Jeferson Luiz Gogola

Federal University of Paraná | UFPR · Department of Chemistry

This paper reports a rapid and sensitive sensor for the detection and quantification of the COVID-19 N-protein (N-PROT) via an electrochemical mechanism. Single-frequency electrochemical impedance spectroscopy was used as a transduction method for real-time measurement of the N-PROT in an immunosensor system based on gold-conjugate-modified carbon...

This study presents a novel approach for the simultaneous detection of ascorbic acid (AA) and dopamine (DA) using an affordable and user-friendly microfluidic device. Microfluidic devices, when combined with electrochemical detectors like screen-printed electrodes (SPEs), offer numerous advantages such as portability, high sample throughput, and lo...

Electrochemical immunosensors are excellent alternatives to prepare portable platforms used for rapid and inexpensive diagnostic of infectious diseases such as the recently emerged COVID-19. Incorporating synthetic peptides as selective recognition layers combined with nanomaterials such as gold nanoparticles (AuNPs) can significantly enhance the a...

In the present work, we report an innovative approach for immunosensors construction. The experimental strategy is based on the anchoring of biological material at screen-printed carbon electrode (SPE) modified with electrodeposited Graphene Quantum Dots (GQD) and polyhydroxybutyric acid (PHB). It was used as functional substract basis for the reco...

In this work, the simultaneous determination of ascorbic acid (AA) and dopamine (DA) was proposed using a simple and inexpensive microfluidic device. Microfluidic devices are totally suitable to couple with electrochemical detectors such as screen-printed electrodes (SPE) and offer many advantages: portability, high sample throughput and low reagen...

This work presents a feasible process for preparation screen-printed electrode (SPE) using a lab-made conductive ink based on cellulose acetate (CAc) and graphite powder (GP) printed on polyvinyl chloride (PVC) sheet. The CAc present in the composition of this SPE is a binding agent for conductive inks with GP, which are relatively low-cost and eas...

Human immunodeficiency virus (HIV) is still considered a pandemic, and the detection of p24-HIV protein has an important role in the early diagnosis of HIV in adults and newborns. The accessibility of these trials depends on the price and execution difficulty of the method, which can be reduced using electrochemical methods by using enzymeless appr...

Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black...

This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The appli...

This report describes the preparation and evaluation of an immunosensor based on chemical modification of gold surface with hantavirus (strain Araucaria) antigen/protein and the antibody detection by electrochemical analysis. The viral nucleoprotein was immobilized onto gold by SAM formation with 3-mercaptopropionic acid (MPA) followed by activatio...

In the present work a modified carbon paste electrode (MCPE) was prepared using mercury ions (Hg2+) supported at a biochar (bio-charcoal) surface obtained from castor oil cake and used for electrode preparation. The proposed electrode was evaluated for preconcentration and determination of zinc(II) ions present in commercial samples (collyrium and...