Jean-Michel Boursiquot

Jean-Michel Boursiquot
L'institut Agro | Montpellier SupAgro | SUPAGRO · Institut des Hautes Etudes de la Vigne et du Vin

PhD

About

184
Publications
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Publications

Publications (184)
Article
Full-text available
The grape genus Vitis L. includes the domesticated V. vinifera, which is one of the most important fruit crop, and also close relatives recognized as valuable germplasm resources for improving cultivars. To resolve some standing problems in the species relationships within the Vitis genus we analyzed diversity in a set of 90 accessions comprising m...
Article
Full-text available
To cope with the challenges facing agriculture, speeding-up breeding programs is a worthy endeavor, especially for perennial species such as grapevine, but requires understanding the genetic architecture of target traits. To go beyond the mapping of quantitative trait loci (QTLs) in bi-parental crosses, we exploited a diversity panel of 279 Vitis v...
Article
Geographical distribution and diversity of current plant species have been strongly shaped by climatic oscillations during the Quaternary. Analyzing the resulting divergence among species and differentiation within species is crucial to understand the evolution of taxa like the Vitis genus, which provides very useful genetic resources for grapevine...
Preprint
Full-text available
Fanleaf virus disease, which was spread on a large scale during post-phylloxera reconstitution, represents today a major concern for most of vineyards worldwide. Without any efficient accessible mean of direct control at the present time, concerning an incurable disease whose nematods vectors are able to survive and remain infectious in the soil fo...
Preprint
Full-text available
To cope with the challenges facing agriculture, speeding-up breeding programs is a worthy endeavor, especially for perennials, but requires to understand the genetic architecture of important traits. To go beyond QTL mapping in bi-parental crosses, we exploited a diverse panel of 279 Vitis vinifera L. cultivars. This panel planted in five blocks in...
Article
Full-text available
Double-stranded RNAs and total RNAs purified from grapevine (Vitis vinifera) phloem scrapings of two varieties held in the INRAE (France) grapevine germplasm collection were analyzed by high-throughput sequencing. BLAST annotation revealed contigs with homology to Polerovirus genus members. The full genome sequence of one isolate (KT) was determine...
Article
To characterize their virome, double stranded RNAs extracted from scrapings of two Iranian grapevine varieties held in the Vassal-Montpellier Grapevine Biological Resources Center were analysed by high-throughput sequencing. In addition to several well-known grapevine viruses, divergent isolates of the newly described grapevine Kizil Sapak virus we...
Article
Abstract: Simón de Rojas Clemente y Rubio (1777-1827) is considered the father of modern ampelography. He developed the first scientific method for describing grapevine varieties through the examination of material he himself collected in Andalusia between 1802 and 1804. The Royal Botanical Garden in Madrid conserves the material he collected and p...
Article
Full-text available
Key message The maternal and paternal parentage of 36 rootstocks was determined and verified. The results of this study indicate that existing grape rootstocks are closely related to each other and have a narrow genetic background. Abstract Rootstocks are used to protect grapevines from biotic and abiotic stresses including phylloxera, nematodes,...
Article
Full-text available
RNASeq or double-stranded RNA based approaches allowed the reconstruction of a total of 9 full-length or near full-length genomes of the recently discovered grapevine virus T (GVT). In addition, datamining of publicly available grapevine RNASeq transcriptome data allowed the reconstruction of a further 14 GVT genomes from five grapevine sources. To...
Data
Sequences of the GVT isolates described in the present work. The sequences are additionally available in Genbank under the accession numbers provided in the text and tables. (TXT)
Data
Phylogenetic tree reconstructed using the amino acid sequence of the replication-associated protein (REP) of GVT and of GRSPV isolates. The Neighbor joining tree was reconstructed using a strict amino acid distance in Mega7. Bootstrap analysis (1,000 replicates) was performed to evaluate the solidity of branches. Only bootstrap values >70% are show...
Data
Phylogenetic tree reconstructed using the amino acid sequence of the coat protein (CP) proteins of GVT and of RSPV isolates. The Neighbor joining tree was reconstructed using a strict amino acid distance in Mega7. Bootstrap analysis (1,000 replicates) was performed to evaluate the solidity of branches. Only bootstrap values >70% are shown. The scal...
Article
Full-text available
The wine industry is facing critical issues due to climate changes since production is established on very tight Genotype × Environment interaction bases. While, some cultivation practices may reduce adverse effects of abiotic stresses on the vines, e.g., the use of irrigation to mitigate drought, the deleterious impacts of warming on fruit develop...
Article
Full-text available
Grapevine is a very important crop species that is mainly cultivated worldwide for fruits, wine and juice. Identification of the genetic bases of performance traits through association mapping studies requires a precise knowledge of the available diversity and how this diversity is structured and varies across the whole genome. An 18k SNP genotypin...
Article
Full-text available
It is briefly described the various introductions of Vitis vinifera L. in Chile and its expansion in the territory, as well as the predominance of the French paradigm in its more recent development. From the beginning of the 21st century, the first steps are taken in search of a new identity, with the introduction of varieties of different origins...
Article
Full-text available
Background and Aims: Based on 19 nuclear simple sequence repeat markers and parental analysis, we aimed to identify and propose the pedigree of different accessions held at the Estación Experimental Agropecuaria Mendoza of the Instituto Nacional de Tecnología Agropecuaria germplasmcollection. The results were compared with data recorded in large, i...
Article
Full-text available
The complete nucleotide sequence of an isolate of grapevine satellite virus (GV-Sat) was determined by next-generation sequencing (NGS) and compared with the single available complete sequence. The NGS data unexpectedly provided evidence for the existence of multimeric forms of GV-Sat, which were experimentally confirmed, allowing the redefinition...
Article
Full-text available
Abstract A.S. Gonzalez, F. Massera, D. Moscoso, P. Hinrichsen, G. Montenegro, V. Laucou, T. Lacombe, J.-M. Boursiquot, and Ph. Pszczólkowski. 2016. Identification and characterization of an original grapevine cultivar (Vitis vinifera) found in Chile. Cien. Inv. Agr. 43(2):21-31. Currently, many monovarietal vineyards present a minority of mixed cul...
Article
Full-text available
During the 2015 season, ICV Group consultants observed a "chlorophyll defect" (formerly known as "albinos") morphological variation in a parcel of Grenache N that is part of the "Ventoux Observatory" in the South of France. An anomaly of the same type was also reported in the Pyrénées-Orientales for Chardonnay B variety. These manifestations are mo...
Article
Full-text available
We propose here a decision support system for vine growers to assess the quality of a vineyard to be planted. The quality of a vineyard is defined by the probability of possible profitability of the wine sales he is able to produce. The model, based on a Bayesian network (BN), takes into account environment and the parameters defining vineyard stat...
Article
Full-text available
The study of accessions from Argentina may provide a valuable testimony on the origins of the different genetic resources and varieties which were sought and used to develop the vineyard of this country. 54 accessions introduced in the Vassal collection were analyzed using 20 microsatellite markers and ampelographic descriptors. Among them, 43 dist...
Article
Full-text available
A total of 90 grapevine samples collected in five countries of the Western Balkan region were evaluated for trueness-to-type and kinship relations based on comparative analysis with 1,130 grapevine genotypes held at the INRA "Domaine de Vassal" French Grape Germplasm Repository, using 14 microsatellite markers. In the context of the comparative ana...
Article
Full-text available
Background [br/] Interaction between TERMINAL FLOWER 1 (TFL1) and LEAFY (LFY) seem to determine the inflorescence architecture in Arabidopsis. In a parallel way, overexpression of VvTFL1A, a grapevine TFL1 homolog, causes delayed flowering and production of a ramose cluster in the reiterated reproductive meristem (RRM) somatic variant of cultivar C...
Article
Full-text available
Background Interaction between TERMINAL FLOWER 1 (TFL1) and LEAFY (LFY) seem to determine the inflorescence architecture in Arabidopsis. In a parallel way, overexpression of VvTFL1A, a grapevine TFL1 homolog, causes delayed flowering and production of a ramose cluster in the reiterated reproductive meristem (RRM) somatic variant of cultivar Carigna...
Article
Since 1997, many studies have been performed based on the inheritance of nuclear microsatellite markers to verify or uncover the parentage of several grapevine cultivars. The aim of the present study was to undertake an extended analysis using a large sample of Vitis vinifera cultivars held in the INRA "Domaine de Vassal" Grape Germplasm Repository...
Conference Paper
The current chromosome assembly of the grapevine reference genome is under improvement using two strategies. First several maps have been densified using SNP markers developed both from a classic Sanger re-sequencing of the parents and from the re-sequencing of a panel of diversity in the Vitaceae genome to develop a 18K genotyping chip (http://urg...
Article
Full-text available
Cultivated grapevines, Vitis vinifera subsp. sativa, evolved from their wild relative, V. vinifera subsp. sylvestris. They were domesticated in Central Asia in the absence of the powdery mildew fungus, Erysiphe necator, which is thought to have originated in North America. However, powdery mildew resistance has previously been discovered in two Cen...
Article
Full-text available
Afin de contribuer au developpement des connaissances sur lepatrimoine ampelographique et les ressources genetiques viticoles de la Grece, nous avons caracterise la vigne dite “de Pausanias” au niveau ampelographique et moleculaire. Cette vigne se trouve dans le centre du Peloponnese (village de Pagrati, Prefecture d’Achaia). Elle est denommee et r...
Article
Full-text available
Background Grapevine (Vitis vinifera subsp. vinifera) is one of the most important and ancient horticultural plants in the world. Domesticated about 8–10,000 years ago in the Eurasian region, grapevine evolved from its wild relative (V. vinifera subsp. sylvestris) into very diverse and heterozygous cultivated forms. In this work we study grapevine...
Data
The four steps of the graphic method of Evanno et al. (2005), allowing the estimation of the true number of ancestral groups K.
Data
Geographic and agro-technological characterization of the STRUCTURE subgroups.
Data
List of the 2096 genotypes (introduction and variety names, cultivar code) and their main characteristics (geographic origin, utility, sex, skin color, flesh color, berry flavor and seeds).
Data
Diversity indexes for the 20 microsatellite loci and the 2,096 cultivated grapevine genotypes.
Data
Full-text available
Full dendrogram of the 2096 cultivated grapevines, according to a clustering based on the 20 SSR marker data and the Ward method. The short name of the varieties was used (use the zoom option to read). Group color codes and numbers are given in the Additional file 1, Table S1, "codes" sheet.
Data
Comparison of the clustering between the STRUCTURE and the Ward methods.
Data
Geographic and agro-technological characterization of the Ward subgroups.
Article
Full-text available
Inheritance of nuclear microsatellite markers (nSSR) has been proved to be a powerful tool to verify or uncover the parentage of grapevine cultivars. The aim of the present study was to undertake an extended parentage analysis using a large sample of Vitis vinifera cultivars held in the INRA "Domaine de Vassal" Grape Germplasm Repository (France)....
Article
Full-text available
Through multiple vegetative propagation cycles, clones accumulate mutations in somatic cells that are at the origin of clonal phenotypic diversity in grape. Clonal diversity provided clones such as Cabernet-Sauvignon N°470, Chardonnay N° 548 and Pinot noir N° 777 which all produce wines of superior quality. The economic impact of clonal selection i...
Data
Validation of random distribution of aligned reads. Coefficient correlation between the number of aligned reads and the length of the chromosome was tested using Pearson's correlation (R2, P-value<0.05). (TIF)
Data
Analysis of reads obtained with 454 for each clone using FastQC software. Quality mean per base for each position of base in reads. Quality decreases with length of reads. (TIF)
Data
The trees in were built from sequence consensus for Cauliv-1 sequence in 5′LTR. (see Figure 6 in main text). LTR homology sequence trees were obtained using the ClustalW algorithm with 1000 permutations and the neighbor-joining method. (TIF)
Data
The trees in were built from sequence consensus for Gypsy-19 sequence in 5′LTR. (see Figure 6 in main text). LTR homology sequence trees were obtained using the ClustalW algorithm with 1000 permutations and the neighbor-joining method. (TIF)
Data
Results of S-SAP for 4 mobile elements analyzed in detail (Caul-1, Gret-1, Copia 10, Gypsy 19). Number of polymorphism bands detected between 2 clones generate by 4 mobile elements analyzed. (DOC)
Data
We detail in this section the alignment method and S-SAP protocol using in this study. (DOC)
Data
Percentage of aligned bases with different quality alignment scores. 90% of aligned bases had a quality score of more than 60. (TIF)
Data
The trees in were built from sequence consensus for Copia-10 sequence in 5′LTR. (see Figure 6 in main text). LTR homology sequence trees were obtained using the ClustalW algorithm with 1000 permutations and the neighbor-joining method. (TIF)
Data
The first list contains polymorphic mobile elements detected in our data set and ranked by increasing number of polymorphisms. The second list contains mobile elements detected in the sequenced genomes ranked by increasing number of mobile element copies. (DOC)
Data
Details of polymorphisms detected among clones (SNPs, In/Dels and Mobile elements) with a depth greater than 6× and a base alignment quality score of more than 60 for each of the 3 comparisons. (DOC)
Data
Polymorphisms located in genes between clones. Position corresponds to the beginning of the gene on the genome browser. (DOC)
Article
Full-text available
Estimates of climate conditions before the 19th century are based on proxy data reconstructions or sparse meteorological measurements. The reconstruction of the atmospheric circulation that prevailed during the European Little Ice Age (∼1500–1850) has fostered many efforts. This study illustrates a methodology, combining historical proxies and mode...
Article
Full-text available
The objective of the European Vitis Database is to safeguard and enhance germplasm by monitoring its preservation. Two issues are strongly related to that purpose: (1) participation of collections covering almost all grape biodiversity and (2) assessment of accessions trueness to type. In the scope of the European project GrapeGen06 efforts have be...
Article
Full-text available
Fiano is a white grapevine variety from southern Italy, in the Campania region around Naples. It produces the prestigious wine Fiano di Avellino and, as is the case with many old varieties in Europe, it has been known under a number of synonyms. There is a growing interest in Fiano in Australia, with some lovely complex and textural wines produced...
Book
The European Vitis Database (www.eu-vitis.de) - a technical innovation through an online uploading and interactive modification system
Article
Full-text available
Grape diversity present in Morocco and the part of this diversity used nowadays are poorly documented. In order to choose diversified genotypes, to select them so that their agronomic interest will be tested, a group of 21 autochthonous cultivars preserved in the germplasm collections of SODEA and 18 Moroccan cultivars from “Domaine de Vassal” INRA...
Article
Full-text available
Estimates of climate conditions before the 19th century are based on proxy data reconstructions or sparse meteorological measurements. The reconstruction of the atmospheric circulation that prevailed during the European Little Ice Age (~1500-1850) has fostered many efforts. This study illustrates a methodology combining historical proxies and moder...