Jean-Francois Sassi

Jean-Francois Sassi
Atomic Energy and Alternative Energies Commission | CEA · CEA Tech en Région Sud

Doctor of Philosophy

About

64
Publications
23,256
Reads
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1,983
Citations
Additional affiliations
March 2013 - present
Atomic Energy and Alternative Energies Commission
Position
  • Manager - 3rd Generation Biomass
March 2006 - March 2013
Centre d'Etude et de Valorisation des Algues (CEVA)
Position
  • Head of Department
January 2000 - February 2006
Rhodia
Position
  • Senior Researcher

Publications

Publications (64)
Article
Full-text available
Microalga-based fuels are promising solutions for replacing fossil fuels. This feedstock presents several advantages such as fast growth in a harsh environment and an ability to trap gases emitted from industries, thus reducing global warming effects. An efficient way to convert harvested microalgae into biofuels is hydrothermal liquefaction (HTL),...
Article
Full-text available
Hydroponic farms produce wastewater that need to be treated before being released into the environment. A three-step screening process (microplate, batch, and semi-continuous flasks experiments) initially designed to select an efficient microalgae strain allowed the isolation of a consortium that naturally developed in the hydroponic farm wastewate...
Article
Full-text available
Cyanobacteria, the largest phylum of prokaryotes, perform oxygenic photosynthesis and are regarded as the ancestors of the plant chloroplast and the purveyors of the oxygen and biomass that shaped the biosphere. Nowadays, cyanobacteria are attracting a growing interest in being able to use solar energy, H2O, CO2 and minerals to produce biotechnolog...
Article
Full-text available
Cyanobacteria constitute the only phylum of oxygen-evolving photosynthetic prokaryotes that shaped the oxygenic atmosphere of our planet. Over time, cyanobacteria have evolved as a widely diverse group of organisms that have colonized most aquatic and soil ecosystems of our planet and constitute a large proportion of the biomass that sustains the b...
Article
Full-text available
Microalgae produce a variety of compounds that are beneficial to human and animal health. Among these compounds are carotenoids, which are microalgal pigments with unique antioxidant and coloring properties. The objective of this review is to evaluate the potential of using microalgae as a commercial feedstock for carotenoid production. While micro...
Article
Microalgae were considered in this work as a new resource for developing starch-based bioplastics. Ten green microalgae strains were screened at lab-scale for their ability to produce starch. A long run (800 h) two-stage accumulation strategy was designed with successive cultivation in sulfur-replete, then sulfur-depleted medium in autotrophic cond...
Article
Most of our knowledge on the mechanisms underlying diatom‐bacterial interactions has been acquired through studies involving isolation of culturable partners. Here, we established a laboratory model of intermediate complexity between complex natural communities and laboratory pure culture models. We investigated the whole community formed by the fr...
Article
Full-text available
Arthrospira platensis (Spirulina) is the most cultivated microalga worldwide. Improving its cultivation in terms of biomass productivity, quality, or production cost could significantly impact the Spirulina industry. The objectives of this paper were defined as to contribute to this goal. Spirulina biomass productivity was investigated through medi...
Poster
Full-text available
Microalgae can be efficiently used to remove a wide spectrum of industrial pollutants. Taking into account the large amount of microalgae species and industrial pollutants, a high throughput methodology is therefore needed to explore all bioremediation possibilities. A flow cytometry high-throughput methodology has been developed in order to screen...
Article
Full-text available
Microalgae have been shown to be a source of multiple bio-based products ranging from high value molecules to commodities. Along with their potential to produce a large variety of products, microalgae can also be used for the depollution of wastewaters of different origins (urban,industrial, and agricultural). This paper is focused on the importanc...
Article
Coagulation–flocculation is an efficient, economic, and scalable process suitable for harvesting microalgae. The study tested the effectiveness of 11 agents (chitosan and 10 commercial, industrially available reference products) for harvesting Chlamydomonas reinhardtii, a model microalga. The highest efficiencies (> 95%) with the lowest dose (< 30...
Article
Full-text available
Sulphated polysaccharides (SP) extracted from seaweeds have antiviral properties and are much less cytotoxic than conventional drugs, but little is known about their mode of action. Combination antiviral chemotherapy may offer advantages over single agent therapy, increasing efficiency, potency and delaying the emergence of resistant virus. The par...
Patent
The present invention notably relates to ulvan lyases, to nucleic acid sequences coding for these ulvan lyases, to vectors comprising these coding sequences, to a method of manufacturing these ulvan lyases, as well as to a method of degrading ulvans using these ulvan lyases and applicable applications to the degradation products of the ulvans. The...
Article
Full-text available
This project aimed at assessing technical, economic and environmental relevance of a new substrate from marine herb (eelgrass, Zostera noltii) by comparing it to the products mainly used today in the field of vegetable soilless hydroponic cropping. It aimed in particular to take advantage of an endogenous asset original and specific of the eelgrass...
Article
Full-text available
Ulvans are cell wall matrix polysaccharides in green algae belonging to the genus Ulva. Enzymatic degradation of the polysaccharide by ulvan lyases leads to the production of oligosaccharides with an unsaturated β-glucuronyl residue located at the non-reducing end. Exploration of the genomic environment around the Nonlabens ulvanivorans (previously...
Article
Algae are a promising source of industrial biomass for the future. In order to assess if aquacultured seaweed (macroalgae) could be considered an environmentally friendly source of biomass for bioenergy, life cycle assessments were performed for European countries, comparing methane as a biofuel from the anaerobic digestion (A) of whole seaweeds, (...
Article
Polysaccharides are the most abundant and the most diverse renewable materials found on earth. Due to the stereochemical variability of carbohydrates, polysaccharide-degrading enzymes - i.e. glycoside hydrolases and polysaccharide lyases - are essential tools for resolving the structure of these complex macromolecules. The exponential increase of g...
Article
Full-text available
Ulvans are complex sulfated polysaccharides found in the cell walls of green algae belonging to the genus Ulva. These polysaccharides are composed of disaccharide repetition moieties made up of sulfated rhamnose linked to either glucuronic acid, iduronic acid, or xylose. Two ulvan lyases of 30 and 46 kDa were purified from the culture supernatant o...
Article
Seaweed (Ulva sp.) stranded on beaches were utilized as co-substrate for anaerobic digestion of pig slurry in three-month co-digestion tests in pilot scale anaerobic digesters in the laboratory. The methanogenic potential of Ulva sp. was low compared to that of other potential co-substrates available for use by farmers: 148 N m3CH4/t of volatile so...
Article
Full-text available
A rod shaped, Gram-stain-negative, chemo-organotrophic, heterotrophic, strictly aerobic, non-gliding bacterium, designated strain PLR(T), was isolated from faeces of the mollusc Aplysia punctata (Mollusca, Gastropoda) that had been fed with green algae belonging to the genus Ulva. The novel strain was able to degrade ulvan, a polysaccharide extract...
Article
The development of surfactants based on natural renewable resources is a concept that is gaining recognition in detergents, cosmetics, and green chemistry. This new class of biodegradable and biocompatible products is a response to the increasing consumer demand for products that are both “greener”, milder, and more efficient. In order to achieve t...
Article
The seasonal variability in the extraction yield, physicochemical characteristics, and rheological properties of ulvan from two Ulva species contributing to Brittany “green tides” has been studied. These seaweeds were collected in the water column for Ulva armoricana Dion, de Reviers et Coat and on hard substrata for Ulva rotundata Bliding. The max...
Article
Ultrastructural analysis of the gel forming green seaweed sulfated polysaccharide ulvan revealed a spherical-based morphology (10–18 nm diameter) more or less aggregated in aqueous solution. At pH 13 in TBAOH (tetrabutyl ammonium hydroxyde) or NaOH, ulvan formed an open gel-like structure or a continuous film by fusion or coalescence of bead-like s...
Article
Ulvan, the sulfated cell-wall polysaccharides from green seaweeds (Ulva species), presents structural and functional properties of interest for different applications. Its extraction yield in water varies depending on the species, the period of collect and the mode of conservation of algae. To identify limits of extraction, the structure and intera...
Article
Full-text available
A series of polymeric formulations has been prepared based on oxobiodegradable (PVA) and hydrobiodegradable (starch, PLA, Bionolle) macromolecular materials used as continuous matrices and fillers derived from a marine sea-weed (Zostera). The formulations have been submitted to processing trials under different experimental conditions selected in o...
Article
The impact of freezing, freeze-drying, hot-air drying, brining and dry salting stabilization methods of Ulvarotundata has been studied on the yield, physico-chemical characteristics and rheological properties of ulvan. Frozen (−30 and −80 °C) and freeze-dried algae yielded ulvan extracts mainly composed of high molecular weight polysaccharides. The...
Article
Full-text available
The water-soluble cell wall polysaccharides from green seaweeds of Ulva spp. (Ulvales, Chlorophyta), referred to as ulvan, demonstrate composition- and structure-related functional properties. Mid-infrared spectroscopy combined with chemometric techniques was investigated as a means to rapidly predict the chemical composition of ulvan extracts. A c...
Article
A straightforward grafting of a polycationic phosphorus-containing dendritic shell onto polystyrene nanoparticles leads to dendronized nanoparticles showing unique behavior.
Article
Full-text available
The relative reactivity of the I-alpha and I-beta phases of Valonia cellulose toward partial homogeneous acetylation was investigated by FT-IR and CP/MAS 13C-NMR spectroscopy. At the beginning of the acetylation and when only partial reaction was achieved, it was found that the reactivity of the I-alpha phase was substantially higher than that of t...
Article
Solution and interfacial properties of water-soluble hybrid linear-dendritic polyether copolymers are investigated by static and dynamic surface tension measurements and adsorption experiments on polymeric substrates. The results obtained show that the block copolymers are able to form mono-and multimolecular aggregates in water. Contacting a solid...
Article
An ultrastructural study of the acetylation of cellulose was achieved by subjecting well characterized cellulose samples fromValonia cell wall and tunicin tests to homogeneous and heterogeneous acetylation. The study involved transmission electron microscopy observations on negatively stained microcrystals as well as diffraction contrast images of...

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Projects (7)