Bosut) for the presence of Alaria alata mesocercariae. Mesocercariae were recovered from 61/590 (10.3%) of mixed tissue samples, with a median of one mesocercari...

This study aimed to monitor antimicrobial resistance trends of Salmonella isolated from meat production chains. A total of 5759 samples were examined for Salmonella presence and antimicrobial resistance. Salmonella species were isolated from 86 samples: (1.49%), 41 of these serotypes were S. Enteritidis (47.67%), 24 were S. Infantis (27.91%). In 20...

In this study, the microbiological status of wild boar carcass meat and the likely sources of its contamination were investigated. Wild boar carcasses (125) were analysed for Total Viable (TVC) and Enterobacteriaceae Count (EBC). The mean TVC and EBC on the skin were 3.5 and 2.1 log10 cfu/cm2, whereas higher levels of 4.3 and 2.9 log10 cfu/cm2 were...

This paper briefly describes mechanisms of resistance to fluoroquinolones in several worldwide distributed Salmonella clones. The isolates have acquired multiple resistance traits over the years due to extensive antibiotic treatment both in human and veterinary medicine. Except for the Salmonella Typhimuirum DT104 where effl ux pump appears to play...

Wild animal meat harvesting and processing is signifi cantly different from classical livestock meat production and represents a challenge by itself. Implementation of concept “from forest to fork” encompasses influence of hunting ground ecology, type of hunting, fi eld evisceration of hunted game, meat inspection aft er shooting or transport etc....

Trichinellosis is a parasitic zoonosis caused by larvae of genus Trichinella. Serbia belongs to a group of countries where Trichinella is present in domestic animals, but also in synanthropic and sylvatic animals. The research on trichinellosis that has been carried out in Serbia so far, aimed at reducing the risk of transmission of trichinellosis...

When placing the fish and fish products on the trade it is necessary to pay attention to the presence of zoonotic parasites that can lead to infection of people especially if the fish is consumed raw or undercooked. Epidemiologically the most important are helminths from the group of Nematoda. The most important are Anisaxis spp. and Eustrongylides...

Current data on the incidence of the disease and abundant research strongly indicate that Trichinellosis has been widely present throughout past few decades, and is endemic in some regions of Serbia. Unfavourable epidemiological situation is due to high prevalence of infection sources and inadequate control of home-processed meat and meat products....

A clinical form of sepsis induced by cecal ligation and puncture (CLP) was caused in order to monitor the concentration of enzymes (alanine aminotransferase - ALT, aspartate aminotransferase - AST, lactate dehydrogenase - LDH, amylase and creatine kinase - CK) in the rat blood. Experiments were performed on male Wistar rats, weighing on average 215...

The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed Salmonella Enteritidis PT4 and Salmonella Typhimurium DT104 are among the most pathogenic strains for humans. Recently there have been reports on the prevalence of ST “like” monophasic 4(5),12:i strains in some countries. Vaccination strategy and antimi...

The aim of this paper was to answer some questions important for a qualitative risk assessment in poultry production related to Campylobacter. These steps are described: hazard identification, hazard characterization, exposure assessment and risk characterization. Hazard identification: Campylobacter is important cause of food borne diseases. Hazar...

The paper presents the recent finding of resistance phenotype and distribution of resistant genes in Escherichia Coli (E. coli) isolated from wild life. The resistance distribution was depended on animal species, their natural habitat, and some seasonal variations were also evident. Wild animals residing close to the farms were more often spreaders...

Control of microbiological safety of animal feed is regulated by a law. The regulation is based on methodological standards for estimation of feed quality. Those methods are permanently amended and strive for continuous improvement of microbiological safety of feed. The question remains whether the presence or absence of some bacterial species is s...

Plasmid mediated resistance to quinolones in Salmonella enterica is briefly presented. World wide spread of qnr determinants is evident, indicating the necessity for prudent use of antimicrobials in human and veterinary medicine. In discovering plasmid mediated resistance to quinolones antimicrobial resistance monitoring for β-lactam antibiotics is...

Current most important side effects of antimicrobial drugs usage are veterinary drug residues in edible animal tissues and development of resistance in food borne pathogens. Enrofloxacine and flumequine are fluoroquinolone antimicrobials licensed in Serbia for use in poultry treatment. Elimination of health risk for consumers is usually made by exa...

Antibiotic residue in milk may have harmful consequences for humans and milk technology. In order to prevent adverse effects of residue, nowadays different screening methods are used for qualitative examination of antibiotic residue in milk. The aim of the work was to investigate the presence of antibiotic residue in the samples of milk from differ...

Trichinosis is a parasitic zoonosis that is caused by parasitic larvae of the genus Trichinella. Serbia is among the countries in which T. spiralis is present, in addition to domestic animals, also in synanthropic and sylvatic animals. This paper presents the results of investigations of the spread of trichinosis among certain species of sylvatic a...

The possibilities of use of microbial phytase in maize/soybean meal diets for broilers from 21 to 42 days of age with different dietary total and available phosphorus (TP and AP) levels were investigated. A 22-day experiment was carried out on 240 Arbor Acres broilers of both sexes divided into six groups. The broilers were given diets with 0.43%,...

The goal of this work was to induce clinical sepsis in rats in order to measure the following biochemical parameters (glucoses, triglycerides, cholesterol, total proteins, albumins and creatinine). The experiments were done on 104 male Wistar rats, body weight 190 to 240 g. The rats were divided in four groups. Treated groups consisted of 28 animal...

The pharmaceutical properties of aromatic plants are partially attributed to essential oils (EOs) which are widely used to prevent and treat human diseases [1]. However, little is known about the control of infective animal diseases with EOs. Arcanobacterium pyogenes (AP) is one of the important opportunistic pathogens of the upper respiratory and...

Modern livestock production method involves the use of antimicrobial agents as an effective way to fight against various infections. The effectiveness of drugs depends on their proper and controlled use. A particular problem is bacteria, such as Salmonella, which are important for human and veterinary medicine. The mechanism of resistance developed...

Antimicrobial resistance is a daunting public health threat impacting both human and animal health and it is a cause for concern wherever antimicrobial agents are in use. The usage of antimicrobial drugs in food producing animals could results in significant food safety issue - antimicrobial resistance among zoonotic bacteria in these animals. Resi...

Arcanobacterium pyogenes is an opportunistic pathogen, a causative agent of suppurative infections of organs and tissues in economically important livestock species. Most frequently this bacteria is isolated from inflamed lung lesions in pigs and cattle, in samples of uterine mucus of cows with endometritis and milk from cows with clinical mastitis...

A new regulation on Salmonella control in Serbia has been implemented recently. The main goal is to eliminate two most common serovars, Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) from the poultry farms and to keep the infections caused by these bacteria under control. Experimental work conducted in the past decade in our Institute...

The most striking advantage of bacteria residing in biofilm is enhanced resistance to biocide including antibiotics and disinfectants. Biocides are 100% efficient in killing cells in suspension but there are not efficient in destroying bacteria from biofilm community. Mechanisms of increased resistance of bacteria in biofilm to antibiotics is not u...

Recognition of food safety problems brings into focus the importance of primary production. Overuse of antimicrobial drugs in animal breeding is reality it is only a question in what manner and extent it influences on food safety. Use of antimicrobials can cause residues and herby influence safety of meat originating from treated animals. Besides r...

The use of some procedures for in vitro investigation of biofilm formation in bacterial species Staphylococcus aureus, Listeria monocytogenes and Pseudomonas aeruginosa isolated from animals is presented in this paper. Biofilms are formed on polystyrene and stainless steel surfaces. On polystyrene surface S. aureus and L. monocytogenes were examine...

One of the greatest challenges of modern laboratory diagnosis is the selection of methods for fast, highly sensitive and specific detection of the infective agents. This challenge is present also in molecular diagnosing of causative agents in veterinary medicine. An example of molecular detection, the application of molecular diagnostic methods and...

Wide application of antimicorbial agents forces bacteria to utilize specific genes and rearrange genomic structure in order to survive in the environment. In this article lateral gene transfer, mobile genetic elements, plasmid mediated resistance and spontaneous mutators in bacteria are briefly described. This resourceful means, by which microorgan...

Some Staphylococcus aureus strains, identified as causative agents of mastitis in cattle, exhibit the ability of producing a viscous extracellular polysaccharide layer (slime), which is nowadays considered to be a virulence factor, as it promotes bacterial adhesion onto the mammary epithelial cells and protects bacteria from opsonization and phagoc...

The volume of hot dog sausages plays an important role in meat production. Due to their desirable eating properties (softness, juicy, mild taste and smell) they form a group of sausages acceptable for all consumer categories. They are produced according to producer’s specification, which are not the same. Therefore, the differences in quality may b...

Listeria monocytogenes is the causative agent of numerous epidemics and sporadic cases of illness in humans. Food is the principal route of infection. Raw materials of animal and vegetable origin are the potential sources of contamination with this bacterium, particularly the foodstuff undergoing minimal processing procedures. However, in the recen...