Jean Molinier

Jean Molinier
Institut de biologie moléculaire des plantes, Strasbourg

PhD Molecular and Cellular Biology

About

55
Publications
8,045
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2,810
Citations
Citations since 2016
24 Research Items
1197 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200

Publications

Publications (55)
Article
Full-text available
The combination of ever-increasing microscopy resolution with cytogenetical tools allows for detailed analyses of nuclear functional partitioning. However, the need for reliable qualitative and quantitative methodologies to detect and interpret chromatin sub-nuclear organization dynamics is crucial to decipher the underlying molecular processes. Ha...
Chapter
Full-text available
Exposure to genotoxic stress leads to the formation of various types of DNA damages that alter genome integrity. Importantly, DNA lesions occur at particular nucleotide sequences and their reactivity is also under the influence of genome compaction and epigenetic marks such as DNA methylation. The DNA repair pathways that are activated in response...
Article
Full-text available
Beyond their key role in translation, cytosolic transfer RNAs (tRNAs) are involved in a wide range of other biological processes. Nuclear tRNA genes (tDNAs) are transcribed by the RNA polymerase III (RNAP III) and cis-elements, trans-factors as well as genomic features are known to influence their expression. In Arabidopsis, besides a predominant p...
Article
Full-text available
Beyond their key role in translation, cytosolic transfer RNAs (tRNAs) are involved in a wide range of other biological processes. Nuclear tRNA genes (tDNAs) are transcribed by the RNA polymerase III (RNAP III) and cis-elements, trans-factors as well as genomic features are known to influence their expression. In Arabidopsis, besides a predominant p...
Article
Full-text available
Ultraviolet (UV) light is a natural genotoxic agent leading to the formation of photolesions endangering the genomic integrity and thereby the survival of living organisms. To prevent the mutagenetic effect of UV, several specific DNA repair mechanisms are mobilized to accurately maintain genome integrity at photodamaged sites within the complexity...
Article
The critical epigenetic features that characterize remethylable and non-remethylable loci in Arabidopsis determine the capacity of RNA-directed DNA methylation (RdDM)-targeted genomic regions to form stable epialleles.
Preprint
Full-text available
Beyond their key role in translation, cytosolic transfer RNAs (tRNAs) are involved in a wide range of other biological processes. Nuclear tRNA genes (tDNAs) are transcribed by the RNA polymerase III (RNAP III) and cis-elements, trans-factors as well as genomic features are known to influence their expression. In Arabidopsis, besides a predominant p...
Preprint
Full-text available
Beyond their key role in translation, cytosolic transfer RNAs (tRNAs) are involved in a wide range of other biological processes. Nuclear tRNA genes (tDNAs) are transcribed by the RNA polymerase III (RNAP III) and cis-elements, trans-factors as well as genomic features are known to influence their expression. In Arabidopsis, besides a predominant p...
Article
Full-text available
Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poor...
Preprint
Full-text available
Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poor...
Article
Full-text available
Land plants and other photosynthetic organisms (algae, bacteria) use the beneficial effect of sunlight as a source of energy for the photosynthesis and as a major source of information from the environment. However, the ultraviolet component of sunlight also produces several types of damage, which can affect cellular and integrity, interfering with...
Article
Full-text available
DNA methylation contributes to key regulatory processes during plant development by controlling gene expression. Genomic methylation patterns are dynamic and must be properly maintained and/or re-established upon DNA replication and active removal, therefore requiring sophisticated control mechanisms. Here, we identified a direct interplay between...
Article
Significance As obligate photosynthetic and sessile organisms, plants are particularly exposed to the damaging effects of excess light and UV wavelengths, which can impact genome integrity by inducing DNA sequence alterations. As a response, plants have evolved efficient genome surveillance processes, some of which appear to also overlap with mecha...
Article
Full-text available
In the expanding repertoire of small noncoding RNAs (ncRNAs), tRNA-derived RNA fragments (tRFs) have been identified in all domains of life. Their existence in plants has been already proven but no detailed analysis has been performed. Here, short tRFs of 19-26 nucleotides were retrieved from Arabidopsis thaliana small RNA libraries obtained from v...
Article
As obligate photosynthetic organisms plants are particularly exposed to the damaging effects of excess light and ultraviolet wavelengths, which can impact genome and epigenome dynamics by inducing DNA sequence and chromatin alterations. DNA DAMAGE-BINDING PROTEIN 2 (DDB2) is the main factor involved in the recognition of UV-induced DNA lesions duri...
Article
Full-text available
In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE-BINDING PROTEIN 2 (DDB2) and the DNA methylatio...
Article
Full-text available
Homologous recombination (HR) is essential for maintaining genome integrity and variability. To orchestrate HR in the context of chromatin is a challenge, both in terms of DNA accessibility and restoration of chromatin organization after DNA repair. Histone chaperones function in nucleosome assembly/disassembly and could play a role in HR. Here, we...
Article
Full-text available
Flowering at the right time is crucial to ensure successful plant reproduction and seed yield and is dependent on both environmental and endogenous parameters. Among the different pathways that impinge on flowering, the autonomous pathway promotes floral transition independently of day length through the repression of the central flowering represso...
Article
Full-text available
Protein ubiquitylation regulates a broad variety of biological processes in all eukaryotes. Recent work identified a novel class of cullin-containing ubiquitin ligases (E3s) composed of CUL4, DDB1, and one WD40 protein, believed to act as a substrate receptor. Strikingly, CUL4-based E3 ligases (CRL4s) have important functions at the chromatin level...
Article
Full-text available
Plants and many other eukaryotes can make use of two major pathways to cope with mutagenic effects of light, photoreactivation and nucleotide excision repair (NER). While photoreactivation allows direct repair by photolyase enzymes using light energy, NER requires a stepwise mechanism with several protein complexes acting at the levels of lesion de...
Article
Full-text available
Summary Obligate photoautotrophs such as plants must capture energy from sunlight and are therefore exposed to the damaging collateral effects of ultraviolet (UV) irradiation, especially on DNA. Here we investigated the interconnection between light signaling and DNA repair, two concomitant pathways during photomorphogenesis, the developmental tran...
Article
The transgenic plant performance depends on the stable expression of the integrated transgene. In this paper, we have analyzed the stability of the most frequently used constitutive promoter, the cauliflower mosaic virus (CaMV) 35S promoter. We used several independent Nicotiana tabacum lines transgenic for the luciferase (LUC) or green fluorescenc...
Article
Full-text available
Author Summary Recent research revealed strong links between Cullin4 (CUL4)–based cullin-RING ubiquitin ligases (CRLs) and chromatin biology, including DNA replication and DNA repair. During Nucleotide Excision Repair (NER), CUL4 together with DDB1 (DNA Damage Binding protein 1) ubiquitylate an increasingly large number of substrates, including com...
Article
Full-text available
The coding sequence of Δ9-stearoyl-(acyl carrier protein) desaturase from Ricinus communis was introduced into sunflower, under the control of seed-specific promoter and terminator sequences of the late embryogenesis abundant gene from sunflower, Hads10. Two independent primary transformants contained three and six copies of the T-DNA, as demonstra...
Data
UV-C sensitivity of DDB1A and DDB2 overexpressing plants and mutants sensitivities to Cisplatin and H2O2. (A) Root-growth assay. One-week-old DDB1A and DDB2 independent overexpressor lines and WT control plants were exposed to 900 J/m2 of UV-C. Root growth was measured 24h following irradiation. Eight independent plants were analysed per lines and...
Data
DxR-mutated DDB2 binds DDB1A in yeast. (A) Schematic representation of DDB2 carrying four WD40 domains. The DxR motif at the end of the third WD40 domain is indicated as well as the point mutation R343H used for the yeast two-hybrid assay. (B) Yeast two-hybrid interaction between DDB1A and DDB2 and its mutated version (R343H). No auto-activation by...
Data
Steady state of GFP-DDB2 protein levels and quantification of DDB2 protein levels upon various genotoxic stresses. (A) Immunoblot revealing GFP-DDB2 content of pOEX4GFP-DDB2 Arabidopsis transgenic plants upon exposure to UV-C (900 J/m2), Cisplatin (10 µM) and H2O2 (5 mM). Coomassie blue staining was used as loading control. (B) Immunoblot revealing...
Article
We analyzed the influence of acute and chronic ionizing radiation (IR) on plant genome stability and global genome expression. Plants from the "chronic" group were grown for 21 days on (137)Cs-artificially contaminated soil, and received a cumulative dose of 1Gy. The "acute" plant group was exposed to an equal dose of radiation delivered as a singl...
Article
Full-text available
Owing to their sessile nature, plants are constantly exposed to a multitude of environmental stresses to which they react with a battery of responses. The result is plant tolerance to conditions such as excessive or inadequate light, water, salt and temperature, and resistance to pathogens. Not only is plant physiology known to change under abiotic...
Article
Full-text available
CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) is a negative regulator of photomorphogenesis in Arabidopsis thaliana. COP1 functions as an E3 ubiquitin ligase, targeting select proteins for proteasomal degradation in plants as well as in mammals. Among its substrates is the basic domain/leucine zipper (bZIP) transcription factor ELONGATED HYPOCOTYL5 (HY5)...
Article
Full-text available
Arabidopsis thaliana CENTRIN2 (AtCEN2) has been shown to modulate Nucleotide Excision Repair (NER) and Homologous Recombination (HR). The present study provides evidence that AtCEN2 interacts with the Arabidopsis homolog of human XPC, AtRAD4 and that the distal EF-hand Ca(2+) binding domain is essential for this interaction. In addition, the synthe...
Article
Oligonucleotide microarray technology was used to identify genes, which are responding after exposure to UV-C radiation and to other agents causing genotoxic stress. The effect of these conditions on recombinational DNA repair was monitored in parallel. Global changes in gene expression were investigated in Arabidopsis wild-type plants challenged w...
Article
Homologous recombination creates covalent linkages between DNA in regions of highly similar or identical sequence. Recent results from several laboratories, many of them based on forward and reverse genetics in Arabidopsis, give insights into the mechanisms of the enzymatic machinery and the involvement of chromatin in somatic and meiotic DNA recom...
Article
Homologous recombination (HR) serves a dual role in providing genetic flexibility and in maintaining genome integrity. Little is known about the regulation of HR and other repair pathways in the context of chromatin. We report on a mutant affected in the expression of the Arabidopsis INO80 ortholog of the SWI/SNF ATPase family, which shows a reduct...
Article
Full-text available
Two different roles for SNM (sensitive to nitrogen mustard) proteins have already been described: the SNM1/PSO2-related proteins are involved in the repair of the interstrand crosslink (ICL) and the ARTEMIS proteins are involved in the V(D)J recombination process. Our study shows that an Arabidopsis SNM protein, although structurally closer to the...
Article
Full-text available
A genetic screen of a population of Arabidopsis thaliana lines exhibiting enhanced somatic homologous recombination yielded a mutant affected in expression of a gene encoding a caltractin-like protein (centrin). The hyperrecombinogenic phenotype could be reproduced using RNA interference (RNAi) technology. Both the original mutant and the RNAi plan...
Article
Full-text available
Intermolecular recombination events were monitored in Arabidopsis thaliana lines using specially designed recombination traps consisting of tandem disrupted beta-glucuronidase or luciferase reporter genes in direct repeat orientation. Recombination frequencies (RFs) varied between the different lines, indicating possible position effects influencin...
Article
Full-text available
Agrobacterium -mediated transformation of shoot apices of sunflower (Helianthus annuus L.) was evaluated following wounding by cell-wall-digesting enzymes and sonication. The frequency of explants with regenerated shoots expressing GUS (beta-glucuronidase) or GFP (green fluorescent protein) increased following treatment with the macerating enzymes...
Article
The limiting step in the transformation protocol for sunflower (Helianthus annuus L.) is the induction of adventitious shoots from embryonic axes. This specific element of an established protocol was addressed in the present study. The explants were bombarded with tungsten particles coated with a construct harbouring the ipt gene of Agrobacterium v...
Article
Immature zygotic embryos of sunflower (Helianthus annuus L.) produce somatic embryos when cultured on medium supplemented with a cytokinin as the sole source of exogenous growth regulators. The timing of the induction phase and subsequent morphogenic events have been well characterized in previous work. We address here the question of the role of e...
Chapter
Visible reporter genes are a very useful approach to evaluating the efficiency of newly developed protocols in plant transformation technology. The basic idea is to distinguish transformed regenerants (shoots or somatic embryos) in an heterogeneous population from untransformed ones without a reduction in the viability of the regeneration tissue, a...
Article
 Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results...
Chapter
Depending on the culture conditions, somatic embryos or adventitious shoots can be induced alternatively or simultaneously on immature zygotic embryos of sunflower (Helianthus annuus L.). Their relative number is function of the sucrose concentration of the medium. This culture system is well characterised: the induction of shoots or embryos can be...
Article
Full-text available
Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme -glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots...

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Project (1)
Project
We are interested in determining how and when chromatin-based or epigenetically controlled mechanisms influence plant adaptive responses to environmental cues, with a specific emphasis on light conditions.