Jean-Charles Martin

Jean-Charles Martin
Aix-Marseille Université | AMU · Faculté de Médecine

PhD
heading the MS based metabolomics/lipidomics platform for investigating human health and well-being

About

150
Publications
20,849
Reads
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Introduction
Jean-Charles Martin currently works at the Centre for CardioVascular and Nutrition (C2VN), French National Institute for Agricultural Research. Jean-Charles does research in Biostatistics, Physiology and Systems Biology. He is using the metabolomic approach to understand the environmental influence, including nutrition, on health, disease and well being'.
Additional affiliations
January 2014 - present
Aix-Marseille Université
Position
  • Managing Director
September 2004 - present
Aix-Marseille Université
Position
  • Lecturer
April 1999 - September 2004
Université Paris-Sud 11
Position
  • Professor (Assistant)

Publications

Publications (150)
Article
Full-text available
The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11 different mass spectrometers with the objective of assessing the reliability of untargeted metabolomics approaches in obtaining comparable metabolomics profiles. This was estimated by measuring the proportion of common spectral information extracted...
Article
Full-text available
Changes in metabolomics over time were studied in rats to identify early biomarkers and highlight the main metabolic pathways that are significantly altered in the period immediately following acute low-dose uranium exposure. A dose response relationship study was established from urine and plasma samples collected periodically over 9 months after...
Article
Full-text available
On one side, decompression sickness (DCS) with neurological disorders lead to a reshuffle of the cecal metabolome of rats. On the other side, there is also a specific and different metabolomic signature in the cecum of a strain of DCS-resistant rats, that are not exposed to hyperbaric protocol. We decide to study a conventional strain of rats that...
Article
Full-text available
Purpose To evaluate the effectiveness of vitamin D3 supplementation, in secondary prevention, on cardiac remodeling and function, as well as lipid profile, in a mouse model of diet-induced type 2 diabetes. Methods Mice were fed a high fat and sucrose diet for 10 weeks. Afterward, diet was maintained for 15 more weeks and two groups were formed, wi...
Article
Résumé L’obésité réduit la perception oro-sensorielle des lipides alimentaires chez les rongeurs et chez certains sujets, affectant ainsi leurs choix alimentaires. Bien que la chirurgie bariatrique corrige partiellement cette altération, les mécanismes sous-jacents restent mal compris. Pour explorer l’implication potentielle de changements métaboli...
Article
Introduction et but de l’étude L’obésité nutritionnelle réduit la perception orosensorielle des lipides alimentaires chez les rongeurs et chez les certains sujets impactant ainsi leurs choix alimentaires. Bien que la chirurgie bariatrique corrige partiellement cette altération, les mécanismes sous-jacents restent mal compris. Matériel et méthodes...
Article
Full-text available
The vascular endothelium is a hot spot in the response to radiation therapy for both tumors and normal tissues. To improve patient outcomes, interpretable systemic hypotheses are needed to help radiobiologists and radiation oncologists propose endothelial targets that could protect normal tissues from the adverse effects of radiation therapy and/or...
Article
Full-text available
Macrophages rely on tightly integrated metabolic rewiring to clear dying neighboring cells by efferocytosis during homeostasis and disease. Here we reveal that glutaminase-1-mediated glutaminolysis is critical to promote apoptotic cell clearance by macrophages during homeostasis in mice. In addition, impaired macrophage glutaminolysis exacerbates a...
Article
Full-text available
Background Excess visceral obesity and ectopic organ fat is associated with increased risk of cardiometabolic disease. However, circulating markers for early detection of ectopic fat, particularly pancreas and liver, are lacking. Methods Lipid storage in pancreas, liver, abdominal subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT)...
Article
Objectives We aimed to characterize the effects of a low dose of a sustained-release L-arginine (L-ARG) supplementation on plasma metabolome in healthy overweight adults with cardiometabolic risk factors, and study if its effect could improve the capacity to handle a high fat-high sugar meal, that deeply and acutely challenge metabolic capacities....
Article
Full-text available
Objective: Dietary intakes must cover protein and essential amino acid (EAA) requirements. For this purpose, different methods have been developed such as the nitrogen balance method, factorial method, or AA tracer studies. However, these methods are either invasive or imprecise, and the Food and Agriculture Organization of the United Nations (FAO...
Article
Full-text available
Diet-induced obesity (DIO) reduces the orosensory perception of lipids in rodents and in some humans. Although bariatric surgery partially corrects this alteration, underlying mechanisms remain poorly understood. To explore whether metabolic changes might explain this fat taste disturbance, plasma metabolome analyses, two-bottle choice tests and fu...
Article
Full-text available
On one side, decompression sickness (DCS) with neurological disorders lead to a reshuffle of the fecal metabolome from rat caecum. On the other side, there is high inter-individual variability in terms of occurrence of DCS. One could wonder whether the fecal metabolome could be linked to the DCS-susceptibility. We decided to study male and female r...
Presentation
Full-text available
Recent advances in MS Lipidomics shed light to new territories of biological regulations, like the hubble telescope showing unexplored regions of deep space. To illustrate this, in a first study we examined the lipidome of epicardial adipose tissue (an active endocrine organ) and compared it to subcutaneous adipose tissue in patients with coronary...
Article
Full-text available
Beside oil, oilseed rape (Brassica napus) seeds contains nutritional bioactives such as polyphenols and glucosinolates. However, to date their nutritional properties have been overlooked in the new “double zero” breeds. Seed alcoholic extracts from two B.napus cultivars most contrasting in their phytochemical contents as measured by mass-spectromet...
Article
Full-text available
Background Asian Chinese are more susceptible to deposition of visceral adipose tissue (VAT) and type 2 diabetes (T2D) development than European Caucasians when matched for gender, age and body mass index (BMI). Our aims were: (i) characterise the ethnicity-specific metabolomic signature of visceral adiposity measured by dual energy X-ray absorptio...
Article
Full-text available
A role of the gut microbiota in psychiatric disorders is supported by a growing body of literature. The effects of a probiotic mixture of four bacterial strains were studied in two models of anxiety and depression, naturally stress-sensitive Fischer rats and Long Evans rats subjected to maternal deprivation. Rats chronically received either the pro...
Article
Full-text available
Massive bubble formation after diving can lead to decompression sickness (DCS), which can result in neurological disorders. We demonstrated that hydrogen production from intestinal fermentation could exacerbate DCS in rats fed with a standard diet. The aim of this study is to identify a fecal metabolomic signature that may result from the effects o...
Preprint
Full-text available
Macrophages rely on tightly integrated metabolic rewiring to clear dying neighboring cells by efferocytosis during homeostasis and disease. Here, we reveal glutaminase (Gls) 1-mediated glutaminolysis is critical to promote apoptotic cell clearance by macrophages during homeostasis. In addition, impaired macrophage glutaminolysis exacerbated atheros...
Article
Full-text available
Objective: Deep vein thrombosis and pulmonary embolism referred as venous thromboembolism (VTE) are a common cause of morbidity and mortality. Plasma from healthy controls or individuals who have experienced a VTE were analyzed using metabolomics to characterize biomarkers and metabolic systems of patients with VTE. Approach and Results: Polar met...
Article
Genipap (Genipa americana L.) is an exotic fruit largely consumed and well known, in Amazonian pharmacopeia, to treat anemia, measles and uterine cancer. It is also used as a diuretic, digestive, healing, laxative and antiseptic. The aim of this study was to apply an untargeted metabolomics strategy to determine biomarkers of food intake after shor...
Preprint
Full-text available
Obesity is a major issue in pets and nutritional strategies need to be developed, like promoting greater protein and fiber intake. This study aimed to evaluate the effects of dietary protein levels and prebiotic supplementation on the glucose metabolism and relationships between the gut, microbiota, metabolome, and phenotype of obese dogs. Six obes...
Article
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The long-lasting consequence of a new iodine thyroid blocking strategy (ITB) to be used in case of nuclear accident is evaluated in male Wistar rats using a metabolomics approach applied 30 days after ITB completion. The design used 1 mg/kg/ day of KI over 8 days. Thyroid hormones remained unchanged, but there was a metabolic shift measured mainly...
Article
Full-text available
Objective: Epicardial adipose tissue (EAT) is an active endocrine organ that could contribute to the pathophysiology of coronary artery disease (CAD) through the paracrine release of proatherogenic mediators. Numerous works have analyzed the inflammatory signature of EAT, but scarce informations on its lipidome are available. Our objective was fir...
Article
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Milk has a specific saturated fatty acid profile and its calcium content may change the kinetics of fat absorption. The goal of this study was to compare the effect on LDL Cholesterol and other risk markers of four isolipidic diets differing by their fat food source, vegetable fat, spring milk fat, winter milk fat or winter milk fat supplemented wi...
Article
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Background Several lipid metabolites in cerebrospinal fluid are correlated with poor outcomes in aneurysmal subarachnoid haemorrhage. Most of these metabolites bind to ubiquitous thromboxane–prostaglandin (TP) receptors, causing vasoconstriction and inflammation. Here, we evaluated terutroban (TBN), a specific TP receptor antagonist, for the preven...
Article
Full-text available
Vitamin D metabolism is actively modulated in adipose tissue during obesity. To better investigate this process, we develop a specific LC-HRMS/MS method that can simultaneously quantify three vitamin D metabolites, i.e., cholecalciferol, 25-hydroxyvitamin D3 (25(OH)D3), and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) in a complex matrix, such as mouse a...
Article
Full-text available
This study was designed to elucidate the biological variation in expression of many metabolites due to environment, genotype, or both, and to investigate the potential utility of metabolomics to supplement compositional analysis for the design of a new resilient cultivar of Brassica napus that can be steady in phytochemicals in different regions in...
Article
Full-text available
Preparedness for nuclear accident responsiveness includes interventions to protect pregnancies against prolonged exposure to radioactive iodine. The aim of this study was to investigate a new design consisting of repeated administration of potassium iodide (KI, 1 mg/kg) for 8 days in late pregnancy gestational day 9-16 (GD9-GD16) in rats. The later...
Poster
Full-text available
Introduction Chez le patient diabétique de type 2 (DT2), l’hypovitaminose D a été associée au risque cardiovasculaire. Des études menées dans des modèles de DT1 ont rapporté les effets cardioprotecteurs de la vitamine D (VitD). Cependant, les données dans des modèles de DT2 induits par régime font encore défaut. La lipotoxicité fait partie des proc...
Article
Résumé Les formules infantiles (FI) miment la composition du lait maternel, considéré comme le « gold standard ». Depuis les années 60, la composition lipidique du lait maternel a évolué, suite aux modifications du régime de la population générale et des mères, qui ont remplacé les acides gras saturés (AGS), déclarés pro-athérogènes, par des AGPIn-...
Article
Full-text available
Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nontoxic solution of uranyl nitrate...
Article
Full-text available
The Fukushima nuclear power plant blast resulted in the release of ¹³¹ Iodine for several weeks. This unexpected issue challenged the iodin doctrine [ bibrR11 ], in which the counter-measure is to provide a unique iodine tablet to saturate thyroid during the radioactive contamination not expected to last more than several hours. A new doctrine must...
Article
Trans-resveratrol is a stilbene polyphenol with a large spectrum of biological activities. This is why it is widely studied in terms of activities, bioavailability and quantitation in different foods, beverages and biological matrices. Different analytical methods are employed for its quantitation. In this study a quadrupole-orbitrap tandem mass sp...
Article
Full-text available
Single dose of potassium iodide (KI) is recommended to prevent the risk of thyroid cancer during nuclear accidents. However in the case of repeated/protracted radioiodine release, a unique dose of KI may not protect efficiently the thyroid against the risk of further developing a radiation-induced cancer. The new WHO guidelines for the use in plann...
Article
Full-text available
Abstract: Genipap (Genipa americana L.) is a native fruit from Amazonia that contains bioactive compounds with a wide range of bioactivities. However, the response to genipap juice ingestion in the human exposome has never been studied. To identify biomarkers of genipap exposure, the untargeted metabolomics approach in human urine was applied. Urin...
Article
Full-text available
Like other tumors, aggressive soft tissue sarcomas (STS) use glycolysis rather than mitochondrial oxidative phosphorylation (OXPHOS) for growth. Given the importance of the cofactor coenzyme A (CoA) in energy metabolism, we investigated the impact of Vnn1 pantetheinase—an enzyme that degrades pantetheine into pantothenate (vitamin B5, the CoA biosy...
Article
Low circulating levels of total and free 25-hydroxyvitamin D (25(OH)D) indicative of vitamin D status have been associated with obesity in humans. Moreover, obesity is thought to play a causal role in the reduction of 25(OH)D levels, and several theories have been put forward to explain this relationship. Here we tested the hypothesis that obesity...
Presentation
Full-text available
presentation video of the CRIBIOM/BIOMET platform
Article
Full-text available
Recommendations for infant formulas had been established on the basis of human breast milk composition, still considered as “the gold standard”. However changes occurred since the sixties that modified lipid composition of human breast milk which is correlated to the diet of the mothers, who increased n-6 PUFA consumption to replace saturated fat c...
Article
Full-text available
We evaluated the effect of adding docosahexaenoic:arachidonic acids (3:2) (DHA+ARA) to 2 representative commercial infant formulas on brain activity and brain and eye lipids in an artificially reared rat pup model. The formula lipid background was either a pure plant oil blend, or dairy fat with a plant oil blend (1:1). Results at weaning were comp...
Article
It is well established that the active form of vitamin D, i.e. 1,25(OH)2D, regulates the expression of genes involved in its own metabolism and transport in the kidney, and possibly in the liver. However, little is known about the transcriptional impact of cholecalciferol supplementation on white adipose tissue (WAT) and adipocytes, which are a maj...
Conference Paper
Full-text available
Introduction: Cats are fed with high protein diets that can lead to the production of high levels of catabolites derived from amino-acids fermentation. Such catabolites can be associated with malodorous faeces and several diseases. A dietary supplementation with short-chain fructooligosaccharides (scFOS) defined as prebiotic fibres may be of intere...
Article
Full-text available
Introduction et but de l’étude Le cerveau du nourrisson, immature à la naissance, est sensible à la qualité des apports en acides gras polyinsaturés. L’objectif de cette étude était d’évaluer l’impact de la qualité lipidique de formules infantiles sur la composition et le fonctionnement du cerveau chez le raton avant sevrage. Matériel et méthodes...
Article
Full-text available
IntroductionData are sparse about the potential health risks of chronic low-dose contamination of humans by uranium (natural or anthropogenic) in drinking water. Previous studies report some molecular imbalances but no clinical signs due to uranium intake. Objectives In a proof-of-principle study, we reported that metabolomics is an appropriate met...
Article
Background: Delayed cerebral ischaemia from vasospasm is an important cause of complications and death after aneurysmal subarachnoid haemorrhage. There is currently no established biomarker for identifying patients at high risk of delayed cerebral ischaemia. Objective: Considering the important role of inflammation in the pathogenesis of delayed...
Article
Aim: Ectopic adiposity has been associated with type 2 diabetes (T2D) complications. Glucagon-like-peptide-1 analogues are currently used in T2D, but no prospective randomized trial has investigated their effect on ectopic fat stores. Research design and methods 44 obese type 2 diabetics uncontrolled on oral-antidiabetic-drugs were randomly assign...
Article
Full-text available
Natural uranium (NU), a component of the earth’s crust, is not only a heavy metal but also an alpha particle emitter, with chemical and radiological toxicity. Populations may therefore be chronically exposed to NU through drinking water and food. Since the central nervous system is known to be sensitive to pollutants during its development, we asse...
Article
Full-text available
Objective: There is substantial inter-individual diversity in the susceptibility of alcoholics to liver injury. Alterations of intestinal microbiota (IM) have been reported in alcoholic liver disease (ALD), but the extent to which they are merely a consequence or a cause is unknown. We aimed to demonstrate that a specific dysbiosis contributes to...
Article
Full-text available
We assessed the atheroprotective efficiency of modified dairy fats in hyperlipidemic hamsters. A systems biology approach was implemented to reveal and quantify the dietary fat-related components of the disease. Three modified dairy fats (40% energy) were prepared from regular butter by mixing with a plant oil mixture, by removing cholesterol alone...
Article
Full-text available
Le lait maternel apporte au nourrisson l’équivalent de 5 à 10 fois la quantité de cholestérol de celle d’un adulte. En comparaison, les laits infantiles historiquement fabriqués à base de graisses laitières apportaient également du cholestérol au nourrisson mais en quantité moindre en raison d’une dilution avec les graisses végétales utilisées pour...