Jean Colcombet

Jean Colcombet
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Plant Biology and Breeding

PhD, HDR, BTSA-GF

About

81
Publications
13,012
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3,564
Citations
Additional affiliations
January 2013 - December 2014
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Senior Researcher
January 2006 - December 2013
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Researcher
October 2005 - December 2006

Publications

Publications (81)
Article
Arabidopsis thaliana Mitogen-Activated Protein Kinases 3 and 6 (MPK3/6) are transiently activated during PAMP-Triggered Immunity (PTI) and durably during Effector-Triggered Immunity (ETI). However the functional differences between these two kinds of activation kinetics and how they coordinate the two layers of plant immunity remain poorly understo...
Article
Root endophytes establish beneficial interactions with plants, improving holobiont resilience and fitness, but how plant immunity accommodates beneficial microbes is poorly understood. The multi‐stress tolerance‐inducing endophyte Enterobacter sp. SA187 triggers a canonical immune response in Arabidopsis only at high bacterial dosage (>108 CFUs ml−...
Preprint
Arabidopsis thaliana Mitogen Activated Protein Kinases 3 and 6 (MPK3/6) are known to be activated transiently in PAMP-Triggered Immunity (PTI) and durably in Effector-Triggered Immunity (ETI). However the functional differences between these two kinds of activation kinetics and how they allow coordination of the two layers of plant immunity remain...
Article
Full-text available
In plants, Mitogen-Activated Protein Kinases (MAPKs) are important signaling components involved in developemental processes as well as in responses to biotic and abiotic stresses. In this review, we focus on the roles of MAPKs in Effector-Triggered Immunity (ETI), a specific layer of plant defense responses dependent on the recognition of pathogen...
Article
Abiotic and biotic factors cause plant wounding and trigger complex short- and long-term responses at the local and systemic levels. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of clade-A Mitogen Activated Protein Kinases (MAPKs) MPK3 and MPK6 by wou...
Preprint
Full-text available
Wounding is caused by abiotic and biotic factors and triggers complex short- and long-term responses at the local and systemic level. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of MKK4/5-MPK3/6 by wounding is independent of jasmonic acid (JA) signal...
Article
To perceive pathogens, plants employ pattern recognition receptor (PRR) complexes, which then transmit these signals via the receptor-like cytoplasmic kinase BIK1 to induce defense responses. How BIK1 activity and stability are controlled is still not completely understood. Here, we show that the Hippo/STE20 homolog MAP4K4 regulates BIK1-mediated i...
Article
Full-text available
Phospholipase D alpha 1 (PLDα1, AT3G15730) and mitogen-activated protein kinases (MAPKs) participate on signaling-dependent events in plants. MAPKs are able to phosphorylate a wide range of substrates putatively including PLDs. Here we have focused on functional regulations of PLDα1 by interactions with MAPKs, their co-localization and impact on sa...
Article
Full-text available
Mitogen activated protein kinase (MAPK) cascades play an important role in many aspects of plant growth, development, and environmental response. Because of their central role in many important processes, MAPKs have been extensively studied using biochemical and genetic approaches. This work has allowed for the identification of the MAPK genes and...
Article
In plant-microbe interactions, a pathogenic microbe initially has to overcome preformed and subsequently induced plant defenses. One of the initial host-induced defense responses is microbe-associated molecular pattern (MAMP)-triggered immunity (MTI). Successful pathogens attenuate MTI by delivering various effectors that result in effector-trigger...
Data
PLDα1-YFP localization in trichoblast (labeled as T) and atrichoblast (labeled as A) rhizodermis cell files of the root tip of rescued pldα1-2 mutant stably transformed with proPLDα1::PLDα1:YFP construct by light-sheet fluorescence microscopy. Localization of PLDα1-YFP, propidium iodide and merged image of the root transition zone in longitudinal (...
Data
3-D rendering of leaf epidermal petiole cell at the pre-prophase stage of cell division with established PPB and localization of PLDα1-YFP.
Data
PLDα1-YFP localization in different tissues of the root tip of rescued pldα1-1 mutant stably transformed with proPLDα1::PLDα1:YFP construct by light-sheet fluorescence microscopy. (A) Localization of PLDα1-YFP in cells of the root tip counterstained by propidium iodide and merged image. Profiles at different positions of the root tip are visualized...
Data
PLDα1-YFP localization in different tissues of the root tip of rescued pldα1-2 mutant stably transformed with proPLDα1::PLDα1:YFP construct by light-sheet fluorescence microscopy. (A) Localization of PLDα1-YFP in cells of the root tip counterstained by propidium iodide and merged image. Profiles at different positions of the root tip are visualized...
Data
3-D rendering of early disk phragmoplast in root meristematic cell at the cytokinesis with localization of PLDα1-YFP.
Data
Immunofluorescence localization of PLDα1 protein in Arabidopsis root meristem cells of wild type Col-0 seedlings showing homogeneous distribution of PLDα1 in the cytoplasm.
Data
Organization of microtubule arrays in dividing cells of root meristem in pldα1-1 mutant in comparison to wild type Col-0. Arrowheads indicate PPBs, red arrows mitotic spindles and white arrows phragmoplasts. Immunofluorescence localization of microtubules with confocal microscopy, nuclei are counterstained with DAPI.
Data
3-D rendering of late ring phragmoplast in root meristematic cell at the cytokinesis with localization of PLDα1-YFP.
Data
Comparison of PLDα1-YFP localization in different aerial organs and tissues of pldα1-1 (A–E) and pldα1-2 (F–J) mutants stably expressing PLDα1-YFP driven by its own promoter: epidermis of first true leaf (A,F), epidermal cells of hypocotyl (B,G), epidermal cells of cotyledon petiole (C,H), epidermal cells of cotyledon (D,I) and entire 5 days-old se...
Data
PLDα1-YFP localization in trichoblast (labeled as T) and atrichoblast (labeled as A) rhizodermis cell files of the root tip of rescued pldα1-1 mutant stably transformed with proPLDα1::PLDα1:YFP construct by light-sheet fluorescence microscopy. Localization of PLDα1-YFP, propidium iodide and merged image of the root transition zone in longitudinal (...
Data
Immunofluorescence colocalization of microtubules with PLDα1–YFP and clathrin in Arabidopsis root cells of complemented pldα1-1 mutant expressing PLDα1–YFP. (A) Colocalization of microtubules (green), PLDα1–YFP (red), and clathrin (blue) in late phragmoplast of root meristematic cell during the cytokinesis. (B) Colocalization of cortical microtubul...
Data
3-D rendering of leaf epidermal petiole cell at the cytokinesis with ring phragmoplast and localization of PLDα1-YFP.
Article
Full-text available
Author summary Plants as sessile organisms are facing multiple stresses during their lifetime. Among them, abiotic stresses, such as salt stress, can cause severe crop yield reduction, leading to food security issues in many regions of the world. In order to respond to growing food demands, especially in the context of the global climate change and...
Data
RNAseq analysis. Whole genome analysis based on TAIR10 annotation. For each comparison, log2 fold change is indicated as well as adjusted p-value (q-value). (XLSX)
Data
GO term analysis. GO term significant enrichment for every cluster. Gene enrichment analyses were performed using AmiGO1 website (http://amigo1.geneontology.org/cgi-bin/amigo/term_enrichment), using default parameter. (XLSX)
Data
Meteorological data for field trials in Hada Al-Sham. Precipitations and maximal/minimal temperature recorded in experimental agriculture facility in Hada Al-Sham where field trials with alfalfa were conducted in seasons 2015–16 and 2016–17. (PDF)
Data
Quantification of root colonization by SA187. Efficiency of root colonization evaluated by counting colony forming units (CFU) and normalized per root centimeter. Seedlings were grown on ½ MS medium (Control) or ½ MS with 100 mM NaCl for 5 days. Bars represent SE, n = 9, each sample consists of 5 roots. Asterisks indicate a statistical difference b...
Data
Growth of SA187-treated Arabidopsis mutants in hormonal pathways under normal conditions. Fresh weight (mg) of SA187-colonized plants after growth on ½ MS for 17 days. All plots represent the mean of three biological replicates (n > 36). Error bars represent SE. + acs represents the heptuple mutant acs1-1 acs2-1 acs4-1 acs5-2 acs6-1 acs7-1 acs9-1,...
Data
The effect of ACC and KMBA treatment on Arabidopsis growth in comparison to SA187-inoculated seedlings. Complete data to spider graphs in Figs 7C and 8B. Fresh weight (A), root length (B), and lateral root density (C) of 17-day-old seedlings grown on ½ MS + 100 mM NaCl for the last 12 days. Values represent means of three biological experiments, ea...
Data
The effect of inactivated and GFP-tagged SA187 on Arabidopsis growth. (A) Fresh weight of 17-day-old Arabidopsis seedlings exposed to salt stress (½ MS + 100mM NaCl) for 12 days in the presence of heat-inactivated SA187 in comparison to living SA187. (B) Fresh weight of 17-day-old Arabidopsis seedlings exposed to salt stress (½ MS + 100mM NaCl) for...
Data
Primers used during this study. (PPTX)
Data
Scheme of SA187 inoculation and plant treatments. Sterilized seeds were placed on agar plates containing either ½ MS or ½ MS + SA187 (2·105 cells/ml), defining the mock- and SA187-inoculated plants, respectively. Five days after germination, mock and SA187-inoculated were transferred on control agar plates (½ MS) or stress agar plates (containing 1...
Data
Root hair length of 16-day-old seedlings. Average root hair length of 10% longest root hairs (n > 100) in 16-day-old seedlings grown vertically on ½ MS medium with or 100 mM NaCl. Seedlings were transferred 5 days after germination from ½ MS agar plates without (mock) or with SA187. Only root hairs emerged after the seedling transfer were measured....
Data
Ethylene emission by SA187 on synthetic medium. (A) Ethylene emission of in vitro SA187 cultures at different stages after inoculation. Average OD600 values at each time point are given. Grey bar: LB medium without SA187; Green bars: LB medium with SA187. (B) Ethylene emission corrected for background ethylene levels emitted by controls and standar...
Article
Full-text available
Phospholipase D alpha 1 (PLDα1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular and physiological processes, such as cytoskeletal remodeling, regulation of stomatal closure and opening, as well as biotic and abiotic stress signaling. Here we aimed to study developmental expression patterns and subcellular loc...
Book
In plant-microbe interactions, a pathogenic microbe initially has to overcome preformed and subsequently induced plant defenses. One of the initial host-induced defense responses is microbe-associated molecular pattern (MAMP)-triggered immunity (MTI). Successful pathogens attenuate MTI by delivering various effectors that result in effector-trigger...
Article
Mitogen Activated Protein Kinases (MAPKs) are known to be important mediators of plant responses to biotic and abiotic stresses. In a recent report, we enlarged the understanding of the Arabidopsis thaliana MPK3 functions showing that the expression of a constitutively active (CA) form of the protein led to auto-immune phenotypes. CA-MPK3 plants ar...
Article
Full-text available
Background Microbial-associated molecular patterns activate several MAP kinases, which are major regulators of the innate immune response in Arabidopsis thaliana that induce large-scale changes in gene expression. Here, we determine whether microbial-associated molecular pattern-triggered gene expression involves modifications at the chromatin leve...
Article
Mitogen-Activated Protein Kinase (MAPK) cascades are functional modules widespread among eukaryotic organisms. In plants, these modules are encoded by large multigenic families and are involved in many biological processes ranging from stress responses to cellular differentiation and organ development. Furthermore, MAPK pathways are involved in the...
Article
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwa...
Article
Full-text available
Since its first description in 1995 and functional characterization 12 years later, plant MKK3-type MAP2Ks have emerged as important integrators in plant signaling. Although they have received less attention than the canonical stress-activated mitogen-activated protein kinases (MAPKs), several recent publications shed light on their important roles...
Article
To respond to abiotic stresses, plants have developed specific mechanisms that allow them to rapidly perceive and respond to environmental changes. The phytohormone abscisic acid (ABA) was shown to be a pivotal regulator of abiotic stress responses in plants, triggering major changes in plant physiology. The ABA core signaling pathway largely relie...
Article
Full-text available
Abscisic acid (ABA) is a major phytohormone mediating important stress-related processes. We recently unveiled an ABA-activated MAPK signaling module constituted of MAP3K17/18-MKK3-MPK1/2/7/14. Unlike classical rapid MAPK activation, we showed that the activation of the new MAPK module is delayed and relies on the MAP3K protein synthesis. In this a...
Article
Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative MAPK targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo...
Article
In nature, plants constantly have to face pathogen attacks. However, plant disease rarely occurs due to efficient immune systems possessed by the host plants. Pathogens are perceived by two different recognition systems that initiate the so-called pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), both of which are accompanied...
Article
Full-text available
Salmonella is one of the most prominent causes of food poisoning and growing evidence indicates that contaminated fruits and vegetables are an increasing concern for human health. Successful infection demands the suppression of the host immune system, which is often achieved via injection of bacterial effector proteins into host cells. In this repo...
Article
Full-text available
Background Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis, perception of microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 and MPK6. Increasing information depicts the molecular events activated by MAMPs in plants, but the specific and cooperative con...
Data
Mass spectrometric analyses of PIN cytoplasmic loop phosphorylation by D6PK. List of peptides including the phosphorylation state (PO3H2) of the respectively modified amino acid after phosphorylation of the recombinant PIN1–PIN4 cytoplasmic loops (CL) by D6PK. For each peptide identified from the PIN CLs, mass, charge state, and SEQUEST scores (cro...
Data
(A) Primer sequences used in this study. (B) Sequences of peptides used for phosphorylation experiments. (C) Peptides used for SRM analyses. DOI: http://dx.doi.org/10.7554/eLife.02860.033
Article
MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed...
Article
Proteins are major elements participating in all the key functions of the cells. They rarely fulfill their physiological roles in an autonomous way but rather act as part of more complex cellular machines. Indeed they can bind different types of molecules (proteins, nucleic acids, metabolites, etc.), via stable or transient interactions, depending...
Article
The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemin...
Book
MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed...
Article
Full-text available
Four hundred and fifty-eight genes coding for PentatricoPeptide Repeat (PPR) proteins are annotated in the Arabidopsis thaliana genome. Over the past 10 years, numerous reports have shown that many of these proteins function in organelles to target specific transcripts and are involved in post-transcriptional regulation. Therefore, they are thought...
Article
Mitogen-Activated Protein Kinase (MAPK) modules are often involved in stress responses and plant developmental processes. Among these MAPKs, MPK4 has a complex role in biotic stress signaling, cell division control and cytoskeletal organization. mpk4 knockout (KO) plants are dwarfed and very sick, making it difficult to distinguish between cause an...
Article
Mitogen-Activated Protein Kinase (MAPK) modules are often involved in stress responses and plant developmental processes. Among these MAPKs, MPK4 has a complex role in biotic stress signaling, cell division control and cytoskeletal organization. mpk4 knockout (KO) plants are dwarfed and very sick, making it difficult to distinguish between cause an...
Article
Full-text available
Plant mitogen-activated protein kinases (MAPKs) are involved in important processes, including stress signaling and development. In a functional yeast screen, we identified mutations that render Arabidopsis thaliana MAPKs constitutively active (CA). Importantly, CA-MAPKs maintain their specificity toward known activators and substrates. As a proof-...
Article
Full-text available
Plant genomes code for channels involved in the transport of cations, anions and uncharged molecules through membranes. Although the molecular identity of channels for cations and uncharged molecules has progressed rapidly in the recent years, the molecular identity of anion channels has lagged behind. Electrophysiological studies have identified S...
Article
Full-text available
Cytokinesis in plants is achieved by the formation of the cell plate. A pathway that includes mitogen-activated protein (MAP) kinase kinase kinase and MAP kinase kinase (MAPKK) plays a key role in the control of plant cytokinesis. We show here that a MAP kinase, MPK4, is required for the formation of the cell plate in Arabidopsis thaliana. Single m...
Article
Full-text available
Plants are constantly exposed to environmental biotic and abiotic stresses. Plants cells perceive these factors and trigger early responses followed by delayed and complex adaptation processes. Using cell suspensions of Arabidopsis thaliana (L.) as a cellular model, we investigated the role of plasma membrane anion channels in Reactive Oxygen Speci...
Article
Full-text available
Many changes in environmental conditions and hormones are mediated by MAPK (mitogen-activated protein kinase) cascades in all eukaryotes, including plants. Studies of MAPK pathways in genetic model organisms are especially informative in revealing the molecular mechanisms by means of which MAPK cascades are controlled and modulate cellular processe...
Article
Full-text available
Among the >200 members of the leucine-rich repeat receptor kinase family in Arabidopsis thaliana, only a few have been functionally characterized. Here, we report a critical function in anther development for the SOMATIC EMBRYOGENESIS RECEPTOR KINASE1 (SERK1) and SERK2 genes. B