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Jean-Claude Mollet

Jean-Claude Mollet
Normandie Univ, UNIRouen · Biology

PhD

About

81
Publications
13,690
Reads
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2,110
Citations
Introduction
Jean-Claude Mollet currently works at the Biology, Normandie Univ, UNIRouen. Jean-Claude does research in Cell Biology, Physiology and Agricultural Plant Science. Their current project is 'Plant cell wall glycomolecules and cell growth'.
Additional affiliations
September 2007 - present
Université de Rouen
Position
  • Professor (Full)
September 2003 - August 2007
Université d'Artois
Position
  • Professor (Associate)
February 1998 - August 2003
University of California, Riverside
Position
  • PostDoc Position
Education
September 1989 - December 1993
Université de Technologie de Compiègne
Field of study
  • Cell wall regeneration of red macroalgae protoplasts

Publications

Publications (81)
Article
Full-text available
UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides, and is required for production of seed mucilage. Following synthesis in the cytosol, it is transported into the lumen of the Golgi apparatus, where it is converted to UDP-galacturonic acid (UDP-GalA), UDP-arabinose and UDP-xylose. To identify the Golgi-localize...
Article
In plants, 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) is a monosaccharide that is only found in the cell wall pectin, rhamnogalacturonan-II (RG-II). Incubation of 4-day-old light-grown Arabidopsis seedlings or tobacco BY-2 cells with 8-azido 8-deoxy Kdo (Kdo-N3 ) followed by coupling to an alkyne-containing fluorescent probe resulted in the specific...
Article
Tip growth is a common strategy for the rapid elongation of cells to forage the environment and/or to target to long-distance destinations. In the model tip growth system of Arabidopsis pollen tubes, several smallmolecule hormones regulate their elongation, but how these rapidly diffusing molecules control extremely localized growth remains mysteri...
Article
Full-text available
Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis thaliana pollen. A combination of functional genomics, gene expression, in vivo and in vitro po...
Article
Full-text available
The pollen tube is a fast tip-growing cell carrying the two sperm cells to the ovule allowing the double fertilization process and seed setting. To succeed in this process, the spatial and temporal controls of pollen tube growth within the female organ are critical. It requires a massive cell wall deposition to promote fast pollen tube elongation a...
Article
Investigation of protein tracking in living plant cells has became a routine experiment with the emergence of reporter genes encoding fluorescent tags. Unfortunately, this imaging strategy is not applicable to glycans because their synthesis is not directly encoded by the genome. Indeed, complex glycans result from sequential additions and/or remov...
Preprint
Pollen tubes live a life on a razor′s edge. They must maintain cell wall integrity whilst growing towards the ovule at extraordinary speed but explosively burst at just the right moment to release the sperm cells—with fatal consequences for reproduction if things go wrong. The precisely controlled growth of the pollen tube depends on the fine-tuned...
Article
Full-text available
To date, it is widely accepted by the scientific community that many agricultural regions will experience more extreme temperature fluctuations. These stresses will undoubtedly impact crop production, particularly fruit and seed yields. In fact, pollination is considered as one of the most temperature-sensitive phases of plant development and until...
Article
Ethylene modulates plant developmental processes including flower development. Previous studies have suggested ethylene participation in pollen tube (PT) elongation, and both ethylene production and perception seem critical at fertilization time. The full gene set regulated by ethylene during PT growth is unknown. To study this, we used various ETh...
Article
Sexual selection is known to shape plant traits that affect access to mates during the pollination phase, but it is less well understood to what extent it affects traits relevant to interactions between pollen and pistils after pollination. This is surprising, because both of the two key modes of sexual selection, male–male competition and female c...
Article
Plants are surrounded by a diverse range of microorganisms that causes serious crop losses and requires the use of pesticides. Flax is a major crop in Normandy used for its fibres and is regularly challenged by the pathogenic fungus Fusarium oxysporum (Fo) f. sp. lini. To protect themselves, plants use “innate immunity” as a first line of defense l...
Article
In Normandy, flax is a plant of important economic interest because of its fibers. Fusarium oxysporum, a telluric fungus, is responsible for the major losses in crop yield and fiber quality. Several methods are currently used to limit the use of phytochemicals on crops. One of them is the use of Plant Growth Promoting Rhizobacteria (PGPR) occurring...
Article
The objective of this study was to investigate a possible biological activities of oligosaccharides resulting from the hydrolysis of parietal hemicelluloses of the foliar tissue of Aristida pungens. Known for its developed, fiber rich foliar tissue, this perennial grasses is widely distributed on the arid and sub-desertic regions of Algeria. In ord...
Article
Plant cell wall remodeling plays a key role in the control of cell elongation and differentiation. In particular, the fine‐tuning of the degree of methylesterification of pectins was previously reported to control developmental processes as diverse as pollen germination, pollen tube elongation, primordia emergence or dark‐grown hypocotyl elongation...
Article
Full-text available
Background During sexual reproduction, pollen grains land on the stigma, rehydrate and produce pollen tubes that grow through the female transmitting-tract tissue allowing the delivery of the two sperm cells to the ovule and the production of healthy seeds. Because pollen tubes are single cells that expand by tip-polarized growth, they represent a...
Article
Full-text available
During evolution of land plants, the first colonizing species presented leafy-dominant gametophytes, found in non-vascular plants (bryophytes). Today, bryophytes include liverworts, mosses, and hornworts. In the first seedless vascular plants (lycophytes), the sporophytic stage of life started to be predominant. In the seed producing plants, gymnos...
Article
Full-text available
O‐Acetylated pectins are abundant in the primary cell wall of plants and growing evidence suggests they have important roles in plant cell growth and interaction with the environment. Despite their importance, genes required for O‐acetylation of pectins are still largely unknown. In this study, we showed that TRICHOME BIREFRINGENCE LIKE 10 (AT3G060...
Article
Full-text available
Main conclusion: A chemical screen of plant-derived compounds identified holaphyllamine, a steroid, able to trigger defense responses in Arabidopsis thaliana and improve resistance against the pathogenic bacterium Pseudomonas syringae pv tomato DC3000. A chemical screen of 1600 plant-derived compounds was conducted and allowed the identification o...
Article
Full-text available
AtPME3 (At3g14310) is a ubiquitous cell wall pectin methylesterase. Atpme3-1 loss-of-function mutants exhibited distinct phenotypes from the wild type (WT), and were characterized by earlier germination and reduction of root hair production. These phenotypical traits were correlated with the accumulation of a 21.5-kDa protein in the different organ...
Article
Full-text available
The fine-tuning of the degree of methylesterification of cell wall pectin is a key to regulate cell elongation and ultimately the shape of plant body. Pectin methylesterification is spatio-temporally controlled by pectin methylesterases (PMEs, 66 members in Arabidopsis). The comparably large number of proteinaceous pectin methylesterase inhibitors...
Article
Tip growth is a common strategy for the rapid elongation of cells to forage the environment and/or to target to long-distance destinations. In the model tip growth system, Arabidopsis pollen tubes, several small molecule hormones regulate their elongation, but how these rapidly-diffusing molecules control extremely localized growth remains mysterio...
Article
Full-text available
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of th...
Article
Screening of commercially available fluoro monosaccharides as putative growth inhibitors in Arabidopsis thaliana revealed that 2-fluoro 2-L-fucose (2F-Fuc) reduces root growth at micromolar concentrations. The inability of 2F-Fuc to affect an Atfkgp mutant that is defective in the fucose salvage pathway indicates that 2F-Fuc must be converted to it...
Article
Screening of commercially available fluoro monosaccharides as putative growth inhibitors in Arabidopsis thaliana revealed that 2-fluoro 2-L-fucose (2F-Fuc) reduces root growth at micromolar concentrations. The inability of 2F-Fuc to affect an Atfkgp mutant that is defective in the fucose salvage pathway indicates that 2F-Fuc must be converted to it...
Article
Full-text available
Retention of metal cations by the cell wall is a common process found in plants in response to stress induced by the presence of trace metals (TMs). In this study conducted on a tomato cell suspension culture, cadmium (Cd) and zinc (Zn) were added to the medium at maximal concentrations of 0.5 and 2 mM, respectively. We showed that around 50 % of Z...
Article
Full-text available
Two independent studies have shown that the cell wall of pollen tubes from tobacco and tomato species contained fucosylated xyloglucan (XyG). These findings are intriguing as many reports have shown that XyG of somatic cells of these species is not fucosylated but instead is arabinosylated. In order to produce fucosylated XyG, plants must express a...
Article
Full-text available
Background and Aims In flowering plants, fertilization relies on the delivery of the sperm cells carried by the pollen tube to the ovule. During the tip growth of the pollen tube, proper assembly of the cell wall polymers is required to maintain the mechanical properties of the cell wall. Xyloglucan (XyG) is a cell wall polymer known for maintainin...
Article
Full-text available
Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo a...
Article
] The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure resu...
Article
] Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 hold Arabidopsis pollen tub...
Article
Full-text available
Background and Aims In flowering plants, fertilization relies on the delivery of the sperm cells carried by the pollen tube to the ovule. During the tip growth of the pollen tube, proper assembly of the cell wall polymers is required to maintain the mechanical properties of the cell wall. Xyloglucan (XyG) is a cell wall polymer known for maintainin...
Article
Full-text available
Background and Aims Rhamnogalacturonan-II (RG-II) is one of the pectin motifs found in the cell wall of all land plants. It contains sugars such as 2-keto-3-deoxy-d-lyxo-heptulosaric acid (Dha) and 2-keto-3-deoxy-d-manno-octulosonic acid (Kdo), and within the wall RG-II is mostly found as a dimer via a borate diester cross-link. To date, little is...
Article
Full-text available
Pectins are major components of primary cell wall that play a crucial role in plant development. After biosynthesis, pectins are secreted in the cell wall by Golgi-derived vesicles under a highly methylesterified form and are de-methylesterified by pectin methylesterases (PME). It is hypothesized that PME might be regulated by pectin methylesterase...
Article
Date palm (Phoenix dactylifera) is an important crop providing a valuable nutrition source for people in many countries including the Middle East and North Africa. In recent years, the amount of rain in North Africa and especially in the Tunisian palm grove areas has dropped significantly. We investigated the growth and cell wall remodeling of frui...
Article
Full-text available
Background Arabinogalactan proteins (AGPs) are complex proteoglycans of the cell wall found in the entire plant kingdom and in almost all plant organs. AGPs encompass a large group of heavily glycosylated cell-wall proteins which share common features, including the presence of glycan chains especially enriched in arabinose and galactose and a prot...
Article
Full-text available
Plant sexual reproduction involves the growth of tip-polarized pollen tubes through the female tissues in order to deliver the sperm nuclei to the egg cells. Despite the importance of this crucial step, little is known about the molecular mechanisms involved in this spatial and temporal control of the tube growth. In order to study this process and...
Article
Full-text available
During plant sexual reproduction, pollen germination and tube growth require development under tight spatial and temporal control for the proper delivery of the sperm cells to the ovules. Pollen tubes are fast growing tip-polarized cells able to perceive multiple guiding signals emitted by the female organ. Adhesion of pollen tubes via cell wall mo...
Article
Full-text available
Aristida pungens, a perennial grass widely distributed on the Algerian arid regions, may potentially be the source of raw cell wall material to promote a local development in North Africa. Hemicelluloses, the main matrix cell wall polymers of this species were isolated from leaves with 14% KOH aqueous solution, characterized and converted as plasti...
Article
Full-text available
Lily pollen tubes grow adhering to an extracellular matrix produced by the transmitting tract epidermis in a hollow style. SCA, a small ( approximately 9.4 kDa), basic protein plus low esterified pectin from this extracellular matrix are involved in the pollen tube adhesion event. The mode of action for this adhesion event is unknown. We partially...
Chapter
IntroductionPollen formation and microspore separationPollen-stigma adhesion and pollen tube guidanceAdhesion and guidance of pollen tubes in the styleCell wall modifying proteins and pollen tube growth in the ECMPollen tube adhesion, interaction and guidance in the ovaryConclusions and perspectivesAcknowledgements
Article
Full-text available
Activities of two de-N-glycosylation enzymes, PNGase (peptide N 4(N-acetyl-glucosaminyl)asparagine amidase) and ENGase (endo N-acetyl-β-D-glucosaminidase), involved in the release of N-glycans from N-glycoproteins, were monitored in several organs of tomato plants (Lycopersicon esculentum, Mill., cv. Dombito) with a fluorescence-HPLC procedure usin...
Article
Full-text available
Gigartina pistillata (Gmelin) Stackhouse harvested commercially along the Atlantic coast of Morocco is generally a mixture of gametophytes with a cell wall enriched in κ-carrageenan, and tetrasporophytes with a wall composed mainly of λ-carrageenan. In order to separate both polysaccharides, two extraction methods were investigated. The first one i...
Article
Full-text available
The style of lily produces a specialized extracellular matrix (ECM) in the transmitting tract epidermis that functions to guide pollen tubes to the ovary. This adhesive ECM contains low esterified pectins and a peptide, SCA (stigma/stylar cysteine-rich adhesin). Together they form a matrix to which pollen tubes adhere as they grow through the style...
Article
Full-text available
In plant reproduction, pollination is an essential process that delivers the sperm through specialized extracellular matrices (ECM) of the pistil to the ovule. Although specific mechanisms of guidance for pollen tubes through the pistil are not known, the female tissues play a critical role in this event. Many studies have documented the existence...