Jeffrey J Coleman

Jeffrey J Coleman
Auburn University | AU · Department of Entomology and Plant Pathology

PhD

About

74
Publications
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Publications

Publications (74)
Article
Full-text available
Ice nucleation activity (INA) is the capacity of certain particles to catalyze ice formation at temperatures higher than the temperature at which pure water freezes. INA impacts the ratio of liquid to frozen cloud droplets and, therefore, the formation of precipitation and Earth’s radiative balance. Some Fusarium strains secrete ice-nucleating part...
Article
Fusarium head blight (FHB) is a major disease worldwide on cultivated cereals, caused by several Fusarium species. FHB can cause not only yield reduction but also accumulation of mycotoxins in the grain contaminating the food supply. Much of the earlier research has focused on Fusarium pathogenesis, conditions required for disease development and t...
Article
A group of aggressive pathogens have evolved to colonize the plant xylem. In this vascular tissue, where water and nutrients are transported from the roots to the rest of the plant, pathogens must be able to thrive under acropetal xylem sap flow and scarcity of nutrients while having direct contact only with predominantly dead cells. Nevertheless,...
Article
Full-text available
Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) is a devastating fungus pathogen that causes Fusarium wilt in both domesticated cotton species, Gossypium hirsutum (Upland) and G. barbadense (Pima). Greenhouse and field-based pathogenicity assays can be a challenge due to non-uniform inoculum levels, the presence of endophytes, and varying envir...
Chapter
Characterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been previously accomplished by several methods in Fusarium oxysporum. Here we provide a detailed method to generate a gene mutation mediated by a CRISPR/Cas9 ribonucleoprotein (RNP) complex. The Cas9 RNP...
Chapter
Plants produce low molecular weight compounds with antimicrobial activity in response to microbial attack termed phytoalexins. The first phytoalexin identified was (+) pisatin from pea, and several fungi are able to detoxify pisatin to a less inhibitory compound, including F. oxysporum f. sp. pisi. This detoxification is catalyzed by demethylation...
Chapter
F. oxysporum is a notorious filamentous pathogenic fungus that causes serious problems in agriculture and animal/human health. Knowing how the fungus interacts throughout the course of an infection is necessary to propose an effective control strategy, and consequently the manipulation of the F. oxysporum genome is essential to investigate the mole...
Article
Fusarium avenaceum is a filamentous fungus commonly associated with plants and soil. It is a causal agent of Fusarium head blight (FHB) on maize and small-grain cereals, blights on other plant species, and is one of the very few fungal species known to have ice nucleation activity, i.e., it catalyzes ice formation. Here we report the draft genome o...
Article
Reactive oxygen species (ROS) produced by hosts serve as a general defense mechanism against various pathogens. At the interaction site between the host and pathogen, host cells rapidly accumulate high concentrations of ROS, called the oxidative burst, that damage and kill the invading microbes. However, successful pathogens usually survive in a hi...
Article
Full-text available
Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. Previously (Geiser et al. 2013; Phytopathology 103:400-408. 2013), the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agr...
Article
Full-text available
This article is to alert medical mycologists and infectious disease specialists of recent name changes of medically important species of the filamentous mold Fusarium. Fusarium species can cause localized and life-threating infections in humans. Of the 70 Fusarium species that have been reported to cause infections, close to one-third are members o...
Article
Full-text available
Phosphorus (P) is considered a scarce macronutrient for plants in most tropical soils. The application of rock phosphate (RP) has been used to fertilize crops, but the amount of P released is not always at a necessary level for the plant. An alternative to this problem is the use of Phosphorus Solubilizing Microorganisms (PSM) to release P from che...
Article
Full-text available
Fusarium oxysporum is a cross-kingdom fungal pathogen that infects plants and humans. Horizontally transferred lineage-specific (LS) chromosomes were reported to determine host-specific pathogenicity among phytopathogenic F. oxysporum. However, the existence and functional importance of LS chromosomes among human pathogenic isolates are unknown. He...
Article
Full-text available
BACKGROUND: Protoporphyrinogen oxidase (PPO) with two isoforms, chloroplast-targeted (PPO1) and mitochondrial-targeted (PPO2), catalyzes a step in the biosynthesis of chlorophyll and heme. PPO1 and PPO2 are herbicide target sites of PPO-inhibiting herbicides. Target-site mutations conferring resistance to PPO inhibitors have all thus far been in PP...
Article
Full-text available
Fusarium oxysporum f. sp. vasinfectum (Fov) is an important plant pathogen responsible for vascular wilt disease on cotton. Members of this group are known to carry supernumerary chromosomes that encode virulence factors. We sequenced the genomes of five Fov isolates including the genome of a representative of the highly virulent genotype race 4, a...
Article
Full-text available
Widely distributed in various environmental niches, filamentous fungi play an important role in industry, drug development, and plant/animal health. Manipulation of the genome and the coding sequences are essential for a better understanding of the function of genes and their regulation, but traditional genetic approaches in some filamentous fungi...
Article
Full-text available
Fungal infections are a worldwide problem associated with high morbidity and mortality. There are relatively few antifungal agents, and resistance has emerged within these pathogens for the newest antifungal drugs. As the fungal cell wall is critical for growth and development, it is one of the most important targets for drug development. In this r...
Article
Fusarium oxysporum is an economically important pathogen that widely exists in the environment and is capable of causing serious problems in crop production and animal/human health. One important step for characterization of a fungal protein with an unknown function is to determine its subcellular localization within the cell. To facilitate the stu...
Article
Francisella tularensis is a highly infectious Gram-negative intracellular pathogen that causes tularemia. Because of its potential as a bioterrorism agent, there is a need for new therapeutic agents. We therefore developed a whole-animalCaenorhabditis elegans-F. tularensispathosystem for high-throughput screening to identify and characterize potent...
Article
Full-text available
Aim: Caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE), the major constituent of propolis, is able to increase the survival of the nematode Caenorhabditis elegans after infection with the fungal pathogen Candida albicans. Results: CAPE increases the expression of several antimicrobial proteins involved in the immune response to C....
Article
Full-text available
Unlabelled: The intrinsic resistance of Enterococcus faecium to ceftriaxone and cefepime (here referred to as "cephalosporins") is reliant on the presence of class A penicillin-binding proteins (Pbps) PbpF and PonA. Mutants lacking these Pbps exhibit cephalosporin susceptibility that is reversible by exposure to penicillin and by selection on ceph...
Article
Full-text available
Taxonomy: Kingdom Fungi; Phylum Ascomycota; Class Sordariomycetes; Order Hypocreales; Family Nectriaceae; Genus Fusarium. Host range: Members of the FSSC collectively have a very broad host range, and have been subdivided previously into formae speciales. Recent phylogenetic analysis has revealed that formae speciales correspond to biologically...
Article
Full-text available
Candida albicans is a ubiquitous fungus, which can cause very serious and sometimes life-threatening infections in susceptible patients. We used Caenorhabditis elegans as a model host to screen a library of C. albicans mutants for decreased virulence and identified SPT20 as important for virulence. The transcription co-activator SPT20 was identifie...
Article
Fusarium species is a ubiquitous fungus that causes opportunistic infections. We present 26 cases of invasive fusariosis categorized according to the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG) criteria of fungal infections. All cases (20 proven and 6 probable) were treated at Massachusetts General Hos...
Article
Candida spp. cause both local and disseminated infections in immunocompromised patients. Bloodstream infections of Candida spp., known as "candidemia," are associated with a high mortality rate (40%), which is mainly attributed to the long diagnostic time required by blood culture. We introduce a diagnostic platform based on T2 magnetic resonance (...
Article
Full-text available
In this letter, we advocate recognizing the genus Fusarium as the sole name for a group that includes virtually all Fusarium species of importance in plant pathology, mycotoxicology, medicine and basic research. This phylogenetically-guided circumscription will free scientists from any obligation to use other genus names, including teleomorphs, for...
Article
Full-text available
Opportunistic fungal pathogens may cause superficial or serious invasive infections, especially in immunocompromised and debilitated patients. Invasive mycoses represent an exponentially growing threat for human health due to a combination of slow diagnosis and the existence of relatively few classes of available and effective antifungal drugs. The...
Article
Full-text available
Currently accepted fungal diagnostic techniques, such as culture, biopsy, and serology, lack rapidity and efficiency. Newer diagnostic methods, such as polymerase chain reaction (PCR)–based assays, have the potential to improve fungal diagnostics in a faster, more sensitive, and specific manner. Preliminary data indicate that, when PCR-based fungal...
Article
Full-text available
Fusariosis is an emerging infectious complication of immune deficiency, but models to study this infection are lacking. The use of the soil nematode Caenorhabditis elegans as a model host to study the pathogenesis of Fusarium spp. was investigated. We observed that Fusarium conidia consumed by C. elegans can cause a lethal infection and result in m...
Article
Recent work suggests that fungal virulence factors important in human disease have evolved through interactions with environmental predators such as amoebae, nematodes, and insects. This has allowed the use of simple model hosts for the study of fungal pathogenesis; specifically, the nematode Caenorhabditis elegans has become a model host to study...
Chapter
This book, inclusive of 22 chapters, describes novel approaches currently used for antimicrobial drug discovery, focusing on agents for use against bacterial and fungal pathogens. Specific chapters discuss: (1) concepts relevant to drug resistance; (2) microbial mechanisms related to efflux pumps and studies on their potential inhibitors; (3) impac...
Article
The pea pathogen Fusarium oxysporum f. sp. pisi is able to detoxify pisatin produced as a defense response by pea, and the gene encoding this detoxification mechanism, FoPDA1, was 82% identical to the cytochrome P450 pisatin demethylase PDA1 gene in Nectria haematococca. A survey of F. oxysporum f. sp. pisi isolates demonstrated that, as in N. haem...
Article
Members of the fungal genus Fusarium are capable of manifesting in a multitude of clinical infections, most commonly in immunocompromised patients. In order to better understand the interaction between the fungus and host, we have developed the larvae of the greater wax moth, Galleria mellonella, as a heterologous host for fusaria. When conidia are...
Article
Full-text available
BACKGROUND: Candida can cause mucocutaneous and/or systemic infections in hospitalized and immunosuppressed patients. Most individuals are colonized by Candida spp. as part of the oral flora and the intestinal tract. We compared oral and systemic isolates for the capacity to form biofilm in an in vitro biofilm model and pathogenicity in the Galleri...
Conference Paper
Background: There is an increase in the fungal infections. Among them, Candida spp. are the fourth most common cause of hospital acquired bloodstream infections. Mortality from candidemia is over 30%, and early diagnosis and specific therapy intervention are very important factors to achieve a desirable prognosis. Unfortunately, the results from bl...
Data
Absence of inhibition of C. albicans growth by C. neoformans supernatent. C. albicans strain DAY185 was grown on YPD media at 30°C and Spider medium at 37°C overnight in the presence of discs containing the supernatant of C. neoformans strain KN99α (CNS) and the heat inactivated supernatant of C. neoformans (HI-CNS) at the indicated concentrations....
Data
Fungal isolates used in this study. (DOC)
Data
Inhibition of C. albicans by heat inactivated A.fumigatus supernatant. C. albicans strain DAY185 was grown on YPD media at 30°C and Spider medium at 37°C overnight in the presence of discs containing heat inactivated supernatent from isolate AF293 (HI-AFS) at the indicated concentrations. (TIF)
Article
Full-text available
Secondary metabolites are well known for their ability to impede other microorganisms. Reanalysis of a screen of natural products using the Caenorhabditis elegans-Candida albicans infection model identified twelve microbial secondary metabolites capable of conferring an increase in survival to infected nematodes. In this screen, the two compound tr...
Article
Full-text available
Fusarium is the second most frequent mold involved in fungal infections and is particularly important among immunocompromised patients. Culture methods and microscopy are still routinely used in clinical laboratories to identify Fusarium spp, and more sophisticated, timely, and effective methods for detecting Fusarium spp. in laboratory samples cou...
Article
Fusarium is a fungal pathogen of immunosuppressed lung transplant patients associated with a high mortality in those with severe and persistent neutropenia. The principle portal of entry for Fusarium species is the airways, and lung involvement almost always occurs among lung transplant patients with disseminated infection. In these patients, the i...
Article
The fungal plant pathogen Nectria haematococca MPVI produces a cytochrome P450 that is responsible for detoxifying the phytoalexin pisatin, produced as a defense mechanism by its host, garden pea. In this study, we demonstrate that this fungus also produces a specific ATP-binding cassette (ABC) transporter, NhABC1, that enhances its tolerance to pi...
Article
Fluconazole is the first line of therapy for the management of candidiasis. However, fluconazole-resistant strains pose an emerging challenge in everyday clinical practice. In this study, we sought to determine whether cumulative length of hospital stay (CLOS) is a predictive factor for the acquisition of non-susceptible Candida strains to fluconaz...
Article
Full-text available
Candida albicans is an opportunistic fungal pathogen capable of life-threatening disseminated infections particularly in immunocompromised patients. Resistance to many clinically used antifungal agents has created a need to identify and develop a new generation of compounds for therapeutic use. A compound screen to identify potential antifungal nat...
Article
Full-text available
Fusarium species are among the most important phytopathogenic and toxigenic fungi. To understand the molecular underpinnings of pathogenicity in the genus Fusarium, we compared the genomes of three phenotypically diverse species: Fusarium graminearum, Fusarium verticillioides and Fusarium oxysporum f. sp. lycopersici. Our analysis revealed lineage-...
Article
The use of invertebrate model hosts to study infection has seen considerable growth over the last few years. Invertebrates such as Caenorhabditis elegans, Drosophila melanogaster, and Galleria mellonella hold promise for the detailed study of host-pathogen interactions. In particular, these models facilitate the characterization of genes that contr...
Article
Full-text available
Candida albicans, the most common human pathogenic fungus, can establish a persistent lethal infection in the intestine of the microscopic nematode Caenorhabditis elegans. The C. elegans-C. albicans infection model was previously adapted to screen for antifungal compounds. Modifications to this screen have been made to facilitate a high-throughput...
Article
Full-text available
The ascomycetous fungus Nectria haematococca, (asexual name Fusarium solani), is a member of a group of >50 species known as the "Fusarium solani species complex". Members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. The current research an...
Data
Ratio of the number of genes in gene families in N. haematococca MPVI versus F. graminearum. Only gene families that had ≥10 members in N. haematococca MPVI were used in the analysis. The number of genes per family was derived from Interpro calls made by the JGI for N. haematococca MPVI, and by the Munich Institute for Protein Sequences (MIPS) for...
Data
Distibution of repeat identity. NC7 is N. crassa, MG5 is M. oryzae, AN1 is A. nidulans, “FG3 Repeats” is F. graminearum and “FS Repeats” is N. haematococca MPVI. (4.09 MB TIF)
Data
The number of chromatin genes in N. haematococca MPVI compared to other fungi. (0.05 MB DOC)
Data
The number of polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS) of Nectria haematococca MPVI compared to other fungi. (0.06 MB DOC)
Data
The CAX (calcium exchanger) transporter clade from select fungal genomes. Maximum parsimony analysis was used to establish the phylogenetic relationship between the ortholog (Nh65123, red box) and the pseudoparalog (Nh101770, blue box) of N. haematococca MPVI. (8.06 MB TIF)
Data
Effect of RIP on a family of telomere-associated helicases (TAH) in N. haematococca MPVI. Partial alignment of the 12 predicted TAH genes (tah), spanning only the first three conserved motifs. The top row shows the predicted translation of the fourth tah gene on scaffold 45 (TAH_45-4). While many mutations occur in the wobble position, note the pre...
Data
Repeat-induced point mutation (RIP) in N. haematococca MPVI. The hygromycin resistance (hph) gene is mutated from G to A at multiple TpG positions (indicated in red) in isolates 370-4 and 370-8. (1.36 MB TIF)
Data
Comparison of genome statistics of several filamentous ascomycete fungi. (0.06 MB DOC)
Data
Gene families that are at least two-fold larger in Nectria haematococca MPVI than in Fusarium graminearum. (0.09 MB DOC)
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The number of ABC transporters in Nectria haematococca MPVI compared to other fungi. (0.05 MB DOC)
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Distribution of repeat elements in the genome of Nectria haematococca MPVI. (0.08 MB DOC)
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Properties of the chromosomes and genes on each chromosome in N. haematococca MPVI. (0.09 MB DOC)
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The protein kinases of N. haematococca MPVI compared to S. cerevisiae. (0.03 MB DOC)
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Carbohydrate-active enzymes in N. haematococca MPVI compared to other fungi. (0.07 MB DOC)
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Distribution of Small Secreted Proteins (SSP) among filamentous Ascomycetes as identified by SignalP. (0.05 MB DOC)
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Properties of the genes of N. haematococca MPVI. (0.03 MB DOC)
Data
The number of cytochrome P450 genes in Nectria haematococca MPVI compared to other fungi. (0.06 MB DOC)
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Number of predicted genes in Nectria haematococca MPVI that contain transcription factor motifs compared to other fungi. (0.10 MB DOC)