Jean-Claude Sigoillot

Aix-Marseille Université | AMU · Ecole Supérieure d'Ingénieurs de Luminy

Sunflower and rapeseed meals are agro-industrial coproducts that contain high amount of phenolics (1-4 % dry matter), mostly as esters of caffeic acid (CA) and sinapic acid (SA), respectively. The enzymatic hydrolysis of the ester bonds enables to recover the corresponding free phenolic acids that are bioactive compounds and platform molecules for...

Background Lavender (Lavandula angustifolia) and lavandin (a sterile hybrid of L. angustifolia × L. latifolia) essential oils are among those most commonly used in the world for various industrial purposes, including perfumes, pharmaceuticals and cosmetics. The solid residues from aromatic plant distillation such as lavender- and lavandin-distilled...

The purpose of this work was to optimize the pretreatment process of wheat straw by Polyporus brumalis_BRFM985 in order to improve carbohydrate accessibility for more efficient bioconversion. Indeed, there is growing demands to develop sustainable routes for lignocellulosic feedstocks valorization into value-added products in energy, chemicals, mat...

Rapeseed meal is a cheap and abundant raw material, particularly rich in phenolic compounds of biotechnological interest. In this study, we developed a two-step bioconversion process of naturally occurring sinapic acid (4-hydroxy-3,5-dimethoxycinnamic acid) from rapeseed meal into canolol by combining the complementary potentialities of two filamen...

The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temp...

Implementation of cheap and eco-friendly biomass pretreatment processes is necessary to develop sustainable biorefineries. In nature, white-rot basidiomycetes are able to degrade lignin efficiently and selectively and are thus of great interest in such bioprocesses. In this study, five basidiomycetes strains were evaluated for their ability to pret...

Les champignons filamenteux des Phylum Ascomycètes et Basidiomycètes offrent un vaste répertoire de gènes codant pour une grande combinaison de mécanismes enzymatiques impliqués dans la dégradation de la biomasse lignocellulosique. La collection du CIRM-CF (Centre International de Ressources Microbiennes { Champignons Filamenteux, plateforme AMU-IN...

Second generation bioethanol, i.e., ethanol from lignocellulosic biomass is envisaged as a renewable source of transport fuel in the next years to complement or replace the first generation and the fossil fuel. At present, the most economic solution seems to be the enzymatic way that produces fermentable sugars from cellulose and hemicellulose. To...

At the beginning of 2016, first generation bioethanol still contributes to the majority of the 25 billion of gallons’ bioethanol produced worldwide, with the United States and Brazil producing approximately 85 % of the global production predominantly based on corn and sugarcane, respectively. However, concerns over the long-term sustainability of f...

The potential of fungal pretreatment to improve fermentable sugar yields from wheat straw or Miscanthus was investigated. We assessed 63 fungal strains including 53 white-rot and 10 brown-rot fungi belonging to the Basidiomycota phylum in an original 12 day small-scale solid-state fermentation (SSF) experiment using 24-well plates. This method offe...

Table S1. Fungal strains from the CIRM collection screened in this study.

Nowadays, it is essential to restructure various conventional industrial sectors (e.g. food, cosmetic, additive, pulp and paper industries, and bioethanol and biodiesel generation processes) into integrated biorefineries through valorisation of by-products streams. Rapeseed and sunflower are two of the most important oilseed crops in the world, aft...

Lactic acid is the building block of poly-lactic acid (PLA), a biopolymer that could be set to replace petroleum-based plastics. To make lactic acid production cost-effective, the production process should be carried out at low pH, in low-nutrient media, and with a low-cost carbon source. Yeasts have been engineered to produce high levels of lactic...

The Lavandula genus, which includes lavender (Lavandula angustifolia) and lavandin (L. angustifolia × Lavandula latifolia), is cultivated worldwide for its essential oils, which find applications in perfumes, cosmetics, food processing and, more recently, in aromatherapy products. The chemical composition of lavender and lavandin essential oils, us...

Filamentous fungi are well known for their ability to degrade lignocellulosic biomass and have a natural ability to convert certain products of biomass degradation, for example glucose, into various organic acids. Organic acids are suggested to give a competitive advantage to filamentous fungi over other organisms by decreasing the ambient pH. They...

Cinq souches fongiques ont été mises en œuvre dans un procédé de fermentation en milieu solide (FMS) afin de réaliser un prétraitement biologique de la paille de blé. Le traitement par FMS a été réalisé à 28°C pendant 21 jours. L’étude de la composition chimique de la paille de blé prétraitée a permis de mettre en évidence une délignification effic...

Accessible from: http://www.sciencedirect.com/science/article/pii/S2215016114000156 Lactic acid is a valuable and fully degradable organic acid with promising applications in Poly-lactic acid production. Despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application. To ameliorate lactic acid pr...

Récemment, un regain d’intérêt est porté à l’utilisation de résidus ligneux pour la production d’énergies renouvelables. Cela présente divers avantages mais également un défi technologique, de par la difficulté à hydrolyser la lignine contenue dans les végétaux. Ainsi, des prétraitements sont nécessaires. Le faible coût d’un prétraitement par des c...

Background: Saprophytic filamentous fungi are ubiquitous micro-organisms that play an essential role in photosynthetic carbon recycling. The wood-decayer Pycnoporus cinnabarinus is a model fungus for the study of plant cell wall decomposition and is used for a number of applications in green and white biotechnology. Results: The 33.6 megabase ge...

A number of microorganisms, bacteria and filamentous fungi, are able to degrade lignocellulosic components to various extents. However, only a few ones can degrade lignins, among which wood-rotting fungi. White-rot fungi, the most frequent ones, mineralize cell wall components (cellulose, hemicelluloses and lignins) and extensively degrade lignins,...

The genome of the coprophilic ascomycete Podospora anserina encodes 33 different genes encoding copper-dependent lytic polysaccharide monooxygenases (LPMOs) from glycoside hydrolase family 61 (GH61). In this study, two of these enzymes (P. anserina GH61A [PaGH61A] and PaGH61B), which both harbored a family 1 carbohydrate binding module, were succes...

Pycnoporus cinnabarinus laccase and a chimeric laccase-CBM were applied in softwood kraft pulp biobleaching in the presence of 1-hydroxybenzotriazole (HBT). The presence of CBM could enhance the laccase biobleaching potential as a decrease in the enzymatic charge and chlorine dioxide consumption, as well as an increase in pulp brightness were obser...

Rapeseed and sunflower are two of the world's major oilseeds. Rapeseed and sunflower meal (RSM and SFM), the by-products of oil extraction, are produced in large quantities. They are mainly composed of proteins, lignocellulosic fibres and minerals. They were initially used as a protein complement in animal feed rations and sometimes as fertilizer o...

Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. First, P. cinnabarinus growth conditions were optimized for...

The genus Pycnoporus forms a cosmopolitan group of four species belonging to the polyporoid white-rot fungi, the most representative group of homobasidiomycetes causing wood decay. Pycnoporus fungi are listed as food- and cosmetic-grade microorganisms and emerged in the early 1990s as a genus whose biochemistry, biodegradation and biotechnological...

Bacterial communities that can degrade surfactants have been selected from coastal seawaters contaminated by urban sewages. Only the linear fraction of commercial anionic surfactants was quickly degraded, and a residual fraction representing 10% of the initial concentration always remained. The highest concentrations of surfactant tolerated by thes...

In evergreen oak leaves collected at a western Mediterranean site (Var, France), the amounts of both hydrolyzable and condensed tannins decrease through the depth of the leaf litter. The decrease in condensed tannins could be partly explained by the cellular degradation of the lower epidermis and the mesophyll of leaves, as observed in histological...

Endo beta-1,4-mannanases (beta-mannanases, EC 3.2.1.78), belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA promoter and TtrpC terminator....

Paper production requires fibres to be refined, meaning mechanically treated to present sufficient bonding potential. As it is a highly energy consuming stage, various charges of cellulase as a pre-treatment were investigated to reduce the energy consumption and improve paper properties. The enzyme was added during pulp slushing and conditions of t...

The effect of three different commercial cellulase treatments applied on softwood bleached kraft pulp before the refining step was studied. Disc refining was applied under similar conditions as usual in paper mills. Two cellulase treatments allowed an energy saving of 20% to reach a given drainage index or breaking length. Water retention value (WR...

Laccase–mediator systems have the disadvantage that the mediator is expensive and potentially toxic. In this work, we used N-hydroxyacetanilide (NHA) in combination with laccase for the first time to bleach eucalypt pulp and found it to be a very promising, advantageous alternative to 1-hydroxybenzotriazole (HBT) as mediator. Thus, NHA is efficient...

Complete amino acid sequence of A. niger BK01 mannan endo-1,4-β mannosidase including native signal peptide. Amino acid sequence in FASTA format of the entire mannan endo-1,4-β-mannosidase from Aspergillus niger BK01, including putative signal peptide.

Mannans are key components of lignocellulose present in the hemicellulosic fraction of plant primary cell walls. Mannan endo-1,4-beta-mannosidases (1,4-beta-D-mannanases) catalyze the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of beta-mannans. Biodegradation of beta-mannans by the action of thermostable mannan endo-1,4-beta...

Pycnoporus cinnabarinus laccase was fused to the C-terminal linker and carbohydrate binding module (CBM) of Aspergillus niger cellobiohydrolase B (CBHB). The chimeric enzyme of molecular mass 100 kDa was successfully produced in A. niger. Laccase-CBM was further purified to determine its main biochemical properties. The Michaelis-Menten constant an...

Background Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and their proteinaceous components...

Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electroph...

Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electroph...

Poplar chips were treated by two white-rot fungi (Phanerochaete chrysosporium and Tranetes versicolor) separately, and then they were pulped by two P-RC APMP sequences (dosages of H2O2 3% and 6% respectively). Pulping energy consumption, pulp strength properties and optical properties were tested. The result showed that after the white-rot fungi tr...

Laccase production by the strain Pycnoporus cinnabarinus ss3 was studied in a solid-state culture on sugar-cane bagasse using chemical compounds as inducers (ethanol, methanol, veratryl alcohol and ferulic acid). Laccase productions were about 5- to 8.5-fold higher than non-induced cultures.Liquid-culture experiments with 14C-labeled ethanol were c...

The study on treatment of waste water of straw alkali pulp by white rot fungi has been made systemically in laboratory, the optimization conditions of white rot fungi culture have been given, and the relevant pilot-scale equipment of white-rot fungi treatment has been setup first in China. A reactor was used, using stainless steel wire as carrier f...

Agro-industrial by-products are a potential source of added-value phenolic acids with promising applications in the food and pharmaceutical industries. Here two purified feruloyl esterases from Aspergillus niger, FAEA and FAEB were tested for their ability to release phenolic acids such as caffeic acid, p-coumaric acid and ferulic acid from coffee...

The focus of this study was to improve conditions of use of fungal lignocellulolytic enzymes for conversion of lignocellulosic biomass to fermentable sugars for the production of bioethanol.Wheat straw was pre-treated by acid treatment with diluted sulfuric acid followed by steam explosion. Several enzymatic treatments implementing hydrolases (cell...

Wheat and oilseed flax straws were studied as raw material for papermaking. Two different aspects were investigated to valorize these agricultural byproducts: the capacity to recover some phenolic compounds and the use of the resulting cellulose fibers in papermaking. Straw phenolic compound composition was analyzed to determine the different acces...

We propose a new process using a vapor phase bioreactor (VPB) to simultaneously (i) delignify sugar-cane bagasse, a residue of sugar production that can be recycled in paper industry, and (ii) produce laccase, an enzyme usable to bleach paper pulp. Ethanol vapor, used as laccase inducer, was blown up through a VPB packed with bagasse and inoculated...

A new tyrosinase-encoding gene (2,204 bp) and the corresponding cDNA (1,857 nucleotides) from the white-rot fungus Pycnoporus sanguineus BRFM49 were cloned. This gene consisted of seven exons and six introns and encoded a predicted protein of 68 kDa, exceeding the mature tyrosinase by 23 kDa. P. sanguineus tyrosinase cDNA was over-expressed in Aspe...

Tyrosinases are type-3 copper proteins involved in the initial step of melanin synthesis. These enzymes catalyse both the o-hydroxylation of monophenols and the subsequent oxidation of the resulting o-diphenols into reactive o-quinones, which evolve spontaneously to produce intermediates, which associate in dark brown pigments. In fungi, tyrosinase...

The influence of gaseous ethanol addition as inducer of laccase produced by Pycnoporus cinnabarinus ss3 in a biofilter packed with sugar-cane bagasse was investigated. Gaseous-ethanol concentration up to 13.3 g m−3 allowed an important increment of laccase activity. Maximum of laccase production of 90 U g−1 dry support, which was 45 times higher th...

Wild and recombinant hydrolases and oxidoreductases with a potential interest for environmentally sound bleaching of high-quality paper pulp (from flax) were incorporated into a totally chlorine free (TCF) sequence that also included a peroxide stage. The ability of feruloyl esterase (from Aspergillus niger) and Mn2+-oxidizing peroxidases (from Pha...

Tyrosinase production by Pycnoporus cinnabarinus and Pycnoporus sanguineus was screened among 20 strains originating from various geographical areas, particularly from tropical environments. The tyrosinase from the most efficient strain was purified and characterized and tested for food additive applications. Monophenolase and diphenolase activitie...

Wheat and oilseed flax straws were studied as raw material for papermaking. Two different aspects were investigated to valorise these agricultural by-products: capacity to recover phenolic compounds and cellulose fibres valorisation to papermaking. Ferulic acid, coumaric acid, vanillic acid and vanillin were found in both wheat and flax straws and...

Referencia OEPM: P200102804.-- Fecha de solicitud: 17/12/2001.-- Titulares: Consejo Superior de Investigaciones Científicas (CSIC), Universidad Politécnica de Cataluña, Institut National de la Recherche Agronomique-France. Esta invención describe un procedimiento para el blanqueo de pastas de papel de alta calidad obtenidas a partir de plantas herb...

Three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. Laccase from Pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. The corresponding recombinant laccases were produced in Aspergillus oryzae...

A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l(-1...

Laboratory experiments were conducted to study the potential of adding gaseous toluene, as a readily degradable carbon source, to enhance phenanthrene mineralization in polluted soil (1,000 mg/kg(dry soil)) aged for 400 days. Experiments were conducted in 0.5-L column reactors packed with a mixture of (80:20 w(wet)/w(wet)) spiked soil and vermiculi...

Laccase production by the monokaryotic strain Pycnoporus cinnabarinus ss3 was studied using ethanol as inducer in the culture medium. The effect of ethanol was tested at 10, 20, 30, 35 and 45 g l-1 and compared with that of ferulic acid, known until now as the most efficient inducer for laccase expression by P. cinnabarinus ss3. In the presence of...

The development and use of biotechnology in the pulp and paper industry started in the early 1970s and depends mainly on the production of inexpensive biocatalysts in enormous volumes and weights for industrial applications. Some biotechnical processes have already been implemented in the industry, such as bio-bleaching with xylanases, pitch reduct...

A laccase-mediator system was applied on pre-treatment of wheat-straw pulps. The characteristics of enzyme treated pulps and their bleaching abilities by several TCF bleaching sequences were investigated. The results show that bio-treatment of laccase-mediator system can enhances not only optical properties but also physical strength of the treated...

This study investigated the lignolytic and hemicellulolytic pathways of a monokaryotic Pycnoporuscinnabarinus strain (ss3) hyperproducing laccase. The extracellular enzymes produced were identified as xylanase, laccase and cellobiose dehydrogenase (CDH). Their productions were examined using different natural substrates: cellulose, sugar beet pulp,...

The production of laccase, an enzyme of industrial interest, was screened among species of the genus Pycnoporus, in particular P. sanguineus. Strains were isolated from various tropical Chinese environments and phylogenetically compared to ones deposited in international collections. Molecular clustering, based on ribosomal ITS1–5.8S-ITS2 genomic s...

The treatment of wheat straw chemical pulp combining xylanases and laccase mediator system (LMS) followed by alkaline extraction reduced the lignin content by about 60%. Enzymes were applied sequentially as a pretreatment in hypochlorite (H) and in TCF bleaching sequences. The enzymatic treatment of wheat straw pulp reduced the consumption of chlor...

Laccase isozymes from the white-rot basidiomycete fungi Trametes versicolor and Pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. T. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group P2(1)2(1)2(1), which diffract to 1.9 and 2.95 A resolution, respectively. The cry...

The biotransformation of 16 polycyclic aromatic hydrocarbons (PAHs) in mixture was investigated in reactors in the presence of purified laccases of the fungus Pycnoporus cinnabarinus, ABTS as a redox mediator, 25% acetonitrile, and Tween 20. Several hydrocarbons from a synthetic mixture, such as anthracene and benzo[ a ]pyrene, were converted up to...

Olive oil residues were tested for their composition in simple phenolic compounds as a function of the extraction system, i.e. the three- and two-phase centrifugation systems. Phenolic compound extraction with ethyl acetate was efficient and allowed recovery of 28.8 and 42.2% of total phenols present in dry olive oil residues originating from three...

An alkaline peroxide industrial pulp from poplar was treated with a manganese peroxidase (MnP) from the hypersecretory strain of Phanerochaete chrysosporium I-1512 after a second stage of refining. The enzymatic treatment caused an improvement in the pulp quality by inducing an enzymatic refining onto the fibers. Transmission electron microscopy sh...

Solid carriers have been developed to inoculate Trametes versicolor and Cunninghamella elegans into manufactured gas plant site soils. Pelleted wheat bran carriers were very efficient in stimulating the growth of fungi in an industrial soil containing about 2800 mg kg PAHs. Fungal biomass and activity of extracellular laccases, enzymes produced by...

The objective of this European project (AIR3-CT94-2065) was to improve the quality of an industrial poplar high-yield pulp by introducing an treatment with a crude enzyme preparation enriched in the ligninolytic manganese peroxidases (MnP). The enzymes used in this study were produced from an hypersecretory strain of Phanerochaete chrysosporium. Op...